The purpose of this study is to explore whether there is N400 effect on the representation of gender stereotype in the different picture priming condition from the behavioral and ERP levels, and further explore whether there is hierarchical structure of upper category, secondary category, typical example and counter-example based on this. The results showed: (1) under the condition of picture priming, N400 effect would be induced when representing the conflict of gender stereotypes. (2) Category representation and example representation can activate different regions of the brain. When the priming stimulus was upper category (gender picture) and secondary category (occupational gender picture), N400 effect mainly appeared on the electrode of frontal region in left hemisphere.When the priming stimuli were typical example (typical example picture) and counter-example, the N400 effect mainly appeared on the electrodes in the frontal region of the right hemisphere.(3) the gender stereotype representation of picture activation has hierarchical structure, that is, N400 amplitude induced by upper category activation < secondary category activation < typical sample activation < counter-example activation. These findings suggest that the representation of gender stereotypes has a hierarchical structure at the picture level.