Prevalence Of Mites Research Articles

In vitro culture and morphological observation of human eye demodex mites

The prevalence of human Demodex mites has surged in recent years, prompting significant concern among both patients and the medical community. This study aimed to investigate the survival duration and morphological alterations of Demodex folliculorum under diverse temperature conditions and in various culture media. We employed the eyelash sampling technique to procure the mites. The collected specimens were then subjected to culture at two distinct temperature ranges (16–22 °C and 4 °C) across a spectrum of media, including 30% tea tree oil (TTO), phosphate-buffered saline (PBS), pure water, 0.9% physiological saline, 5 µg/ml propidium iodide (PI), liquid paraffin, glycerol, and a blank culture medium. Post-administration, the mites’ activity and morphological changes were meticulously documented. Our findings indicate that the survival span of Demodex mites within the same medium was notably extended at 4 °C compared to room temperature. Specifically, under 4 °C, the use of liquid paraffin as a culture medium yielded the longest survival time of 12 days, surpassing all other conditions. Remarkably, the morphological integrity of the mites in this group remained largely unaltered. These results suggest that 4 °C is the optimal temperature for the in vitro cultivation of Demodex mites, offering insights into the environmental preferences of these organisms and potentially informing future therapeutic strategies.

Epidemiological survey of two morphotypes of Demodex folliculorum (Prostigmata: Demodicidade) in young people from southern Spain

Different morphotypes of Demodex from humans have been described. Among them, molecular studies have made it possible to discern between the Demodex folliculorum and Demodex brevis. Further studies showed two morphotypes of D. folliculorum harboured two different habits (human skin and human eyelashes), both of them with finger-shaped terminal opisthosoma difficult to differentiate and that can be assigned to D. folliculorum,Thus, a complete morphometric study of the species, which in this study are referred to as Demodex folliculorum species complex, was carried out. From this morphometric and meristic study two distinct morphological forms (short and long) could be identified within the Demodex folliculorum species complex. These forms differ significantly in four out of the six biometric parameters we analysed: gnathosomal length and width, podosomal width, and opisthosomal length. Moreover, a comprehensive survey of the two morphotypes from different habitats (skin and eyelashes), was carried out in young people of Southern Spain. Therefore, an analysis of 104 asymptomatic students, which were contact lens wearers, and the presence of D. folliculorum was carried out. A statistical analysis based on Bayesian zero inflated Poisson GLM has been applied to our sample data. For the age group considered (18–24 years old), the overall D. folliculorum prevalence for skin face or eye infections (at least one of them) was 19.31 % (51 people), with a statistically significant higher prevalence in males men. Furthermore, there is a slight statistical correlation between the presence of Demodex in silicone hydrogel soft contact lens wearers. This study confirms: i) the existence of two morphotypes of D. folliculorum that appear segregated due to the parasitization microhabitats,ii) a higher prevalence of mites in men than in women, iii) the existence of a high number of statistically supported double infections (skin-eyelashes). It also provides epidemiological data on the prevalence of long and short forms of D. folliculorum in a healthy young population.

Varroa destructor mite Prevalence in Beekeeping Farms in Lankaran-Astara Economic Region of Azerbaijan

Beekeeping improvements and attention to this subject are considerable in Azerbaijan. It is not correct to indicate only the climate factor as the cause of low productivity and bee losses in honey bees. The presence of a number of pests in bees leads to a decrease in their ability to work, as well as to make them more susceptible to diseases and quickly infected. Among such pests, we can mention the Varroa destructor mite. In our research, the prevalence of the Varroa mite, which is a pest of honey bees, was studied in Jalilabad, Masallli, Yardimli, Lankaran, Lerik and Astara regions of Azerbaijan’s Lankaran-Astara economic region. According to the results, the region with the highest infection rate was Astara and Lankaran, 80%, and the region with the lowest infection rate was 50% in Masalli district.

Nasal mites in Molothrus ater (Aves: Icteridae) from different geographic locations in the USA

ABSTRACT Nasal mites are endoparasites that spend their entire life cycle inside the nasal cavities and respiratory passages of birds. The brown-headed cowbird, Molothrus ater (Boddaert, 1783) (hereafter BHCO), is an icterid bird that uses brood parasitism as a reproductive strategy in which it lays eggs in the nests of other bird species and allows the host to raise its young. Interestingly, nasal mites previously collected from brown-headed cowbirds represent some species shared by other icterids and others known from common host taxa. In this study, we examined how nasal mite species richness and prevalence differed geographically across three of the four migratory flyways of North America in a large sample size of BHCO. In total, ~4000 nasal mites (~3900 Rhinonyssidae (Mesostigmata) and 85 Ereynetidae (Trombidiformes)), representing nine species, were recovered from 856 cowbirds with a prevalence of 89%. Nasal mite prevalence was not significantly related with geographic location or host sex, suggesting that patterns of infection in BHCO occur similarly in all locations.

Varroa destructor economic injury levels and pathogens associated with colony losses in Western Canada

IntroductionThe honey bee parasitic mite, Varroa destructor, is considered the main factor associated with winter colony losses. To control this mite, beekeepers must implement Integrated Pest Management (IPM) strategies. An effective IPM strategy is based on timely monitoring of mite levels to treat the colonies when the mites reach an economic threshold and before they grow to damaging levels (economic injury level). Additionally, concerns have been raised about the possible interaction between V. destructor and other stressors; stressors acting additively or synergistically may lead to high colony mortality.MethodologyVarroa destructor levels were recorded in colonies from five regions of British Columbia, Canada: Lower Mainland, Kootenay, Okanagan, Cariboo, and Peace. Mite levels were analyzed for associations with colony strength and mortality, as well as with other pathogens that were identified using total RNA sequencing.ResultsThe Kootenay and Lower Mainland regions had the highest V. destructor levels in the fall (3.38±0.65 and 2.51±0.57, respectively) followed by the Okanagan (1.54±0.36), Cariboo (1.31±0.39), and Peace (0.66±0.25) regions. The Peace region had a significantly lower proportion of dead colonies than the other regions. Varroa destructor levels in the fall significantly predicted odds of colony mortality in the spring. Colonies with ≥ 1% mite infestation in the fall (August) had a significantly higher mortality rate (p=0.005) compared to colonies with <1% mite levels. Therefore, treatment is recommended above that threshold. Also, deformed wing virus-B levels were 8.4 times higher than those of deformed wing virus-A variant in all the regions and were highest in the Kootenay region, which also had the highest rate of colony losses. Lastly, Malpighamoeba mellificae and Nosema (Vairimorpha) apis transcripts were identified along with other pathogens that deserve further investigation, including Apis mellifera filamentous virus, Apis rhabdovirus, Lake Sinai virus, Varroa tymo-like, and Apis mellifera solinvivirus 1.DiscussionThe dynamic nature of host-pathogen interactions requires frequent pathogen surveillance to determine the prevalence and levels of parasitic mites and associated infectious diseases, and their impact on honey bee health, not only in the regions of this study, but globally. Nevertheless, more studies are needed to determine the nature of the type of interaction between the identified pathogens and V. destructor. Also, more research is needed to evaluate their potential impact on colony survival, to provide accurate mite and disease control recommendations to beekeepers.

Explorational analysis of the abundance and prevalence of chigger and gamasid mites parasitic on small mammals in Vietnam.

We studied chigger and gamasid mite loads on small mammals during the dry season in Vietnam and used both our field data and museum collections to estimate the influence of environmental factors on mite abundance and prevalence. Generalized linear (mixed effect) models were used to analyze the data. We examined 1,239 small mammal individuals, which were obtained from field expeditions and museum collections belonging to 59 species. In different localities, Rattus Fischer (Rodentia: Muridae), Niviventer Marshall (Rodentia: Muridae), and Maxomys Sody (Rodentia: Muridae) were the most common animals captured. The prevalence of chigger and gamasid mites in our expedition data was high: 72% and 62%, respectively. We found differences in the abundance of chigger mites between different populations of the same species of small mammals. Season and locality were the main factors that influenced chigger mite abundance and prevalence. The best model that predicted the abundance and prevalence of chigger mites included geography (province) as a predictor and host species and season as random effects. For the first time, we analyzed factors connected with climate and weather affecting chigger mites of small mammals in Vietnam.

Human Demodex mites (Acari: Demodicidae) as a possible etiological factor in Rosacea: A cross-sectional study from Turkey

This study was conducted to determine the prevalence and burden of Demodex mites in patients with rosacea, and to investigate their potential contribution to the aetiology of rosacea. The study included 82 patients (44 with papulopustular rosacea and 38 with erythematotelangiectatic rosacea) diagnosed with rosacea by clinical examination and 82 healthy controls without dermatologic disease. Samples were obtained from each participant from the cheek, nasolabial, and chin regions using standardised skin surface biopsy (SSSB). Samples were examined under a light microscope, and Demodex species were identified and counted. Demodex positivity (≥5 mites) was detected in 91% of patients and 20% of controls in the study (p< 0.01). Only D. folliculorum (total 1328, mean 44.27/cm2) was detected in 37% of patients and D. folliculorum + D. brevis coinfection (total 6071, mean 134.91/cm2) in 55% of patients. Only D. folliculorum (total 97, mean 6.06/cm2) was detected in 20% of healthy controls, while D. folliculorum + D. brevis co-infection was not detected. The study showed that Demodex burden in patients with rosacea was approximately 30 times higher than in healthy controls and was caused by coinfection of D. folliculorum and D. brevis (mean Demodex burden: 179.18/cm2 in patients, 6.06/cm2 in healthy controls). It was concluded that Demodex infestation, especially co-infection with D. folliculorum and D. brevis, may be a potential factor in the aetiology of rosacea that should not be ignored in the clinic, and that it may be appropriate to initiate antiparasitic treatment when the parasite is detected.

Trends of aeroallergen sensitization among children with respiratory allergy in Southern Thailand.

Respiratory allergy significantly impacts children's health and quality of life, with inhaled allergens serving as prevalent triggers for respiratory symptoms. Aeroallergen sensitization, confirmed through the skin prick test (SPT), naturally evolves over time and is influenced by environmental factors specific to each region. This study aimed to review the updated 6-year trend of aeroallergen sensitization among children with asthma and allergic rhinitis in Southern Thailand. This retrospective study involved a review of patients aged ≤15 years diagnosed with asthma and/or allergic rhinitis, who underwent the SPT using 12 standardized aeroallergens. The medical records of 1,393 children were reviewed, with 63% being male, and the mean age was 7.4 ± 3.3 years. Among them, 55.5% presented with allergic rhinitis alone, followed by 31.6% with asthma and allergic rhinitis, and 12.9% with asthma alone. Over the 6-year period, house dust mites emerged as the most common sensitized allergen, followed closely by cockroaches, maintaining a consistent trend of sensitization. Dermatophagoides pteronyssinus ranked as the predominant sensitized allergen across all diagnoses (63% in asthma with allergic rhinitis, 52.7% in allergic rhinitis, and 43.5% in asthma). Children diagnosed with both asthma and allergic rhinitis exhibited a higher prevalence of sensitization to house dust mites, cockroaches, Johnson grass, acacia, Candida albicans, and cat pelts compared with those with asthma or allergic rhinitis alone. Additionally, the prevalence of house dust mite and cockroach sensitization was notably higher in older children (≥5 years old) with respiratory allergies. House dust mites and cockroaches emerge as the 2 most prevalent sensitized aeroallergens among children diagnosed with asthma and/or allergic rhinitis, exhibiting consistent trends of sensitization over the past 6 years. The persistence of these causative allergens underscores the importance of meticulous environmental control measures for children affected by respiratory allergies.

PREVALENCE OF ECTOPARASITE TICK AND MITE INFESTATION ON DOMESTIC CHICKEN IN BALI

The raising of free-range chickens is generally intended as laying hens, broilers, and pets by the people of Indonesia. One of the common diseases affecting free-range chickens is the infestation of ectoparasites ticks and mites that can interfere with the health of chickens and even cause economic losses on a farm. The aim of this study was to determine the prevalence of ectoparasite infestation of ticks and mites and to identify the types of ticks and mites that infest free-range chickens in Bali. The objects in this study were tick and mite ectoparasites that infested 5-6 month-old of 60 free-range chickens. Ticks ectoparasites were taken directly one by one using tweezers and for non-scabies mites were observed using a magnifying glass and taken using cotton wool soaked in 70% alcohol. Each ectoparasite taken from one individual chicken was put into an eppendorf tube filled with 70% alcohol. Identification was done by examining the preparations using a light microscope based on the CDC identification key (1966). The results of this study showed that the prevalence of ectoparasites in free-range chickens in Bali was 78% with a prevalence of ticks at 32% and a prevalence of mites at 78%. The types of ticks found were Haemaphysalis spp. and Megninia ginglymura mites. Further research is needed to identify the types of ectoparasites ticks and mites that infest chickens in Indonesia.

Prevalence of One Humped Camel Mange and Its Associated Risk Factors in Selected Kebeles of Kebri-Beyah District of Fafan Zone, Somali Regional State, Ethiopia

Camel mange is an extremely pruritic and contagious disease of camel caused by a small parasite Sarcoptes scabiei var cameli, It mostly affects the head, neck, flanks, inner side of the thighs and inguinal region of the animal. It is transmitted by direct or indirect contact, is one of the most important parasitic diseases affecting camels. Camel mange is economically important disease that hinders productivity and health of camel. Therefore, a cross sectional study was conducted between March to September 2021 to determine prevalence of camel mange mites and associated risk factors. A total of 384 animals were randomly selected and subjected to skin scrapings to recover mange from suspected lesions which was later on examined under microscope, out of the complete camels examined 116 (30.2%) camels were found positive for mange mite infestation. Only Sarcoptes scabiei var cameli was identified as the only mite species in all skin scraping samples collected from the suspected mange mite lesions. Peasant association, sex and age showed no significant variation on mange infestation (P>0.05), while only body condition and herd size were shown statistically significant difference in prevalence of mange mites (P<0.05). This study indicates that camel populations in Kebribeyah district had large amount of mange mites which could hamper health and production status of the camels. Therefore, more emphasis should be given to improve the management system, further studies and control measures should be conducted to shrink the effect of mange mite infestation on camel husbandry.

New infestation records of two-spotted spider mites on potato tubers under storage conditions and response of genotypes at Kulumsa, Ethiopia

The study sought to ascertain spider mite prevalence, infestation, and identification and to assess the response of potato genotypes. In 2022, this pest was only observed on some genotypes in December. In late January, an infestation, incidence, and severity increased from 10 to 13 genotypes on potato tuber stocks stored for irrigation experiments. Those genotypes were planted in irrigated fields, and the sprouted tuber losses ranged from 12.5% to 100% and up to 15.38% in the 2022 and 2023 records, respectively. Following this, spider mites, often known as two-spotted spider mites (TSSM) or Tetranychus urticae, were identified. This is the first time a potato sprout infestation has been recorded in Ethiopia. The two-spotted spider mites damaged the tubers quantitatively by sucking the moisture of sprouts up to the 5th grade, with 97 and >75% infestation levels, incidence, and severity, respectively. The pest grows very rapidly and hastens the drying of sprouted tuber leaves. Some genotypes were resistant to TSSM and recovered after the sprouts were infected and dried, which were treated with pesticides in 2023. The typical agricultural insecticides were ineffective in controlling the pest in the first year, and a Profenofos was applied in the second year. Thus, seed tubers are the main production limit unless appropriate research efforts are undertaken and management techniques are created. Int. J. Agril. Res. Innov. Tech. 13(2): 55-63, Dec 2023

The prevalence of IgE positivity, environmental factors, and clinical implications associated with mite species in allergic patients in Taiwan.

Allergic diseases are a growing public health concern with increasing prevalence and severity. Allergens play significant roles in triggering immune responses and the development of allergic reactions. Investigate the presence and clinical significance of dust mites, storage mites, and predatory mite Cheyletus eruditus(Ce) in household environments. A survey of household dust was performed to determine mite occurrence and analyze influencing factors, an analysis of the correlation between mite species and allergic symptoms, and basophil activation triggered by mite allergens. Cross-reactivity between Ce and house dust mites was assessed. The high appearance rate of mite species in households of Taiwan was Dermatophagoides pteronyssinus (Dp) and D. farinae(Df). Environmental factors such as pet keeping, vacuum cleaner usage, air conditioner usage, proximity to the kitchen, cleaning frequency, and protein concentration in beds were shown to influence mite prevalence. The appearance of Dp and Df significantly increased the occurrence of airway and nasal symptoms, while the presence of Ce was strongly correlated with skin symptoms. The activation of basophils and the correlation between specific IgE levels and allergic symptoms in response to Ce exposure were demonstrated. The presence of Ce was associated with elevated levels of allergens in bedding. The IgE adsorption between mite species was demonstrated suggesting cross-reactivity between the Ce and Dp was limited. Presence of Ce is associated with elevated levels of major mite allergens in beddings. Allergenicity of Ce was confirmed by IgE reactivity and basophil activation regarding mite infestation as a potential cause of skin-related allergy.

Seasonal and spatial patterns of infestation with ectoparasitic mites on New Zealand geckos revealed using a crowd‐sourced citizen science database

AbstractThe advent of online crowd‐sourced nature databases provides an opportunity to yield information about ecological interactions on a scale not previously possible. In particular, such databases provide an opportunity to collate information on host–parasite interactions through screening photographs of hosts. Using photographs from iNaturalist we expanded the known hosts for ectoparasitic mites in New Zealand geckos from 9 to 20 host species, including the first report of ectoparasitic mites on geckos from the genus Toropuku. Additionally, spatial and seasonal variation in mite prevalence was investigated, giving deeper insight in the possibilities of analysing crowd‐sourced datasets.

Gut microbiota-driven regulation of queen bee ovarian metabolism.

With the global prevalence of Varroa mites, more and more beekeepers resort to confining the queen bee in a queen cage to control mite infestation or to breed superior and robust queen bees. However, the impact of such practices on the queen bee remains largely unknown. Therefore, we subjected the queen bees to a 21-day egg-laying restriction treatment (from the egg stage to the emergence of adult worker bees) and analyzed the queen bees' ovarian metabolites and gut microbiota after 21 days, aiming to assess the queen bees' quality and assist beekeepers in better hive management. Our findings revealed a significant reduction in the relative expression levels of Vg and Hex110 genes in the ovaries of egg laying-restricted queen bees compared to unrestricted egg-laying queens. The diversity of gut microbiota in the queen bee exhibited a notable decrease, accompanied by corresponding changes in the core bacteria of the microbial community, the relative abundance of Lactobacillus and Bifidobacterium increased from 22.34% to 53.14% (P = 0.01) and from 0.053% to 0.580% (P = 0.04), respectively. The relative abundance of Bombella decreased from 25.85% to 1.720% (P = 0.002). Following egg-laying restriction, the activity of the queen bee's ovaries decreased, while the metabolism of glycerophospholipids remained or stored more lipid molecules, awaiting environmental changes for the queen bee to resume egg laying promptly. Furthermore, we observed that Bombella in the queen bee's gut may regulate the queen's ovarian metabolism through tryptophan metabolism. These findings provide novel insights into the interplay among queen egg laying, gut microbiota, and ovarian metabolism. IMPORTANCE With Varroa mite infestation, beekeepers often confine the queen bee in cages for control or breeding. However, the impact on the queen bee is largely unknown. We evaluated queen bee quality by restricting egg laying and analyzing ovarian metabolites and gut microbiota. In this study, we provided a comprehensive explanation of the expression of ovarian genes, the diversity of gut microbiota, and changes in ovarian metabolism in the queen bee. Through integrated analysis of the queen bee's gut microbiota and ovarian metabolism, we discovered that the gut microbiota can regulate the queen bee's ovarian metabolism. These findings provide valuable insights into the interplay among egg laying, gut microbiota, and the reproductive health of the queen bee. Understanding these relationships can contribute to the development of better strategies for Varroa mite control and queen bee breeding.

Prevalence of House Dust Mites and Presence of Der p 1 and Der f 1 in Ordu, Giresun, Trabzon and Rize Provinces.

This study was carried out to detect house dust mites in houses and to investigate group 1 antigens of Dermatophagoid species in Ordu, Giresun, Trabzon and Rize provinces of the Central and Eastern Black Sea Region. Dust samples obtained from the beds were subjected to both microscopic and antigenic examination. Samples prepared by the lactic acid method for microscopic examination were evaluated under a light microscope. Antigenic analysis was performed by investigating Der p 1 and Der f 1 belonging to D. pteronyssinus and D. farinae by ELISA test. 90.3% of the dust samples were evaluated positive by microscopic examination (10x, 40x) and 149 mites were detected. D. pteronyssinus 74%, D. farinae 13%, Dermatophagoides spp. growth forms 5%, Cheyletus spp. 1%, E. maynei 1%, C. arcuatus 1%, T. putrescentiae 1%, L. destructor 1% and unidentified mites were detected at the rate of 3% respectively. Der p 1 antigen was detected in 93% and Der f 1 antigen in 84.7%. The highest amount of antigen detected in one gram of powder was 1,272 μg for Der p 1 and 0,482 μg for Der f 1. No difference was observed between mite species and distribution in the provinces where the study was conducted (p<0.05). Dermatophagoides were found in 93% of the population. The low (4%) rate of storage/food mites is related to the fact that samples were not taken from the floors. Antigen accumulation may be important in the beds since the activity of the mites is observed throughout the year in temperate and humid regions. It is thought that this diagnosis method can be used and can be taken into account in terms of the environments in which sensitive people live.

Prevalence of chigger mites and Orientia tsutsugamushi strains in northern regions of Gangwon-do, Korea.

The present study aimed to survey the prevalence of chigger mites and Orientia tsutsugamushi (O. tsutsugamushi) infection in the northern regions of Gangwon-do, Korea. From early February to early June 2015, a total of 17,050 chiggers were collected from striped field mice, Apodemus agrarius, in Cheorwon-gun, Hwacheon-gun, Yanggu-gun, and Goseong-gun, which are well-known endemic areas of scrub typhus in Korea. The chiggers were analyzed using molecular genomic methods, as previously described. Among the 7,964 identified chiggers, the predominant species was Leptotrombidium pallidum (76.9%), followed by L. zetum (16.4%), L. orientale (4.3%), L. palpale (0.3%), L. tectum (0.2%), and Neotrombicula tamiyai (1.8%). The chigger index (CI) was highest in Hwacheon (115.58), followed by Cheorwon (97.02), Yanggu (76.88), and Goseong (54.68). Out of the 79 O. tsutsugamushi-positive chigger pools, 67 (84.8%) were identified as the Boryong strain, 10 (12.7%) as the Youngworl strain, and only 2 were the Jecheon strain. Based on the high infestation of chiggers in striped field rodents and the high rate of O. tsutsugamushi infection in chigger mites, Hwacheon-gun and Cheorwon-gun are presumed to be high-risk areas for scrub typhus. Furthermore, L. pallidum, a major vector of scrub typhus, and the dominant O. tsutsugamushi serotype, the Boryong strain, were found in the northern regions of Gangwon-do, Korea.

Zihinsel engelli bireylerde Demodex akar (Acari: Demodecidae) prevalansı ve yoğunluğu

This study was conducted to determine the prevalence and intensity of D. folliculorum and D. brevis in mentally disabled individuals receiving care and support services in rehabilitation centers in Erzincan province. The study included 217 mentally disabled participants who were receiving care and support services in 7 separate rehabilitation centers operating in the provincial center of Erzincan. Sample materials were taken from the cheek, nasolabial and chin area of each participant by Standard Superficial Skin Biopsy (SSSB) method and examined for the presence and number of Demodex mites under a light microscope. In the study, D. folliculorum (mean 8.34/cm2) was detected in 58% of mentally disabled people, D. brevis (mean 1.26/cm2) in 25%, and Demodex mites (mean 9.59/cm2) was detected in 61% of the all participants. When the participants were evaluated in terms of Demodex prevalence, it was determined that D. folliculorum was approximately 2.3 times more common than D. brevis. Similarly, when evaluated in terms of the average number of mites per cm2, it was found that D. folliculorum was about 7 times denser than D. brevis. On the other hand, it was determined that the prevalence of Demodex mites increased as the severity of intellectual disabilities increased, while it was found that the intensity of Demodex was highest in participants with moderate mentally disabled. As a result, it was found that D. folliculorum and D. brevis were widespread and intense in both healthy individuals and mentally disabled individuals, as well as in many patient groups. It was thought that the findings obtained in our study might be useful to consider mentally disabled individuals with dermatological complaints during the clinical evaluation process.

Insights into varroa mite (Varroa destructor) infestation levels in local honeybee (Apis mellifera) colonies of Ethiopia

AbstractThe varroa mite (Varroa destructor) is the most economically important parasitic pest of honey bees (Apis mellifera) that causes global colony decline and loss. Since its first detection in Ethiopia in 2010, little is known about the current infestation levels and contributing factors to the mite's distribution across the country. In this study, we conducted a large‐scale assessment of varroa prevalence and infestation levels in managed colonies across three potential beekeeping regions of Ethiopia; Oromia, Amhara and Southern Nations, Nationalities and Peoples Region (SNNPR). A total of 360 samples from 39 apiaries were collected following standard protocols and the mite prevalence and infestation levels were determined using the established method of varroa research. Overall, 89% of sampled colonies were infested by the mite, with varying prevalence rates across the regions: 95.8% in Oromia, 85.2% in Amhara and 71.9% in SNNPR, indicating the widespread nature of the parasite throughout the country. The varroa infestation levels were lower in local hives (2.6 ± 5.9) than in frame hives (5.0 ± 0.9), and significantly higher in brood bees (5.6 ± 0.8) than in adult bees (1.93 ± 0.17) (p &lt; 0.001). Possible factors contributing to the varied mite prevalence and infestation levels in the country include differences in agro‐ecology, beekeeping practices, host age preference and the nature of bee hives. Therefore, our study provides insights into the current status of varroa prevalence and associated factors across regions in Ethiopia. Further investigation is required to explore the probable impact of the parasite on the health and productivity of local honey bees, in order to design future monitoring strategies.

Refining the Diagnostic Technique to Determine Prevalence of Demodex Mites in Eyelash Hair Follicles Within Standard Ophthalmic Practice: A Single-Center Outpatient Clinical Study.

A high density of Demodex infestation in human eyelids and eyelash follicles has been implicated in a variety of ocular surface conditions. However, Demodex infestation often goes undiagnosed and untreated, due to an overlap in signs and symptoms with other sources of ocular surface inflammation. We sought to refine the diagnostic technique for outpatient assessment for Demodex infestation and determine prevalence of Demodex mites in the hair follicles of eyelashes from patients in a standard ophthalmic practice. Patients recruited from a single outpatient ophthalmology clinic were examined for the presence of Demodex mites following standard ophthalmic consultation. During anterior segment biomicroscopic examination, investigators searched for cylindrical dandruff and collarettes around the base of patient eyelashes. These were removed, and individual eyelashes manipulated with tweezers to reveal Demodex mites. Presence of Demodex, cylindrical dandruff and collarettes, time taken to identify the first Demodex mite, mean number of Demodex mites per hair shaft, and patient-reported symptoms of blepharitis were recorded and analyzed to determine potential correlations. A total of 173 patients were recruited, of whom 106 were included in Demodex prevalence analyses. Demodex infestation was identified in 100/106 (94%) patients, with a mean time of 2 minutes and 20 seconds taken to find the first Demodex mite and a mean number of 3 mites identified per hair shaft. Presence of Demodex significantly correlated with the presence of cylindrical dandruff (98/106 [92%] patients; p < 0.001), but not with the presence of collarettes (62/106 [58%] patients; p = 0.230) or symptoms of blepharitis (15/106 [14%] patients; p = 0.591). These results highlight the pervasiveness of Demodex infestation, predicted by the presence of cylindrical dandruff, in the eyelashes of patients in a standard ophthalmic practice. They support the use of a non-invasive diagnostic technique to allow fast, simple identification of Demodex by ophthalmologists and optometrists in outpatient clinical practice.

Assessment of Camel Mange Mite Prevalence In Kumbi Woreda, Eastern Harergae, Ethiopia

Camel mange, an exceedingly infectious ectoparasitism brought on by the parasite mite Sarcoptes scabiei and spread through direct or indirect contact, is one of the most significant parasitic disorders afflicting camels in Ethiopia. Economically significant infectious camel illness called camel mange affects the health and production of these animals. In order to ascertain the prevalence of camel mange mites and related risk factors in Kumbi woreda in Eastern Hararghe, Ethiopia, a cross-sectional study was carried out between May and November 2019. A total of 384 animals were randomly chosen for this investigation, and 96 camels tested positive for mange mite infestation. Skin scrapings were used to recover mange from suspicious lesions, which were then inspected under a microscope. As a result, 25% of camels were discovered to have mange mites. In every skin scraping sample taken from the suspected mange mite lesions, only Sarcoptes scabiei var. cameli mite species was found. PA, sex, age, physical condition status, and herd size were thought to be risk factors. Only the herd size and body condition shows a statistically significant difference in the prevalence of mange mites (P 0.05), whereas the PA, sex, and age did not exhibit any significant differences in mange infestation. According to this study, mange mites are present in camel papulations in the Kumbi woreda, which may negatively impact the health and productivity of the camels. To lessen the impact of mange mite infestation on camel husbandry, more emphasis should be placed on improving the management system, along with additional research and control measures.