An experimental study was conducted with the objective to assess the pre-test and post-test knowledge on organ donation, to evaluate the effectiveness of awareness programme on organ donation among the B. Sc Nursing students of Arya nursing college, to find out the association between pre-test knowledge on organ donation and selected demographic variables. The students were selected from B. Sc Nursing 2nd year by using purposive sampling technique. Total 50 sample were selected and self-structured questionnaires were used to collect the data and analyzed according to the study objective. Descriptive and inferential statistics were used for data analysis. The study findings showed that 8% respondents belong to age group of 17-19 years, 86% belongs to the age group of 20-22 years and 6% belongs to the age group who were more than 22 years. 52% belongs to Hindu religion, 36% belongs to Islam religion, 12% belongs to Christian religion. 84% resides in rural area and 16% resides in Urban area. 14% had attended training programme on organ donation while 86% did not. In pre-test 14% have inadequate knowledge, 74% have moderate knowledge and 12% have adequate knowledge regarding organ donation and in post-test 8% have inadequate knowledge, 72% have moderate knowledge and 20% have adequate knowledge. The mean knowledge score of pre-tests is 14.32 and standard deviation is 3.30. The mean knowledge score of post-tests is 21.66 and standard deviation is 3.14. Hence, it shows that there is increase in mean post-test knowledge score when compared to mean pre-test knowledge score.