During the archaeological survey research project ‘Island Cultures in a Diachronic Perspective: the case of Therasia’, large amounts of pottery were recorded throughout the island of Therasia, ranging in date from the Bronze Age to modern times. Focusing on the prehistoric period, pottery of the Early Cycladic and late Middle Cycladic periods was recovered at Panaghia Koimisis, which is situated on the southern part of the island. This paper presents the petrographic data and results of the analysis carried out on pottery samples which are representative of variable surface treatments and different macro-fabrics of these two prehistoric periods. Tackling issues of provenance and technology, the current scientific analysis attests the coexistence of Theran and off-Theran pottery fabrics already at Panaghia Koimisis in the Early Cycladic period. The majority of the pottery fabrics at Panaghia Koimisis were identified as Theran and the analysis demonstrates intensive contacts between the southern parts of Thera and Therasia throughout the Early and late Middle Cycladic phases. Moreover, adding support to previous studies, this research indicates a wide Cycladic pottery network, in which the site participated as a consumer. During the late Middle Cycladic period major changes in the Theran production are documented, including the disappearance of the earlier pottery recipe, which had been prevalent at Panaghia Koimisis.