Preliminary Phytochemical Studies Research Articles

Versatile silver nanoparticle using Tradescantia spathacea leaf extract: Synthesis, physiochemical, in vitro biological activity and photocatalytic evaluations

This study portrays the preparation of silver nanoparticles (AgNPs) using the leaf extract of Tradescantia spathacea (TS) through a green synthetic protocol. The green TS-AgNPs synthesized were physiochemically evaluated for its UV–Vis absorbance, IR vibrational behavior, powder diffraction nature, size and shape by HR-TEM procedures. The surface plasmon resonance (SPR) spectra of the TS-AgNPs exhibit a SPR peak maximum at 415 nm; powder XRD analysis proved the purity and crystallinity of the synthesized TS-AgNPs. Particle size resulting from HR-TEM discovered numerous spherical particles with 18 nm average size, consistent with the XRD analysis. Presence of metabolites such as alcohols, phenols,coumarins, phytols, phytosterols, tannins, glycosides, and proteins were identified with preliminary phytochemical and FTIR studies. In vitro antimicrobial evaluation demonstrated that the resulted TS-AgNPs exhibited an outstanding antimicrobial activity against both Gram-negative and Gram-positive bacteria in a dose-dependent manner. Additionally, the nanoparticles showed dose-dependent anti-biofilm activity (89.45 ± 3.56 % activity at 200 μg/mL), DPPH scavenging activity (96.5 ± 3.1 % at 200 μg/mL with an IC50 value of 43.62), and inhibition of heat-induced albumin denaturation (in vitro anti-inflammatory activity) with 61.52 ± 2.25 % at 400 µg/mL, comparable to the diclofenac standard (94.78 % at 400 μg/mL). Furthermore, the TS-AgNPs demonstrated an excellent blood compatibility, cytotoxicity against MCF-7 cancer cell lines and exhibits photocatalytic activity in degrading Congo red dye (96.19 % degradation was achieved).

Phytochemical Characterization of Hylocereus polyrhizus Rind Extracts

The purpose of this study was to identify the phytochemical components of rind extracts from Hylocereus polyrhizus. Two distinct solvents were used in the maceration process to create the extracts. Following a preliminary phytochemical study of the extracts, the principal bioactive substances, including tannins, flavonoids, and alkaloids, were quantitatively identified. Using LC-MS and HPTLC, the H. polyrhizus rind extracts are phytochemically characterized. Chemical profiling of the extracts, retention factor of the separated bands, and constituent percentage area were all determined using HPTLC. By using LC-MS, about 28 phytochemical components and compounds were examined. A sizable concentration of bioactive chemicals in H. polyrhizus suggests that the plant has pharmacological properties that include neurological, anticancer, antidiabetic, antioxidant, and cardioprotective properties.

Phytochemical Screening and Evaluation of Antimicrobial Activity of Desmostachya bipinnata Roots

This study was conducted for preliminary phytochemical study of different extracts and to study antimicrobial effects of methanolic extract of Desmostachya bipinnata roots. Desmostachya bipinnata Stapf is an official drug of ayurvedic pharmacopoeia, belongs to the family of Poaceae/Gramineae. Phytochemical screening was done to identify the main groups of chemical constituents present in different extract of Desmostachya bipinnata by their colour reactions with different reagents. Four extracts viz: Petroleum, chloroform, ethyl acetate and methanolic, were subjected for carbohydrates, flavonoids, glycosides, alkaloids, saponins, proteins and amino acids using a test procedure. Preliminary antimicrobial test of D. bipinnata extract was carried out by well diffusion method. The extract was subjected for antimicrobial screening using reference standard, Gentamicin (10µg). The antimicrobial screening of the plant extract revealed the antimicrobial activity against Staphylococcus aureus (Gram positive microorganism) and Escherichia coli (Gram negative microorganism).

Investigation of in-vitro antioxidant activity and heavy metals present in Ficus mollis leaves

The folklore medicinal plant leaves of Ficus mollis were selected for the study. We were performed for elemental analysis, i.e., macronutrients and toxic and heavy metal contents. All the values are within the safe limits. The plant specimens were successively extracted with solvents such as petroleum ether, Chloroform, ethyl acetate, and Ethanol. The preliminary phytochemical studies of FML confirmed the presence of flavonoids, tannins, steroids, glycosides, alkaloids, and triterpenoids. The present study also investigated FML antioxidant activity using in vitro methods. The EEFML showed significant antioxidant activity in all models studied compared with ascorbic acid. It possesses good activity in scavenging DPPH radical, Hydroxyl radical, Nitric oxide radical, and scavenging H2O2.Further investigation of individual Phyto compounds, their isolation, identification, and their role as free radical scavengers is needed.

Pharmacognostical Studies on 'Jivanti' Part IV - <I>Dregea Volubilis</I> (Linn. F.) Benth

'Jivanti an important Ayurvedic drug, has variously been ascribed to different botanical taxa, Dregea volubilis (Linn.f.) Benth. being one such species. A detailed pharmacognosy of vegetative parts of the above species has been dealt within the present paper. This includes organoleptic and microscopic characters, fluorescence analysis of the drug powder and its behaviour on treating with different chemical reagents, preliminary phytochemical studies of various extractives and their TLG patterns and ash values. Some of the salient diagnostic characters revealed by this study are : diarch primary stele in the root, origin of phellogen in the second cortical layer of stem, presence of pericycle as 2 rows of irregularly arranged strands of partially lignified fibres mixed with a few, sclereids, commonly occurring uni- and bi-seriate rays in both the root and the stem, a stout obscurely channelled petiole with three leaf traces, development of red colour in the aqueous extract of powdered root on heating with phloroglucinol and conc. Hcl.

Phytochemical investigation of Nigella sativa seed extract by HPTLC, HPLC and GC-MS: a comparative geographical study

This study aimed to ensure the quality of the seed as well as determine the phytochemical composition of Nigella sativa seed extract (NSSE) obtained from three different geographical locations. Pharmacognostic evaluation of the seed includes preliminary phytochemical screening, physicochemical evaluation, and study of heavy metal content, in addition to HPTLC, HPLC, and GC-MS studies of the extract obtained from the seed of the Nigella sativa (NS). HPTLC fingerprinting studies revealed the presence of various bioactive compounds. HPLC analysis confirms the quantitative variation of thymoquinone (TQ) in the extracts, i.e. the maximum quantity of TQ was found in Vizag NSSE, followed by Punjab and Madhya Pradesh. GC-MS analysis reveals the presence of 33, 35, and 32 constituents in the extract obtained from Vizag, Madhya Pradesh, and Punjab, respectively. This study confirms the variation in the phytochemical composition as well as in the biomarker (Thymoquinone) content present in the collected samples.

Standardization and Phytochemical screening of Abutilon crispum

The main objective of the research is to recognize, validate and also to avoid the adulteration of plant Abutilon crispum. The correct identification of the plant Abutilon crispum was possible because of preliminary phytochemical screening, extraction by maceration and standardization. Preliminary phytochemical screening and TLC studies were carried out by standard methods with extracts such as chloroform, ethyl acetate and methanol. While standardization comprises the evaluation of parameters like pH range, water-soluble ash, loss on drying, total ash, acid insoluble ash, sulfated ash, ethanol-extractable parameters. The following values are found to be the results of the above testsi.e, loss on drying and total ash values are 7.2±0.22 and 6.32±0.04 correspondingly, water soluble ash was 1.33±0.05. Preliminary phytochemical evaluation for these extracts discovered the occurrence of flavonoids, steroids, terpenoids, glycosides and alkaloids in this plant. In methanol extract also major components such as alkaloids, flavonoids, tannins and glycosides were found. The thin layer chromatography examination of Abutilon crispum displayed the occurrence of steroids with0.76 and 0.52 Rf values in diverse solvent systems. TLC investigation of extract of ethyl acetate exhibited Rf values 0.74 and 0.81 due to occurrence of flavonoids with in dissimilar solvent systems similarly. The evidence found from contemporary study deliver valuable data that will be supportive in identifying and to perform upcoming research in future.

Bioactive potentialities of Grewia flavescens root extract in the treatment of alcohol dependence: Preliminary phytochemical study

Although the use of plants for medicinal purposes is an ancient practice, there is a perception that in the field of chemical dependency, its role has been and is little studied. With regard to alcohol dependence, the third leading cause of hospitalization in psychiatric hospitals in Mozambique, an ethnobotanical work was carried out in 2018 in the Municipality of Massinga, province of Inhambane, with the aim of identifying with practitioners of traditional medicine, the plants they use in their treatment, having resulted, among other plants, in the identification of Grewia flavescens, from which the need arose, based on phytochemical prospecting and literary framework support, to carry out a study of a preliminary nature on the bioactive potential of the root extract (part indicated in the survey) of Grewia flavescens in the treatment of alcohol dependence. The results showed the presence of Flavonoids, Tannins, Alkaloids, Triterpenoids and Saponins. About this group of bioactives, there is bibliographical evidence that points to the anxiolytic and anti-depressant action of Flavonoids, Saponins and Alkaloids, allowing to infer that these can act as mood stabilizers in patients in the phase of alcohol withdrawal syndrome and craving during treatment, not despite deepening from preclinical studies.

Ziziphus rugosa Leaf: Pharmacognostical Characters and Anti-Inflammatory Properties against Carrageenan-Induced Paw Edema

Ziziphus rugosa belongs to the family Rhamnaceae, which includes many flowering species, primarily trees and shrubs, and sometimes vines. This study aims to describe the pharmacognostic characteristics and potential anti-inflammatory properties of Z. rugosa leaf. The pharmacognostical and preliminary phytochemical studies were performed following standard procedures. Acetone, ethanol, and aqueous extracts were screened for anti-inflammatory potential using the carrageenan-induced paw edema model. Ziziphus rugosa was identified by its evergreen nature, recurved hooks, and drupe-type fruits. Leaves are elliptic/rounded with cordate base exhibiting a dark green glossy upper surface and pubescent lower surface. The leaf exhibited a dorsiventral nature in the transverse section, covering trichomes, collenchyma, sclerenchyma patch, and calcium oxalate crystals as key histological characters. Anamocytic stomata, covering trichomes, crystals, and fragments of vessels, are the imperative elements in powder. The extracts contain carbohydrates, alkaloids, glycosides, tannins, saponins, phenolic compounds, proteins, and flavonoids. The acetone extract at 400 and 200 mg/kg displays a maximum inflammation inhibition of 56.96% and 48.77% among the extracts, and the standard diclofenac sodium inhibits inflammation by 65.61% at 24 hours. The altered liver superoxide dismutase, glutathione, and malondialdehyde levels in the positive control group are significantly near normal in the treatment groups. The histopathological studies of treated animals show significant protection against paw and liver tissue damage. Pharmacognostical study outcomes aid in the identification of species along with ascertaining standardization parameters. Further fractionation of acetone extract followed by isolating compounds responsible for the anti-inflammatory activity would provide an alternative to managing inflammation.

Preparation and Evaluation of Classical Ayurvedic Formulation Triphala gritha

Objective The objective of this study was to prepare and evaluate Triphala gritha.Methods The selected formulation Triphala ghrita was prepared as per the methodology described in Ayurvedic texts. The prepared and marketed formulations were analyzed for their physico-chemical constants using established methods in accordance with WHO guidelines the Indian Pharmacopoeia and standard protocols from the Ayurvedic Pharmacopoeia of India Herbal Pharmacopoeia and other relevant sources.Results A profile consisting of distinguishing parameters was developed to provide means of correct identification. Some important organoleptic features of Triphala ghrita laboratory and market formulations like color odour taste appearance touch etc. were documented. Physico-chemical constants were determined and documented. The formulations were tested for the presence of heavy metals microbial content pesticide residues and aflatoxin which were also found within the limits of WHO specifications. Preliminary phytochemical studies of Triphala ghrita revealed the presence of terpenoids and phenolics as chief constituents. High performance thin-layer chromatography HPTLC method was developed and reported for the quantification of ascorbic acid and gallic acid as marker constituents to ensure identity and quality of Triphala ghrita.Conclusion The studies carried out on Triphala ghrita can serve as a valuable tool and provide suitable standards for its identification. Phytochemical screening and the quantitative HPTLC assay method developed for quantification of ascorbic acid and gallic acid in Triphala ghrita can serve for the routine quality control of commercial samples and help in justifying the traditional claims endowed upon this formulation in a scientifically accepted manner.

Phytochemical Composition, Antioxidant and Antibacterial Studies on Celtis Timorensis Leaf Extract

Objective: The objective of the present investigation is to study the phytochemical composition, antioxidant and antibacterial activity of petroleum ether, chloroform, ethanol, methanol and water extracts of the leaf part of Celtis timorensis Spr. The preliminary phytochemical studies were conducted according to standard procedures. Total phenolic content was estimated using the FCA reagent method. The antioxidant efficiency of extracts was evaluated by using molybdate and DPPH methods. The antibacterial potency of leaf extracts was studied using the disc diffusion method against eight human pathogenic bacterial strains. Results: The results of preliminary phytochemical study revealed the presence of alkaloids, phytosterols, phenolic components, tannins, flavonoids, terpenoids, glycosides and saponins. The total phenolic content of the tested extracts exhibited a range between 8.82 to 68.32 mg GAE/g dwt. The highest total phenolic content was observed in the methanol extract (68.32±1.03 mg GAE/g dwt.) and the highest total antioxidant capacity was observed in the methanol extract of leaf part (700.0±0.71 mg ASE/g dwt.).Regarding DPPH scavenging activity the highest DPPH-reducing activity (>90%) was observed by methanol, ethanol and water extracts of the leaf part. Ethanol and water extracts of leaf samples strongly inhibited the gram-negative bacterial species Pseudomonas aeruginosa and Salmonella enterica (13 mm for each species) respectively. While gram-positive species i.e. Bacillus megatherium Artherobacter protophormiae and P. aeruginosa were moderately inhibited by chloroform, ethanol and water extracts (12 mm for each) respectively. Conclusion: In conclusion, the selected medicinal plant C. timorensis extracts exhibited good antioxidant activity, strong antibacterial activity and rich bioactive components. It required further studies on the isolation, and characterization of active principle to evaluate its pharmacological properties.

A Comparative Analytical Study of Krishna Musali Choorna and Krishna Musali Kshara

Krishna Musali (Curculigo orchioides) is among the Dashapushpa, a group of ten herbs which is culturally and medically important to the people of Southern India, especially in Kerala. Krishna Musali is mentioned in various context in the names of Krishna Musali, Talapatri, Talamooli etc. and it is having Madhuratikta rasa, Ushna virya and it is Vatapittahara and Rasayana properties. In classics references about this drug can be seen in the context of Dhoomapana yoga, Kshara vidhi, Ekangasopha chikitisa etc and the Acharyas were categorized in to specific Gana or Varga based on its mode of action. Krishna Musali Choorna and Kshara prepared based on the method of preparation mentioned in Sharangadhara Samhita. Analytical study was carried out for the purpose of standardization. For the qualitative assurance of the raw material analytical study provides the objective parameters for the standardization leading to a reproducible quality. These tests were done for evaluating the anticancer activity of Krishna Musali Choorna and Kshara in cervical cancer cell lines. The preliminary phytochemical study shows high alkalinity of Krishna Musali Kshara compared to Choorna, it supports the definition of alkali of being caustic and corrosive in nature. So, it will be helpful in the treatment of early stages of Cervical carcinoma.

Evaluation of Substitution of Stem Bark with Small Branches of Stereospermum Chelonoides DC and Quantitative Estimation of Lapachol by HPLC

Background: Stereospermum chelonoides DC, also known as padhri, is a medicinal plant that belongs to the Bignoniaceous family and is commonly used in herbal medicine. Natural materials separated from a variety of traditional herbal plants have been claimed to had a significant impact on the development of numerous lead compounds used in the treatment of health issues in communities all over the world. The plant stem bark and small branches is used in traditional and folklore medicine to treat a variety of ailments. The aim of this study is to assess the Pharmacognostic and physicochemical analysis of Stereospermum chelonoides stem bark and small branches. The stem is used for the study of characters; whereas physicochemical parameters such as pH, total ash content, acid insoluble ash, water soluble extractive, alcohol soluble extractive, loss on drying are determined. Results: Preliminary phytochemical studies of ethanolic and aqueous extract of Stereospermum chelonoides DC ascertained the presence of flavonoids, saponins, Coumarins, Alkaloids, Furanoids, phenolic, Quinones, Reducing sugars, Sugars (Carbohydrate), triterpenoids, and tannins. The findings of the present study lay down standards which could be useful to detect the authenticity and for detailed evaluation and investigation of this plant. Lapachol which is indicated in the High Performance Liquid Chromatography, further studies may be conducted on the method validation by High Performance Liquid Chromatography to quantify in formulations, extracts and raw materials for quality control purpose. Conclusion: The stem bark and small branches of this plant useful in the future as it will not have any harmful effect to the plant so its small branches are more important than stem bark in the formulation of drugs which will derive from Stereospermum chelonoides DC

Pharmacognostic Evaluation and In-vitro Evaluation of Antioxidant activity of Mesua ferrea Linn

India is the great heritage of medicinal herb and aromatic plants. Mesua ferrea is a well known plant for its medicinal property in Ayurveda and other traditional system of medicine. It possess multifactorial pharmacological effect because of its rich source of secondary metabolites. According to phytochemical studies, M. ferrea is a good source of polyphenolic, flavonoids contents that gives significant antioxidant property. The aim of this research work was to perform detailed pharmacognostic study like micsoscopy, physicochemical parameters, preliminary phytochemical screening, and determination total phenolic content as well as antioxidant activity of alcoholic, hydro alcoholic and water extracts of M. ferrea. Preliminary phytochemical study showed the presence of sterols only in alcoholic extract, and flavonoids, phenols, coumarins, and saponins, glycosieds in both alcoholic and water extract. Hydroalcoholic extract contain highest total phenolic content, and showed antioxidant activity to the greater extent than the alcoholic and water extract. In vitro study revealed the remarkable antioxidant effect and highest phenolic content in hydroalcoholic extract.

Callus Culture of <i>Aralia cordata</i> Thunb.: Obtaining, Selection of Cultivating Conditions, Somatic Embryogenesis Induction

Introduction. Cell cultures spreads widely in different areas of biology, biotechnology and agriculture. Aralia cordata is a perennial herbaceous plant, which has been listed in Red book of the Russian Federation. Pharmaceuticals which are based on raw materials of Aralia ssp. have valuable types of pharmacological activity and are widely used in oriental medicine. A serious obstacle for resumption of the natural populations is the presence of the period of morphophysiological dormancy in the seeds of the plants, which requires a long-time stratification process. Limitation of the natural geographic range and the combination of biological activities useful for humans make Aralia cordata Thunb. prospective object for in vitro introduction.Aim. The aim of the study is obtaining of the viable cell culture of Aralia cordata Thunb., investigation of the somatic embryogenesis conditions.Materials and methods. Pieces of the leaves of Aralia cordata intact plant from Komarov Botanical Institute of the Russian Academy of Sciences were used as a primary explants. Pieces of leaves were sterilized in a 2 % benzalkonium chloride solution for 5 minutes, induction of primary callogenesis was carried out on Murasige – Skoog medium. Nutrient media with different constituents were discovered for choosing one for long-time cultivation of calli. Induction of somatic embryogenesis was carried out on the nutrient media with high auxins content. Ethanol extracts from the intact plant and calli cultures were assayed with HPTLC PRO SYSTEM (CAMAG AG, Switzerland).Results and discussion. After two weeks of cultivation, the formation of primary callus was observed on the surface of the explants. The Linsmaier – Skoog medium with a reduced amount of sucrose (20 g/l) was recognized as the most suitable medium for long-term maintenance of cultures. Embryoid structures of Aralia cordata have been obtained, now we are continuing to collect analytics data about this process. Qualitative analysis of the extracts showed that callus cultures accumulate triterpene glycosides, their composition is close to that of the intact plant.Conclusion. A viable strain of callus culture of Aralia cordata Thunb. was obtained, a nutrient medium for long-term cultivation of calli was established. Somatic embryoids have been obtained, and their further development is currently being monitored. A preliminary phytochemical study showed that the composition of the chemical components of calli is close to that of an intact plant.

Comparative Analysis of Maceration and Soxhlation for the Extraction and Preliminary Phytochemical Screening of the Roots of Cassia fistula L.

Cassia fistula L., also known as ‘Golden shower tree’ or ‘Indian laburnum’ of the Fabaceae family, is a common ornamental tree with various medicinal uses. It is a medium or large-sized deciduous tree found in Asian countries, especially in India, Myanmar, Sri Lanka, Thailand, etc. This species has cultural importance as it is the national tree of Thailand and the flower is considered as the state flower of Kerala. Various medicinal activities are identified on basis of traditional knowledge. This research article enlightens the physicochemical characters, comparative successive solvent extraction, and qualitative phytochemical screening of various extracts of Cassia fistula L. roots. The present study aims to compare successive solvent extraction (maceration and soxhlation) for the estimation of yield and qualitative phytochemical screening of extracts obtained through both extractions. Two methods of successive solvent extraction were done, i.e., maceration and soxhlation using solvents of increasing polarity such as petroleum ether, chloroform, ethyl acetate, methanol, and distilled water. The phytochemical tests were carried out for each extract using standard methods of analysis and these investigations. The yields of extract were calculated as %w/w in all five solvents. Comparison of extraction methods helps to determine which method gives the maximum yield of extraction and preliminary phytochemical studies help to find out the secondary metabolites present in the extracts from both methods and compare the results.

Development of Pharmacognostic Profile and Preliminary Phytochemical Study of Pluchea wallichiana DC

Abstract: Introduction: The genus Pluchea comprises of around 80 species native to tropical and warm temperate areas. It has been traditionally known as rasna and used to treat different ailments including inflammation and arthritis. One of the species of Pluchea, Pluchea wallichiana DC (Family: Asteraceae), commonly known as wallich camphor weeds. Aim and Objectives: The present study aims to develop pharmacognostic profile, set the qualitative and quantitative characteristics of phytochemicals present in leaf, stem and root parts of plant and determine antioxidant potential. Materials and Methods: The plant profile development includes authentication, macro- microscopical, physico-chemical, and chromatographic evaluation of root, stem and leaf of the plant. The presence of essential minerals in the plant was confirmed using ICP-MS. The DPPH technique was used to assess the antioxidant potential. Results: P. wallichiana is an erect branched shrub with camphoraceous, green, alternate, broadly obovate and sessile leaves and white compound corymb flowers. The stems are circular and green, and the tap root is branched, cylindrical and brownish in color. Microscopy of leaf, stem and root has demonstrated clear anatomical features. The phytochemical screening unveils the presence of steroids, terpenoids, flavonoids, tannins, and coumarins. The HPTLC fingerprinting profile was developed from the mentioned plant parts. The leaf expressed higher percentage of phenolics, flavonoids and steroids as compared to root and stem. ICP-MS analysis demonstrated presence of calcium, potassium, magnesium, zinc, and iron present as a mineral in leaves. The concentration of leaf methanol extract required to inhibit 50% of DPPH free radicals was 29.49 μg/mL. Conclusion: The developed pharmacognostic and phytochemical parameters may help to establish standards for identification, quality, and purity of Pluchea wallichiana DC. Keywords: Pluchea wallichiana, Morphology, Microscopy, Flavonoids, HPTLC, Antioxidant activity, Mineral element.

Preliminary Phytochemical and Physicochemical Study of Cuscuta Chinensis L. Aqueous Extract Compared with Previous Studies

Preliminary Phytochemical and Physicochemical Study of Cuscuta Chinensis L. Aqueous Extract Compared with Previous Studies

Assessment of Coccinia Indica root extract for antiulcer activity in experimental animals

The objective is to evaluate the anti–ulcer activity of ethanolic extract of root of Coccinia grandis (Linn.), Anti-ulcer activity of the three extract was studied in rats by using pylorus ligated ulcer model and it was subjected to preliminary phytochemical studies for the identification of phytoconstituents and also studied for color, consistency and percentage yield of various extracts. Ranitidine was used as the standard drug for comparison. The animals were sacrificed after 06 hrs after the ligation. Stomach was dissected out and contents were drained into tubes and were centrifuged and volume was noted. The PH of gastric juice was recorded using a PH meter. The contents were subjected for analysis of free and total acidity and Na+, k+ ion concentration. The numbers of ulcers per stomach was noted and severity of ulcers scored. Then the blood samples were collected and subjected to estimation of serum calcium and alkaline phosphatase level. The expected result is to get an anti-ulcer activity of the leaf extracts of Coccinia grandis should owing to the presence of one or more phytoconstituents, which may reduce the acidity of the gastric juice and also prevents the mucosal damage and ulcer formation. The Ethanolic Extract 400mg/kg expected to showed comparable anti ulcer-activity as that of standard Ranitidine.

Phytochemical and pharmacological evaluation of anti-acne activity of herbal extract of Cassia angustifolia

P. acnes, as the foremost causative microorganism, M. furfur (yeast), S. Epidermidis are present in acne lesions. So in the present study ethanolic extract of Cassia angustifolia were selected for the anti-acne activity. Results of phytochemical screening of Cassia angustifolia flavonoid, proteins, alkaloids, saponins and diterpenes were detected in ethanol extracted. All phytochemical are absent in pet. ether extract of Cassia angustifolia . In thin layer chromatography from the Rf value (0.60) it was confirmed the presence of Quercetin as flavanoids compound in the extract. The preliminary phytochemical study was carried out according to standard literature. This revealed that they contain various phytoconstituents which can be responsible for the anti-acne activity. The acne-like inflammatory activity was carried out by measuring the ear thickness. Ethanolic leaves extract of Cassia angustifolia showed a significant reduction in the ear thickness. It seems that the increased ear thickness and inflammation caused due to various biochemicals, viz. various kinins, histamine and 5-HT is significantly reduced. The presence of various phytoconstituents including the hydroalcoholic leaves extract of Cassia angustifolia i.e. flavanoids, phenols showed significant anti-acne properties.