Landsat 9 Operational Land Imager (L9-OLI) was launched on 27 September 2021, after completing a successful radiometric pre-launch calibration and characterization phase. The radiometric math model that governs the ground system—the data processing and analysis system (DPAS)—uses various calibration parameters that had been derived based on the pre-launch tests and analysis. During the on-orbit commissioning phase, the OLI system acquired specific sets of data collects, which enabled the revalidation of the pre-launch absolute calibration scale and other associated instrument performance characteristics. The analysis results shown in this paper focus on the activities and results related to the transfer-to-orbit analysis for the SI-traceable pre-launch radiometric scale. Key topics discussed in this paper include: radiance and reflectance calibration parameters for OLI; solar diffuser collects; stimulation-lamp collects; dark response; signal-to-noise ratios; and noise characteristics; radiometric response stability and the on-orbit update to the radiance to reflectance conversion factors. It will be shown that the OLI response during the early on-orbit operation matched pre-launch results and therefore this re-validates the absolute radiometric scaling at the predicted pre-launch level within the expected level of uncertainties. The launch did not cause any significant changes to the OLI system from the perspective of the absolute radiometric calibration performance. Once the transfer to orbit of the absolute calibration was confirmed, it created a solid basis for further on-orbit refinements of the radiance calibration parameters. As such, follow-on calibration refinements are discussed in other articles within this special issue, and they address issues such as uniformity as well as cross-calibration activities.