Primary Amide Group Research Articles

Discovery of novel benzimidazole derivatives as selective and reversible monoamine oxidase B inhibitors for Parkinson's disease treatment

Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the loss of dopaminergic neurons in the substantia nigra pars compacta. The development of novel scaffolds for human monoamine oxidase B (hMAO-B) inhibitors with reversible properties represents an important strategy to improve the efficacy and safety for PD treatment. In the current work, we have devised and assessed two innovative derivative series serving as hMAO-B inhibitors. These series have utilized benzimidazole as a scaffold and strategically incorporated a primary amide group, which is recognized as a pivotal pharmacophore in subsequent activity screening and reversible mode of action. Among these compounds, 16d has emerged as the most potent hMAO-B inhibitor with an IC50 value of 67.3 nM, comparable to safinamide (IC50 = 42.6 nM) in vitro. Besides, 16d demonstrated good selectivity towards hMAO-B isoenzyme with a selectivity index over 387. Importantly, in line with the design purpose, 16d inhibited hMAO-B in a competitive and reversible manner (Ki = 82.50 nM). Moreover, 16d exhibited a good safety profile in both cellular and acute toxicity assays in mice. It also displayed ideal pharmacokinetic properties and blood-brain barrier permeability in vivo, essential prerequisites for central nervous system medicines. In the MPTP-induced PD mouse model, 16d significantly alleviated the motor impairment, especially muscle relaxation and motor coordination. Therefore, 16d, serving as a lead compound, holds instructive significance for subsequent investigations regarding its application in the treatment of PD.

Vermiculture-based molybdenum oxide nanoparticles synthesis, optimization, characterization and its impact on seed germination and seedling characteristics in green gram (Vigna radiata)

Here, we report the biosynthesis of molybdenum oxide (Mo5O14) nanoparticles (NPs) by co-precipitation methods using various vermiculture-based extracts and their impact on seed germination in Vigna radiata. The synthesis of Mo5O14 NPs was initially characterized by a color change from yellow to white and a surface plasmon resonance (SPR) peak at 360 nm in UV–visible spectroscopic analysis. In addition, X-ray diffraction (XRD) data revealed that the Mo5O14 NPs were crystalline with a tetragonal structure and a size of 35.32–41.86 nm. Furthermore, fourier transform infrared (FTIR) analysis of NPs absorption bands revealed the presence of unique functional groups, such as -OH stretching, CH2 stretching, and primary amide groups, providing strong evidence that the native protein served to a reduce, cap, and stabilize Mo5O14 NPs. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images revealed that the ultrastructural profile of Mo5O14 NPs formed rod particles with an average particle length of 102 nm and width of 33 nm. The energy dispersive X-ray (EDX) pattern analysis confirmed the presence of Mo and O ions. Finally, the effects of Mo5O14 NPs on Vigna radiata seed fresh and dry biomass characteristics, root and shoot length, and seed germination were investigated, and it was found that a significant increase in the seed germination rate of all seven vermiculture-based Mo5O14 NPs was observed at 200 ppm. Consequently, our work provides a step forward in the development of vermiculture-based Mo5O14 NPs as a promising green agrochemical nano-fertilizer.

Facile Synthesis of Catechol-Containing Polyacrylamide Copolymers: Synergistic Effects of Amine, Amide and Catechol Residues in Mussel-Inspired Adhesives.

The straightforward synthesis of polyamide-derived statistical copolymers with catechol, amine, amide and hydroxy residues via free radical polymerization is presented. In particular, catechol, amine and amide residues are present in natural mussel foot proteins, enabling strong underwater adhesion due to synergistic effects where cationic residues displace hydration and ion layers, followed by strong short-rang hydrogen bonding between the catechol or primary amides and SiO2 surfaces. The present study is aimed at investigating whether such synergistic effects also exist for statistical copolymer systems that lack the sequence-defined positioning of functional groups in mussel foot proteins. A series of copolymers is established and the adsorption in saline solutions on SiO2 is determined by quartz crystal microbalance measurements and ellipsometry. These studies confirm a synergy between cationic amine groups with catechol units and primary amide groups via an increased adsorptivity and increased polymer layer thicknesses. Therefore, the free radical polymerization of catechol, amine and amide monomers as shown here may lead to simplified mussel-inspired adhesives that can be prepared with the readily scalable methods required for large-scale applications.

Intramolecular cyclization of 15-carboxy-13-amide derivatives of chlorin e6 under the action of 2-Chloro-1-methylpyridinium iodide

We have shown the possibility of intramolecular cyclization with the participation of the carboxyl group at position 15 of the chlorin macrocycle with a primary or secondary amide group at position 13 through the formation of an imide bond under the action of 2-Chloro-1-methylpyridinium iodide; the imide bond can be used to form a seven-membered exocycle. Using this reaction, we synthesized a number of cycloimide derivatives of chlorophyll a. The proposed synthetic route can be carried out if the substituent at the amide nitrogen atom has a hydroxyl, dimethylaminomethyl or vinyl group, which opens up possibilities for further chemical modification of the obtained derivatives.

Reductive N-alkylation of primary amides using nickel-nanoparticles

Here we report Ni-nanoparticles as reusable catalysts for reductive N-alkylation of amides. These Ni-nanoparticles based catalysts have been prepared by the template synthesis of tartaric acid and 2-methyl imidazole ligated Ni-complex on SiO2 and subsequent pyrolysis under argon. Applying optimal Ni-nanostructured catalyst, N-alkylation of aromatic and heterocyclic primary amides with different aldehydes in presence of molecular hydrogen was performed to access structurally diverse N-alkylated amides in good to excellent yields. In addition, the applicability of this N-alkylation protocol has been demonstrated for the selective functionalization of primary amide group in Levetiracetam drug.

Survival and growth of saprotrophic and mycorrhizal fungi in recalcitrant amine, amide and ammonium containing media.

The elimination of hazardous compounds in chemical wastes can be a complex and technically demanding task. In the search for environmental-friendly technologies, fungal mediated remediation and removal procedures are of concern. In this study, we investigated whether there are fungal species that can survive and grow on solely amine-containing compounds. One compound containing a primary amine group; 2-diethylaminoethanol, one compound with a primary amide group; 2,6-dichlorobenzamide (BAM), and a third compound containing a quaternary ammonium group; N3-trimethyl(2-oxiranyl)methanaminium chloride, were selected. The choice of these compounds was motivated by their excessive use in large scale manufacturing of protein separation media (2-diethylaminoethanol and the quaternary amine). 2,6-dichlorobenzamide, the degradation product of the herbicide 2,6-dichlorobenzonitrile (dichlobenil), was chosen since it is an extremely recalcitrant compound. Utilising part of the large fungal diversity in Northern European forests, a screening study using 48 fungal isolates from 42 fungal species, including saprotrophic and mycorrhizal fungi, was performed to test for growth responses to the chosen compounds. The ericoid (ERM) mycorrhizal fungus Rhizoscyphus ericae showed the best overall growth on 2-diethylaminoethanol and BAM in the 1-20 g L-1 concentration range, with a 35-fold and 4.5-fold increase in biomass, respectively. For N3-trimethyl(2-oxiranyl)methanaminium chloride, the peak growth occurred at 1 g L-1. In a second experiment, including three of the most promising fungi (Laccaria laccata, Hygrophorus camarophyllus and Rhizoscyphus ericae) from the screening experiment, a simulated process water containing 1.9% (w/v) 2-diethylaminoethanol and 0.8% (w/v) N3-trimethyl(2-oxiranyl)methanaminium chloride was used. Laccaria laccata showed the best biomass increase (380%) relative to a control, while the accumulation for Rhizoscyphus ericae and Hygrophorus camarophyllus were 292% and 136% respectively, indicating that mycorrhizal fungi can use amine- and amide-containing substrates as nutrients. These results show the potential of certain fungal species to be used in alternative green wastewater treatment procedures.

Charge-assisted bond and molecular self-assembly drive the gelation of lenvatinib mesylate

Lenvatinib mesylate (LM) is a first-line anticancer agent for the treatment of unresectable hepatocellular carcinoma, while it formed viscoelastic hydrogel when contacting with aqueous medium, which would significantly hinder its in vitro dissolution. The aim of this study was to systematicly explore the gelation mechanism and gel properties via thermal analysis, rheology, morphology and spectroscopy studies. The formed hydrogel was found to be composed of a new polymorph of crystalline LM, and its mechanical strength depended on the cross-linking degree of the fibrillar network structure. Spectroscopy analyses revealed that the intermolecular hydrogen bonds (the bifurcated hydrogen bond between the adjacent urea groups and the NH⋯OC hydrogen bond between the primary amide groups) as well as π-π stacking interactions (between the benzene ring and the quinoline ring) were suggested to be the driving forces for the self-assembly of LM during gelation process. Additionally, no gelation phenomenon was observed when suspending the base form lenvatinib in water, while it could form gel in various acidic solutions (e.g. hydrochloric acid, phosphoric acid and methanesulfonic acid) because the regenerated N+-H group increased the solubility of lenvatinib and promoted the balance between the dissolution or aggregation of LX (X: acid radical ion) molecules in solutions. In conclusion, the charge-assisted bond N+-H in LM molecule and intermolecular non-covalent interactions drived the hydrogel formation of LM in aqueous media. This study elucidates the gelation mechanism and gel properties of LM hydrogel, which would be helpful to figure out strategy to eliminate its gelation fundamentally and pave the way for its further formulation development in future.

Ru(II)-Catalyzed Controlled Cross-Dehydrogenative Coupling of Benzamides with Activated Olefins via Weakly Coordinating Primary Amides.

Ru(II)-catalyzed regioselective ortho-alkenylation of primary benzamides with activated olefins has been realized over the competitive cyclized products. This reaction overall proceeds via a cross-dehydrogenative coupling (CDC) reaction using a simple and weakly coordinating primary amide group in the presence of an inexpensive Ru(II) salt and allows the controlled introduction of olefin motifs at the ortho-position of benzamides. The key to the success of this strategy depends on fine-tuning the reaction conditions. The developed protocol has demonstrated excellent regio/diastereoselectivity and a good functional group tolerance with wide substrate scope and obviates the requirement of external auxiliaries as well as the costly metal catalyst. Detailed mechanistic studies indicate the involvement of the base-assisted internal electrophilic-type substitution (BIES) step in the reaction mechanism.

A combined experimental and computational structural study of the N-(2-cyanophenyl)disulfonamides derived from 5-bromo- and 5-iodoanthranilamide

One-pot sulfonyl chloride-pyridine mediated disulfonylation of the aniline nitrogen atom and dehydration of the primary amide group of the 5-halogenoanthranilamide derivatives afforded the corresponding N-(2-cyanophenyl)disulfonamides, exclusively. This facile reaction makes use of readily available substrates and gives high yield of the products in short reaction time. The structures of these compounds were characterised using a combination of nuclear magnetic resonance (1H NMR & 13C NMR), infrared (IR) and mass spectrometric techniques. Their geometry was distinctly confirmed by single crystal X-ray diffraction (XRD) analysis of N-(4-bromo-2-cyanophenyl)-N-(methylsulfonyl)methanesulfonamide (2a) as representative model. The geometrical optimizations of structures of compounds 2a, 2b and 2c were also investigated by means of density functional theory (DFT) calculations. The optimized structural parameters (bond lengths, bond angles, and torsion angles) of 2a have been compared with those of the X-ray structure.

Amino Acid-Containing Phase-Selective Organogelators: A Water-Based Delivery System for Oil Spill Treatment.

The simple structural modification of replacing a terminal carboxylic acid with a primary amide group was found to lower the minimum gelation concentration (MGC), by at least an order of magnitude, for a series of N-lauroyl-l-amino acid phase-selective organogelators in decane. The amide-functionalized analogue N-lauroyl-l-alanine-CONH2 was demonstrated to gel a broad range of solvents from diesel to THF at MGCs of 2.5% w/v or less, as well as to produce gels with a higher thermal stability (ca. 30 °C) and enhanced mechanical properties (5 times increase in complex modulus), compared to the carboxylic acid analogue, N-lauroyl-l-alanine-COOH. These improved properties may be due to the additional hydrogen bonding in the primary amide analogue as revealed by SCXRD. Most significantly for this study, the introduction of the primary amide functionality enabled N-lauroyl-l-alanine-CONH2 to form a self-assembled fibrillar network in water. The aqueous network could then actively uptake and rapidly gel decane, diesel, and diluted bitumen (“dilbit”) with MGCs of 2.5% w/v or less. This aqueous delivery method is advantageous for oil-remediation applications as no harmful carrier solvents are required and the gel can be easily separated from the water, allowing the oil to be recovered and the gelator recycled.

Mechanistic Insights of the Critical Role of Hydrogen Donor in Controlling Drug Release From Acrylate Adhesive

In the present study, a pyrrolidone adhesive and an amide adhesive were synthesized, and their molecular mechanisms of controlled drug release were described. Using zolmitriptan as model drug, in vitro drug release and skin permeation experiments were performed. Adhesive properties were evaluated using modulated differential scanning calorimetry and rheology study. Free volume of polymer was directly obtained by positron annihilation lifetime spectroscopy. Intermolecular interactions between drugs and adhesives were determined by FTIR spectroscopic analysis and molecular simulation. Release percent (24 h) of zolmitriptan from pyrrolidone adhesive was about 55.8 ± 3.1% (w/w), while from amide adhesive, the release percent (24 h) was about 40.1 ± 1.6% (w/w). The free volume sizes of pyrrolidone adhesive and amide adhesive were about 2309.6 Å3 and 2854.5 Å3, respectively, which were much larger than molecular volume of zolmitriptan (about 285.7 Å3). Thus, the polymer networks might not hinder drug diffusion from the view of free volume. Comparing chemical structures of pyrrolidone group and primary amide group, the main difference was that primary amide group of amide adhesive possessed 2 hydrogen donors. It was proved that hydrogen bonding between zolmitriptan and hydrogen donor of primary amide group played a critical role in controlling drug release.

Design and Development of a Heterogeneous Catalyst for the Michael Addition of Malononitrile to 2-Enoylpyridines: Influence of the Primary Amide Decorated Framework on Catalytic Activity and Selectivity.

For the Michael addition of malononitrile to 2-enoylpyridines, we report the first heterogeneous catalyst, {[Zn2(2-bpbg)(fum)2]·4H2O·EtOH}n (1) (where 2-bpbg = N,N'-bis(2-pyridylmethyl)-1,4-diaminobutane-N,N'-diacetamide and where fum = fumarate), which is decorated with primary amide side arms. It is prepared from the self-assembly of starting materials in methanol at room temperature (27 °C). Using 3 mol % of 1, greater than 99% conversion of substrates to the desired product is achieved within 1 h at 27 °C. Moreover, the catalyst is recyclable up to five consecutive cycles without significant loss of activity and structural integrity. In order to show the uniqueness of 1, the reaction under the same conditions was catalyzed by a fully pyridyl-based (i.e., having no primary amide group) and isostructural analogue, {[Zn2(tpbn)(fum)2]·6H2O}n (2) (where tpbn = N',N',N″,N''-tetrakis(pyridin-2-ylmethyl)butane-1,4-diamine), but resulting only in 7% conversion. This demonstrates the selective catalytic activity of 1 over 2 due to the presence of the primary amide side arms, where it acts as a bifunctional catalyst through the excellent hydrogen bond donating (HBD) ability in this reaction. For providing an insight into its mechanism of action involving a cyclic seven-membered hydrogen bonding motif, the reaction was performed with freshly synthesized (E)-chalcone, 3-enoylpyridine, and 4-enoylpyridine instead of 2-enoylpyridine under the same conditions. In the case of (E)-chalcone no product formation was observed, whereas for 3-enoylpyridine and 4-enoylpyridine the conversions were only 29% and 25%, respectively. Both 1 and 2 were fully characterized by infrared spectroscopy, elemental analysis, thermogravimetric analysis, and single-crystal and powder X-ray diffraction.

Surface Modification of Polypropylene with Primary Amines by Acrylamide Radiation Grafting and Hofmann's Transposition Reaction

AbstractPolypropylene (PP) film surface is modified with primary amines by means of gamma radiation to graft acrylamide (AAm) using the pre‐irradiation oxidative method at a dose of 50 kGy, a monomer concentration of 13%, and a temperature of 60 °C for 6 h, followed by Hofmann rearrangement to reduce the primary amide groups of the grafted polymer (PP‐g‐AAm) to obtain vinylamine (VAm) groups (PP‐g‐VAm). The films are characterized using infrared spectroscopy before and after the reduction reaction; the hydrophilicity is studied using the contact angle; the thermal stability of the films are observed using TGA and DSC; and the surface morphology is determined using SEM. The presence of primary amines and the corresponding decrease in C=O and O=C‐NH2 groups due to reduction reaction, are confirmed using X‐ray photoelectron spectroscopy. In addition, the surface primary amines are quantified using a colorimetric method that calculated the amount as 1.9+0.1 nmol/mm2.

Engineering a Nanoscale Primary Amide-Functionalized 2D Coordination Polymer as an Efficient and Recyclable Heterogeneous Catalyst for the Knoevenagel Condensation Reaction

This work reports the design, structural characterization, and catalytic behavior of the first example of primary amide-functionalized coordination polymers (CPs), namely, {[Cd2(2-bpbg)(fum)2(H2O)2]·8.5H2O}n (1) (where 2-bpbg = N,N′-bis(2-pyridylmethyl)-1,4-diaminobutane-N,N′-diacetamide and fum = fumarate). CP 1 is synthesized from a one-pot self-assembly of starting materials in methanol under ambient conditions in excellent yield and purity, allowing an easy access to multigram quantities of it within few hours. As an example, CP 1 was used as a highly efficient heterogeneous catalyst in the carbon–carbon bond-forming Knoevenagel condensation reaction for the conversion of benzaldehyde to benzylidene malononitrile. CP 1 possesses both Lewis acidic and Bronsted basic character for the presence of unsaturated metal sites and primary amide groups, respectively, making it a highly competent bifunctional catalyst for such type of reactions. Surprisingly, on the one hand, 100% conversion was observed using o...

Main-Group-Catalyzed Reductive Alkylation of Multiply Substituted Amines with Aldehydes Using H2.

Given the growing demand for green and sustainable chemical processes, the catalytic reductive alkylation of amines with main-group catalysts of low toxicity and molecular hydrogen as the reductant would be an ideal method to functionalize amines. However, such a process remains challenging. Herein, a novel reductive alkylation system using H2 is presented, which proceeds via a tandem reaction that involves the B(2,6-Cl2C6H3)( p-HC6F4)2-catalyzed formation of an imine and the subsequent hydrogenation of this imine catalyzed by a frustrated Lewis pair (FLP). This reductive alkylation reaction generates H2O as the sole byproduct and directly functionalizes amines that bear a remarkably wide range of substituents including carboxyl, hydroxyl, additional amino, primary amide, and primary sulfonamide groups. The synthesis of isoindolinones and aminophthalic anhydrides has also been achieved by a one-pot process that consists of a combination of the present reductive alkylation with an intramolecular amidation and intramolecular dehydration reactions, respectively. The reaction showed a zeroth-order and a first-order dependence on the concentration of an imine intermediate and B(2,6-Cl2C6H3)( p-HC6F4)2, respectively. In addition, the reaction progress was significantly affected by the concentration of H2. These results suggest a possible mechanism in which the heterolysis of H2 is facilitated by the FLP comprising THF and B(2,6-Cl2C6H3)( p-HC6F4)2.

Efficient preparation of β-hydroxy aspartic acid and its derivatives

We report an efficient and practical synthetic route to various properly-protected erythreo-β-OH-Asp compounds, which are key β-branched α-amino acid units in coralmycin A and other peptide natural products. Fmoc and cyclic ketal-protected erythreo-β-OH-Asp 7 is prepared from cheap chiral precursor l-diethyl tartrate in six steps without the need of column purification. The modified form of 7 serves as a versatile precursor to various β-alkoxyl analogs of erythreo-β-OH-Asp. In addition, we successfully performed a model study toward the total synthesis of coralmycin A, featuring a late stage installation of the side chain primary amide group of erythreo-β-OMe-Asn.

Nonadditive Interactions Mediated by Water at Chemically Heterogeneous Surfaces: Nonionic Polar Groups and Hydrophobic Interactions.

We explore how two nonionic polar groups (primary amine and primary amide) influence hydrophobic interactions of neighboring nonpolar domains. We designed stable β-peptide sequences that generated globally amphiphilic (GA) helices, each with a nonpolar domain formed by six cyclohexyl side chains arranged along one side of the 14-helix. The other side of the helix was dominated by three polar side chains, from β3-homolysine (K) and/or β3-homoglutamine (Q) residues. Variations in this polar side chain array included exclusively β3-hLys (GA-KKK) and β3-hLys/β3-hGln mixtures (e.g., GA-QKK and GA-QQK). Chemical force measurements in aqueous solution versus methanol allowed quantification of the hydrophobic interactions of the β-peptide with the nonpolar tip of an atomic force microscope (AFM). At pH 10.5, where the K side chain is largely uncharged, we measured hydrophobic adhesive interactions mediated by GA-KKK to be 0.61 ± 0.04 nN, by GA-QKK to be 0.54 ± 0.01 nN, and by GA-QQK to be 0 ± 0.01 nN. This finding suggests that replacing an amine group (K side chain) with a primary amide group (Q side chain) weakens the hydrophobic interaction generated by the six cyclohexyl side chains. AFM studies with solid-supported mixed monolayers containing an alkyl component (60%) and a component bearing either a terminal amide or an amine group (40%) revealed analogous trends. These observations from two distinct experiment systems indicate that proximal nonionic polar groups have pronounced effects on hydrophobic interactions generated by a neighboring nonpolar domain, and that the magnitude of the effect depends strongly on polar group identity.

Enzymatic C-H Oxidation-Amidation Cascade in the Production of Natural and Unnatural Thiotetronate Antibiotics with Potentiated Bioactivity.

The selective activation of unreactive hydrocarbons by biosynthetic enzymes has inspired new synthetic methods in C-H bond activation. Herein, we report the unprecedented two-step biosynthetic conversion of thiotetromycin to thiotetroamide C involving the tandem oxidation and amidation of an unreactive ethyl group. We detail the genetic and biochemical basis for the terminal amidation in thiotetroamide C biosynthesis, which involves a uniquely adapted cytochrome P450-amidotransferase enzyme pair and highlights the first oxidation-amidation enzymatic cascade reaction leading to the selective formation of a primary amide group from a chemically inert alkyl group. Motivated by the ten-fold increase in antibiotic potency of thiotetroamide C ascribed to the acetamide group and the unusual enzymology involved, we enzymatically interrogated diverse thiolactomycin analogues and prepared an unnatural thiotetroamide C analogue with potentiated bioactivity compared to the parent molecule.

Enzymatic C−H Oxidation–Amidation Cascade in the Production of Natural and Unnatural Thiotetronate Antibiotics with Potentiated Bioactivity

AbstractThe selective activation of unreactive hydrocarbons by biosynthetic enzymes has inspired new synthetic methods in C−H bond activation. Herein, we report the unprecedented two‐step biosynthetic conversion of thiotetromycin to thiotetroamide C involving the tandem oxidation and amidation of an unreactive ethyl group. We detail the genetic and biochemical basis for the terminal amidation in thiotetroamide C biosynthesis, which involves a uniquely adapted cytochrome P450–amidotransferase enzyme pair and highlights the first oxidation–amidation enzymatic cascade reaction leading to the selective formation of a primary amide group from a chemically inert alkyl group. Motivated by the ten‐fold increase in antibiotic potency of thiotetroamide C ascribed to the acetamide group and the unusual enzymology involved, we enzymatically interrogated diverse thiolactomycin analogues and prepared an unnatural thiotetroamide C analogue with potentiated bioactivity compared to the parent molecule.

Biomimetic synthesis and anticancer activity ofEurycoma longifoliabranch extract‐mediated silver nanoparticles

In the present study, silver nanoparticles (AgNPs) were synthesised by adding 1 mM Ag nitrate solution to different concentrations (1%, 2.5%, 5%) of branch extracts of Eurycoma longifolia, a well known medicinal plant in South–East Asian countries. Characterisation of AgNPs was carried out using techniques such as ultraviolet–visible spectrophotometry, X-ray diffractrometry, Fourier transform infrared–attenuated total reflection spectroscopy (FTIR–ATR), scanning electron microscopy. XRD analysis revealed face centre cubic structure of AgNPs and FTIR–ATR showed that primary and secondary amide groups in combination with the protein molecules present in the branch extract were responsible for the reduction and stabilisation of AgNPs. Furthermore, antioxidant [2,2-diphenyl-1-picrylhydrazyl and 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulphonic acid)], antimicrobial and anticancer activities of AgNPs were investigated. The highest bactericidal activity of these biogenic AgNPs was found against Escherichia coli with zone inhibition of 11 mm. AgNPs exhibited significant anticancer activity against human glioma cells (DBTRG and U87) and human breast adenocarcinoma cells (MCF-7 and MDA-MB-231) with IC50 values of 33, 42, 60 and 38 µg/ml.