Presence Of Fe3O4 Nanoparticles Research Articles

Enhancement of exopolysaccharide production by Stenotrophomonas maltophilia and Brevibacillus parabrevis isolated from root nodules of Cicer arietinum L. and Vigna unguiculata L. (Walp.) plants

This study intended to determine the ability of endophytic bacteria recovered from root nodules of Cicer arietinum L. and Vigna unguiculata L. (Walp.) plants to synthesize exopolysaccharide (EPS) and to enhance the production by changing nutritional factors. Twenty endophytic bacteria isolated from root nodules of Cicer arietinum and Vigna unguiculata were tested for their production of EPS. High EPS-producing isolates were identified on phenotypic and genotypic characteristics. Among 20 isolates, Stenotrophomonas maltophilia (C6) and Brevibacillus parabrevis (V4) isolated from root nodules of Cicer arietinum and Vigna unguiculata produced a high EPS yield in comparison with other isolates. Using 1% of sucrose as sole carbon source increases the concentration of EPS produced by S. maltophilia and B. parabrevis (65 and 107%, respectively). EPS produced by S. maltophilia and B. parabrevis was increased by the addition of fructose and lactose (0.1%). Addition of 1.68 g/L KNO3 or 2.49 g/L glycine to modified yeast extract mannitol medium (YEMB) significantly increased EPS production by S. maltophilia and B. parabrevis. Furthermore, the presence of Fe3O4 nanoparticles (25–50 µg/mL) in the modified YEMB medium increased EPS yield by B. parabrevis. Chemical characterization of EPS by GC–MS and FTIR indicate that the EPS biochemical composition is dependent on the bioavailability of carbon substrates and is controlled by limiting nutrients. The combination of the best two carbon sources sucrose (0.9%) and fructose or lactose (0.1%) in the presence of KNO3 or glycine as the best nitrogen sources significantly increased EPS yield of S. maltophilia and B. parabrevis, respectively.

Graphene oxide (rGO)-metal oxide (TiO2/Fe3O4) based nanocomposites for the removal of methylene blue

Herein, ternary nanocomposites based on titanium dioxide, ferric oxide and reduced graphene oxide (GO) have been developed for photocatalytic degradation of methylene blue. The nanocomposites are prepared by simple sol-gel and wet assembly methods with varying weight ratio of each components to obtain efficient photocatalytic degradation. Due to the synergistic effect among the three components, a swift removal of methylene blue becomes possible under visible and UV light. The rGO-Fe3O4-TiO2 nanocomposite having composition 1:1:2 has achieved maximum degradation of methylene blue from the aqueous solution. About 99% of the dye has been removed within 6 min under UV irradiation, while in presence of visible light, 94% has been degraded from the wastewater. The enhancement of photocatalytic activity in this ternary system is attributed to the efficient separation of charge carriers from TiO2 to rGO under the exposure of light and the initiation of photo-Fenton reaction due to the incorporated Fe3O4 nanoparticles in presence of H2O2, which provides highly reactive hydroxyl ions that mineralize the pollutants. All these results indicate that these ternary nanocomposites possess great potential for both UV and visible light driven methylene blue destruction from the wastewater.

Iron Oxide Nanoparticles as Nano-adsorbents: A Possible Way to Reduce Arsenic Phytotoxicity in Indian Mustard Plant (Brassica juncea L.)

Application of nanoparticles (NPs) is very effective in reducing metal toxicity. The present study was designed to determine the effectiveness of iron oxide nanoparticles (Fe3O4 NP) in reducing the toxicity of arsenic in Indian mustard (Brassica juncea var. Pusa Jagannath) plant. Fourteen-day-old mustard plants were subjected to 150 µM As(III), Fe3O4 NPs (500 mg L−1 Fe3O4), 500 mg L−1 FeSO4 or As(Ш) + Fe3O4 NPs (150 µM + 500 mg L−1) stress for a period of 96 h. Significant toxicity was observed in seed germination indicators, and root–shoot length under arsenic (As) stress, but application of Fe3O4 NPs along with As improved the overall growth of plant. Results demonstrated increased photosynthetic pigment and protein content under Fe3O4 NP augmentation as compared to As-alone treatment. Antioxidative enzymes such as SOD, CAT, APX and stress-related parameters (cysteine and proline) showed varied results under different treatments. However, decreased stress-related parameters might be due to restricted entry of As inside the plant in the presence of Fe3O4 NP, and hence detoxification machinery is not required. The ameliorating effect of Fe3O4 NPs in combination with As was confirmed by reduced MDA and H2O2 content. Further, the addition of Fe3O4 NPs along with As altered sulphur-related gene transcripts. Overall, this study suggests the possible involvement of Fe3O4 NPs as nano-adsorbents in reducing As toxicity in the plant through its size variation and increase/decrease of various study parameters.

One step synthesis of Fe3O4/GO nanocomposites at 100°C and its magnetic properties

ABSTRACTIn the research work, we had planned a one-step procedure to prepare magnetite/graphene oxide (Fe3O4/GO) nanocomposites by using chemical co-precepitation method. The polycrystalline phase formation of the magnetite nanoparticles was confirmed by using X-ray diffraction setup. Structural and surface morphology properties of Fe3O4/GO nanocomposites are analysed by scanning electron microscopy and transmission electron microscopy. The Raman spectrum of Fe3O4/GO nanocomposites shows the enhancement of D and G band, which are associated with GO sheets due to the presence of Fe3O4 nanoparticles. Magnetic property measurement was carried out by physical property measurement system (PPMS), which shows its superparamagnetic behaviour.

Revisiting the origin of cycling enhanced capacity of Fe3O4 based nanostructured electrode for lithium ion batteries

We identify that reversible formation/decomposition of lithium oxide, pulverization of Fe3O4 nanoparticles, and electrolyte reactions, are contributors to the enhanced capacity observed in the Fe3O4 electrode upon long cycling. Introducing three-dimensional graphene foam to form a composite with Fe3O4 nanoparticles largely increases the capacity (~ 1220mAhg−1 vs. ~ 690mAhg−1) and promotes the cycling induced capacity enhancement (an earlier capacity rise and a faster rising rate) of the Fe3O4 electrode. Together with Fe3O4 nanoparticles, the presence of graphene effectively promotes the electrolyte reactions and reversible formation/decomposition of lithium oxide. At the same time, activation of GF also occurs in the presence of Fe3O4 nanoparticles, further increases the capacity of the nanocomposite.

Co-metabolic removal of ciprofloxacin under condition of interaction between microbes and Fe3O4

Potential of effluents from biological hydrogen production process to improve ciprofloxacin (CIP) removal was investigated using ciprofloxacin-degrading enrichments in the presence of Fe3O4 nanoparticles. Effluent addition clearly promoted CIP removal, which was 61%, 38% and 16% higher than those in the enrichments amended with fecal sewage, biogas digestate and water, respectively. Experiments of sterilization and organic components of effluents indicated that the organic substances served as co-metabolic substrate to facilitate CIP removal, where ethanol was the preferred co-metabolic substrate. Although the effluent addition changed the bacterial community composition which corresponded to the changes in the relative abundances of dominant genera in the enrichments, CIP removal performance remained unchanged. The increased Fe (II) concentrations indicated that there might be a positive relationship of CIP removal with the microbial reduction of Fe (III) to Fe (II) in Fe3O4 in the effluents-amended enrichments. It was proposed that the organic substances in the effluents might serve as the co-metabolic substrate to promote CIP removal by CIP-degrading and Fe (III)-reducing bacteria.

A comparative study on the morphology of P3HT:PCBM solar cells with the addition of Fe3O4 nanoparticles by spin and rod coating methods

Our previous study presented up to 20% power conversion efficiency (PCE) enhancement of poly(3-hexylthiophene):phenyl-C61-butyric acid methyl ester (P3HT:PCBM) solar cells under the Fe3O4 nanoparticles (NPs) self-assembly (SA) effect by spin coating. Fe3O4 NPs (about 11 nm hydrodynamic diameter) form a thin layer at the top interface of the light absorbing active layer, which results in the generation of PCBM rich region improving the charge transport (Zhang et al. Sol Energ Mat Sol C 160:126–133, 2017). In order to investigate the feasibility of this Fe3O4 NPs SA effect under large-scale production condition, a smooth rod was implemented to mimic roll-to-roll coating technique and yield active layers having about the same thickness as the spin-coated ones. Small angle neutron scattering and grazing incidence X-ray diffraction were employed finding out similar morphologies of the active layers by these two coating techniques. However, rod-coated solar cell’s PCE decreases with the addition of Fe3O4 NPs compared with the one without them. This is because PCBM rich region is not created at the top interface of the active layer due to the absence of Fe3O4 NPs, which is attributed to the weak convective flow and low diffusion rate. Moreover, in the rod-coated solar cells, the presence of Fe3O4 NPs causes decrease in P3HT crystallinity, thus the charge transport and the device performance. Our study confirms the role of spin coating in the Fe3O4 NPs SA effect and enables researchers to explore this finding in other polymer nanocomposite systems.

Responses of Periphyton to Fe2O3 Nanoparticles: A Physiological and Ecological Basis for Defending Nanotoxicity.

The toxic effects of nanoparticles on individual organisms have been widely investigated, while few studies have investigated the effects of nanoparticles on ubiquitous multicommunity microbial aggregates. Here, periphyton as a model of microbial aggregates, was employed to investigate the responses of microbial aggregates exposed continuously to Fe2O3 nanoparticles (5.0 mg L-1) for 30 days. The exposure to Fe2O3 nanoparticles results in the chlorophyll (a, b, and c) contents of periphyton increasing and the total antioxidant capacity decreasing. The composition of the periphyton markedly changes in the presence of Fe2O3 nanoparticles and the species diversity significantly increases. The changes in the periphyton composition and diversity were due to allelochemicals, such as 3-methylpentane, released by members of the periphyton which inhibit their competitors. The functions of the periphyton represented by metabolic capability and contaminant (organic matter, nitrogen, phosphorus and copper) removal were able to acclimate to the Fe2O3 nanoparticles exposure via self-regulation of morphology, species composition and diversity. These findings highlight the importance of both physiological and ecological factors in evaluating the long-term responses of microbial aggregates exposed to nanoparticles.

Evaluation of Hybrid Polymeric Resin Containing Nanoparticles of Iron Oxide for Selective Separation of In(III) from Ga(III)

In this study, a novel poly(acrylic acid–dimethylaminoethylmethacrylate) in presence of Fe2O3 nanoparticles was prepared by copolymerization technique using gamma irradiation as initiator at radiation dose of 50 kGy with dose rate of 943.47 Gy/h. The obtained hybrid polymeric resin was characterized using scanning electron microscope, Transmission electron microscopy, Fourier transform infrared spectroscopy, thermal analysis (synchronized TGA–DTA) and X-Ray Fluorescence. The adsorption of In(III) and Ga(III) on the obtained hybrid polymeric resin was investigated using batch equilibrium technique with variation of pH and contact time. The batch experimental data show that, this hybrid polymeric resin has high selectivity toward In(III) than Ga(III) ions in aqueous medium. Chromatographic separation of In(III) and Ga(III) has been studied in aqueous medium. The recovery of indium and gallium from packed column was achieved using different eluents.

Conductive thin films based on poly (aniline-co-o-anthranilic acid)/magnetite nanocomposite for photovoltaic applications

In this research, magnetite particles in a nanoscale were coated with three various weight percentages of poly (aniline co-o-anthranilic acid) copolymer. Aniline and o-anthranilic acid monomers were copolymerized in the presence of Fe3O4 nanoparticles via surface initiated polymerization method. Characterization of nanocomposites was carried out by several analysis techniques including; FT-IR, FE-SEM, TEM, XRD, TGA and UV–vis-Near infrared. FT-IR results showed that pended COOH groups of PANAA are physically interacted to magnetite nanoparticles. Magnetite nanoparticles enhanced the thermal strength of the nanocomposites in the range of 300–800°C. The XRD analysis revealed that the average crystallite size ranges from 7.59 to 11.14nm depending on PANAA weight percentage. Thin films of uniform spherical shape of PANAA/magnetite nanocomposite were fabricated by thermal evaporation technique. The values of Eg(direct) and Eg(indirect) decreased with increasing the thickness of PANAA, while the conductivity increased with increasing of PANAA thickness coated magnetite particles. The conductivity value ranged from 3.73 to 26.98Ω−1cm−1 at a photon energy 3.65eV. Using the nanocomposites in photovoltaic applications was highlighted.

Partial Discharge Behaviour within Palm Oil-based Fe2O3 Nanofluids under AC Voltage

In this work, comparison studies of partial discharge (PD) behaviours within palm oil-based Fe2O3 nanofluids under AC voltage were performed. The samples were prepared with different weight percentage of Fe2O3 nanoparticles; LC (Low Concentration), MC (Medium Concentration) and HC (High Concentration) in order to investigate the „electron trapping“ effectiveness, their discharge withstand capability, discharge numbers, magnitude and Phase Resolved Partial Discharge (PRPD) patterns under high voltage stress. The results indicate that the presence of Fe2O3 nanoparticles with LC enhances the withstand characteristics of palm oil against PD.

Improving the performance of the organic solar cell and the inorganic heterojunction devices using monodisperse Fe3O4 nanoparticles

8nm Fe3O4 nanoparticles (NPs) were successfully doped into poly(3-hexylthiophene):phenyl-C61-butyric acid methyl ester (P3HT:PCBM) to fabricate ITO/PEDOT:PSS/P3HT:PCBM:Fe3O4/Al solar cell along with a heterojunction device of Fe3O4/p-GaAs by depositing them on p-GaAs substrates. The experimental results revealed that the presence of Fe3O4 nanoparticles (NPs) in the ITO/PEDOT:PSS/P3HT:PCBM/Al solar cell improved its performance with respect to the one without Fe3O4. For example, power conversion efficiency was increased from 1.09% to 2.22% when doping 5wt% of Fe3O4 NPs to P3HT:PCBM. This was attributed to increase of the light absorption in the presence of Fe3O4 NPs doping. Furthermore, the analysis of the current–voltage (I–V), capacitance–voltage (C–V) and capacitance–frequency (C–f) characteristics of the Fe3O4/p-GaAs heterojunction have been studied successfully. The experimental barrier height Φb and ideality factor n were determined as 0.80eV and 1.53, respectively, from the experimental I–V plots. In addition, the value of the Φb obtained from the C-V characteristics was 0.95eV (f=500kHz). The mismatch between barrier heights obtained from both measurements was explained by the two techniques are based on different nature. The interface state density of the Fe3O4/p-GaAs heterojunction was determined from 5.16×1014cm−2eV-1 to 1.34×1015cm−2eV−1.

RETRACTED ​ARTICLE: Synthesis of Magnetic Composites Based on Waste Low Density Polyethylene Wax and Iron Oxide Nanoparticles for Methyl Green Adsorption

Magnetic nanoparticle composites were prepared from iron oxide (magnetite) (Fe3O4) and waste low density polyethylene wax (WLDPEW) via gamma irradiation technique using 60Co source, followed by anchoring Fe3O4 on the surface of WLDPEW samples to produce WLDPEW–Fe3O4 nanoparticle magnetic composite pellets. The outcome WLDPEW and WLDPEW–Fe3O4 nanoparticle magnetic composite pellets were characterized by Fourier transform infrared spectroscopy, X-ray diffraction (XRD), scanning electron microscope-energy dispersive X-ray spectrometry, high resolution transmission electron microscopy (HR-TEM) and electron spinning resonance (ESR). The results of HR-TEM and XRD showed that the presence of Fe3O4 nanoparticles. HR-TEM photomicrographs of the Fe3O4 into WLDPEW showed that the nanoparticles of Fe3O4 have the average diameter ranged from ~30 to ~50 nm. The EDS spectra showed strong peaks of Fe and O. The ESR spectra exposed the paramagnetic properties of WLDPEW–Fe3O4 nanoparticle magnetic composite pellets. The WLDPEW–Fe3O4 nanoparticle magnetic composite pellets were exploited in adsorption of methyl green dye from aqueous solutions at various conditions. The kinetics and the adsorption isotherms were studied. Through results it was observed that the adsorption capacity of WLDPEW–Fe3O4 nanoparticle magnetic composite pellets is 18.5 mg g−1. Therefore, WLDPEW–Fe3O4 nanoparticle magnetic composite pellets could be used in removing dyes from their aqueous solutions.

In Situ Preparation of Magnetic Fe3 O4 Nanoparticles in Presence of PLGA and PVA as Magnetite Nanocarrier for Targeted Drug Delivery

Introduction: Drug targeting represents an interesting motivation to prevent side effects and increase doxorubicin cytotoxicity. Magnetic nanoparticles offer new opportunities for developing effective drug delivery systems because they are feasible to produce characterize and specifically tailor their functional properties for drug delivery applications. To improve their stability and biocompatibility, the Fe3O4 nanoparticles are often modified with surfactants or polymers. Materials and Methods: In the present work, a nanocomposite was synthesized via in situ preparation of Fe3 O4 nanoparticles inside poly lactic glycolic acid and poly vinyl alcohol. The anticancer drug of doxorubicin was loaded on the synthesized nanocomposite in order to targeted drug delivery technique. The nanostructures were characterized by FT-IR, SEM, VSM and XRD techniques. The in vitro drug release from synthesized nanocomposite was investigated as nanocarrier in 2 different pHs (equal blood and tumor environments) at 37° C and extent of drug release was calculated by UV-Vis spectrophotometer. Results: In vitro drug release experiments showed that the doxorubicin release at pH=6.0 was promisingly more and faster than pH=7.4. The fitted equation of release curves was corresponded to Peppas model. Conclusion: All these results together suggest that the DOXloaded nanocarrier may serve as a promising magnetic targeting therapy for the treatment of tumor cells.

Synthesis, characterization and adsorption properties of Fe3O4 /MWCNT magnetic nanocomposites

In this work, a simple and efficient chemical method to prepare Fe3O4 magnetic nanoparticles decorated on multi-walled carbon nanotube (Fe3O4/MWCNT nanocomposites) was presented. Fe3O4 nanoparticles were prepared by chemicalco-precipitationof Fe2+andFe3+in alkaline solution. The average particle size diameter of the particles was approximately 12 nm. A mixture between the obtained colloidal solution of Fe3O4 nanoparticles and the colloidal dispersion of acid functionalized MWCNT in water resulted in uniformly Fe3O4nanoparticles deposited on carbon nanotube. In basic solution, Fe3O4 nanoparticles were stably attracted to carboxylate groups (MWCNT-COO-) on the surface. The presence of Fe3O4nanoparticles and their surface conjugation to MWCNT have been confirmed by XRD, TEM and FT-IR techniques. The effect of Fe3O4 loading on the X-ray diffraction patterns were determined to investigate their chemical and phase composition. Magnetic evaluation of the synthesized magnet revealed a strong magnetic separation property. The sorption properties of Fe3O4/MWCNT composites for the removal of methylene blue from aqueous solution were investigated. The effect of contact time and initial concentration of MB on the adsorption were fully studied. The result kinetic data were well followed by the pseudo-second-order model.

A novel electrochemical biosensor based on Fe3O4 nanoparticles-polyvinyl alcohol composite for sensitive detection of glucose

In this research, a new electrochemical biosensor was constructed for the glucose detection. Iron oxide nanoparticles (Fe3O4) were synthesized through co-precipitation method. Polyvinyl alcohol-Fe3O4 nanocomposite was prepared by dispersing synthesized nanoparticles in the polyvinyl alcohol (PVA) solution. Glucose oxidase (GOx) was immobilized on the PVA-Fe3O4 nanocomposite via physical adsorption. The mixture of PVA, Fe3O4 nanoparticles and GOx was drop cast on a tin (Sn) electrode surface (GOx/PVA-Fe3O4/Sn). The Fe3O4 nanoparticles were characterized by X-ray diffraction (XRD). Also, Fourier transform infrared (FTIR) spectroscopy and field emission scanning electron microscopy (FE-SEM) techniques were utilized to evaluate the PVA-Fe3O4 and GOx/PVA-Fe3O4 nanocomposites. The electrochemical performance of the modified biosensor was investigated using electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). Presence of Fe3O4 nanoparticles in the PVA matrix enhanced the electron transfer between enzyme and electrode surface and the immobilized GOx showed excellent catalytic characteristic toward glucose. The GOx/PVA-Fe3O4/Sn bioelectrode could measure glucose in the range from 5 × 10−3 to 30 mM with a sensitivity of 9.36 μA mM−1 and exhibited a lower detection limit of 8 μM at a signal-to-noise ratio of 3. The value of Michaelis-Menten constant (KM) was calculated as 1.42 mM. The modified biosensor also has good anti-interfering ability during the glucose detection, fast response (10 s), good reproducibility and satisfactory stability. Finally, the results demonstrated that the GOx/PVA-Fe3O4/Sn bioelectrode is promising in biosensor construction.

Intensification of liquid-liquid mass transfer in micromixer assisted by ultrasound irradiation and Fe3O4 nanoparticles

In the present study, Cu (II) removal from aqueous solution was performed in a micromixer by using D2EHPA solvent. The impact of nanoparticles addition, ultrasound wave irradiation and combination of them for improving mixing and mass transfer was investigated. The experiments were carried out in three different ultrasound input electrical powers (3.5–7W). A constant concentration of nanoparticles (0.01w/v) was employed in a reactor consists of a circular pit at its junction, and the results were compared with the Y-type microchannel (without ultrasonic source). Depending on the mixing approach type and flow rate of the phases (1.875–6mL/min), several separate flow patterns were observed, mainly slug, droplet, dispersed and parallel flow. The mass transfer coefficient (KLa) and removal efficiency (E) enhanced up to 251% and 53%, respectively. One of the benefits of the proposed techniques is a significant reduction in pressure drop compared with simple microchannels with the same volume. The highest amount of performance ratio (1.56) was observed in the presence of Fe3O4 nanoparticles under 7W ultrasonic irradiation power.

Measurements of ionic liquids thermal conductivity and thermal diffusivity

Thermal conductivity and thermal diffusivity of ionic liquids (ILs) are investigated in this work. A hot disk method for ILs thermal conductivity and thermal diffusivity measurement is utilized. Firstly, the thermal conductivity of water is measured to check the reliability of the hot disk method. In addition, the thermal conductivity of pure ILs, including BmimBF4, BmimPF6, OmimCl, BmimFeCl4, and OmimFeCl4, is measured. By comparison with thermal conductivity values of water, BmimBF4, and BmimPF6 in the literatures, it is found that the thermal conductivity values of ILs using hot disk method have high reliability. Therefore, the hot disk method is utilized for thermal conductivity measurement of ILs in this work. The experimental results also show that all the average thermal conductivity values of the five pure ILs are no more than 0.1898 W m−1 K−1, which is much lower than the average measured thermal conductivity of water, namely 0.6033 W m−1 K−1. Effect of nanoparticles (NPs) on thermal conductivity of ILs is also investigated. It is shown that the thermal conductivity of BmimBF4 does not change significantly in the presence of Fe2O3 NPs. However, the thermal conductivity of BmimPF6 decreases somewhat in the presence of R711 NPs. In addition, the thermal diffusivity of pure ILs, including BmimBF4, BmimPF6, BmimFeCl4, OmimCl, and OmimFeCl4, is measured. All the average thermal diffusivity values of the ILs of BmimBF4, BmimPF6, BmimFeCl4, OmimCl, and OmimFeCl4 are no more than 0.1185 mm2 s−1. The thermal diffusivity of water is 0.143 mm2 s−1 in the literature. It illustrates that the ILs also have a better thermal property than water for energy storage. ILs may be utilized as novel materials for energy storage. Effect of NPs on thermal diffusivity of ILs is also investigated. The results are similar to how NPs influence thermal conductivity of BmimBF4 and BmimPF6. In the presence of Fe2O3 NPs, thermal diffusivity of BmimBF4 does not change significantly. However, in the presence of R711 NPs, thermal diffusivity of BmimPF6 decreases somewhat.

Efficient Reduction of C–N Multiple Bonds Catalyzed by Magnetically Retrievable Magnetite Nanoparticles with Sodium Borohydride

A simple, rapid and efficient methodology has been developed for the reductive transformation of the compounds bearing C–N multiple bonds such as oximes and nitriles to the corresponding amines by sodium borohydride catalyzed by highly active solid Fe3O4 nanoparticles. The catalyst was easily recovered using external magnet and reused five times without losing its catalytic activity. A simple, rapid, efficient and reusable heterogeneous catalytic system has been developed for the reductive transformation of oximes and nitriles into corresponding amines by sodium borohydride in presence of Fe3O4 nanoparticles.

One-step electrochemical deposition of Polypyrrole–Chitosan–Iron oxide nanocomposite films for non-enzymatic glucose biosensor

One-step electrodeposition method of Polypyrrole–Chitosan–Iron oxide (Ppy–CS–Fe3O4) nanocomposite films (Ppy–CS–Fe3O4NP/ITO) has been developed for the fabrication of advanced composite coatings for biosensors applications. The FESEM and EDX results provide the evidence of successful incorporation of Fe3O4 into Ppy–CS molecules. The presence of Fe3O4 nanoparticles in the nanocomposite films was further confirmed by the XRD and XPS spectrums. The fabricated electrode Ppy–CS–Fe3O4 NP/ITO shows a fast amperometric response with high selectivity to detect glucose non-enzymatically with improved linearity (1–16mM) and the detection limit of (234μM) at a signal-to-noise ratio (S/N=3.0).