Rapid urbanization and increased human activity have negatively impacted the microclimate of cities, leading to unfavorable conditions for human thermal comfort, particularly in outdoor spaces. Thermal comfort can be improved through various means, such as adjusting the height of urban buildings, the aspect ratio of road widths, and the placement of street trees. This study employed the ENVI-met software V5.5.1 to simulate the microclimate based on aspect ratio (H/W = 1.5) and street tree spacing (6 m) similar to actual conditions with different aspect ratios (H/W = 0.5, 1.0, and 2.0) and street tree spacing (2 m) in Seoul, Republic of Korea. Thermal comfort was assessed through a comprehensive predicted mean vote (PMV) evaluation, considering the gender (male and female) and age (8, 35, and 80 years) of residents in the target area, to determine the optimal urban canyon scenario. The results of the study indicated that the height of the building and the percentage of trees had a significant impact on the temperature and PMV results. When comparing PMV results, women have higher thermal vulnerability than men, and based on age, older adults have higher thermal vulnerability. The aspect ratio of 1.5 and tree spacing of 2 m resulted in the lowest temperature of 35.91 °C at 12:00 p.m. at 0° wind direction and 36.09 °C at 90° wind direction, lower than the actual input value of 36.9 °C. The PMV values were also under the same conditions, with an average PMV by gender of 3.87 at 0° and 4.21 at 90° and an average PMV by age of 3.86 at 0° and 4.19 at 90°. This finding is significant because it can inform the development of planned cities that prioritize urban thermal comfort during summer. This can be achieved through the strategic design of urban canyons and incorporation of street trees in both new and existing cities.