Predominant Breed Research Articles

Factors associated with the evolution of digit health in Swiss dairy herds in a nationwide digit health program.

The purpose of this study was to investigate the evolution of digit health (DH) on Swiss dairy farms participating in a nationwide DH program and to identify risk factors associated with poor DH. Specially trained claw trimmers recorded disorders of the digits (DOD) electronically during routine trimmings between January 2020 and June 2023. The first part of the study was a non-randomized controlled implementation study, comparing the evolution of DH in 75 herds that received professional on-farm risk assessments as well as veterinary advice with 49 herds that did not. Overall DH improved over time in both groups, with no difference between the groups. Differences emerged when implementation rates of measures after on-farm risk assessment were considered: DH of farms implementing >50% of recommended measures improved significantly more compared with farms implementing £ 50%. Also, farms where cows predominantly suffered from infectious-related lesions improved significantly compared with farms where cows suffered predominantly from mechanical-metabolic-related lesions. The second part of the study consisted of a retrospective observational risk factor analysis with a larger population (498 farms). Here, greater improvement of DH on a farm over the study period was associated with a higher Farm-Claw-Score (FCS; index for decreased DH) at the beginning of the study and longer duration of participation in the DH program. Moreover, less improvement of DH was associated with freestalls, with "valley area" farm sites and with herds with Holstein Friesian cows as predominant breed. Our results suggest that, if implemented appropriately, measures issued by DH experts may improve DH. Furthermore, voluntary participation in a DH program in combination with routine electronic recording of DH data by claw trimmers seem to raise farmers' motivation to improve DH. Our results are useful for farmers and veterinarians as well as for establishing DH programs in other countries.

The growing, resilient, inclusive and sustainable (GRINS) project for the development of life cycle inventory databases of beef cattle raised in Italy: The statistical datasets and the environmental assessment

Within the framework of the Growing, Resilient, Inclusive and Sustainable (GRINS) project (Spoke 1, WP3, Next Generation EU program), this work aims to overcome the absence of Italian beef cattle Life Cycle Inventory (LCI) datasets through a capillary analysis of several parameters. Specifically, the contribution to the environmental impact of livestock breeding of breed features (age, gender, weight, daily weight gain, breeding, feed intake and composition, milk and manure production), as well as stable management and crop cultivation was investigated. Statistical inventory datasets (84 in total) were developed for the predominant (<1 % population cut-off) beef cattle breeds in Italy.A key finding was the quantification of CH4 emissions from enteric fermentation (ranging from0.259 to 0.714 g kg−1 of live weight per day) and its contribution to the overall environmental impact of beef cattle breeding. The composition of feed rations emerged as critical, influencing both cattle emissions and environmental impacts associated with the cultivation and transport of raw materials. Intensive and langer breeds like Aubrac, Blond d'Aquitaine, Blue Belga, Charolaise, and Chianina, exhibited higher eco-indicator values compared to the extensive beef cattle breeds (Podolica, Highland, and Maremmana). The life cycle assessment identified several key impact categories (climate change, water use and ecotoxicity freshwater) mainly contributing to the total eco-indicator. Climate change (22.1 %) represented the greatest impact category, with beef cattle emissions over their lifespan averaging 9.3 Mg CO2-eq. Methane (enteric fermentation) and NH3 (manure management) emissions, as well as irrigation and pesticide use, represented the main hotspots. A comparative analysis evaluated the environmental footprint of Italian beef cattle against benchmarks outlined in the “Made Green in Italy” brand's Product Category Rules. This comparison revealed a 32.4 % reduction in total eco-indicator for Italian beef cattle, due to a significant decrease in freshwater ecotoxicity (−72.5 %), land use (−34.2) and climate change (−7.5 %).

Delineating marker genotypes for higher reproduction performance to aid in selection of Murrah bulls

Unraveling the genetic variability of performance traits is the key basis of selection in farm animals. Murrah is a predominant milch breed of India, efforts were being made in the past to select them for higher production, which is negatively related with the fertility. Keeping this fact in mind and to select the animals having optimum production and fertility performance, present study was conducted to validate suitable markers and the association of their allelic variation with the fertility traits in Murrah buffaloes. Study was conducted on 280 Murrah buffaloes to explore SNPs in SPEF2 gene and their association with sire conception rate (SCR), conception rate (CR) and weight at birth of Murrah bulls. Results revealed that SNP C168T of SPEF2 gene with genotype CC was found to have non-significant but higher CR and SCR in comparison to CT and TT genotype. Although no significant association of the identified SNPs could be established with bull fertility traits, a further study with a large number of samples could make the differences due to genotypes a significant effect and can be used as an aid for early selection of high fertile Murrah bulls

Benchmarking claw health across three dairy cow breeds in 508 dairy farms using numerical claw health indicators

Currently, prevalence and incidence of claw lesions are used as parameters for benchmarking claw health. The aims of this study were to create a benchmarking system for claw health utilizing the claw health indicators Farm-Claw-Score (FCS) for the herd and Cow-Claw-Score (CCS) for the individual animal, and to benchmark claw health of the three predominant dairy cattle breeds in Austria. Claw health data from 17,642 cows from 508 Austrian dairy farms were analyzed. The CCS and FCS were calculated based on recorded claw lesions and their three severity levels using geometrically weighted scoring. The FCS of each of the dairy farms was classified into five percentile thresholds (P10, P25, P50, P75, P90), with the FCS calculated using the median value of CCS in each herd. Furthermore, claw health was benchmarked for three breeds (Fleckvieh, Holstein, Brown Swiss cows), using claw lesion prevalences and CCS values.When the median FCS was calculated, dairy farms in P50 and below had an FCS of 20.0, indicating very good claw health. However, P90 farms showed an FCS-MEDIAN of 67.5. Evaluation of the prevalences of the 14 claw lesions considered and the CCS values revealed that Fleckvieh cows (CCS-MEDIAN: 24.0), followed closely by Holstein cows (CCS-MEDIAN: 22.7) had significantly poorer claw health (P < 0.0001) compared to Brown Swiss cows (CCS-MEDIAN: 12.0). The use of CCS and FCS as primary claw health indicators allowed for a quick assessment of the current state of an individual cow and a dairy herd in a benchmarking system. Detailed information on the claw health of each animal and the dairy herd can be easily reviewed by examining diagnosis lists that display prevalences, particularly those related to lameness, in the respective electronic documentation systems.

Genomic selection strategies for the German Merino sheep breeding programme - A simulation study.

Genomic selection is widely implemented in livestock breeding programmes across species. Its potential is also evident for sheep breeding; however, it has several limitations, particularly because of the high genetic diversity across and within sheep breeds. In Germany, the predominant sheep breed is the Merino sheep. Until now, there has been no use of genomic selection in the German Merino sheep breeding programme. In this simulation study, different genomic selection strategies were compared with a reference scenario with a breeding value estimation based on pedigree BLUP. A simplified version of the German Merino sheep breeding programme, including a health and a production trait in the breeding goal, was simulated via the R package Modular Breeding Program Simulator (MoBPS). Real genotype data were used to create a population specific simulation. The reference scenario was compared with several alternative scenarios in which selection was based on single-step GBLUP (ssGBLUP) breeding value estimation with varying genotyping strategies. In addition to scenarios in which all male and all male plus all female lambs were genotyped, scenarios with a preselection of lambs, that is only a certain proportion (top 25%, top 50%) genotyped, were simulated. The results revealed that genetic gain increased with increasing numbers of available genotypes. However, marginal gains decreased with increasing numbers of genotypes. Compared with the reference scenario, genotyping the top 25% of male lambs increased the genetic gain for the breeding ram population by 13% for both traits whereas genotyping the top 50% of male lambs or all male lambs led to increases of 18% (17%) or 26% (21%) for the health (production) trait, respectively. The potential of genotyping females in addition to male lambs was less evident on the male side with no significant differences between the scenarios with different proportions of genotyped females. The results have shown that genomic selection can be a valuable tool to increase genetic gain in the German Merino sheep population and that the genotyping of a certain proportion of animals might lead to substantial improvement over pedigree-based breeding value estimation. Nevertheless, further studies, especially economic evaluations, are needed before practical implementation.

Population dynamics of cattle slaughtered in a transient slab of the international cattle market Ibadan, Nigeria

The influx of cattle into Nigerian abattoirs is on the increase with little or no documentation on slaughtering activities and types of cattle slaughtered. This study was therefore conducted to evaluate the Population dynamics of cattle slaughtered in the study area. The study was carried out at Akinyele international cattle market, Ibadan over a period of 5 weeks between 7am-7pm to ascertain information on the population of cattle slaughtered, breeds, sex, size and the time of slaughtering. The result showed that a total of 1839 cattle were being slaughtered. These include White Fulani (43.2%), Red Bororo (35%), Gudali (19.8%) and Kuri (2%). Slaughtering activities starts at 7am with most of the animals (62.7%) slaughtered between 7-10am from Monday to Sunday. The highest number of cattle is processed on Fridays (35.9%) while the lowest are on Sundays (4.5%). More female cattle (73.3%) were slaughtered compared to 16.7% for male cattle with 57.2% (medium) of the cattle in the mid-age category. White Fulani and Red Bororo were the predominant breeds of cattle slaughtered at Akinyele international cattle market, Ibadan.

Evaluation and use of recently introduced sheep breeds to Uruguay

Sheep numbers in Uruguay have declined over the last 30 years. In the north and northeast there are extensive wool and dual-purpose systems, respectively, where both wool and meat impact on income. In the south, small-scale family sheep meat production systems have flourished. In this thesis I report work pertaning to production systems in the south (1) and northeast (2) regions. Corriedale (C) is the predominant breed in the country. Milchschaf (M) has been disseminated in the south but C still prevails in the northeast. There is a paucity of rigorous evaluations of these breeds or alternatives that could be more profitable, such as Highlander (H) for the south and Dohne Merino (DM) for the northeast. I experimentally assessed: in 1), wool production, reproduction and growth in C, H and M, and in 2), wool quality and body traits in ewe hoggets from rotational crossbreeding between C and DM. In 1), wool production was better in C, but H and M outperformed C in live weight and reproductive performance. In 2), genotypes with greater proportions of DM produced lower fleece weight, and those with greater proportions of C produced coarser fibre diameter, with no differences in wool quality or morphological traits related to meat aptitude. Gross margins (GM) integrate physical performance with product values and costs, allowing monetary comparison between genotypes. I calculated GMs for a number of production and market scenarios. In 1), most often GM was greatest for H, followed by M. The differences in favor of H and M over C decreased when I considered higher feed requirements for H and M. The GM of H and M increased by assuming they were wool-less (the cost of harvesting exceeds the value of the wool produced). In 2), I considered low and high prices for wool of different fibre diameter. GM was greatest for ¾DM and ⅝DM at low prices, and for ¾DM and ½DM at high prices. C had the lowest GM in all scenarios. I recommend H for southern systems and research on hair sheep breeds. In extensive dual-purpose systems, rotational crossbreeding between C and DM allows for improved wool income without losing meat potential. I formulate recommendations about future genotype evaluations, integrating them with current within breed genetic evaluations.

A comparison of genetic and genomic breeding values in Saanen and Alpine goats

Nowadays, several countries are developing or adopting genomic selection in the dairy goat sector. The most used method to estimate breeding values is Single-Step Genomic Best Linear Unbiased Prediction (ssGBLUP) which offers several advantages in terms of computational process and accuracy of the estimated breeding values (EBVs). Saanen and Alpine are the predominant dairy goat breeds in Italy, and both have similar breeding programs where EBVs for productive traits are currently calculated using BLUP. This work describes the implementation of genomic selection for these two breeds in Italy, aligning with the selection practices already carried out in the international landscape. The available dataset included 3 611 genotyped animals, 11 470 lactation records, five traits (milk, protein and fat yields, and fat and protein percentages), and three-generation pedigrees. EBVs were estimated using BLUP, GBLUP, and ssGBLUP both with single and multiple trait approaches. The methods were compared in terms of correlation between EBVs and genetic trends. Results were also validated with the linear regression method excluding part of the phenotypic data. In both breeds, EBVs and GEBVs were strongly correlated and the trend of each trait was similar comparing the three methods. The average increase in accuracy across traits and methods amounted to +13 and +10% from BLUP to ssGBLUP for Alpine and Saanen breeds, respectively. Results indicated higher prediction accuracy and correlation for GBLUP and ssGBLUP compared to BLUP, implying that the use of genotypes increases the accuracy of EBVs, particularly in the absence of phenotypic data. Therefore, ssGBLUP is likely to be the most effective method to enhance genetic gain in Italian Saanen and Alpine goats.

Sero-Prevalence of Leptospira pomona Infection among Cattle Slaughtered in Sokoto Metropolitan Abattoir, Sokoto State, Nigeria

Aim: Leptospirosis is a transmissible zoonotic disease that is widely distributed worldwide, it causes public health problems and affects both developed and developing countries. This study investigated sero-prevalence of Leptospira pomona infection among cattle slaughtered in Sokoto State Metropolitan Abattoir. Methods: One hundred and eighty-four (184) samples were collected aseptically from the jugular vein of different breeds of cattle (Red Bororo, Sokoto Gudali and White Fulani) at slaughter position in Sokoto metropolitan abattoir using aseptically anticoagulant-free vacutainers twice in a week and the samples were centrifuged and sera separated. Indirect Enzyme Linked-Immunosorbent Assay (ELISA) technique (specific for the detection of antibodies against Leptospira pomona and L. hardjo in bovine serum) was employed and the procedures were carried out according to the manufacturer’s protocol (ELISA Bovicheck® Lepto HP, Bioveta, Canada). The optical density was read using an ELISA reader (Optic System IVYMEN® 2100C, USA). Results: Overall prevalence of Leptospira pomona in the study area was found to be 25 % (46). The prevalence in cows was higher (25.3%) than that recorded in males (24.8%). There was absence of a statistically significant difference (p&gt;0.05) in seropositivity of leptospiral infection between the bulls. The age-specific prevalence of L. pomona infection showed that adult cattle are more exposed, but no statistical association was observed p&gt;0.05. The breed specific prevalence of Leptospira pomona infection showed that Sokoto Gudali recorded a higher prevalence of 28% (7), while Red Bororo and White Fulani recorded a prevalence of 24.8% (26) and 20.4% (11) respectively. There was no statistical association between age and Leptospiral infection (p&gt;0.05). The most predominant breed in the study area was the White Fulani cow. Conclusions: This study concluded that cattle in the study area are exposed to infection by Leptospira spp. The presence of leptospirosis among cattle slaughtered in the abattoir may serve as an occupational hazard to the abattoir workers. These abattoir workers are at risk of being infected by Leptospira spp. serovar pomona. Age, sex, and breed are not associated with Leptospira infection in cattle slaughtered in Sokoto State abattoir.

Farm management and husbandry practices associated with spontaneous humeral fractures in New Zealand dairy heifers

ABSTRACT Aims To use a farm-based survey to identify characteristics of the New Zealand dairy system associated with the risk of spontaneous humeral fracture in dairy heifers. Methods A questionnaire was designed and made available in print and online to collect information from dairy farmers and/or veterinarians, across New Zealand, about the management and nutrition of cows from birth to first lactation. Data were collected from July 2019 to March 2020 from farms that either had recorded (case farms) or not recorded (control farms) cases of humeral fractures in dairy heifers. Results A total of 68 completed questionnaires were returned, with 35 responses from case farms and 33 responses from control farms. Twenty-six responses (38%) were from the South Island (13 case farms and 13 control farms) and 38 responses (56%) were from the North Island (20 case farms and 18 control farms). For four questionnaires (6%) farm location was not given. Adjusting for the effect of age when calves accessed pasture, case farms had increased odds of having Holstein-Friesian Jersey crossbreed cows as the predominant breed (OR = 9.7; 95% CI = 3.1–36.0; p < 0.001). Adjusting for the effect of breed, allowing calves access to pasture a week later decreased the odds of being a case farm (OR = 0.68; 95% CI = 0.47–0.90; p = 0.006). Conclusions Cows being Holstein-Friesian Jersey crossbreed was identified as a possible risk factor associated with spontaneous humeral fracture in dairy heifers in New Zealand. Given the small sample size, the likely multifactorial aetiology for humeral fractures, and the non-randomised survey, this risk factor, and the possible association between age at turn out and herd production with humeral fractures, all require further investigation.

Description of cattle production systems in different agro-ecological zones of Narok County, Kenya

Cattle farming is an integral part of the livelihoods of farmers in Narok County. However, very few studies have been conducted to describe livestock production in different agroecosystems in Kenya. The objective of this study was to determine cattle production systems and characteristics under different agro-ecosystems. The study was conducted through the administration of semi-structured questionnaires to 817 farmers in three sublocations from Narok south sub-county. The main livestock production systems were pure pastoral, communal grazing and zero-grazing where indigenous cattle were the predominant breed kept (86.8%) with a few farmers keeping crossbreeds or exotic breeds. However, sheep (75.1%) goats (79.4%), chicken (68.6%) and donkeys (32.9%) were also kept. The mean lactation period for cattle was 9.5 months with average inter-calving interval of 14.1 months. More households (45.1%) grazed cattle within their own pastureland compared to pure pastoralism (12.5%), communal grazing (8.0%), zero grazing (0.9%), or combination (33.5%). Approximately 37.1% of the respondents herded their cattle, 13.1% were on free grazing, 36.6% both free grazing and herding whereas 13.2% used paddocks. The mean land area under pasture was estimated at 53.9 acres. The main source of drinking water was within the grazing field (63.6%) with 36.4% having had to take livestock elsewhere. Most households (85.7%) practicing mixed farming could easily access veterinary services compared to 57.2% and 55.1% in agro-pastoral and pure pastoral system respectively (p&lt;0.0001). The main veterinary interventions were deworming, vaccination, and antibiotic administration. Most households (98.6%) had sprayed their livestock with 98.9% sourcing the products from agrovet outlets. More respondents (63.8%) in agro-pastoral compared to 61.5% and 49.7% from pastoral and mixed farming respectively were aware of East Coast Fever disease (p&lt;0.0001). Cattle were principally fed on natural pasture either produced from owned or leased lands with farmers getting unequal access to veterinary services.

Retrospective study of acupuncture treatment for canine thoracolumbar intervertebral disc herniation

Thoracolumbar interverbal disk herniation (TL-IVDH) is a common neurological disorder in dogs, which often affects the life quality of patients and their caregivers. Acupuncture is increasingly accepted by veterinarians and animal owners, and has been used in the treatment of neurologic disorders. This study aims to evaluate the effects of acupuncture treatment for canine TL-IVDH. Clinical cases from 2016 to 2021 were collected. According to the inclusion and exclusion criteria, 94 TL-IVDH cases that received acupuncture treatment were selected. Clinical classification and efficacy criteria were formulated based on the Frankel Score. For each case, clinical presentation, improvement, recovery and recurrence were recorded. Male dogs with TL-IVDH accounted for 64% of all cases. The average age was 7.49 years old, the predominant breed was poodle, and the incidence site was concentrated from T11 to L1. The overall recovery rate after receiving acupuncture treatment was 79.78%, which was comparable when considering only cases with grade 2, 3, or 4 spinal cord lesions (83.33% ~ 85.19%). In conclusion, canine with grade 2, 3, and 4 spinal cord lesions due to TL-IVDH receiving acupuncture had a high recovery rate, suggesting acupuncture could be a viable treatment options for canine with TL-IVDH.

Effect of Metabolizable Protein Supply on Milk Performance, Ruminal Fermentation, Apparent Total-Tract Digestibility, Energy and Nitrogen Utilization, and Enteric Methane Production of Ayrshire and Holstein Cows

In North America, the nutrient requirements of dairy cattle are predicted using the Cornell Net Carbohydrate and Protein System (CNCPS) or the National Research Council (NRC). As Holstein is the most predominant dairy cattle breed, these models were developed based on the phenotypic, physiological, and genetic characteristics of this breed. However, these models may not be appropriate to predict the nutrient requirements of other breeds, such as Ayrshire, that are phenotypically and genetically different from Holstein. The objective of this study was to evaluate the effects of increasing the metabolizable protein (MP) supply using CNCPS on milk performance, ruminal fermentation, apparent total-tract digestibility, energy and N utilization, and enteric methane production in Ayrshire vs. Holstein lactating dairy cows. Eighteen (nine Ayrshire; nine Holstein) lactating cows were used in a replicated 3 × 3 Latin square design (35-d periods) and fed diets formulated to meet 85%, 100%, or 115% of MP daily requirement. Except for milk production, no breed × MP supply interaction was observed for the response variables. Dry matter intake (DMI) and the yields of energy-corrected milk (ECM), fat, and protein were less (p < 0.01) in Ayrshire vs. Holstein cows. However, feed efficiency and N use efficiency for milk production did not differ between the two breeds, averaging 1.75 kg ECM/kg DMI and 33.7 g milk N/100 g N intake, respectively. Methane yield and intensity and urinary N also did not differ between the two breeds, averaging 18.8 g CH4 /kg DMI, 10.8 g CH4 /kg ECM, and 27.6 g N/100 g N intake, respectively. Yields of ECM and milk protein increased (p ≤ 0.01) with increasing MP supply from 85% to 100% but no or small increases occurred when MP supply increased from 100 to 115%. Feed efficiency increased linearly with an increasing MP supply. Nitrogen use efficiency (g N milk/100g N intake) decreased linearly (by up to 5.4 percentage units, (p < 0.01) whereas urinary N excretion (g/d or g/100 g N intake) increased linearly (p < 0.01) with an increasing MP supply. Methane yield and emission intensity were not affected by MP supply. This study shows that feed efficiency, N use efficiency, CH4 (yield and intensity), and urinary N losses did not differ between Ayrshire and Holstein cows. Energy-corrected milk yield and feed efficiency increased, but N use efficiency decreased and urinary N losses increased with increasing dietary MP supply regardless of breed. Ayrshire and Holstein breeds responded similarly to increasing MP levels in the diet.

Genetic association of wool quality characteristics in United States Rambouillet sheep.

Introduction: Fine wool production is an important source of revenue, accounting for up to 13% of total revenue in extensively managed wool sheep production systems of the United States. The Rambouillet are a predominant breed that excels in wool quality characteristics. Understanding the genetic basis of wool quality characteristics would aid in the development of genomic breeding strategies to facilitate genetic improvement. Methods: Wool characteristics and DNA were collected for rams enrolled in the North Dakota State University and University of Wyoming annual central performance ram tests over a three-year period (2019-2021, N = 313). The relationships of wool quality characteristics including grease fleece weight adjusted 365days (wt. 365 adj.), clean fleece wt. 365 adj., staple length 365 adj., average fiber diameter, face wool cover, amount of skin wrinkles and belly wool were evaluated through genome-wide association studies (GWAS), Pearson correlation and ANOVA. Results: The GWAS identified four genome-wide significant genetic markers (p-value <1.19e-06) and five chromosome-wide significant markers (p-value <1.13e-05) on chromosomes 1, 2, 4, 15, and 19. Significant markers were associated with genes notable for relevant wool biological functions, including the gene ABCC8 which codes for SUR1, an ATP-sensitive potassium channel known to affect hair growth and 60S ribosomal protein L17-like, previously found to be expressed during follicle formation. The strongest Pearson correlation coefficients were identified between clean fleece wt. 365 adj. and grease fleece wt. 365 adj. (r = 0.83) and between clean fleece wt. 365 adj. and staple length 365 adj. (r = 0.53). Additionally, clean fleece wt. 365 adj. was correlated with final body weight (r = 0.35) and scrotal circumference (r = 0.16). Staple length 365 adj. (p-value = 5e-04), average fiber diameter (p-value = .0053) and clean fleece wt. 365 adj. (p-value = .014) were significantly associated with belly wool score. Discussion: The results of this study provide important insight into the relationships between wool quality characteristics and report specific markers that Rambouillet sheep producers may use to help inform selection and breeding decisions for improved wool quality.

Sero-epidemiology of brucellosis in small ruminants on rangeland in northern Mexico

The seroprevalence of Brucella antibodies in serum of goats and sheep reared on semi-arid pastoral systems of northeastern Mexico was assessed. Additionally, species, gender, and predominant breed were evaluated as risk factors for seropositivity to brucellosis. Data were from 375 flocks of goats or co-mingled goats and sheep. Serum samples from 11001 goats and 4741 sheep collected between 2016 and 2019 were analyzed with the brucellosis card test. The disease affected 12% of flocks. The overall prevalence of brucellosis seropositivity in goats and sheep were estimated at 2.1% (95% CI = 1.78-2.31) and 0.8% (95% CI = 0.58-1.10), respectively. Brucellosis seropositivity was two times more likely (p &lt; 0.01) in goats than sheep. The risk of seropositivity to brucellosis for goats and sheep was 2.3 and 3.2 times higher (p &lt; 0.01) in females than males. Toggenburg goats were more likely (p &lt; 0.01) to be seropositive to brucellosis than all other breeds of goats, whereas criollo sheep were more likely to be seropositive to brucellosis compared to hair sheep composite breeds. The current study revealed that, despite the absence of Brucella vaccination, brucellosis is not widely distributed in the study area. Also of all animals screened, seropositivity to Brucella infection was highest in Toggenburg when compared to other dairy and meat breeds; Criollo sheep also presented the highest seropositivity to brucellosis compared to hair sheep composite breeds. Finally, females compared to males had increased odds of testing positive for brucellosis.

RACES COMPOSITION OF THE COLONIES OF HONEY BEE (APIS MELLIFERA L.) IN THE APIARIES OF THE NORTHERN BUKOVINA

Modern honey bee races have evolved from local natural subspecies. Due to this, native bees are adapted better to local climatic conditions and are less susceptible to the combined effects of negative factors that lead to colony extinction. However, the distribution of honey bee subspecies in Europe over the past century has been heavily influenced by humans. According to the plan of breed zoning of honey bees in Ukraine, Chernivtsi region is defined as the region of the Carpathian bee. However, interbreed hybrids were formed due to the uncontrolled introduction of other breeds to the area and spontaneous hybridisation between them and native bees. Due to the existence of such hybrid forms, the diversity of local bee ecotypes is lost and genetic polymorphism within the population increases dramatically. This increase in polymorphism has led to an increase in biodiversity, the emergence of numerous intermediate variants of morphometric characteristics, and the loss of valuable Carpathian bee traits. Modern phylogenetic studies of bees include the analysis of a set of morphometric external parameters: cubital index, discoidal displacement and proboscis length. An integrated approach to the study of these traits can reflect the evolution of honey bees and, accordingly, their belonging to specific subspecies or breeds. In the region as a whole, the predominant breeds were Carpathian, Ukrainian Steppe, and Grey Mountain Caucasian, which belong to the subspecies A. m. carnica (Carpathian ecotype), A. m. macedonica and A. m. caucasica, respectively. In addition, the hybrid forms contain features of the alpine variant of carnica and dark European bees (A. m. mellifera). It is shown that hybridisation within Northern Bukovina was chaotic, and no localities with preservation of Carpathian lines were found.

Survey of the use of beef semen in dairy herds in Pennsylvania and nearby states.

Because dairies across the United States have rapidly adopted breeding to beef breed sires, the use of beef semen has increased dramatically in recent years. The objective of this survey was to gather information about the use of beef semen by dairy producers in the Northeast United States to generate beef × dairy cattle for beef markets. The survey was conducted using the services of the Center for Survey Research at the Pennsylvania State University-Harrisburg campus. Respondents had two options for returning their responses: 1) mail the paper survey to CSR in the postage-paid business-reply envelope included in the mailing, or 2) complete the survey online via an open-access web survey link. A total of 669 surveys were received and a final number of 617 surveys were included in the responses based on completeness and validity of the responses. Because of the broad electronic distribution, a true response rate cannot be calculated. Of these, 463 (75.0%) were completed via returned paper survey, and 154 (25.0%) were completed via web, between November 9, 2021 and February 16, 2022. Of the 617 respondents, 539 were from Pennsylvania. Due to the large variations in returned survey copies by state, results are reported without state separation. Across all respondents, 69.7% reported milking 100 or fewer cows and over 90% of collected responses reported Holsteins as the predominant dairy breed in the Northeast. Only 18.8% of the respondents did not currently, nor plan to, breed with beef semen. Deciding which beef bulls to use on Northeast dairy farms was primarily based on the recommendation of the semen sales representative (54.5%) and the price of the semen purchased (42.3%). In addition, 89.7% of respondents cited using Angus genetics in their beef bull selections. However, there was no difference in reported profitability of crossbreeding between respondents who indicated using other beef breeds vs. those who indicated just using Angus (P ≥ 0.19). In conclusion, using beef sires on dairy females, regardless of the breed of beef sire, adds value to the resulting progeny from dairy farms in the Northeast.

Innovative approaches to the development of dairy cattle breeding in the Kaluga region

The use of innovative approaches to the development of breeding cattle in the region has significantly increased and improved the productivity of animals. So, over the past 5 years, gross milk production has increased by 192 thousand tons or 171%, milk yield per cow by 2705 kg and amounted to 8745 kg in 2021. The breed composition of cows has changed, the Holstein breed with a specific gravity of about 80% has become the predominant breed of dairy cattle in the Kaluga region. The selection of bulls is aimed only at the bulls-leaders of the Holstein breed and their sons. IT technologies are actively used in the management of regional dairy cattle breeding in the region.

Goat production systems of the central highlands of Veracruz, Mexico

Objective: This study was to characterize the goat production units of the central mountain area of the state of Veracruz, Mexico. Disign/methodology/approach: A multiple case study was carried out taking into consideration socioeconomic aspects, type of forage, goat feeding, reproduction, improvement, and health. Results: The results showed that goat and cheese production account for 50-100% of the producers' income. The forages grown and the type of goat feed used in the production units of the mountain area are: King grass, CT-115, maralfalfa, giant star grass, alfalfa, and corn. Animal reproduction is carried out by controlled mating and births take place at the beginning and end of each year. Flocks do not exceed 150 heads; the predominant breeds are Saanen and Alpine. The average daily production of milk ranges from 16 to 90 L, with a production of 0.75-3 L milk animal-1 day-1. Study limitations/implications: None Findings/conclusions: The activity is family-based and involves cultivation, animal management, and milk and artisanal cheese production. Further studies are required to confirm individual milk production.

Prophylactic effect of topical betaxolol and dorzolamide on the fellow eye in unilateral canine primary angle closure glaucoma: 60 cases (2016.1-2021.5)

This study was aimed to evaluate the prophylactic anti-glaucoma effect of topical 5% betaxolol (BTX) and 2% dorzolamide (DRZ) on the second eye in dogs with unilateral primary angle closure glaucoma (PACG). Medical records of 60 dogs with unilateral PACG who received prophylactic anti-glaucoma eyedrops in the second eye, from 2016 to 2021, were reviewed. The duration of prophylactic effects of BTX only and DRZ after BTX failure was calculated. The prophylactic effects of BTX were maintained on 28/60 (46.7%) eyes until last visit and BTX failure was observed on median 510 (range, 53-1,927) days in 32/60 (53.3%) eyes. After DRZ instillation in BTX failure eyes, the prophylactic effects were extended at median 610 (range, 157-2,270) days in 21/32 (65.6%) eyes. DRZ failure eyes (17/21, 81.0%) eyes required chemical ablation or surgical intervention due to uncontrolled intraocular pressure. The duration of prophylactic effects was decreased with aging (R² = 0.334, p = 0.006). The predominant breeds were Shih-Tzu (41.9%) and American Cocker Spaniel (30.6%) with no significant differences in survival curves (p = 0.210). The average prophylactic effects of BTX persisted more than 1.5 year and could be selected the first prophylactic eye drop in unilateral PACG. Also, early surgical intervention should be considered in prophylactic medications failure cases.