Preliminary Separation Research Articles

APPLICATION OF SELECTIVE HYPROMELLOSE SORBENT FOR THE BENZOCAINE QUANTITATION IN PHARMACEUTICAL FORMULATIONS

Introduction. In quality control of pharmaceutical formulations, there are methodological approaches based on the preliminary separation of sample components and approaches without preliminary separation. The use of solid-phase extraction (SPE) allows to separate analytes during sample prepa-ration, with the separation selectivity determining the analysis selectivity as a whole. Currently, sorbents that provide selectivity in interaction with the analyte are of interest. Aim. Evaluation of the metrological characteristics of benzocaine quantitation by UV-spectrophotometry in certain pharmaceutical formulations using hypromellose sorbent for solid-phase extraction. Material and methods. The sorbent using a method developed by us was obtained. Benzocaine (FS.2.1.0634, Russia State Pharmacopoeia XV ed.) to form active binding sites in sorbent structure was applied. The sorbent structure is polycyanoacrylate matrix with hypromellose fragments, the sur-face area is 255.50 m2/g, pore volume is 0.1433 cm3, pore diameter is 5.32 nm. The sorption capacity of hypromellose sorbent for benzocaine was 12.2±0.8 μg/g. The SPE technique is proposed. SPE includes the stages: conditioning, sample addition and step-by-step elution with purified water and hydrochloric acid solution 0.1 mol/L. The spectrophotometer SF-56 was used to measure the absorbance at an analytical wavelength of 286 nm. To evaluate the selectivity (specificity) of sample preparation during chromatographic (SPE) separation of pharmaceutical formulations, absorption spectra of eluates in the wavelength range of 200400 nm were scanned. The benzocaine identification in the eluate was carried out based on absorption peaks at 220 and 286 nm. The benzocaine calibration curve in the range of 120 μg/ml. To determination the metrological characteristics of benzocaine quan-titation method, 11 parallel determinations of samples of each pharmaceutical formulation were made. Results. When realizing the benzocaine quantitation technique for measuring absorbance, it is advisable to use only the first portions of eluate (a solu-tion of hydrochloric acid) in a volume of 510 ml were obtained. The relative error of benzocaine average concentration in the pharmaceutical formula-tions ranged from 1.28 to 1.34% for technique that included the SPE stage, and from 1.86 to 2.02% for technique that did not include it. A comparison of the modifications of the spectrophotometric quantitation of benzocaine in pharmaceutical formulation using hypromellose sorbent and technique without the SPE did not reveal a statistically significant difference in reproducibility. For the modification technique without SPE, an increase of benzo-caine concentration and in determination relative error by an average of 48.0% were observed. Conclusion. The possibility of using a selective hypromellose sorbent for sample preparation for benzocaine quantitation determination in pharmaceu-tical formulations has been showed. The use of a sorbent for SPE at the sample preparation stage reduces the systematic error of benzocaine spectro-photometry quantitation for the analyzed pharmaceutical formulations by an average of 79.2%.

Influence of Partially Carboxylated Powdered Lignocellulose from Oat Straw on Technological and Strength Properties of Water-Swelling Rubber.

The work is aimed at the development of an energy-saving technique involving the partial carboxylation of powdered lignocellulose products from the straw of annual agricultural plants and the use of the obtained products in rubber compositions as a water-swelling filler. Lignocellulose powder from oat straw (composition: α-cellulose-77.0%, lignin-3.8%, resins and fats-1.8%) was used for carboxylation without preliminary separation into components. Microwave radiation was used to activate the carboxylation process. This reduced the reaction time by 2-3 times. The synthesized products were analyzed by IR spectroscopy, thermogravimetry and scanning electron microscopy. Industrial product sodium carboxymethylcellulose (Na-CMC) was used as a swelling filler for comparison. The swelling fillers were fractionated by the sieve method; particles with the size of 0-1 mm were used for filling rubber compounds. The amount of swelling filler was 150 parts per 100 parts of rubber (phr). Due to the high filling of rubber compounds, plasticizer Oxal T-92 was added to the composition of a number of samples to facilitate the processing and uniform distribution of ingredients. The rubber composition was prepared in two stages. In the first stage, ingredients without swelling filler were mixed with rubber on a laboratory two-roll mill to create a base rubber compound (BRC). In the second stage, the BRC was mixed with the swelling filler in a closed laboratory plasti-corder rubber mixer, the Brabender Plasti-Corder® Lab-Station. Vulcanization was carried out at 160 °C. For the obtained samples, the physical-mechanical and sorption properties were determined. It has been shown that the carboxylated powdered lignocellulose from oat straw increases the strength properties of rubber in comparison with Na-CMC. It has been shown that when the carboxylated powdered lignocellulose from oat straw is introduced into the rubber composition, the degree of rubber swelling in aqueous solutions of various mineralizations increases by 50 and 100% in comparison with a noncarboxylated lignocellulose.

Fast and simple method for simultaneous determination of palladium and rhodium in spent automotive catalysts by high-resolution continuum source electrothermal atomic absorption spectroscopy

This work established a novel and fast method for the simultaneous determination of palladium and rhodium in spent automotive catalysts by high-resolution continuum source electrothermal atomic absorption spectroscopy (HR-CS ETAAS). For this purpose, a simple approach for the extraction of Pd and Rh from auto catalysts with aqua regia (HCl + HNO3 (3:1)) leaching was developed. The optimum conditions for leaching of both elements in an open system are 4 h, at 108 °C and a solid-to-liquid ratio of 1:40. Palladium and rhodium concentrations in obtained leachate were determined simultaneously in one firing using the less sensitive adjacent secondary lines for Pd at 360.955 nm (7.7% relative sensitivity) and for Rh at 361.251 nm (2.2% relative sensitivity). The pyrolysis and atomization temperatures were 1200 °C and 2600 °C, respectively. Under these conditions, the limits of detection for Pd (0.19 mg kg−1) and Rh (0.48 mg kg−1) were relatively high but the sensitivity of the developed method corresponds to common concentrations of the elements in spent automotive catalysts. The repeatability of the method expressed as a relative standard deviation was typically 10% for Pd and 7% for Rh.The trueness of the method was confirmed by analysis of the certified reference material – spent automobile catalyst (ERM EB 504). The recoveries of Pd and Rh from the spent automobile catalyst were 96.9%, and 87.9%, respectively. The method was successfully applied to the simultaneous determination of Pd and Rh in spent automotive catalysts without preliminary chemical separation.

A closed-loop integrated process of bipolar membrane electrodialysis and resin adsorption for resource recovery from high-salinity phenolic wastewaters: Salicylic acid manufacturing wastewater as a representative

Phenolic compounds stand as consequential chemical precursors, boasting an extensive array of industrial applications. The worldwide yield of such compounds attained 17 million tons annually, and a substantial volume of effluent characterized by elevated phenolics content along with inorganic salinity was emanated from the manufacturing processes, which would exert profound deleterious impacts upon the ecosystem and aquatic environment. Scant literature was available regarding efficacious treatment of these high-salinity phenolic wastewaters and complete recovery of its constituents currently. In this study, initially, various high-salinity phenolic wastewaters were treated in bipolar membrane electrodialysis (BMED) system, and the migration behavior of constituents was investigated. Drawing upon the migration patterns and the efficacy of BMED, a closed-loop integrated process of BMED and resin adsorption was proposed to thoroughly address this kind of wastewater and attain comprehensive material reclamation. Because of the wide use of salicylic acid (SA) all over the world, the enormous production in China, and the complexity of the sewage which contained rich Na2SO4, phenol, and SA from manufacturing process, SA manufacturing wastewater was selected as the representative sample. For the BMED process, the desalination rate got 99.4 % under the optimum conditions. The concentrations of H2SO4 and NaOH obtained were 0.56 and 0.78 mol/L. The preliminary separation of phenol and SA was realized during desalination. Then, the effluent of BMED feed compartment was injected into two-stage resin unit. The satisfactory regeneration of both spent resins was achieved, and high-purity phenol and SA were obtained. No contaminants were undetected in the effluent, allowing for the reuse of the effluent within BMED. The complete resource utilization of SA manufacturing wastewater was realized, indicating the viability of proposed integrated process for treating and resource recovering of analogous high-salinity industrial phenolic wastewater.

Effect of a vortex plate on turbulent flow within an axial hydrocyclone for preliminary water separation

Effect of a vortex plate on turbulent flow within an axial hydrocyclone for preliminary water separation

Efficiency of preliminary gravity separation of gold-bearing ore from the Malinovka deposit

Efficiency of preliminary gravity separation of gold-bearing ore from the Malinovka deposit

Effect of a vortex plate on turbulent flow within an axial hydrocyclone for preliminary water separation

Effect of a vortex plate on turbulent flow within an axial hydrocyclone for preliminary water separation

Extracting photometric determination of antimony with 5–pyridylazo–2–monoethylaminoparacresol (PAAC)

The developed extraction-spectrophotometric method for the determination of antimony utilizing 5-(pyridylazo)-2-monoethylaminoparacresol (PAAC) stands out for its selectivity, sensitivity, and rapidity. This method proves to be effective in the determination of antimony in various complex matrices, including model solutions of silicate rocks and ores. The robustness of the method is evident as it successfully analyzes these samples without the need for preliminary separation of accompanying elements. PAAC is chosen as the complexing agent for antimony. Its selectivity and sensitivity contribute to the precision of the method. The method involves an extraction step, likely utilizing a suitable organic solvent, to selectively extract the antimony-PAAC complex from the sample matrix. The concentration of antimony in the extracted complex is determined spectrophotometrically. This involves measuring the absorbance of the complex at a specific wavelength, typically chosen based on the characteristics of the PAAC-antimony complex. The method is validated by analyzing model solutions of silicate rocks and ores. This step assesses the applicability and accuracy of the method in samples that simulate real-world conditions. The method demonstrates a reproducibility of 2-5%, indicating its reliability and precision across multiple analyses. The developed method is recommended for the analysis of chemically complex materials such as industrial wastewater, ores, concentrates, and rocks.

Роторный сепаратор очесанного зернового вороха в наклонной камере комбайна

Preliminary separation minimizes damage of free grain by the working parts of the thresher when stripping standing crops. For this purpose, it is advisable to use the inclined chamber of a combine harvester. The purpose of this study is to evaluate the separating ability of a rotary separator depending on its design parameters and operating mode. The research included two stages. In the first series of experiments, the distance between the rod rings was changed (6; 8; 10; 12, and 14 mm), in the second series: the peripheral speed of the rotor surface (1 to 3 m/s). Experimental studies were carried out on winter wheat of the “Moskovskaya 56” variety with a moisture content of 12%. The inclination angle of the experimental installation body in all variants of the experiment was 45°, and the supply of stripped grain heap was 1 kg/s. Based on the results of laboratory studies, it was established that the intensity of separation of free grain increases as the distance between the rod rings of the device increases and the peripheral speed of the rotors decreases. The maximum passage of loose grain (100%) corresponds to a peripheral speed of the rotors of 1 m/s and a distance between the rod rings of 14 mm. The reserves for increasing the productivity of a rotary separator are an increase in the diameter of the rotors and a decrease in their peripheral speed. When these parameters change by 30 to 35%, the transport capacity of the inclined chamber remains at an acceptable level.

Addendum to the Technology for Extraction of Scandium From Matte of the Kadamzhai Antimony Plant

The article discusses methods for extracting rare earth elements from the waste (matte) of the Kadamzhai antimony plant. For this purpose, the quantitative determination of scandium was used by photocolorimetry of solutions of its complexes, which, compared to the known ones, will allow analysis within 25-30 minutes with sufficient accuracy and makes it possible to determine scandium in technogenic waste without preliminary separation of interfering impurities. To do this, add ascorbic acid to the solution, adjust the pH to 1-2, then add xylenol orange and colorimeter using the usual methods Photocolorimetry is not affected by alkaline earth elements (Ca, Ba, Sr, Mg), rare earth elements (La, Pr, Nd, Sm and others), as well as yttrium, zinc, cadmium, manganese, iron. The harmful effects of Fe are eliminated by the addition of ascorbic acid.

Fluorimetric study on a novel FDA-approved combination used for the treatment of overactive bladder syndrome in different matrices

For the first time, two spectrofluorimetric techniques were created for the concurrent determination of mirabegron (MBN) and solifenacin (SOL) in different matrices. This novel combination recently received FDA approval to treat overactive bladder syndrome and was marketed under the brand name MiragoTMS25. SOL has a native fluorescence and can be estimated directly in the presence of MBN without any interference at 290 nm after excitation at 230 nm. In contrast to SOL, MBN is a weak fluorescent substance that requires the use of a fluorogenic reagent (like NBD-Cl) to be measured simultaneously with SOL in their mixtures. So, we reacted MBN with NBD-Cl to yield a yellow-colored fluorophore that could be estimated at 546 nm after excitation at 471 nm without any preliminary separation or interference from SOL. All experimental conditions of the applied methods were tested and enhanced for the best results. The applied fluorimetric methods succeeded in determining both drugs in nanograms, making them applicable in biological fluids like human plasma. The linearity ranges of SOL and MBN were found to be 5–250 ng/mL and 50–600 ng/mL, respectively. The LOD values were 0.96 ng/mL and 5.09 ng/mL for SOL and MBN, in order. Excellent results were attained for both drugs during their analysis in spiked plasma samples employing the applied fluorimetric methods, confirming their suitability for use in real human plasma samples and paving the way for further bioequivalence studies on both drugs.

Uptake of plutonium isotopes by green onions in different growing mediums

During the growing experiment, the uptake of plutonium isotopes by green onions (Allium cepa Centurion F1) grown on distilled water, Ellis nutrient solution and sod-podzolic soil was studied. The analysis of plutonium isotopes was carried out by alpha spectrometry with preliminary radiochemical separation. It has been determined that the uptake of 239+240Pu by the green onions decreases in a row: distilled water > Ellis nutrient solution ≫ sod-podzolic soil. The transfer factors of plutonium isotopes in green onions were 1.7 ± 0.4 for distilled water; 0.7 ± 0.2 for Ellis nutrient solution; 0.0054 ± 0.0014 for soddy-podzolic soil.

Smart Multivariate Spectrophotometric Determination of Two Co-Administered Autoimmune Drugs; Sulfasalazine and Pentoxifylline; in Bulk and Spiked Human Plasma.

Sulfasalazine and pentoxifylline are co-prescribed together to treat psoriasis and pemphigus vulgaris. Sulfasalazine is an anti-inflammatory, immunosuppressant, and antibiotic drug, while pentoxifylline is a vasodilator and immunosuppressant. The spectra of the two drugs and plasma suffer from severe overlap. This work aims to simultaneously determine sulfasalazine and pentoxifylline in their binary mixture and spiked human plasma by the assessment of their UV spectral data. Two model updated chemometric methods were established using principal component regression and partial least-squares regression models. The two models were validated in accordance with the U.S. Food and Drug Administration guidelines for bioanalysis and were applied for the determination of both drugs in synthetic mixtures or spiked human plasma. Accuracy and precision were within the accepted limits. In addition, three different assessment methods were used to evaluate the environmental greenness of the proposed models. The two updated models are simple, rapid, sensitive, and precise, and could be easily applied in QC laboratories for determination of sulfasalazine and pentoxifylline, without any preliminary separation steps or interference from plasma matrix. Two updated chemometric models called principlal component regression and partial least-squares regression were established for determination of sulfasalazine and pentoxifylline in spiked human plasma using UV spectrophotometric data.

Analysis of nine nitrosamines relevant to occupational safety by ion mobility spectroscopy and preliminary gas chromatographic separation

Nitrosamines have been identified as a probable human carcinogen and thus are of high concern in many manufacturing industries and various matrices (for example pharmaceutical, cosmetic and food products, workplace air or potable- and wastewater). This study aims to analyse nine nitrosamines relevant in the field of occupational safety using a gas chromatography-drift tube ion mobility spectrometry (GC-DT-IMS) system. To do this, single nitrosamine standards as well as a standard mix, each at 0.1 g/L, were introduced via liquid injection. A GC-DT-IMS method capable of separating the nitrosamine signals according to retention time (first dimension) and drift time (second dimension) in 10 min was developed. The system shows excellent selectivity as each nitrosamine gives two signals pertaining to monomer and dimer in the second dimension. For the first time, reduced ion mobility values for nitrosamines were determined, ranging from 1.18 to 2.03 cm2s−1V−1. The high selectivity of the GC-DT-IMS method could provide a definite advantage for monitoring nitrosamines in different manufacturing industries and consumer products.

The improvement of operational properties of the parts of means of transport by applying regular reliefs

Problem. Specially created regular surface reliefs strongly influence the reliability and operational properties of machine parts. One of the effective methods of obtaining reliefs is cold plastic deformation (CPD). This method of modification does not lead to the occurrence of significant stresses in the material of the part and can be form-forming. The latter is important in repair production. However, to date, there is no systematic study of the formation of reliefs by CPD methods, especially with the use of linear indenters. Goal. To improve the operational properties of the parts of means of transport by applying regular micro- and macro-reliefs of technological and operational purposes by deepening linear indenters. Methodology. Press hydroficated equipment was used to create a normal indenter penetration force. The brands of typical structural materials are defined: steel, gray cast iron, bronze, brass, aluminum and titanium alloys. A linear indenter made of high-speed steel with angles at the top of 60° - 90° and a radius at the base of 0.005 - 0.5 mm was created. Methods of microstructural analysis and microhardness, standard and original instruments and devices were used. The range of groove depths was 0.02-0.5 mm. A lubricating-cooling liquid based on rapeseed oil was used. Results. It was hypothesized that when the indenter is immersed in the material of the part, the factors that have the greatest influence on the depth and other parameters of the relief groove are the normal strength, hardness, and relative elongation of the material under study. These theoretical principles formed the basis of the mathematical model of the indentation process. Originality. It was created a classification of regular reliefs by purpose: for preliminary division of the allowance during cutting; for preparing the surfaces of parts for applying coatings, including anti-friction films; to create labyrinths on the surface of parts for lubricants; for preliminary separation of fragmentary ammunition cases; to create strengthened new load-bearing surfaces, in particular, discrete when restoring worn parts; to obtain complex profiles on the surfaces of the holes of the parts. The calculation of the impact of the indentation force and the geometric parameters of the linear indenter on the shape and dimensions of the relief groove has been developed. Theoretical propositions about the influence of the embedment force, hardness and plasticity of the material of the part on the parameters of the relief groove were experimentally confirmed. These factors affect the optimization parameter both directly and through intermediate factors: the radius of rounding and the angle at the top of the indenter and the composition of the lubricating and cooling liquid and the speed of immersion. The interrelationship of phenomena during the formation of reliefs and the dependence of the depth of the groove on the process factors are constructed. Practical value. The method of restoring worn parts by indenting a linear indenter was developed, the principle of obtaining discrete surfaces of machine parts by alternating relief grooves with flat areas within a discreteness of 5-50% and methods of obtaining shaped surfaces in the holes of hollow parts by copying were developed.

A sustainable approach for the degradation kinetics study and stability assay of the SARS-CoV-2 oral antiviral drug Molnupiravir

Molnupiravir (MPV) is the first direct-acting oral antiviral drug that effectively decreases nasopharyngeal infections with SARS-CoV-2 virus. The stability of MPV was tested by subjecting the drug to various stress conditions. The drug is liable to oxidative, acidic, and alkaline degradation and showed significant stability against thermal degradation. Mass spectrometry identified the degradation products and guided suggestion of the degradation patterns. Interestingly, while inspecting the UV-absorption spectra, we observed no absorbance at 270 nm for the products of the three degradation pathways (c.f. intact MPV). Direct spectrophotometry seemed a solution that perfectly fit the purpose of the stability assay method in our case. It avoids sophisticated instrumentation and complex mathematical data manipulation. The method determined MPV accurately (100.32% ± 1.62) and selectively (99.49% ± 1.63) within the linear range of 1.50 × 10–5–4.0 × 10–4 M and down to a detection limit of 0.48 × 10–5 M. The proposed method is simple and does not require any preliminary separation or derivatization steps. The procedure proved its validity as per the ICH recommendations. The specificity was assessed in the presence of up to 90% degradation products. The study evaluated the greenness profile of the proposed analytical procedure using the National Environmental Methods Index (NEMI), the Analytical Eco-Scale, and the Green Analytical Procedure Index (GAPI). The three metrics unanimously agreed that the developed procedure results in a greener profile than the reported method. The method investigated the degradation reactions' kinetics and evaluated the reaction order, rate constant, and half-life time for each degradation process.

A rapid pesticide detection approach in food forensics using hyphenated technology of TLC-electronic nose

Pesticides are a commonly employed means for improving agri-yield but have grave forensic implications in cases of accidental or deliberate pesticide poisoning. There is a need of devising a rapid, portable, and accurate in-situ technique to detect pesticides in food samples encountered at the scene of crime. The technology Electronic Nose (E-nose) has the potential to detect pesticides rapidly by identifying the volatile constituent compounds in tested samples and generating corresponding "smell prints." The aim of this study was to determine a combined multiclass/multiresidue analytical approach using Thin Layer Chromatography (TLC) and E-nose to be employed as a reliable, rapid and cost-effective method for detecting pesticides in different food samples at crime scene in cases of pesticide poisoning. Five food matrices were spiked with eight pesticides of standard concentration. TLC was used for a preliminary separation of pesticides in each of the food matrices spiked with the eight pesticides individually. Further, E-nose was employed to build a prediction model using two specific pesticides, atrazine and diafenthiuron. The data obtained was analyzed using built-in principal component analysis (PCA) and the spiked samples showed congruence to the prediction model. The results demonstrate the E-nose’s efficiency of detecting the presence of pesticide-contaminated food matrices as it responded well to the volatile pesticide components with good linearity (R2>9.998) as per the developed protocol. We advocate this combined approach to be utilized for on-field rapid and reliable qualitative forensic testing for pesticide contamination in food matrices with minimal sample preparation.

Application of diabatic extractive distillation schemes with preliminary separation of azeotropic components for separation of acetone-toluene-<i>n</i>-butanol mixture

Objectives. The study aims to investigate the effectiveness of the use of various options for organizing the process of diabatic distillation in the separation of a mixture of acetone-toluene-n-butanol by extractive distillation using dimethylformamide as an entrainer in a scheme with preliminary separation of azeotropic components.Methods. As the main research method, mathematical modeling in the Aspen Plus V. 12 software package was used. As a model for describing vapor-liquid equilibrium, the local composition Non-Random Two Liquid equation model was used. Parametric optimization of diabatic schemes was carried out according to the criterion of reduced energy costs.Results. Based on the scheme for extractive distillation of an acetone-toluene-n-butanol mixture with preliminary separation of azeotropic components, five options for organizing diabatic distillation schemes were considered, both with and without use of a compressor to reach a required flows temperature.Conclusion. It is shown that the use of diabatic schemes in the extractive distillation of a acetone-toluene-n-butanol mixture with dimethylformamide makes it possible to diminish the reduced energy costs by 8.9-43.5%. Meanwhile the maximum reduction in energy consumption is achieved in a scheme where upper vapor flows of two other columns are used to heat the azeotropic components separating column.

Exploring the anti‐α‐amylase activity of flavonoid aglycones in fabaceae plant extracts: a combined MALDI‐TOF‐MS and LC–MS/MS approach

SummaryA combination of TLC‐bioautography, MALDI‐TOF‐MS and LC–MS/MS methods was used to identify flavonoids with anti‐α‐amylase activity in extracts of Lathyrus pratensis L. (herb), L. polyphillus L. (fruits), Thermopsis lanceolata R. Br. (herb) and S. japonica L. (buds). After the TLC‐autobiography assay, substances with anti‐amylase activity were identified by MALDI‐TOF‐MS followed by confirmation of the result by LC–MS/MS. Results of the study revealed that the flavonoids apigenin, luteolin, formononetin, genistein and kaempferol display marked anti‐α‐amylase activity. Formononetin showed the largest activity. Compared with LC–MS/MS, MALDI‐TOF‐MS is a quick and convenient method; results can be obtained within minutes; and only minor sample amounts are required which allows us to analyse mixtures of substances without preliminary separation. However, the inability to distinguish between isomers is the main limitation of the method.

Influence of Cellulose on the Anoxic Treatment of Domestic Wastewater in Septic Tanks: Statistical Analysis of the Chemical and Physico-Chemical Parameters

Cellulose is a very common polymer in domestic wastewater (WW), representing a not negligible part of the organic substance contained in sewage. To date, many studies have highlighted the feasibility of reusing this compound in several ways (e.g., building sector, wastewater treatment, energy production, etc.) after its separation from domestic WW. However, studies about the impact of the absence of cellulose on the chemical and physico-chemical parameters of a biological process are still lacking. In this work, two pilot-scale plants were used to simulate an anoxic treatment of WW in septic tanks, with and without cellulose (CWW and NCWW, respectively), for three months. The results of the monitoring highlighted that T, pH, and electrical conductivity (EC) remained almost constants, in both cases. The Spearman correlation coefficients (SCC) for turbidity (TUR), total suspended solids (TSS), and color (COL) indicated a higher removal in the case of CWW (65%, 66%, and 56%, respectively). Organic substance and nitrogen forms showed a similar behavior with and without cellulose, but in the case of CWW, N-NH3 was highly negatively correlated with TUR (SCC: −0.54), TSS (−0.49), and COL (−0.39). A biological denitrification process was highlighted in both cases. Despite these differences, when statistically analyzing the trends of the chemical and physico-chemical parameters for CWW and NCWW, a significant difference due to the absence of cellulose was excluded. These results will be useful to the scientific community, as they exclude that the operational parameters of anoxic treatments and the effectiveness on pollutants removal can be affected in the case of preliminary cellulose separation from domestic WW for recovery/reuse purposes.