Assessment of Coccinia Indica root extract for antiulcer activity in experimental animals

Abstract

The objective is to evaluate the anti–ulcer activity of ethanolic extract of root of Coccinia grandis (Linn.), Anti-ulcer activity of the three extract was studied in rats by using pylorus ligated ulcer model and it was subjected to preliminary phytochemical studies for the identification of phytoconstituents and also studied for color, consistency and percentage yield of various extracts. Ranitidine was used as the standard drug for comparison. The animals were sacrificed after 06 hrs after the ligation. Stomach was dissected out and contents were drained into tubes and were centrifuged and volume was noted. The PH of gastric juice was recorded using a PH meter. The contents were subjected for analysis of free and total acidity and Na+, k+ ion concentration. The numbers of ulcers per stomach was noted and severity of ulcers scored. Then the blood samples were collected and subjected to estimation of serum calcium and alkaline phosphatase level. The expected result is to get an anti-ulcer activity of the leaf extracts of Coccinia grandis should owing to the presence of one or more phytoconstituents, which may reduce the acidity of the gastric juice and also prevents the mucosal damage and ulcer formation. The Ethanolic Extract 400mg/kg expected to showed comparable anti ulcer-activity as that of standard Ranitidine.