In this study, in-situ static and dynamic tests of four pre-stressed concrete (PC) track beams with different span lengths and curvatures in a straddle monorail transit system were reported. In the static load tests, the strain and deflection at critical sections of the PC track beams were measured to determine the load bearing capacity and stiffness. The dynamic responses of strain, deflection, acceleration, and displacement at key positions of the PC track beams were measured under different train speeds and train loads to systematically study the dynamic behaviors of the PC track beams. A three-dimensional finite element model of the track beam-vehicle coupled vibration system was established to help understand the dynamic behavior of the system, and the model was verified using the test results. The research results show that the curvature, span length, train speed, and train loads have significant influence on the dynamic responses of the PC track beams. The dynamic performance of the PC track beams in the curve section is susceptible to dynamic loads. Appropriate train loads can effectively reduce the impact of the train on the PC track beam. The PC track beams allow good riding comfort.