Printing Pressure Research Articles

Calcium supplementation of bioinks reduces shear stress-induced cell damage during bioprinting

During bioprinting, cells are suspended in a viscous bioink and extruded under pressure through small diameter printing needles. The combination of high pressure and small needle diameter exposes cells to considerable shear stress, which can lead to cell damage and death. Approaches to monitor and control shear stress-induced cell damage are currently not well established. To visualize the effects of printing-induced shear stress on plasma membrane integrity, we add FM 1-43 to the bioink, a styryl dye that becomes fluorescent when bound to lipid membranes, such as the cellular plasma membrane. Upon plasma membrane disruption, the dye enters the cell and also stains intracellular membranes. Extrusion of alginate-suspended NIH/3T3 cells through a 200 µm printing needle led to an increased FM 1-43 incorporation at high pressure, demonstrating that typical shear stresses during bioprinting can transiently damage the plasma membrane. Cell imaging in a microfluidic channel confirmed that FM 1-43 incorporation is caused by cell strain. Notably, high printing pressure also impaired cell survival in bioprinting experiments. Using cell types of different stiffnesses, we find that shear stress-induced cell strain, FM 1-43 incorporation and cell death were reduced in stiffer compared to softer cell types and demonstrate that cell damage and death correlate with shear stress-induced cell deformation. Importantly, supplementation of the suspension medium with physiological concentrations of CaCl2 greatly reduced shear stress-induced cell damage and death but not cell deformation. As the sudden influx of calcium ions is known to induce rapid cellular vesicle exocytosis and subsequent actin polymerization in the cell cortex, we hypothesize that calcium supplementation facilitates the rapid resealing of plasma membrane damage sites. We recommend that bioinks should be routinely supplemented with physiological concentrations of calcium ions to reduce shear stress-induced cell damage and death during extrusion bioprinting.

Extrusion 3D printing of keratin protein hydrogels free of exogenous chemical agents

Keratins are a class of intermediate filament proteins that can be obtained from numerous sources including human hair. Materials fabricated from keratins offer desirable characteristics as scaffolds for tissue engineering, including intrinsic cell adhesion sequences and tunable degradation kinetics. The capacity to create 3D printed constructs from keratin-based bio-inks generates unique opportunities for spatial control of scaffold physicochemical properties to direct scaffold functions in ways not readily achieved through other means. The aim of this study was to leverage the controllable rheological properties of keratin hydrogels to create a strategy for extrusion 3D printing of keratin bio-inks without the use of exogenous rheological modifiers, crosslinking agents, or photocurable resins. The rheological properties of keratin hydrogels were tuned by varying two parameters: (a) the ratio of keratose (obtained by oxidative extraction of keratin) to kerateine (obtained by reductive extraction of keratin); and (b) the weight percentage of total keratin protein in the gel. A computational model of the dispensing nozzle for a commercially available extrusion 3D printer was developed to calculate the needed pneumatic printing pressures based on the known rheological properties of the gels. Keratin hydrogel constructs, of varying keratose/kerateine ratios and total keratin weight percentages, were 3D printed in cylindrical geometries via extrusion 3D printing. Rheology and degradation studies showed that gels with greater relative kerateine content exhibited greater flow resistance and slower degradation kinetics when submerged in phosphate buffered saline solution at 37 °C, owing to the presence of cysteine residues in kerateine and the capability of forming disulfide bonds. Total keratin weight percentage was found to influence gel yield stress, with possible implications for tuning filament fidelity. Findings from this work support the use of keratose/kerateine ratio and total keratin weight percentage as handles for modulating rheological characteristics of keratin hydrogels to enhance printability and control scaffold properties.

Numerical simulation of the water-based ink transfer process in roll-to-roll gravure printing based on fluid–solid interactions

The roll-to-roll gravure printing ink transfer process plays an important role in enhancing printing quality and saving on costs. The static analysis and fluid–solid interaction method are used for the first time to conduct a systematic study on the coupling between the fluid flow state and the solid deformation in the whole process of roll-to-roll gravure printing. The static compression stage, the initial moment of ink transfer, and the process of ink passing through the gap between two rollers and the separation of the ink layer with the rotation of two rollers are considered. The effect of ink layer thickness on the ink transfer process is studied. At a printing pressure of 0.2 MPa and a printing velocity of 200 rpm, the ink thickness has a great influence on the ink flow state, which leads to backflow; the phenomenon causes irregularities in the effective ink transfer ratio. The critical ink layer thickness is 70 µm under the above gravure printing conditions. This should not be exceeded to ensure the stability of ink transfer. Under the above printing conditions, when the ink layer thickness is in the range of 30–50 µm, there is no ink backflow phenomenon and the width of the ink flow channel is relatively large, and the effective ink ratio is almost stable at 50%. This study is helpful for controlling the ink quantity in the gravure printing, providing strong theoretical support for the improvement of the gravure printing process, and promoting the application of the water-based ink.

A Heating-Assisted Direct Ink Writing Method for Preparation of PDMS Cellular Structure with High Manufacturing Fidelity.

In response to the fact that most of the current research on silicone 3D printing suffers from structure collapse and dimensional mismatch, this paper proposes a heating-assisted direct writing printing method for commercial silicone rubber materials for preparing silicone foam with enhanced fidelity. In the experimental processes, the effects of substrate temperature, printing pressure, and printing speed on the filament width were investigated using a controlled variable method. The results showed the following: (1) the diameter of silicone rubber filaments was positively correlated with the printing pressure and substrate temperature, but negatively correlated with the printing speed; (2) the filament collapse of the large filament spaced foams was significantly improved by the addition of the thermal field, which, in turn, improved the mechanical properties and manufacturing stability of the silicon foams. The heating-assisted direct writing process in this paper can facilitate the development of the field of microelectronics and the direct printing of biomaterials.

Predictive model of the solder paste stencil printing process by response surface methodology

PurposeThis paper aims to establish the predictive equations of height, area and volume of printed solder paste during solder paste stencil printing (SPSP) process in surface mount technology (SMT) to better understand the effect of process parameters on the printing quality.Design/methodology/approachAn experiment plan is proposed based on the response surface method (RSM). Experiments with 30 different combinations of process parameters are performed using a solder paste printer. After printing, the volume, area and height of the printed SAC105 solder paste are measured by a solder paste inspection machine. Using RSM, the predictive equations associated with the printing parameters and the printing quality of the solder paste are formed.FindingsThe optimal printing parameters are 175.08 N printing pressure, 250 mm/s printing speed, 0.1 mm snap-off height and 15.7 mm/s stencil snap-off speed if the target height of solder paste is 100 µm. As the target printing area of solder paste is 1.1 mm × 1.3 mm, the optimized values of the printing parameters are 140.29 N, 100.52 mm/s, 0.63 mm and 20.25 mm/s. When both the target printing height and area are optimized together, the optimal values for the four parameters are 86.67 N, 225.76 mm/s, 0.15 mm and 1.82 mm/s.Originality/valueA simple RSM-based experimental method is proposed to formulate the predictive polynomial equations for height, area and volume of printed solder paste in terms of important SPSP parameters. The predictive equation model can be applied to the actual SPSP process, allowing engineers to quickly predict the best printing parameters during parameter setting to improve production efficiency and quality.

Development of a Novel Reverse Offset Printer Equipped with Double-Layer Blanket (DLB) for Micropattern Printing on 3D Curved Surfaces

The double-layer blanket (DLB) reverse offset is a newly designed printing process for patterning electronic circuits on a 3D curved surface. Unlike the existing reverse offset process, the DLB reverse offset utilizes an offset roll composed of two layers comprising polydimethylsiloxane (PDMS) and a thick, soft cushioned rubber to print microelectrode patterns and transparent electrodes on a curved surface. The optimal printing process was determined by adjusting the printing pressure and printing speed for horizontal and vertical micropatterns, based on which transparent electrodes with metal mesh and honeycomb structures with a line width of 30 μm and pitch of 600 μm with micropatterns ranging from 30 μm to 60 μm were printed on a curved surface. Ag ink was used, and the 3D curved surface indicated a print quality similar to that of the flat surface for both the vertical and horizontal patterns and transparent electrodes. The DLB reverse offset technique demonstrated the possibility of printing on a 3D curved surface and is expected to broaden the range of printed electronics to applications such as smart glasses and 3D shape sensors.

3D Printing under High Ambient Pressures and Improvement of Mechanical Properties of Printed Parts

Contrary to other polymer processing methods, additive manufacturing processes do not require any pressure during the consolidation of layers. This study investigates the effect of high ambient pressure on the consolidation of layers during the FDM process and their analysis of mechanical properties. An experimental setup was arranged, consisting of a 3D printer integrated into a customized Autoclave, to achieve high strength properties for 3D printed parts as like injection-molded specimens. The autoclave can maintain 135 bar of pressure and a maximum temperature of 185 °C. 3D printing with PLA was carried out at 0 bar, 5 bar, and 10 bar. Tensile, flexural, and Charpy tests were conducted on printed specimens, and the effect of pressure and temperature on 3D-printed samples were analyzed. It could be shown that autoclave preheating before printing and autoclave pressure during printing improves the consolidation of layers immensely. The pressure inside the autoclave provokes a more intimate contact between the layer surfaces and results in higher mechanical properties such as yield strength, Young’s modulus, and impact strength. The properties could be raised 100%.

Highly Dispersed, Adhesive Carbon Nanotube Ink for Strain and Pressure Sensors.

Carbon nanotubes (CNT) with prominent electrical and mechanical properties are ideal candidates for flexible wearable devices. However, their poor dispersity in solvents greatly limits their applications as a conductive ink in the fabrication of wearable sensors. Herein, we demonstrate a kind of CNT-based conductive dispersion with high dispersity and adhesiveness using cellulose derivatives as the solvent, in which γ-aminopropyl triethoxy silane as a cross-linking agent reacts with cellulose to form copolymer networks, and simultaneously it also acts as an initiator to induce the self-polymerization of dopamine. Based on the conductive CNT ink, we also demonstrated textile-based strain sensors by stencil printing and sponge-based pressure sensors by the dipping method. The textile-based strain sensors could respond to external stimuli promptly. Then, the strain sensors were encapsulated via polydimethylsiloxane with the expansion of working ranges from less than 20 to nearly 70%. The encapsulated textile sensors exhibited excellent sensing performance as wearable strain sensors to monitor human motions including smile, throat vibration, finger folding, wrist bending, and elbow twisting. The sponge sensors hold high sensitivity and excellent durability as well. The conductive CNT-based ink provides an alternative idea in the development of flexible wearable devices.

Solvent‐Cast 3D Printing of Biodegradable Polymer Scaffolds

Abstract3D printing is a popular fabrication technique because of its ability to produce complex architectures. Melt‐based 3D printing is widely used for thermoplastic polymers like poly(caprolactone) (PCL), poly(lactic acid) (PLA), and poly(lactic‐co‐glycolic acid) (PLGA) because of their low processing temperatures. However, traditional melt‐based techniques require processing temperatures and pressures high enough to achieve continuous flow, limiting the type of polymer that can be printed. Solvent‐cast printing (SCP) offers an alternative approach to print a wider range of polymers. Polymers are dissolved in a volatile solvent that evaporates during deposition to produce a solid polymer filament. SCP, therefore, requires optimizing polymer concentration in the ink, print pressure, and print speed to achieve desired print fidelity. Here, capillary flow analysis shows how print pressure affects the process‐apparent viscosity of PCL, PLA, and PLGA inks. Ink viscosity is also measured using rheology, which is used to link a specific ink viscosity to a predicted set of print pressure and print speed for all three polymers. These results demonstrate how this approach can be used to accelerate optimization by significantly reducing the number of parameter combinations. This strategy can be applied to other polymers to expand the library of polymers printable with SCP.

3D printing of gelatin/chitosan biodegradable hybrid hydrogel: Critical issues due to the crosslinking reaction, degradation phenomena and process parameters

Hydrogel materials are being investigated for application as scaffolds in tissue engineering owing to their many advantages, such as high water content, softness and flexibility similar to many soft tissues, tuneable physical, chemical, and biological properties, excellent biocompatibility and biodegradability, and extensive framework for cell proliferation and survival. During the past decade, because of the great versatility offered in terms of processing approach, material selection, and customization, 3D printing has become a leading technology used to fabricate hydrogel scaffolds. Furthermore, high reproducibility and unparalleled control over structural and compositional characteristics make additive manufacturing the preferred technology for the fabrication of biodegradable hydrogel scaffolds. However, the production time could become critical in relation to any crosslinking reactions and degradation that may occur in the hydrogel and make the printing process unstable. In this study an analysis of the critical issues due to the crosslinking reaction and degradation phenomena have been executed following a statistical approach. In particular, three different experimental campaign demonstrate how the printing process became instable due to the mentioned phenomena. Finally, a procedure was developed to print gelatine-based biocompatible hydrogels with chitosan and functionalized polyethylene glycol as a cross linker (G-PEG-CH). In order to reach the printing temperature, the hydrogel mixture was initially stored in the refrigerator at 4°C for 12 h, followed by a 10 min incubation in warm water at 40°C. Based on this procedure, a filament strand of 950 μm with a standard deviation in the range of 20% was obtained by imposing a printing pressure of 1 bar and a printing speed of 100 mm/s at a temperature of 28 °C.

Predicting the extrudability of complex food materials during 3D printing based on image analysis and gray-box data-driven modelling

Material extrudability is a prerequisite for printing 3D structures with extrusion-based food printing. Food materials with different shear-thinning behaviors were printed to develop predictive models for extrudability. A dataset of 131 unique combinations of materials and printing parameters was collected. Image analysis was employed to rapidly quantify extrudability as determined by mode width, line height and width consistency of line filaments. The relation between the printing pressure and the volumetric flow rate followed a power-law relation, which characterizes the extent of shear-thinning of the food materials. Both regression and classification models were trained and tested using the random forest algorithm. The model performance indicated that extrudability can be predicted with moderate to high accuracy by using rheological measurements and printing parameters as inputs. The predictive workflow developed in this study provides a framework to quantitatively assess and predict extrudability for 3D printing of complex food materials. Industrial relevanceExtrusion-based 3D printing has been applied to customize food designs and can potentially enable personalized nutrition. To achieve this, we need to be able to effectively print complex food materials with high accuracy. Variations in composition and rheological properties of complex food materials make achieving proper extrudability not trivial at this moment, and this limits their applicability to extrusion-based food printing. Here, we developed an image analysis tool to measure line filament extrusion of complex food materials that vary in shear-thinning behaviors. We then built predictive models to estimate material extrudability based on material's shear-thinning properties and printing parameters. Data-driven prediction of material's extrudability can help avoid excessive trial-and error experiments in future.

Optimization of semisolid extrusion (pressure-assisted microsyringe)-based 3D printing process for advanced drug delivery application

3D printing of pharmaceutical formulations requires juggling between the material properties and the printing process to achieve the geometry of the 3D printed products with the desired dose and release. The purpose of the current investigation is to identify the material suitability for semisolid extrusion-based 3D printing of advanced drug delivery system. In this study, the semisolid extrusion-based 3D printing technique was optimized to print a controlled release shell as a vehicle/carrier for oral drug delivery system. The consistency of the printable paste was optimized by screening different solvents for the polymer of interest (cellulose acetate as optimum) to achieve the desired continuous and uniform extrudability behavior. The polarity of the solvent played important role in the selection of the suitable solvent for the optimization process. The nozzle shape (tapered tip as optimum) and the nozzle diameter (630 µm as optimum) were found to be also significantly influenced by extrusion behavior. The printing pressure (60 psi) and speed (4 mm/s) were optimized to reach the required rate of extrusion during the 3D printing process. The thickness and the width of the printed layers have contributed to the creation of the standard triangle language (STL) G-code for the printing of the 3D drug delivery system. It was found that the first printed layer deposited on a particular type of substrate plays a significant role to influence the spreadable behavior of extruded materials. Finally, the present study design proposes a rational guideline for the semisolid extrusion-based 3D printing of pharmaceutical products of specific interest for personalized medicine.

Contact printing pressure uniformization in roll-to-roll process using individual drive cross-coupled torque control

Contact printing pressure uniformization in roll-to-roll process using individual drive cross-coupled torque control

Optimization of flexographic print properties on ecologically favorable paper substrates

Nowadays, the attention in many industries is shifting towards the problem of waste overproduction and production of the waste in general. This study aimed to find an alternative approach to the production of packaging which will be environmentally friendly and at the same time optimal in terms of the print quality. This was accomplished by using the minimal needed amount of material for the production of packaging and adjusting the parameters of the flexographic printing process to achieve the desired visual impression of the print. The designed motive for the packaging was printed on five different recycled papers, following the guidelines of sustainable design. Printing was performed on each recycled paper with different printing pressures (50N, 150N, and 400N). Smoothness was measured on each paper; and for every printed sample, colorimetric measurements and thickness of the lines in positive and negative were measured. Print contrast for each print was calculated, and microscopy of fine printed elements was performed. All chosen papers except one had average smoothnes of up to 3.2 s (the smoothest paper had the average smootheness value of 54.72 s). Smoothness results influenced the printed line widths. Specifically, when increasing the printing pressure, a significant deformation of the line width has occured on all papers except the smoothest one (deformations of the lines printed in positive were up to 400 μm for rough papers compared to maximum of 60 μm for lines printed on the smoother paper). Similar results were obtained for the lines printed in negative. Furthermore, legibility of the printed typographic elements of 4pt size was significantly influenced by the smoothness of the paper. Elements printed on the smoothest paper have displayed the negligible deformations when changing the printing pressure. For other papers, elements in positive were optimally printed by 50 N pressure, and elements in negative by the pressure of 400 N. The results of this research have enabled the optimization of the flexographic printing process when using each of the five types of recycled papers. Furthermore, the presented qualitative and colorimetric parameters of the prints enabled the assessment of the applicability of used papers as printing substrates for ecologically favorable packaging.

Cell-Laden Nanocellulose/Chitosan-Based Bioinks for 3D Bioprinting and Enhanced Osteogenic Cell Differentiation.

3D bioprinting has recently emerged as a very useful tool in tissue engineering and regenerative medicine. However, developing suitable bioinks to fabricate specific tissue constructs remains a challenging task. Herein, we report on a nanocellulose/chitosan-based bioink, which is compatible with a 3D extrusion-based bioprinting technology, to design and engineer constructs for bone tissue engineering and regeneration applications. Bioinks were prepared using thermogelling chitosan, glycerophosphate, hydroxyethyl cellulose, and cellulose nanocrystals (CNCs). Formulations were optimized by varying the concentrations of glycerophosphate (80-300 mM), hydroxyethyl cellulose (0-0.5 mg/mL), and CNCs (0-2% w/v) to promote fast gelation kinetics (<7 s) at 37 °C and retain the shape integrity of constructs post 3D bioprinting. We investigated the effect of CNCs and pre-osteoblast cells (MC3T3-E1) on the rheological properties of bioinks, bioink printability, and mechanical properties of bioprinted scaffolds. We demonstrate that the addition of CNCs and cells (5 million cells/mL) significantly improved the viscosity of bioinks and the mechanical properties of chitosan scaffolds post-fabrication. The bioinks were biocompatible and printable at an optimized range of printing pressures (12-20 kPa) that did not compromise cell viability. The presence of CNCs promoted greater osteogenesis of MC3T3-E1 cells in chitosan scaffolds as shown by the upregulation of alkaline phosphatase activity, higher calcium mineralization, and extracellular matrix formation. The versatility of this CNCs-incorporated chitosan hydrogel makes it attractive as a bioink for 3D bioprinting to engineer scaffolds for bone tissue engineering and other therapeutic applications.

A Guided Evolutionary Search Approach for Real-Time Stencil Printing Optimization

This study aims to identify and maintain optimal stencil printer settings in dynamic surface mount technology (SMT) assembly lines to control the solder paste volume transfer efficiency (TE) and increase the yield rates of surface mount assembly (SMA) lines. Stencil printing process (SPP) is a crucial procedure that determines the first pass yields in SMA lines. Stencil printing speed (PS) and printing pressure (PP) are two critical SPP parameters in the manufacturing process; identifying the optimal printer settings in complex fabrication environment is cost efficient but challenging. To search for the global optimal PS and PP values in real time with minimal computational efforts, a dynamic optimization SPP optimization system is proposed based on a novel guided evolutionary search (GES) strategy. The GES algorithm is flexible to adjust the feasible solution region based on real-time optimization results to maximize the probability of finding optimal solutions and minimize the computational burden. As a major extension on the classical evolutionary search, the GES minimizes the possibility to fall in the local optimal due to insufficiencies in the prediction accuracy and guarantees global optimality within the dynamically varying printing environment.

Three-dimensional biofabrication of an aragonite-enriched self-hardening bone graft substitute and assessment of its osteogenicity in vitro and in vivo.

ABSTRACTA self-hardening three-dimensional (3D)-porous composite bone graft consisting of 65 wt% hydroxyapatite (HA) and 35 wt% aragonite was fabricated using a 3D-Bioplotter®. New tetracalcium phosphate and dicalcium phosphate anhydrous/aragonite/gelatine paste formulae were developed to overcome the phase separation of the liquid and solid components. The mechanical properties, porosity, height and width stability of the end products were optimised through a systematic analysis of the fabrication processing parameters including printing pressure, printing speed and distance between strands. The resulting 3D-printed bone graft was confirmed to be a mixture of HA and aragonite by X-ray diffraction, Fourier transform infrared spectroscopy and energy dispersive X-ray spectroscopy. The compression strength of HA/aragonite was between 0.56 and 2.49 MPa. Cytotoxicity was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in vitro. The osteogenicity of HA/aragonite was evaluated in vitro by alkaline phosphatase assay using human umbilical cord matrix mesenchymal stem cells, and in vivo by juxtapositional implantation between the tibia and the anterior tibialis muscle in rats. The results showed that the scaffold was not toxic and supported osteogenic differentiation in vitro. HA/aragonite stimulated new bone formation that bridged host bone and intramuscular implants in vivo. We conclude that HA/aragonite is a biodegradable and conductive bone formation biomaterial that stimulates bone regeneration. Since this material is formed near 37°C, it will have great potential for incorporating bioactive molecules to suit personalised application; however, further study of its biodegradation and osteogenic capacity is warranted. The study was approved by the Animal Ethical Committee at Tongji Medical School, Huazhong University of Science and Technology (IACUC No. 738) on October 1, 2017.

Characteristics of Tobacco Stem Particleboard in Printing Load Variations and Storage Time

Tobacco is an important commodity in the economy in Indonesia. Tobacco stems are worthless waste. Tobacco stems contain 56.10% cellulose, making it high potential as a particle board raw material. The process of making particleboard that affects quality is the pressing process during printing. Greater pressure printing presses make bonds between particles stronger. Strong bonding between particles is intended so that the board does not change during storage. The purpose of this study is to determine the characteristics of particleboard in various variations of printing pressure load and storage time and to find out the best treatment as a recommendation in the manufacture of tobacco stem particleboard. The research method was factorial CRD with a combination of treatments between 2 factors namely printing load (4 kg and 8 kg) and storage time (0, 12, and 24 days). The results showed 1.13 - 1.25 g.cm-3 for the density value, water content 4.97 - 12.57%, and water absorption 240.03 - 208.00%. MOE and MOR values ​​were 16000.13 - 36222.77 kgf. cm-2 and 824.47 - 1697.83 kgf.cm-2. The recommended treatment load is 8 kg printing pressure with 12 days of storage time.

Bioprinting and Preliminary Testing of Highly Reproducible Novel Bioink for Potential Skin Regeneration.

Three-dimensional (3D) bioprinting is considered as a novel approach in biofabricating cell-laden constructs that could potentially be used to promote skin regeneration following injury. In this study, a novel crosslinked chitosan (CH)–genipin (GE) bioink laden with keratinocyte and human dermal fibroblast cells was developed and printed successfully using an extruder-based bioprinter. By altering the composition and degree of CH–GE crosslinking, bioink printability was further assessed and compared with a commercial bioink. Rheological analysis showed that the viscosity of the optimised bioink was in a suitable range that facilitated reproducible and reliable printing by applying low pressures ranging from 20–40 kPa. The application of low printing pressures proved vital for viability of cells loaded within the bioinks. Further characterisation using MTT assay showed that cells were still viable within the printed construct at 93% despite the crosslinking, processing and after subjecting to physiological conditions for seven days. The morphological study of the printed cells showed that they were mobile within the bioink. Furthermore, the multi-layered 3D printed constructs demonstrated excellent self-supportive structures in a consistent manner.

Naked-Eye 3D Display Based on Microlens Array Using Combined Micro-Nano Imprint and UV Offset Printing Methods.

An optical film integrating microlens array (MLAs) and 3D micro-graphics is an important way to achieve the naked-eye 3D display effect. The 3D micro-graphics is traditionally generated by the micro-nano imprint technology based on precision engraving mold, which leads to high production cost and low production efficiency, and thus restricts the rapid response to production tasks and large-scale popularization and application. In this study, a process scheme for large-scale printing of 3D micro-graphics using UV offset printing based on presensitized (PS) plate was proposed, matching with the MLAs fabricated by micro-nano imprint process to achieve naked-eye 3D display effect. We used the laser confocal microscope to systematically measure and analyze the geometric and optical performance of the fabricated MLAs in terms of height, curvature radius, center distance, spacing, focal length, and numerical aperture, and evaluated the influence of the publishing resolution of the PS plate on the display effect of 3D micro-graphics. The printing quality and display effect of 3D micro-graphics were further improved by adjusting process parameters such as printing speed and printing pressure. The results of the current study demonstrate that the combined application of micro-nano imprint technology based on precision mold and UV offset printing technology based on PS plate can achieve an excellent naked-eye 3D display effect in 360° all angles, which is efficient, cost-saving, and highly flexible.