Flavonols aglycones are well established in plant extracts with omnipresence of common flavonols like kaempferol, quercetin and myricetin. Quercetin has been reported for various pharmacological activities with major ones in the field of inflammation, cancer, arthritis, diabetes and its complications. Leaves of plants selected for this study viz. Allium cepa L., Ficus carica L. and Ziziphus mauritiana L. reported to have antioxidant, anticancer, hypoglycemic activities. It is hypothetised thisactivities are due to flavonoids present in these plants. Hence the ethylacetate fraction of methanolic extract of these leaves was obtained as it provides flavanoid rich fraction and the samples were prepared and HPTLC analysis was performed against standard quercetin and their quantitative analysis was performed, the solvent system toluene: ethyl acetate: formic acid (9: 1: 0.5) was selected. It was observed that these plants shows presence of quercetin where A. cepa has highest concentration with F. carica and Z. mauritiana having quercetin in declining order. The calibration curve was linear in the range of 0.1 to 0.7 μg for quercetin. Further a correlation coefficient of 0.999 indicates good linearity between concentration and area. Thus, it was proved that the plants used in the study has presence of quercetin which is possibly the reason having the pharmacological uses.