Prediction Of Radiation Effects Research Articles

Assessment of the Impact of Radioactive Emissions on Biota

Conceptual provisions on the assessment and prediction of radiation effects on biota are discussed. Interconnected components of radioecological assessments are identified with account for the redistribution of radionuclides in the environmental components and the associated levels of exposure in living organisms. An approach to solve problematic issues in developing migration and dosimetric models of assessing the impact of radioactive emissions on biota is proposed.

Dynamics of acutely irradiated skin epidermal epithelium in swine: modeling studies.

A mathematical model, which describes the dynamics of acutely irradiated skin epidermal epithelium in swine, is developed. This model embodies the key mechanisms of regulation of skin epidermal epithelium and the principal stages of development of its cells (basal, prickle, and corneal). The model is implemented as a system of nonlinear ordinary differential equations, whose variables and parameters have clear biological meaning. The modeling results for the dose- and time-dependent changes in basal and prickle cell populations are in a good agreement with relevant experimental data. The correlation between the experimental data on the dynamics of moist reaction in acutely irradiated swine skin epidermal epithelium and the corresponding modeling results on the dynamics of corneal cells is revealed. Proceeding from this, the threshold level of corneal cells, which indicates the appearance of the moist reaction, is found. All this bears witness to the validity of employment of the developed model, after appropriate identification, in the investigation and prediction of radiation effects on skin epidermal epithelium in humans.

Modeling analysis of the dynamics of thrombocytopoietic, granulocytopoietic and erythropoietic systems in irradiated humans

Biologically motivated mathematical models, which describe the dynamics of the thrombocytopoietic, granulocytopoietic and erythropoietic systems in irradiated humans, are developed and thoroughly investigated [ 1– 3]. These models are implemented as the systems of non-linear ordinary differential equations, whose variables and constant parameters have clear biological meaning. The modeling studies revealed general regularities and characteristic features of the dynamics of the aforementioned hematopoietic lineages in acutely and chronically irradiated humans. It is shown that the modeling predictions qualitatively and quantitatively agree with the respective clinical data for humans exposed to acute and chronic irradiation in wide ranges of doses and dose rates. The ‘lethal’ dose rate of chronic irradiation, which is evaluated in the framework of the granulocytopoiesis model, coincides with the real minimal dose rate of lethal chronic irradiation for humans. As for the thrombocytopoiesis and erythropoiesis models, the respective ‘lethal’ dose rates of chronic irradiation are very close to the real one for humans. All this bears witness to the validity of employment of the developed models in the investigation and prediction of radiation effects on human hematopoiesis. These models could provide a better understanding of the risks to health from the space radiation environment for astronauts on long-term space missions. The models could also be helpful in estimating the hazards for health of a population, which resides in contaminated areas after an accident.

Alteration of the Serum N-glycome of Mice Locally Exposed to High Doses of Ionizing Radiation

Exposure of the skin to ionizing radiation leads to characteristic reactions that will often turn into a pathophysiological process called the cutaneous radiation syndrome. The study of this disorder is crucial to finding diagnostic and prognostic bioindicators of local radiation exposure or radiation effects. It is known that irradiation alters the serum proteome content and potentially post-translationally modifies serum proteins. In this study, we investigated whether localized irradiation of the skin alters the serum glycome. Two-dimensional differential in-gel electrophoresis of serum proteins from a man and from mice exposed to ionizing radiation showed that potential post-translational modification changes occurred following irradiation. Using a large-scale quantitative mass-spectrometry-based glycomic approach, we performed a global analysis of glycan structures of serum proteins from non-irradiated and locally irradiated mice exposed to high doses of γ-rays (20, 40, and 80 Gy). Non-supervised descriptive statistical analyses (principal component analysis) using quantitative glycan structure data allowed us to discriminate between uninjured/slightly injured animals and animals that developed severe lesions. Decisional statistics showed that several glycan families were down-regulated whereas others increased, and that particular structures were statistically significantly changed in the serum of locally irradiated mice. The observed increases in multiantennary N-glycans and in outer branch fucosylation and sialylation were associated with the up-regulation of genes involved in glycosylation in the liver, which is the main producer of serum proteins, and with an increase in the key proinflammatory serum cytokines IL-1β, IL-6, and TNFα, which can regulate the expression of glycosylation genes. Our results suggest for the first time a role of serum protein glycosylation in response to irradiation. These protein-associated glycan structure changes might signal radiation exposure or effects.

Comparative Analysis of the Dynamics of Thrombocytopoietic, Granulocytopoietic, and Erythropoietic Systems in Irradiated Humans

Biologically motivated mathematical models, which describe the dynamics of the thrombocytopoietic, granulocytopoietic, and erythropoietic systems in irradiated humans, are thoroughly investigated. These models are the systems of nonlinear ordinary differential equations, whose variables and constant parameters have clear biological meaning. The modeling studies reveal general regularities and peculiarities of the dynamics of the aforementioned hematopoietic lines in acutely and chronically irradiated humans. It is shown that the predictions of the models qualitatively and quantitatively agree with the respective clinical data for humans exposed to acute and chronic irradiation in wide ranges of doses and dose rates. Moreover, the "lethal" dose rate of chronic irradiation, which is evaluated in the framework of the granulocytopoiesis model, coincides with the real minimal dose rate of lethal chronic irradiation for humans. As for the thrombocytopoiesis and erythropoiesis models, the respective "lethal" dose rates of chronic irradiation are very close to the real one for humans. All this bears witness to the validity of employment of the developed models in the investigation and prediction of radiation effects on human hematopoiesis.

Heavy-Section Steel Technology and Irradiation Programs—Retrospective and Prospective Views

In 1965, the Atomic Energy Commission, at the advice of the Advisory Committee on Reactor Safeguards, initiated the process that resulted in the establishment of the Heavy Section Steel Technology (HSST) program at Oak Ridge National Laboratory. In 1989, the Heavy-Section Steel Irradiation (HSSI) program, formerly the HSST task on irradiation effects, was formed as a separate program, and in 2007, the HSST/HSSI programs, sponsored by the U.S. Nuclear Regulatory Commission (USNRC), celebrated 40 years of continuous research oriented toward the safety of light-water nuclear reactor pressure vessels (RPVs). This paper presents a summary of results from those programs with a view to future activities. The HSST program was established in 1967 and initially included extensive investigations of heavy-section low-alloy steel plates, forgings, and welds, including metallurgical studies, mechanical properties, fracture toughness (quasi-static and dynamic), fatigue crack-growth, and crack-arrest toughness. Also included were irradiation effects studies, thermal shock analyses, testing of thick-section tensile and fracture specimens, and nondestructive testing. In the subsequent decades, the HSST Program conducted extensive large-scale experiments with intermediate-size vessels (with varying size flaws) pressurized to failure, similar experiments under conditions of thermal shock and even pressurized thermal shock (PTS), wide-plate crack-arrest tests, and biaxial tests with cruciform-shaped specimens. Extensive analytical and numerical studies accompanied these experiments, including the development of computer codes such as the recent Fracture Analysis of Vessels Oak Ridge code currently being used for PTS evaluations. In the absence of radiation damage to the RPVs, fracture of the vessel is improbable. However, it was recognized that exposure to high energy neutrons can result in embrittlement of radiation-sensitive RPV materials. The HSSI Program conducted a series of experiments to assess the effects of neutron irradiation on RPV material behavior, especially fracture toughness. These studies included RPV plates and welds, varying chemical compositions, and fracture toughness specimens up to 101.6 mm (4 in.) thickness. The results of these investigations, in conjunction with results from commercial reactor surveillance programs, are used to develop a methodology for the prediction of radiation effects on RPV materials. Results from the HSST and HSSI programs are used by the USNRC in the evaluation of RPV integrity and regulation of overall nuclear plant safety.

A new calculation on spectrum of direct DNA damage induced by low-energy electrons

In this work, direct DNA damage induced by low-energy electrons (<5 keV) is simulated using Monte Carlo methods, and the resulting yield of various strand breaks and base damages in cellular environment is presented. The simulation is based on a new inelastic cross section for the production of electron track structure in liquid water, and on ionization cross sections of DNA bases to generate base radical. Especially, a systematic approach of simulating detailed base damage is suggested. This approach includes improvement of a volume model of DNA, generation of the DNA base sequence, conversion of ionization events in liquid water at hit site to the ionization interaction of electrons with DNA bases and development of an algorithm to convert a base radical to a damage. The results obtained in terms of strand breaks are compared with those of experiments and other theoretical calculations, and good agreement was obtained. The yield of detailed base damages and clustered DNA damages caused by the combination of various strand breaks and base damages is presented, and the corresponding distribution characteristics are analyzed. The influence of the relative content of base pairs A-T and G-C in a DNA segment on the yield of both strand breaks and base damages is also explored. The present work provides fundamental information on DNA damage and represents the first effort toward the goal of obtaining the spectrum of clustered DNA damage including detailed base damages, for the mechanistic interpretation and prediction of radiation effects.

Blood and small intestine cell kinetics under radiation exposures: Mathematical modeling

Mathematical models which describe the dynamics of two vital body systems (hematopoiesis and small intestinal epithelium) in mammals exposed to acute and chronic radiation are developed. These models, based on conventional biological theories, are implemented as systems of nonlinear differential equations. Their variables and constant parameters have clear biological meaning, that provides successful identification and verification of the models in hand. It is shown that the predictions of the models qualitatively and quantitatively agree with the respective experimental data for small laboratory animals (mice, rats) exposed to acute/chronic irradiation in wide ranges of doses and dose rates. The explanation of a number of radiobiological effects, including those of the low-level long-term exposures, is proposed proceeding from the modeling results. All this bears witness to the validity of employment of the developed models, after a proper identification, in investigation and prediction of radiation effects on the hematopoietic and small intestinal epithelium systems in various mammalian species, including humans. In particular, the models can be used for estimating effects of irradiation on astronauts in the long-term space missions, such as Lunar colonies and Mars voyages.