Standardization and Phytochemical screening of Abutilon crispum

Abstract

The main objective of the research is to recognize, validate and also to avoid the adulteration of plant Abutilon crispum. The correct identification of the plant Abutilon crispum was possible because of preliminary phytochemical screening, extraction by maceration and standardization. Preliminary phytochemical screening and TLC studies were carried out by standard methods with extracts such as chloroform, ethyl acetate and methanol. While standardization comprises the evaluation of parameters like pH range, water-soluble ash, loss on drying, total ash, acid insoluble ash, sulfated ash, ethanol-extractable parameters. The following values are found to be the results of the above testsi.e, loss on drying and total ash values are 7.2±0.22 and 6.32±0.04 correspondingly, water soluble ash was 1.33±0.05. Preliminary phytochemical evaluation for these extracts discovered the occurrence of flavonoids, steroids, terpenoids, glycosides and alkaloids in this plant. In methanol extract also major components such as alkaloids, flavonoids, tannins and glycosides were found. The thin layer chromatography examination of Abutilon crispum displayed the occurrence of steroids with0.76 and 0.52 Rf values in diverse solvent systems. TLC investigation of extract of ethyl acetate exhibited Rf values 0.74 and 0.81 due to occurrence of flavonoids with in dissimilar solvent systems similarly. The evidence found from contemporary study deliver valuable data that will be supportive in identifying and to perform upcoming research in future.