The main goal of this study was to assess the yield and the antimicrobial activity of extracts from Cyperus articulatus L. var. articulatus obtained by pressurized carbon dioxide based on their system phase diagram behavior. The extractions were carried out at 313, 323, 333K temperatures and, 13 and 25MPa pressures. The extracts were quantified and chemically characterized by using gas chromatography coupled to mass spectrometry technique. The extracts obtained at the following experimental conditions: 333K and 13MPa, showed antifungal activity against Cladosporium sphaerospermum ATCC 4464. At 323K – 25MPa, and 333K – 25MPa, the extracts showed antibacterial activity against Staphylococcus aureus ATCC 25923. To describe the kinetics of extraction with a packed bed, a mathematical model was employed highlighting the transference mechanisms for masses in the pseudo-binary system as follows (1) carbon dioxide and (2) priprioca extract, the monophasic and multiphasic regions.