To improve the living and sleeping quality for human beings, air conditioning is widely applied to maintain a comfortable indoor environment in bedrooms. However, most current air conditioning methods in bedrooms are based on mixing ventilation, which is low efficiency due to the uniform environment created by this ventilation method. In this study, stratum ventilation is introduced applied in a sleeping environment to improve ventilation performance. To better analyze the application potential of stratum ventilation, three ventilation methods are compared, including mixing ventilation, displacement ventilation and stratum ventilation. Based on computational fluid dynamics technology, the energy and exergy efficiency of the three air distribution methods are conducted and compared. The results show that stratum ventilation provides a nonuniform environment both in the vertical and horizontal directions in a typical sleeping environment with a higher energy and exergy efficiency than the mixing ventilation and displacement ventilation. When the predicted mean vote value in the occupied zone equals zero, energy consumption and exergy loss of stratum ventilation reduce 10% and 15.6% in comparison with mixing ventilation respectively. The energy consumption difference of stratum ventilation and displacement ventilation is not significant, but the exergy loss reduces 6.2%, with the predicted mean vote equals zero in the occupied zone. In addition, the energy utilization coefficients of stratum ventilation, displacement ventilation and mixing ventilation are 1.3, 1.03 and 0.89 respectively. The results show that stratum ventilation has a high potential for energy-saving in a sleeping environment.