Skin Preservation Research Articles

Early stage ultraviolet irradiation damage to skin collagen can be suppressed by HPA axis control via controlled CYP11B

UV rays constitute an extremely important environmental factor known to operate adaptative mechanisms that maintain biological homeostasis in the skin, adrenal glands, and the brain. The skin is extremely vulnerable to UV rays. UV rays deform collagen, the main component of elastic fibers, decreasing its normal function, and ultimately reducing skin’s elasticity. We confirmed that psychological stress occurring during the early stages of UVB-irradiation degraded collagen function by inhibiting production rather than the decomposition of collagen, thereby promoting skin aging. UV irradiation for 0–2 weeks increased the level of a stress factor, corticosterone (CORT). High-performance liquid chromatography and western blot analysis confirmed that the increase was caused by enhanced CYP11B1/2 levels during steroid synthesis in the adrenal gland. Precursor levels decreased significantly during the two weeks of UV irradiation. Skin collagen and collagen fibers reduced drastically during this time. Furthermore, the administration of osilodrostat, a USFDA-approved drug that selectively inhibits CYP11B1/2, preserved skin collagen. The mechanism underlying the reduction of CORT by osilodrostat confirmed that the amount of skin collagen could be preserved with treatment. In addition, upon suppression of the CORT receptor, the amount of collagen was controlled, and skin aging was suppressed by the hypothalamic–pituitary–adrenal axis. Therefore, this study confirmed an inverse relationship between adrenal CYP11B1/2 levels and collagen during the initial stages of UV irradiation of the skin. The findings of this study may be useful for developing new detection mechanisms for aging, following their further verification.

Anti-inflammatory effects and potential mechanisms of Mitragyna speciosa methanol extract on λ-karagenan-induced inflammation model

Introduction: Mitragyna speciosa is known to have beneficial effects, such as antinociceptive and anti-inflammatory effects. There are some components that involve in inflammatory, such as Inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2) enzymes, and also cytokines. This study aimed to investigate the anti-inflammatory effect and its mechanism of Mitragyna speciosa leaf. Methods: Carrageenan-induced rat paw edema model was used for inflammation induction. Rats were given Mitragyna speciosa leaf methanol extract at a dose of 75, 150, and 200 mg/kg. Results: Intraplantar injection of carrageenan led to the development of time-dependent peripheral inflammation, which, in turn, was a significant increase in the swelling process as measured by a plethysmometer. However, administration of EMS (75,150 and 200mg/kg) can reduce edema in a dose-dependent manner. We also demonstrated that skin histology on EMS administration significantly preserved skin thickness at 4 h after carrageenan injection. Conclusion: In conclusion, we reveal the role of Mytragyna speciosa methanol extract as anti-inflammatory and maintain skin thickness in the inflammatory process.

Milk provides the basis for an eco-friendly shorter process for skin preservation and leather manufacture

The traditional beamhouse method for tanning skins to produce leather uses copious amounts of water, large quantities of chemicals deemed to be dangerous by the public and produces large quantities of liquid waste (90 kg/ton of raw hide) that have become a significant problem due to environmental concerns. In this work, we chart the development of the use of a milk product that both preserves unwashed sheepskins and depilates them, removing the need for salt, sulfide and lime and reduces the copious amounts of water used in the traditional process. This method can completely replace salting and the first five steps of the traditional beamhouse process and the depilated skins can be taken straight to degreasing and tanning. Evaluation of the depilated skins by both eye and high-resolution microscopy showed no apparent damage to the grain or fraying of the collagen bundles. The leather produced from them had physical properties that were either identical or better than those of leather made using the traditional methods. To try and understand the process, microbial culturing of the depilation liquid routinely identified two major bacterial species ( Lactococcus lactis and Lactiplantibacillus plantarum ) and two major fungal species ( Geotrichum candidum and Yarrowia lipolytica ) among other minor species. We predict that the secretion of an unidentified mix of antimicrobial substances and extracellular enzymes from these microorganisms are responsible for the preservation and depilation of sheepskin. This procedure shows potential for further development and could result in a sustainable green beamhouse operation for the leather industry. • A completely sulfide free protocol for sheepskin depilation has been developed. • It uses dairy by-products whey or permeate to preserve and depilate over 3–4 days. • No need to lime, delime bate or pickle so water use is considerably reduced. • The resulting leather has an enhanced appearance compared to normal leather. • The leather's physical properties are the same as those of the traditional product.

Combined great toe dorsal nail-skin flap and medial plantar flap for one-stage reconstruction of degloved finger

BackgroundHistorically, the degloved finger with the total loss of nail and skin had been resurfaced with an expanded great toe wrap-around flap transfer for better outcomes. The purpose of this study aims to present our experience with a related modified design, with total preservation of a weight-bearing plantar skin. MethodFrom April 2016 to September 2019, nine fingers (5 index and 4 middle fingers) in nine cases, with skin loss to the base of the proximal phalangeal or metacarpophalangeal (MCP) joint level, were reconstructed with combined great toe dorsal nail-skin flap and medial plantar artery perforator (MPAP) flap. The dorsal great toe donor was covered with a thin groin flap, and the medial plantar site was covered with a full-thickness skin graft. The cutaneous nerves were preserved within both flaps. Standardized assessment of outcome in terms of sensory, functional, and esthetic performance of the reconstructed fingers was completed. ResultsFlap survival was achieved in all cases. The contour of the reconstructed digits was comparable with the contralateral one. The fingers were available for a mean follow-up of 28 months (ranged, 22-39 months). The mean dimension of the great toe dorsal nail-skin flap was 8.5 × 4.0 cm (ranged, 6.5 × 3.5-11.0 × 4.5 cm). The average size of the MPAP flap was 6.5 × 4.5 cm (ranged, 5.0 × 3.5-8.0 × 5.5 cm). At the last follow-up, the functional and aesthetic consequences were satisfactory, as well as the restored sensory. ConclusionReconstruction of degloved fingers with the great toe dorsal nail-skin flap combined MPAP flap, preserving a plantar weight-bearing area, results in excellent contour and functional outcome. Donor-site morbidity in the foot was minimal.

Early history of skin preservation and transplantation; the role of Carl August Ljunggren.

During the late 19th and the early 20th century there was an unprecedented development in medical research. Tissue and cell culture rapidly developed into areas with many contributing scientists. The same is true for tissue transplantation. When these achievements are described afterwards in a historical context and a mainline development is constructed, there are researchers whose pioneering work is forgotten. The present paper attempts to correct this and to present a correct description of the start of tissue preservation and transplantation. We have traced relevant original publications in international journals between 1870 and 1920. The traditional view is that Alexis Carrel was the first He received a Nobel Prize 1912 for his work on vascular suture and the transplantation of blood vessels and organs. The same year he published an article on human skin storage and transplantation. This was more than a decade later than Carl August Ljunggren (1860-1934) who 1898 published his pioneering but long forgotten work on human skin preservation and transplantation, and with a vision of tissue banks. Our article contains a brief biography of Ljunggren, and further reconstructs the processes that resulted in the lack of awareness today of his achievements. Conclusion: Carl August Ljunggren was the first to preserve human skin in vitro for prolonged periods, followed by transplantation of the specimens to other patients. He was also the first to propose the use of tissue banks.

Subcutaneous Quadrantectomy Is a Safe Procedure in Management of Early-Stage Breast Cancer.

BackgroundA less-invasive surgery is often required today for many tumors, when oncologic radicality is strictly ensured, both to minimize hospital stay and health costs and to guarantee aesthetical results. Breast surgery for cancer has been radically changed in the last years since conservative interventions are widely performed everywhere.MethodsThe authors present 75 cases of early breast cancer, randomly treated with standard quadrantectomy and subcutaneous quadrantectomy; the totally subcutaneous surgical technique implies only a short periareolar skin incision and a complete quadrant resection with skin and subcutaneous layer preservation. Continuous data were analyzed by unpaired Student's t-test. The Chi-square test was used to cumulate categorical variables. The Kaplan–Meyer method and log-rank test were used to compare the overall survival and disease-free survival.ResultsNo difference was found among the two groups in terms of the type of tumor, overall survival (OS), disease-free survival (DFS), early complications, radicality, and mortality. The only significant differences were both found in the length of hospital stay and in postoperative breast deformity that required further intervention in some cases.ConclusionIn the era of mini-invasive surgery and quality assurance, the authors conclude that subcutaneous quadrantectomy is a safe procedure that allows less health cost and a better aesthetical result.

Skin Preservation in the Debridement of Necrotizing Fasciitis: A Demonstrative Case Report

Summary:Necrotizing fasciitis (NF) is a rare yet potentially fatal soft-tissue, polymicrobial infection. Aggressive debridement of the fascia and overlying skin as well as antimicrobial coverage constitute the mainstay of management, often leaving large skin defects. However, we demonstrate in this paper a case of a young woman who developed NF after liposuction and was treated by fascial debridement with minimal skin excision. Skin preservation will reduce the morbidity and improve the final aesthetic outcome.

Glove Dressing for Initial Management of Hand Burns.

Initial hand dressings for burned hands should be compatible with preservation of skin substitutes and grafts, splinting, and active motion. We have developed a standardized glove dressing directed at these requirements. Early experience with this glove dressing has included feasible clinical application, 7- to 10-minute application time, and 90% preservation of total active range of motion in a normal hand placed in the dressing under test conditions.

815 Translational Research in Skin of Color Spontaneous Repigmentation of Post-Chemical Burn Leucoderma

IntroductionAfter facial burn injuries to skin of color (SOC) patients, repigmentation plays a critical role in post-burn quality of life, given the acquired contrast between preserved skin and achromic scars, and that daily concealment may be difficult. While leucoderma is a well-known side-effect of deep chemabrasion with phenol, phenol-croton oil, and high concentration trichloroacetic acid, mechanisms of repigmentation are still not fully understood, and the observation of spontaneous repigmentation of a deep chemical injury in SOC has not previously been described in the literature. In our study, we present the dynamics of long-term spontaneous facial repigmentation of a Fitzpatrick V chemical burn patient, with microscopic findings of a deep chemical peel SOC animal model.MethodsWe demonstrate spontaneous repigmentation in a 37-year-old female who initially presented with depigmented areas, erosions and eschars on the face, 7 days after accidental deep chemical burn, due to self-application of 70% glycolic acid to improve acne. This is juxtaposed with a SOC porcine experiment, performed with 35% phenol, 1.6% croton-oil, to evaluate depth-of-injury, neocollagenesis band thickness in 21 days, and skin pigmentary safety. A 6mm punch biopsy taken at 21 days underwent Herovici staining to reveal the thickness of collagen type III neocollagenesis, confirm the presence of melanin in the epidermis, and evaluate the presence of melanophages.ResultsOn patient presentation, epidermolysis and areas of deep dermal burns were observed over the forehead and cheeks. At 14 days, multiple leucoderma scars persisted and initial perifollicular repigmentation was noticeable. At 28 days, most of the skin was repigmented; however, many areas had only perifollicular repigmentation and still very severe leucoderma. At 6 months, the skin still had small areas of leucoderma with folicular repigmentation. At 11 months the skin color was fully recovered, with minimal post-inflammatory hyperpigmentation (PIH) over the cheeks.As for the SOC animal model, at 7 days the eschar was detached, and the skin was achromic. At 21 days, skin pigmentation was completely restored without macroscopic signs of PIH. Dermatopathology evaluation revealed microscopic areas of melanophages, a fully recovered epidermal melanin, and a band of over 500 micra of collagen type III.Overall, on follicle-dense areas after chemical burn, we observed progressive full repigmentation over the course of 11 months on the patient, and by day 21 in the porcine SOC model.ConclusionsSpontaneous repigmentation occurred both after accidental deep chemical ablation to the face of a SOC patient, and on the porcine SOC model after deep chemical peel formula application.

Usefulness of the analysis of the average ridge width of fingerprints in archaeological research

Skin ridges (dermatoglyphs or fingerprints) are a characteristic pattern of sulci on the skin of primates which appear on the entire hand palm and on the soles of the feet. Fingerprints are unique, irremovable and invariable which allows bio-identification of specific individuals. The aim of the study was to investigate the usefulness of the analysis of the average width of the skin ridges in archaeological research by analyzing the foot and hand prints found on 7 artifacts dating from the Middle Ages.
 An attempt was made to describe the preserved skin slate prints, as well as details of the construction of the prints. The fingers used in forming the pottery were recognized. Attempts were made to read the context of individual impressions. In the study, the following features were analyzed: legibility and suitability of the left imprint for the analysis, the possibility of determining the type of figures (whorls, loops, archs), the possibility of determining the minutiae, the density of the skin ridges left on the surface of the ceramics and the width of the skin ridges left on the surface of the ceramics. The classification of fingerprint minutiae proposed by Czesław Grzeszyk (1970) was used as well as classification of epidermal ridges peoposed by Lestrange (1953) and modified by Bochenska (1964) and Rogucka (1968).
 Analysis of the material allowed to conclude that imprints left on building ceramics can be as useful as those imprinted on utilitarian ceramics, despite differences in the composition of the raw material used in production. However, they require more skill to examine, as the impressions are often incomplete which may be related to the fillers added to the mass. Minutiae were evident on the impressions examined, and single bifurcation was the most common form. The tactile figure most commonly found on the fingertip impressions was the loop. It was not possible to delineate Galton lines due to the wiping of the triple ray. Due to the high illegibility of the prints, it was assumed with a high degree of uncertainty that the fingerprints belonged to adults, but the gender could not be determined. Noteworthy were the prints printed on the brick belonging to children. On the mentioned artifact, there is a footprint of a child aged 1–3 years and a handprint of a crawling infant, which allowed us to conclude that the children were under the care of craftsmen.

Lymphaticovenular Anastomosis for Advanced-Stage Peripheral Lymphedema: Expanding Indication and Introducing the Hand/Foot Sign

BackgroundEffective lymphaticovenular anastomosis (LVA) requires identification of functioning lymphatics, which are not always visible with contrast-based imaging in advanced-stage lymphedema patients. Ultrasound (US) allows to identify preoperatively functioning lymphatic vessels even in limbs severely affected by lymphedema. Moreover, in our experience, we observed an interesting clinical sentry in advanced-stage lymphedema patients, the hand/foot sign that is analyzed in this paper. Patients and MethodsFrom January 2016 to January 2019, 76 consecutive advanced-stage secondary lymphedema patients underwent LVA. Preoperative planning included lymphoscintigraphy, indocyanine-green lymphography (ICG-L) and US. Patients’ features, the hand/foot sign (preservation of more normal skin on the dorsum of the hand or foot), lymphatic degeneration, quantitative, qualitative, and composite outcomes at 1-year follow-up were evaluated. ResultsAn average number of 3±0.1 LVA was performed in upper limb lymphedema (ULL) (range 2–5, 47 patients) and of 4±1.08 LVAs in lower limb lymphedema (LLL) cases (range 4–7, 29 patients). The composite outcome was positive in 45 cases (59.7%). The “negative” hand /foot sign was significantly associated with presence of functioning lymphatic channels. The incidence of adverse outcomes was significantly higher in patients with positive hand/foot sign. ConclusionPatients with no functioning lymphatic vessels detectable by lymphoscintigraphy and ICG-L may still have functioning lymphatic channels that can be identified preoperatively by ultra-high-frequency ultrasound and salvaged by LVA. The “hand/foot sign” is a simple clinical sentry that appears to be correlated with higher probability of being able to localize functional lymphatics for potential lymphovenous bypass surgery.

A cleaner goatskin preservation with leaf paste and powder: An approach for salinity remediation in tannery wastewater

In this study, a new skin preservation method using Clerodendrum viscosum leaf has been explored to find out an efficient alternative to the most popular salted preservation in leather processing. The effectiveness of the preservation systems was evaluated on a regular interval by measuring parameters like organoleptic properties, moisture content, bacterial count, and hydrothermal stability of preserved skins. After preservation, the goatskins were processed into crust leather and compared with different strength properties of corresponding conventionally preserved skins. The cross-sectional fiber structure was found undamaged by assessing scanning electron microscopy images. The salt-free preservation method in this study could reduce the total dissolved solids and chloride in the soaking liquor by 74% and 98% respectively. In contrast, the less-salt preservation method could reduce the total dissolved solids and chloride by 57% and 59%respectively. The overall results of this study revealed that the leaf of Clerodendrum viscosum plant can be used to preserve skin. The strength properties of the produced leathers also fulfilled the requirements of shoe upper leather. The proposed Eco-friendly preservation techniques are found comparable to a great extent with the traditional salt curing approaches. So, these can be used as viable alternative options for skin preservation in the leather processing industries.

Vegetable wastes as a bio-additive for low-salt preservation of raw goat skin: An attempt to reduce salinity in leather manufacture

Preservation or curing of hides/skins is performed as the primary step of leather processing to conserve them from putrefaction. Normally preservation is carried out using common salt (NaCl), which is discharged in the soak liquor contributing to ~ 70%, of total dissolved solids (TDS) load of entire leather manufacturing. In an attempt to reduce the TDS and chlorides, phyto-based preservation using garlic peel (Allium sativum) and white onion peel (Allium cepa) was carried out. Different concentrations of salt in combination with garlic peel and white onion peel were applied on freshly flayed goat skins based on its green weight and compared to control (40% salt). Sensory evaluation of the preserved skin was done by assessing different parameters like hair slip, putrefaction and odour. Estimation of hydroxyproline (HP) release, moisture content and microbial load were carried out at regular intervals. Skins that remained in good condition for 14days were further processed into leather and properties were examined which were found comparable to the conventionally cured skins. Hence, this cleaner curing technique helps in reducing the TDS and chlorides in the effluent, thus controlling the pollution caused by tanneries through sustainable leather processing.

Bio-intervention phyto-based material for raw goatskin preservation: a cleaner-sustainable approach.

The regular practice of using sodium chloride to preserve raw animal skin triggers increasing salinity and total dissolved solids (TDS) in the surface and groundwater during rehydration soaking operations. The process disrupts the lives of animals, plants, and human beings. This paper is focused on the phyto-based short-term preservation of goatskin to reduce salinity in tannery soaking operations. The indigenous Persicaria hydropiper leaf was investigated to assess the preservation of animal skin to diminish salinity and TDS of tannery soaking wastewater. Methanol-extracted leaf was characterized by GC-MS and FTIR for chemical composition analysis and affiliated functional groups. Fresh goatskins were preserved at the preliminary, laboratory, and pilot-scale scenarios to establish the best possible mixture and monitor the moisture and nitrogen content, shrinkage temperature, microorganism analysis, and pollution load at each level. The processed leathers derived from the preserved skins with an optimal mixture of 10% leaf paste with 8% salt and conventional 50% salt were tested for their physical strength. Finally, the modification in fiber structure due to the varieties of preserving chemicals was evaluated through a scanning electron microscope (SEM) and detected insignificant variation of leather fibers. The findings reported in this study can be applied to the industrial level and remove certain amounts of salinity and TDS from tannery soaking wastewater.

The Establishment of a High Standard Skin Bank in Taiwan as a Burn Care Pioneer

Introduction: The establishment of skin banks is especially important in providing crucial cadaveric skin for burn treatments. Although the 2015 Formosa Fun Coast Explosion Disaster resulted in tragic casualties, it raised public awareness of the importance of having a well-functioning skin bank and led to the initiation of a skin bank development project, as well as the establishment of the first high standard skin bank in Taiwan. Methods: We standardized the size of our procured skin to avoid broken or disrupted skin graft, fit properly on a Zimmer Dermacarrier® for mesh expansion, and to be convenient for storage. We also integrated gamma radiation with the glycerol stored skin method for our skin preservation which effectively eradicates all contamination. Results: From 2016 to 2020, 453,100 cm2 of skin were procured from 62 donors and stored in the NTUH skin bank; 254,000 cm2 of which were utilized in the treatment of 75 patients. Between 2016 and 2017, 141 microbiological examinations were administered with six were positive. Since the introduction of gamma radiation, no contamination has been observed hereafter in our examinations. Conclusions: We report the success of establishing a high standard and well-functioning skin bank at NTUH that supply sufficient skin allografts in Taiwan. It is the first interhospital supply of charitable skin allografts across Taiwan. Being humanitarian, our skin bank is passing great love forward.

An Anhydrous Sodium Chloride Skin Preservation Model for Studies on Keratinocytes Grafting into the Wounds.

Background. Human skin is needed for covering large body areas lost by trauma. The shortcomings of contemporary methods of skin storage are limited preservation time and high immunogenicity if allogeneic. Methods. We investigated whether long-lasting skin preservation in anhydrous sodium chloride (NaCl) may be the source of keratinocytes (KCs) for transplantation. Dehydrated skin fragments were preserved for a time frame from 1 week to 12 months. Then, skin fragments were rehydrated, and KCs were isolated. The viability of KCs was assessed in viability/cytotoxicity test. NaCl-preserved KCs were cultured for 7 days and transplanted to the dorsum of SCID mice. Results. The morphology of NaCl-preserved KCs was unaltered. KCs from all epidermal layers could be identified. All grafts were accepted by the recipients. Transplanted KCs: synthesized keratins 10 and 16 expressed antigens specific for stem cells and transient-amplifying cells, and remained HLA-I-positive. Moreover, they expressed the proliferative marker PCNA. Cells isolated from transplants remained viable and produced enzymes. Conclusions. Transplantation of KCs obtained from human skin and stored in anhydrous NaCl may be considered for the closure of extensive skin wounds. The originality of this method consists of an effective storage procedure and easy preparation of keratinocytes for transplantation.

209 A Comparison of Soft Tissue Reactions Seen with Different Bone Anchored Hearing Aid Abutments: Smooth-Titanium Vs Hydroxyapatite-Coated

Abstract Aim Bone Anchored Hearing Aids allow hearing via stimulation of the cochlear through bone conduction. These devices are largely successful; however, soft tissue reactions often hinder their benefit. The type of abutment used could contribute to complications. We aimed to investigate whether using smooth-titanium abutments instead of hydroxyapatite-coated abutments reduced the rate of soft tissue reactions and need for revision surgery. Method A retrospective cohort analysis of all patients who received a Bone Anchored Hearing Aid during a 3-year period. An electronic database was screened for skin reactions and surgical revisions. A comparison was made between patients who received a hydroxyapatite-coated abutment and smooth-titanium abutments. The same surgical technique, linear incision skin preservation surgery, was used for inserting both abutments. Results Sixty-six patients received a Bone Anchored Hearing Aid. Forty-five patients received hydroxyapatite-coated abutments and twenty-one received smooth-titanium abutments, two patients had smooth-titanium inserted bilaterally. The groups were significantly similar with regards to age and gender. Significantly more patients who received hydroxyapatite-coated abutments recorded soft tissue reactions, 77.78% vs 23.81% (p < 0.0001). Significantly more patients who received hydroxyapatite-coated abutments required surgical revision, 40% vs 9.52% (p = 0.0197). 17.14% underwent skin revision and change of abutment. 5.7% had the abutments removed and were not immediately replaced. Conclusions When utilising skin preservation surgery for Bone Anchored Hearing Aid insertion smooth-titanium abutments have a favourable complication profile; with less soft tissue reactions and subsequent need for revision surgery, in comparison with hydroxyapatite-coated abutments. The reasons behind these differences warrant further investigations.

Characterization and application of dried neem leaf powder as a bio-additive for salt less animal skin preservation for tanneries.

Sodium chloride (NaCl) is commonly used as a curing/preservative agent for raw hides and skins in tanneries and is removed through a soaking process with total dissolved solids (TDS) and other organic pollutants in effluent, causing significant pollution load to the environment. Hence, the present study evaluated to apply dried neem leaf powder (DNL) as an additive to reduce the usage of salt in skin processing and preservation. To make certain of DNL antimicrobial properties, solvent extracts were performed against proteolytic bacteria isolated from raw skins. Initial characterization of DNL revealed the presence of bioactive compounds nimbolide and dehydro salannol and acetone extract with 16.9-mm, 10-mm and 8-mm zone of inhibition against Salmonella sp., E. coli sp. and Bacillus sp. identified using phenotypic conventional biochemical screening method. Further, skin curing experiments were carried out using four different treatments of DNL (10% 15%, 20% and 25% w/w) along with 15% w/w of conventional salt to obtain an optimum concentration for pilot-scale studies. Thus, the application of optimal DNL (15%) and salt (15%) resulted in no physical changes such as smell and hair slip and was taken for further studies for hydroxyproline activity, pollution load and organoleptic properties along compared with control 40% salt. DNL-aided salt less preservation of freshly flayed goat skins at ambient condition showed no hair slip or putrefaction during the preservation period with significant reduction of TDS (86%) and chloride (71%) in soak liquors compared to conventional salt preservation and enhanced organic load requiring additional treatment. However, the application of the organoleptic, physical and hydrothermal properties of resulting leathers produced from the DNL applied skins was on par with results of leather obtained from conventional salt. Thus, our results demonstrate DNL-aided salt less preservation method is able to reduce the amount of salt for preservation of goat skins significantly, leading to reduced salinity issues during leather processing.

More severe stress markers in the teeth of flanged versus unflanged orangutans (Pongo spp.).

We compared an early life stress indicator, linear enamel hypoplasia (LEH), in the canine teeth of two male orangutan (Pongo spp.) morphs. Flanged males have large bi-discoid cheek pads and a laryngeal throat pouch, and they exhibit either the same or higher levels of the stress hormone cortisol throughout development compared with unflanged males, which lack secondary sexual characteristics. Such "developmental arrest" is hypothesized to either reflect a response to experienced high stress (Hypothesis 1), or an adaptation to avoid elevated stress levels and/or having experienced lower stress levels (Hypothesis 2) during early life. As LEH defect depth has been shown to reflect the severity (i.e., intensity and/or duration) of early life stress events, we examined whether unflanged males have shallower LEH defects than flanged males. Flanging status was assessed by measuring the faces of preserved skins. Canine height (N=37) was measured in the same individuals to assess commonality between morphs. LEH defect depths were analyzed using a standardized confocal profilometry method (N=34). Flanged males have significantly deeper LEH defects than unflanged adult males. Canine projected crown heights are similar across males regardless of morph. Evidence from great apes shows that, when comparing canines with similar growth patterns, deeper defects reflect more severe stress events during development. Thus, our results suggest that "developmental arrest" of unflanged males is not a response to having experienced stress, but rather an adaptation to avoid the physiological impacts associated with chronic stress and/or experiencing lower stress levels.

Glycerolised Skin Allografts for Extensive Burns in Low- and Middle-income Countries.

Introduction:There has been a significant improvement in the outcome of treatment of large surface area burns in developed countries. A major contributory factor is an early excision and skin grafting of burn wounds. The initial coverage of large surface area deep burn wounds requires the use of temporary skin substitutes such as allografts due to limited skin autografts. Cadaveric skin allografts are the commonest source of skin allografts in use; however, there may be religious, cultural, cost, or other factors mitigating its availability and routine use in low- and middle-income countries (LMICs). Human skin allografts may be used fresh or stored in tissue banks to ensure its ready availability. The purpose of this review is to promote glycerolised skin allografts as a means of skin preservation in low-resource countries above other modalities cryopreservation due to its cost advantages and relative ease of operation.Materials and Methods:A literature search for articles related to human skin allograft use in burn care, skin banks, and glycerolised skin allografts in LMICs was done using PubMed, EMBASE, and Web of Science databases. The key words used were ‘allograft’ and ‘burn’ with a filter in the search for human studies. The relevant references in the articles obtained were also searched for and included in the reviewResults:Sixty-three journal articles were reviewed for contents in line with the objectives of this study.Conclusion:Glycerolised skin graft is a viable option for coverage of extensive burns in LMICs.