Abstract
This study focused on the use of pressurized CO2 as a reaction medium for the enrichment of docosahexaenoic acid (DHA) from tuna oil fatty acids via lipase-mediated esterification. Of the three lipases tested, Lipozyme RM IM from Rhizomucor miehei was selected for further study. Enzyme loading, water addition, and reaction time were also explored. Near-supercritical CO2, prepared at 25°C and 8.3MPa, was the most effective reagent tested for enriching DHA from the residual fatty acid fraction. In addition to near-supercritical CO2, optimal conditions included addition of 0.2wt% (based on total substrates) water, enzyme loading of 5wt% (based on total substrates), and a reaction time of 18h. The DHA concentration and recovery yield for the residual fatty acid fraction under these optimal conditions were 75.8wt% and 81wt%, respectively.
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