Previous Analyses Research Articles

Direct measurement of the inertial-convective subrange above ocean surface waves

The −5/3 power law over the inertial subrange of the turbulence kinetic energy spectrum is one of the most well-known concepts in fluid physics. Obukhov and Corrsin extended the original hypothesis to a passive tracer, leading to the concept of an inertial-convective subrange. These postulates have been empirically validated in the atmospheric turbulence over land but have not been comprehensively studied in the marine air flow. During a recent oceanic campaign, the platform FLIP was deployed with an array of sensors to measure the perturbation wind velocity, temperature, and water vapor. Using these data, a previous study found that Kolmogorov's hypothesized −5/3 was not universally valid over the ocean. Here, we continue that work to analyze the spectrum of temperature and water vapor to empirically evaluate the theoretical extension by Obukhov-Corrsin. For temperature, the observed spectra were too noisy for thorough analysis; our conjecture for the source of noise and its implications for near-surface observations of atmospheric turbulence are discussed. For water vapor, we found strong agreement with the previous analysis of the kinetic energy spectrum. These findings corroborate (1) the theoretical notion of the scalar energy dissipation subrange driven by the inertial motions in the marine boundary layer and (2) evidence for non-Kolmogorov turbulence in the high Reynolds flow immediately above ocean waves. Our analysis shows a strong relationship with distance from the wavy surface; using linear extrapolation, we find that divergence from −5/3 persists in the lowest 25 m of the atmosphere.

A Deep uGMRT View of the Ultra-steep Spectrum Radio Halo in A521

Abstract We present the first detailed analysis of the ultra-steep spectrum radio halo in the merging galaxy cluster A521, based on upgraded Giant Metrewave Radio telescope observations. The combination of radio observations (300–850 MHz) and archival X-ray data provides a new window into the complex physics occurring in this system. When compared to all previous analyses, our sensitive radio images detected the centrally located radio halo emission to a greater extent of ∼1.3 Mpc. A faint extension of the southeastern radio relic has been discovered. We detected another relic, recently discovered by MeerKAT, and coincident with a possible shock front in the X-rays, at the northwest position of the center. We find that the integrated spectrum of the radio halo is well-fitted with a spectral index of −1.86 ± 0.12. A spatially resolved spectral index map revealed the spectral index fluctuations, as well as an outward radial steepening of the average spectral index. The radio and X-ray surface brightness is well correlated for the entire and different subparts of the halo, with sublinear correlation slopes (0.50–0.65). We also found a mild anticorrelation between the spectral index and X-ray surface brightness. Newly detected extensions of the SE relic and the counter relic are consistent with the merger in the plane of the sky.

Potential Structural Biomarkers in 3D Images Validated by the First Functional Biomarker for Early Age-Related Macular Degeneration - ALSTAR2 Baseline.

Lack of valid end points impedes developing therapeutic strategies for early age-related macular degeneration (AMD). Delayed rod-mediated dark adaptation (RMDA) is the first functional biomarker for incident early AMD. The relationship between RMDA and the status of outer retinal bands on optical coherence tomography (OCT) have not been well defined. This study aims to characterize these relationships in early and intermediate AMD. Baseline data from 476 participants was assessed including eyes with early AMD (n=138), intermediate AMD (n=101), and normal aging (n=237). Participants underwent volume OCT imaging of the macula and rod intercept time (RIT) was measured. The ellipsoid zone (EZ) and interdigitation zone (IZ) on all OCT B-scans of the volumes were segmented. The area of detectable EZ and IZ, and mean thickness of IZ within the Early Treatment Diabetic Retinopathy Study (ETDRS) grid were computed and associations with RIT were assessed by Spearman's correlation coefficient and age adjusted. Delayed RMDA (longer RIT) was most strongly associated with less preserved IZ area (r=-0.591; P<0.001), followed by decreased IZ thickness (r=-0.434; P<0.001), and EZ area (r=-0.334; P<0.001). This correlation between RIT and IZ integrity was not apparent when considering normal eyes alone within 1.5 mm of the fovea. RMDA is correlated with the status of outer retinal bands in early and intermediate AMD eyes, particularly, the status of the IZ. This correlation is consistent with a previous analysis of only foveal B-scans and is biologically plausible given that retinoid availability, involving transfer at the interface attributed to the IZ, is rate-limiting for RMDA.

Two of a Kind: Comparing Big and Small Black Holes in Binaries with Gravitational Waves

When modeling the population of merging binary black holes, analyses have generally focused on characterizing the distribution of primary (i.e., more massive) black holes in the binary, while using simplistic prescriptions for the distribution of secondary masses. However, the secondary mass distribution and its relationship to the primary mass distribution provide a fundamental observational constraint on the formation history of coalescing binary black holes. If both black holes experience similar stellar evolutionary processes prior to collapse, as might be expected in dynamical formation channels, the primary and secondary mass distributions would show similar features. If they follow distinct evolutionary pathways (for example, due to binary interactions that break symmetry between the initially more massive and less massive stars), their mass distributions may differ. We present the first analysis of the binary black hole population that explicitly fits for the secondary mass distribution. We find that the data is consistent with a ∼30 M ⊙ peak existing only in the distribution of secondary rather than primary masses. This would have major implications for our understanding of the formation of these binaries. Alternatively, the data is consistent with the peak existing in both component mass distributions, a possibility not included in most previous studies. In either case, the peak is observed at 31.4−2.6+2.3M⊙ , which is shifted lower than the value obtained in previous analyses of the marginal primary mass distribution, placing this feature in further tension with expectations from a pulsational pair-instability supernova pileup.

The Causal Association Between Medication Intake and Increased Risk of Psoriasis.

Psoriasis is a chronic, inflammatory, and papulo-squamous skin disorder without a radical cure. Although previous observational analyses have discovered a relationship between medication intake and increased risk of psoriasis, they are susceptible to confounders. We intend to ascertain if there is a causal association between specific medication intake and increased risk of psoriasis by utilizing the Mendelian randomization (MR) method. We obtained the genome-wide association study (GWAS) data for medication intake (23 types, N = 1809) from UK Biobank samples. And we sourced the GWAS data for psoriasis from the 8th release of the FinnGen database, which included 8,075 psoriasis cases and 330,975 healthy control cases. Then a two-sample MR study was performed to determine their causal association, and inverse-variance-weighted MR (IVW-MR) was applied to calculate the effect estimates. The IVW-MR analysis uncovered a positive correlation between the intake of HMG CoA reductase inhibitors and the increased risk of psoriasis (odds ratio [OR] = 1.167, 95% confidence interval [CI] = 1.084-1.257). Similarly, the use of thyroid preparations (OR=1.080, 95% CI=1.026-1.138), nonsteroidal anti-inflammatory and antirheumatic products (OR=1.406, 95% CI=1.037-1.908), anilides (OR=1.379, 95% CI=1.004-1.894), antihistamines for systemic use (OR=1.341, 95% CI=1.104-1.630), and antihypertensives (OR=1.099, 95% CI=1.016-1.190) were associated with an increased risk of psoriasis. We did not find evidence from IVW-MR for other associations. Our study offers a causal testimony that the intake of HMG CoA reductase inhibitors, thyroid preparations, nonsteroidal anti-inflammatory and antirheumatic products, anilides, antihistamines for systemic use, and antihypertensives will potentially increase the risk of psoriasis.

Comparative genomics of Japanese encephalitis virus shows low rates of recombination and a small subset of codon positions under episodic diversifying selection.

Orthoflavivirus japonicum (JEV) is the dominant cause of viral encephalitis in the Asian region with 100,000 cases and 25,000 deaths reported annually. The genome is comprised of a single polyprotein that encodes three structural and seven non-structural proteins. We collated a dataset of 349 complete genomes from a number of public databases, and analysed the data for recombination, evolutionary selection and phylogenetic structure. There are low rates of recombination in JEV, subsequently recombination is not a major evolutionary force shaping JEV. We found a strong overall signal of purifying selection in the genome, which is the main force affecting the evolutionary dynamics in JEV. There are also a small number of genomic sites under episodic diversifying selection, especially in the envelope protein and non-structural proteins 3 and 5. Overall, these results support previous analyses of JEV evolutionary genomics and provide additional insight into the evolutionary processes shaping the distribution and adaptation of this important pathogenic arbovirus.

Nuclear phylogenomics of angiosperms and insights into their relationships and evolution.

Angiosperms (flowering plants) are by far the most diverse land plant group with over 300,000 species. The sudden appearance of diverse angiosperms in the fossil record was referred to by Darwin as the "abominable mystery," hence contributing to the heightened interest in angiosperm evolution. Angiosperms display wide ranges of morphological, physiological, and ecological characters, some of which have probably influenced their species richness. The evolutionary analyses of these characteristics help to address questions of angiosperm diversification and require well resolved phylogeny. Following the great successes of phylogenetic analyses using plastid sequences, dozens to thousands of nuclear genes from next-generation sequencing have been used in angiosperm phylogenomic analyses, providing well resolved phylogenies and new insights into the evolution of angiosperms. In this review we focus on recent nuclear phylogenomic analyses of large angiosperm clades, orders, families, and subdivisions of some families and provide a summarized Nuclear Phylogenetic Tree of Angiosperm Families. The newly established nuclear phylogenetic relationships are highlighted and compared with previous phylogenetic results. The sequenced genomes of Amborella, Nymphaea, Chloranthus, Ceratophyllum, and species of monocots, Magnoliids, and basal eudicots, have facilitated the phylogenomics of relationships among five major angiosperms clades. All but one of the 64 angiosperm orders were included in nuclear phylogenomics with well resolved relationships except the placements of several orders. Most families have been included with robust and highly supported placements, especially for relationships within several large and important orders and families. Additionally, we examine the divergence time estimation and biogeographic analyses of angiosperm on the basis of the nuclear phylogenomic frameworks and discuss the differences compared with previous analyses. Furthermore, we discuss the implications of nuclear phylogenomic analyses on ancestral reconstruction of morphological, physiological, and ecological characters of angiosperm groups, limitations of current nuclear phylogenomic studies, and the taxa that require future attention.

Analysis of International Disputes over Digital Services Tax and Exploration of China’s Approach

With the continuous emergence of emerging information technologies, the digital economy is developing rapidly around the world. The global operations of multinational digital enterprises no longer rely on physical presence. The effectiveness of permanent establishment rules based on the traditional real economy settings has been questioned by market countries, which actively participate in Market countries where digital business value is created do not receive corresponding taxation rights. Multinational digital companies take advantage of loopholes in digital platforms and current tax rules to actively carry out tax planning, resulting in digital companies’ global tax burden being much lower than that of traditional companies. Tax sources cannot be fairly and reasonably distributed in the places where value is created and profits are taxed, which harms the tax interests of market countries. Against the background of slow progress in the OECD global unified solution, as a temporary unilateral measure to address the challenges of the digital economy, some countries have taken the lead in imposing digital service taxes on specific digital businesses of digital enterprises. On the basis of discussing the relevant concepts and theories of digital service tax, this article discusses the relevant concepts and theories of digital service tax, and analyzes the international development of digital service tax and the positions of various countries around the world. Further analyze the impact of digital service tax collection from three aspects: national fiscal revenue, tax trade disputes and international tax order coordination, and analyze the impact of digital service tax on digital enterprises. Then the perspective is focused on my country, sorting out and summarizing my country's relevant digital economy tax policies, and analyzing the impact of digital service tax on my country in the near term and long term based on the development of my country's digital economy. Finally, based on the previous comparative analysis and impact analysis, my country's tax system for promoting the development of the digital economy has gradually improved and actively participated in the reshaping of international tax rules, and my country's response strategies under the digital service tax have been proposed. The different levels of digital economic development between China and countries that have levied digital service taxes will inevitably lead to disharmony in the international tax order. The study found that the fiscal revenue brought by digital service tax is limited and has a certain degree of distortion effect. It is necessary to actively participate in international cooperation and make China's voice heard before reaching a consensus on international tax rules. In the long term, we should focus on the connection between the two-pillar plan and the domestic tax system, strengthen tax information exchange, improve the domestic digital tax rule system, and promote the healthy and sustainable development of my country's digital economy.

Somatic genome architecture and molecular evolution are decoupled in "young" linage-specific gene families in ciliates.

The evolution of lineage-specific gene families remains poorly studied across the eukaryotic tree of life, with most analyses focusing on the recent evolution of de novo genes in model species. Here we explore the origins of lineage-specific genes in ciliates, a ~1 billion year old clade of microeukaryotes that are defined by their division of somatic and germline functions into distinct nuclei. Previous analyses on conserved gene families have shown the effect of ciliates' unusual genome architecture on gene family evolution: extensive genome processing-the generation of thousands of gene-sized somatic chromosomes from canonical germline chromosomes-is associated with larger and more diverse gene families. To further study the relationship between ciliate genome architecture and gene family evolution, we analyzed lineage specific gene families from a set of 46 transcriptomes and 12 genomes representing x species from eight ciliate classes. We assess how the evolution lineage-specific gene families occurs among four groups of ciliates: extensive fragmenters with gene-size somatic chromosomes, non-extensive fragmenters with "large'' multi-gene somatic chromosomes, Heterotrichea with highly polyploid somatic genomes and Karyorelictea with 'paradiploid' somatic genomes. Our analyses demonstrate that: 1) most lineage-specific gene families are found at shallow taxonomic scales; 2) extensive genome processing (i.e., gene unscrambling) during development likely influences the size and number of young lineage-specific gene families; and 3) the influence of somatic genome architecture on molecular evolution is increasingly apparent in older gene families. Altogether, these data highlight the influences of genome architecture on the evolution of lineage-specific gene families in eukaryotes.

Exploiting Machine Learning and Disequilibrium in Galaxy Clusters to Obtain a Mass Profile

We use 3D k-means clustering to characterize galaxy substructure in the A2146 cluster of galaxies (z = 0.2343). This method objectively characterizes the cluster’s substructure using projected position and velocity data for 67 galaxies within a 2.305 Mpc circular region centered on the cluster's optical center. The optimal number of substructures is found to be four. Four distinct substructures with rms velocity typical of galaxy groups or low-mass subclusters, when compared to cosmological simulations of galaxy cluster formation, suggest that A2146 is in the early stages of formation. We utilize this disequilibrium, which is so prevalent in galaxy clusters at all redshifts, to construct a radial mass distribution. Substructures are bound but not virialized. This method is in contrast to previous kinematical analyses, which have assumed virialization, and ignored the ubiquitous clumping of galaxies. The best-fitting radial mass profile is much less centrally concentrated than the well-known Navarro–Frenk–White profile, indicating that the dark-matter-dominated mass distribution is flatter pre-equilibrium, becoming more centrally peaked in equilibrium through the merging of the substructure.

Modeling and Comparing the Total Cost of Ownership of Passenger Automobiles with Conventional, Electric, and Hybrid Powertrains

&lt;div&gt;The global automotive industry’s shift toward electrification hinges on battery electric vehicles (BEV) having a reduced total cost of ownership compared to traditional vehicles. Although BEVs exhibit lower operational costs than internal combustion engine (ICE) vehicles, their initial acquisition expense is higher due to expensive battery packs. This study evaluates total ownership costs for four vehicle types: traditional ICE-based car, BEV, split-power hybrid, and plug-in hybrid. Unlike previous analyses comparing production vehicles, this study employs a hypothetical sedan with different powertrains for a more equitable assessment. The study uses a drive-cycle model grounded in fundamental vehicle dynamics to determine the fuel and electricity consumption for each vehicle in highway and urban conditions. These figures serve a Monte Carlo simulation, projecting a vehicle’s operating cost over a decade based on average daily distance and highway driving percentage. Results show plug-in hybrids generally offer the most economical choice. Due to the BEVs’ heavier weight and battery cost, they only become more cost-effective than plug-in hybrids after 160 km daily travel, associated with only a small percentage of drivers in the United States. Nevertheless, they remain cheaper than conventional vehicles for most distances. The study also investigates the effects of government subsidies, battery cost, and weight on overall expenses for each powertrain. It concludes that opting for less expensive, albeit heavier batteries would generally reduce EV ownership costs for consumers.&lt;/div&gt;

DOP16 Safety of long-term ozanimod treatment for up to 4 years in patients with moderately to severely active Ulcerative Colitis: an interim analysis of the True North open-label extension

Abstract Background The 52-week, phase 3 True North (TN) study (NCT02435992) demonstrated the efficacy and safety of ozanimod (OZA), a highly selective sphingosine 1-phosphate receptor 1 and 5 modulator, in patients (pts) with moderately to severely active ulcerative colitis (UC). A prior analysis reported the long-term safety of up to 3 y of OZA in the ongoing TN open-label extension (OLE; NCT02531126; from TN baseline to OLE Week [W] 94). This analysis presents the cumulative long-term safety of OZA in TN and the TN OLE for an additional year (up to OLE W142). Methods The TN study design has been previously described. This analysis included all TN pts who entered the OLE from TN (clinical nonresponders at W10, lost response during maintenance, or completed maintenance at W52). Safety assessments were reported from the first dose of OZA either in TN or OLE up to OLE W142. Lab abnormalities, including absolute lymphocyte count (ALC) reductions, were reported. Exposure-adjusted incidence rates (EAIRs) per 100 patient-years (PY) were calculated for all treatment-emergent adverse events (TEAEs). Results A total of 823 pts entered the TN OLE (average age: 41.7 y; 59% male); 62% had left-sided UC disease. Total PY exposure to OZA at OLE W142 was 2536 y. Overall, the cumulative safety assessments did not significantly change with an additional year of OZA exposure from OLE W94 to W142 (Table). A decrease in EAIRs per 100 PY was observed in overall TEAEs (85.5 vs 87.6) and serious TEAEs (7.0 vs 7.4) at OLE W142 vs OLE W94, respectively. Notably, with an additional year of OZA exposure, TEAEs leading to treatment discontinuation remained at 2.5 EAIR/100 PY, and there were no new cases of bradycardia or atrioventricular block. One new TEAE each of herpes zoster, macular oedema, cystoid macular oedema, myocardial ischaemia, pulmonary embolism, and deep vein thrombosis were noted. COVID-19 TEAEs increased at OLE W142 (n=103; EAIR: 4.3/100 PY) from OLE W94 (n=66; EAIR: 3.0/100 PY). Reductions in ALC &amp;lt;500 cells/mm3 were common in the OLE (OLE W94, 54.0%; OLE W142, 56.1%); few pts had ALC &amp;lt;200 cells/mm3 (OLE W94, 5.3%; OLE W142, 6.5%), but these pts had no serious infections. No serious hepatic safety events were observed. One new death due to adenocarcinoma, which was unrelated to OZA, was reported. Conclusion The ongoing safety profile of OZA is consistent with previous analyses, with no new safety signals identified compared with previous UC analyses. Long-term use of OZA representing 2536 PYs of exposure continues to be well tolerated in pts with moderately to severely active UC.

P349 Communicating Needs and Features of IBD Experiences (CONFIDE) Survey: Descriptive Comparison of Experiences of Bowel Urgency among Patients with Ulcerative Colitis in Canada, Europe, and the US

Abstract Background Bowel urgency is a disruptive symptom affecting daily lives of patients with moderate-to-severe ulcerative colitis (UC). Previous analyses from the Communicating Needs and Features of IBD Experiences (CONFIDE) survey reported bowel urgency as a common and impactful symptom of UC in the United States (US) and Europe. This study used data from the CONFIDE survey to descriptively compare the prevalence and impact of bowel urgency in patients with UC across US, Europe, and Canada. Methods Online, quantitative, cross-sectional surveys were conducted among patients with UC in the US, Europe (France, Germany, Italy, Spain, UK), Japan, and Canada. Criteria based on previous treatment, steroid use, and/or hospitalization defined moderate-to-severe UC. Descriptive comparisons of data from US, Europe, and Canada are presented. Results Surveys were completed by 82 Canadian, 556 European, and 200 US patients with UC. Across geographies, most patients were receiving advanced therapies at the time of survey completion (Canada:73%, Europe:54%, US: 77%; Table). Similar to the findings in Europe and US, bowel urgency was among the top three most common symptoms currently (in the last month) experienced by Canadian patients (Canada:31%, Europe:30%, US:47%; Figure 1a). Due to fear/anticipation of bowel urgency-related accidents, 60% Canadian patients reported wearing diaper/pad/other protection, at least once a month in the past 3 months, similar to European (65%) and US (69%) patients. Among Canadian patients who ever experienced bowel urgency (n=46, 56%), most (78%) reported doing so at least once a month in past 3 months with similar frequencies observed among US and European patients; proportion of patients experiencing bowel urgency at least once a week was higher among US (79%) and European (71%) patients than Canadian patients (48%) (Table). Among patients receiving advanced therapies, similar patterns were observed (Table). Most patients in all three populations reported bowel urgency and bowel urgency-related accidents among the top five most common reasons for declining participation in work/school, social events, and sports/physical exercise (Figure 1b). Conclusion These descriptive analyses suggest that although the overall prevalence and burden of bowel urgency in Canada are similar to those in the US and Europe, a numerically lower proportion of Canadian patients with moderate-to-severe UC experienced bowel urgency at least once a week in past 3 months. Despite receiving advanced therapies, patients experience bowel urgency and related diaper/protection use across geographies. These findings highlight the clinical importance of evaluating and treating bowel urgency to improve the quality of life of patients with UC across geographies.

P956 Pregnancy outcomes after maternal or paternal exposure in the tofacitinib ulcerative colitis clinical programme

Abstract Background Pregnant women with ulcerative colitis (UC) have a higher risk of adverse pregnancy outcomes vs age-matched controls.1 Tofacitinib is an oral Janus kinase inhibitor for the treatment of UC. There are no well-controlled studies on tofacitinib use in pregnant women. It was shown to be teratogenic in rats and rabbits at approximately 73 and 6.3 times, respectively, the maximum recommended human dose of 10 mg twice daily. Tofacitinib affects female fertility, parturition and peri/postnatal development in rats and was secreted into the milk of lactating rats; it had no effects on male fertility or sperm motility/concentration. Methods We report pregnancy outcomes in the tofacitinib UC clinical programme: 4 randomised, placebo-controlled Phase (P)2/3 studies (NCT00787202/NCT01465763/NCT01458951/NCT01458574),2,3 an open-label, long-term extension study (NCT01470612)4 and a randomised P3b/4 study (NCT03281304) were analysed.5 Study protocols excluded pregnant women, required females of childbearing potential to use effective contraception and regularly tested for urine β-hCG. Study drug was discontinued in female patients who became pregnant. Pregnancy outcomes after maternal or paternal tofacitinib exposure were identified from Pfizer’s internal safety database up to March 2023 and categorised as healthy newborn, medical termination, foetal death, congenital malformation, spontaneous abortion or lost to follow-up. Results There were 40 pregnancies with exposure to tofacitinib: 16 cases of maternal exposure (median age 29.5 [range: 24−41] years), all during the 1st trimester (10 [62.5%] healthy newborns, 2 [12.5%] medical terminations, 2 [12.5%] spontaneous abortions, 2 [12.5%] lost to followup); and 24 cases of paternal exposure (18 [75.0%] healthy newborns, 2 [8.3%] spontaneous abortions, 4 [16.7%] lost to follow-up). There were no cases of foetal death or congenital malformation. Conclusion Most known outcomes for maternal and paternal tofacitinib exposure were healthy newborns, similar to previous analyses in other tofacitinib clinical study populations and the general UC population.6,7 Limitations included the low number of reported pregnancies and available follow-up. Tofacitinib should not be used during pregnancy unless necessary.

Non-Equilibrium Wigner Function and Application to Model of Catalyzed Polymerization.

The quantum Wigner function and non-equilibrium equation for a microscopic particle in one spatial dimension (1D) subject to a potential and a heat bath at thermal equilibrium are considered by non-trivially extending a previous analysis. The non-equilibrium equation yields a general hierarchy for suitable non-equilibrium moments. A new non-trivial solution of the hierarchy combining the continued fractions and infinite series thereof is obtained and analyzed. In a short thermal wavelength regime (keeping quantum features adequate for chemical reactions), the hierarchy is approximated by a three-term one. For long times, in turn, the three-term hierarchy is replaced by a Smoluchovski equation. By extending that 1D analysis, a new model of the growth (polymerization) of a molecular chain (template or te) by binding an individual unit (an atom) and activation by a catalyst is developed in three spatial dimensions (3D). The atom, te, and catalyst move randomly as solutions in a fluid at rest in thermal equilibrium. Classical statistical mechanics describe the te and catalyst approximately. Atoms and bindings are treated quantum-mechanically. A mixed non-equilibrium quantum-classical Wigner-Liouville function and dynamical equations for the atom and for the te and catalyst, respectively, are employed. By integrating over the degrees of freedom of te and with the catalyst assumed to be near equilibrium, an approximate Smoluchowski equation is obtained for the unit. The mean first passage time (MFPT) for the atom to become bound to the te, facilitated by the catalyst, is considered. The resulting MFPT is consistent with the Arrhenius formula for rate constants in chemical reactions.

The mediating effect of hope agency on perceived stress and professional burnout among Polish corporate employees

Job burnout is considered an outcome of prolonged exposure of employees to stress. Although many studies have focused on the presence of a direct association between stress and burnout, we still know very little about mediators that indirectly play a role in this relationship. Previous analyses have determined that self-efficacy acts as a mechanism that explains the overall relationship between stress and burnout. However, there is no such evidence to support the mediatory function of hope. Therefore, the main aim of the current study was to verify whether self-efficacy, hope pathways, and hope agency are mediators in this relationship. The study included 408 Polish-speaking adults who completed the Perceived Stress Scale, the Maslach Burnout Inventory, the Generalized Self-Efficacy Scale, and the Dispositional Hope Scale. The outcomes indicated a positive correlation of stress with the overall burnout score, as well as all subscales. Moreover, hope agency was a mediator, thus suggesting that there is also an indirect relationship between stress and job burnout. Therefore, it can be assumed that higher stress is associated with lower motivation to generate and sustain the actions needed to reach the goals. Consequently, lower hope agency may lead stressed employees to greater exhaustion and reduced personal accomplishment.

Genetic diversity and recent ancestry based on whole-genome sequencing of endangered Swedish cattle breeds.

Several indigenous cattle breeds in Sweden are endangered. Conservation of their genetic diversity and genomic characterization is a priority.Whole-genome sequences (WGS) with a mean coverage of 25X, ranging from 14 to 41X were obtained for 30 individuals of the breeds Fjällko, Fjällnära, Bohuskulla, Rödkulla, Ringamåla, and Väneko. WGS-based genotyping revealed 22,548,028 variants in total, comprising 18,876,115 single nucleotide polymorphisms (SNPs) and 3,671,913 indels. Out of these, 1,154,779 SNPs and 304,467 indels were novel. Population stratification based on roughly 19 million SNPs showed two major groups of the breeds that correspond to northern and southern breeds. Overall, a higher genetic diversity was observed in the southern breeds compared to the northern breeds. While the population stratification was consistent with previous genome-wide SNP array-based analyses, the genealogy of the individuals inferred from WGS based estimates turned out to be more complex than expected from previous SNP-array based estimates. Polymorphisms and their predicted phenotypic consequences were associated with differences in the coat color phenotypes between the northern and southern breeds. Notably, these high-consequence polymorphisms were not represented in SNP arrays, which are used routinely for genotyping of cattle breeds.This study is the first WGS-based population genetic analysis of Swedish native cattle breeds. The genetic diversity of native breeds was found to be high. High-consequence polymorphisms were linked with desirable phenotypes using whole-genome genotyping, which highlights the pressing need for intensifying WGS-based characterization of the native breeds.

Fast Sampling via Spectral Independence Beyond Bounded-degree Graphs

Spectral independence is a recently developed framework for obtaining sharp bounds on the convergence time of the classical Glauber dynamics. This new framework has yielded optimal O(n log n) sampling algorithms on bounded-degree graphs for a large class of problems throughout the so-called uniqueness regime, including, for example, the problems of sampling independent sets, matchings, and Ising-model configurations. Our main contribution is to relax the bounded-degree assumption that has so far been important in establishing and applying spectral independence. Previous methods for avoiding degree bounds rely on using L p -norms to analyse contraction on graphs with bounded connective constant (Sinclair, Srivastava, and Yin, FOCS’13). The non-linearity of L p -norms is an obstacle to applying these results to bound spectral independence. Our solution is to capture the L p -analysis recursively by amortising over the subtrees of the recurrence used to analyse contraction. Our method generalises previous analyses that applied only to bounded-degree graphs. As a main application of our techniques, we consider the random graph G (n, d/n) , where the previously known algorithms run in time n O (log d ) or applied only to large d . We refine these algorithmic bounds significantly, and develop fast nearly linear algorithms based on Glauber dynamics that apply to all constant d , throughout the uniqueness regime.

Stakeholder analysis for effective implementation of water management system: Case of groundwater charge in South Korea

Groundwater Charge was introduced in 2005 as one of the sustainable resource management measures in South Korea. The implementation rate, however, stagnated around 37 %, indicating that most local governments chose not to adopt this ‘optional’ regulation. While previous Stakeholder Analysis studies mainly blamed exclusion – or limited involvement – of stakeholders in the designing and structuring stage of policy-making process for policy failures, this study focused on the interest conflicts and dynamics hindered implementation process. This is because the issue with the subject policy, i.e., Groundwater Charge in South Korea, is low ‘implementation rate’ not the ‘collection rate’ or ‘tax deficit.’ If it was simply design or structural issue, the Charge should suffer from tax deficit problem due to lower tax income than operational costs. Thus, in order to investigate the reasons of low Charge adoption rate at the local government level, the Stakeholder Analysis Theory was applied to examine each stakeholder of the Charge to distinguish the interaction among supportive and opposing groups. The analysis revealed that there are only strong opponents of the policy without clearly identifiable supporters. Having agricultural & fishery industry and small independent businesses in spas, hotels, and swimming pool as strong Players, the Context setters (local governments) are not motivated to enforce Groundwater charge. Furthermore, today's social norm governed by economic efficiency is preventing the environmentalists and other Subjects to counteract Players. Under these circumstances, this study recommends the Subject to transform the Crowds (general public) into policy supporters through education. Environmental education is the only viable means to encourage necessary paradigm shift to enable effective implementation of environmental policies like Groundwater charge.

The serine protease 2 gene regulates lipid metabolism through the LEP/ampkα1/SREBP1 pathway in bovine mammary epithelial cells

Molecular breeding has brought about significant transformations in the milk market and production system during the twenty-first century. The primary economic characteristic of dairy production pertains to milk fat content. Our previous transcriptome analyses revealed that serine protease 2 (PRSS2) is a candidate gene that could impact milk fat synthesis in bovine mammary epithelial cells (BMECs) of Chinese Holstein dairy cows. To elucidate the function of the PRSS2 gene in milk fat synthesis, we constructed vectors for PRSS2 overexpression and interference and assessed intracellular triglycerides (TGs), cholesterol (CHOL), and nonesterified fatty acid (NEFA) contents in BMECs. Fatty acid varieties and components were also quantified using gas chromatography‒mass spectrometry (GC‒MS) technology. The regulatory pathway mediated by PRSS2 was validated through qPCR, ELISA, and WB techniques. Based on our research findings, PRSS2 emerges as a pivotal gene that regulates the expression of associated genes, thereby making a substantial contribution to lipid metabolism via the leptin (LEP)/Adenylate-activated protein kinase, alpha 1 catalytic subunit (AMPKα1)/sterol regulatory element binding protein 1(SREBP1) pathway by inhibiting TGs and CHOL accumulation while potentially promoting NEFA synthesis in BMECs. Furthermore, the PRSS2 gene enhances intracellular medium- and long-chain fatty acid metabolism by modulating genes related to the LEP/AMPKα1/SREBP1 pathway, leading to increased contents of unsaturated fatty acids C17:1N7 and C22:4N6. This study provides a robust theoretical framework for further investigation into the underlying molecular mechanisms through which PRSS2 influences lipid metabolism in dairy cows.