Ppm BAP Research Articles

Effect of BAP concentration on the growth of several types of rambutan plants (Nephelium lappaceum L.) in vitro

Abstract In vitro seed propagation is needed as an alternative to providing superior rambutan planting material. This research aimed to examine the effect of BAP on the growth of several varieties of rambutan in vitro. The method used was a completely randomized design with 2 factors, the first factor was rambutan varieties (binjai, rapiah, and ace), and the second factor was BAP concentrations (0, 2, 4, and 6 ppm). There were 12 treatment combinations and 3 times replications. Variables observed was analyzed using analysis of variance followed by 5% Duncan’s Multiple Range Test and other data (leaf emergence time, number of leaves, root emergence time, number of roots, and root length) were analyzed descriptively. The results showed that the combination of 0 ppm BAP and binjai variety gave the best results on the shoot length, number of leaves, and number of roots. The 0 ppm BAP treatment gave the best results on the number of shoots, shoot length, number of leaves, number of roots, and root length. Binjai variety treatment gave the best results on the time of shoot emergence, shoot length, time of leaf appearance, number of leaves, time of root appearance, and number of roots.

Induction and multiplication of callus of AS2K clones Coffea arabica L. through 2,4-D and BAP combination

Abstract Arabica coffee (Coffea arabica L.) has dominated international trade with more than 70% of global coffee production because of it’s taste was more preferred than the other types of coffee. The AS2K clone of C. arabica is a superior clone with high productivity, resistance to pests and diseases, and has a good taste. To maintain these characters, the appropriate propagation is by vegetatively. The aim of this research was to induce callus formation from C. arabica leaves, and to multiplicate the callus on twelve (12) combinations of 2,4-D and BAP as an effort to develop a method for propagating Arabica coffee. Callus induction was conducted on Murashige and Skoog (MS) medium containing 2,4-D 2 mg/L combined with BAP 1 mg/l, and then the multiplication of these callus on MS medium with 12 combinations of 2,4-D (2, 3, or 4 mg/L) and BAP (0, 0.25, or 1 mg/L). The research design used a completely randomized design with 3 replications, and each replication contained 5 callus pieces. The results showed that the combination of 2 ppm 2,4-D and 1 ppm BAP was able to induce callus formation from leaf explants by 49% at week 3 culture, yellowish white in color and friable in texture. Combination of 2,4-D 3 mg/L and BAP 0.5 mg/L provide the best result for callus multiplication.

Callus formation response from immature male flower explant of plantain banana (Musa acuminata x Musa balbisiana cv. Kepok) treated by 2,4-D and BAP

Callus induction is an important step in indirect somatic embryogenesis. The aim of this study was to obtain an optimal medium for the callus formation of plantain bananas (cv. Kepok). The research was conducted from May to August 2022 at the Tissue Culture Laboratory of PT. ITCI Kartika Utama, Penajam Paser Utara District, East Kalimantan. The explant used an immature male flower of plantain banana (cv. Kepok). The experiment used a completely randomized design with two factors concentration of 2,4-D (1,2 and 4 ppm) and BAP (5, 10, and 15 ppm). The appearance of callus was monitored daily until it developed explants. On the final observation day, the percentage of explants with callus, as well as the color and texture of the callus, were assessed. The research findings suggest that the optimal medium for inducing callus in male banana flower explants was MS medium supplemented with 2 ppm 2,4-D and 5 ppm BAP. This medium resulted in calluses that exhibited a yellowish-white color and a compact texture. Keywords: callus morphology; genetic improvement; plant growth regulator; tissue culture; triploid banana

Pengaruh Kombinasi BAP (Benzyl Amino Purine) dan Kinetin Terhadap Multiplikasi Tunas Tanaman Tebu (Saccharum officinarum L.) Varietas AAS Agribun

Sugarcane (Saccharum officinarum L.) is a plantation commodity crop and raw material for making sugar. The need for sugar increases every year so it is necessary to provide superior sugarcane seeds in large quantities in a relatively short time. This can be done by propagating using the plant tissue culture method. The research method used was an experimental method using a combined Completely Randomized Design (CRD) with 4 replications and 8 treatments consisting of: Control (P0); 0.5 ppm BAP + 0 ppm kinetin (P1); 1 ppm BAP + 0 ppm kinetin (P2); 2 ppm BAP + 0 ppm kinetin (P3); 0 ppm BAP + 0.1 ppm kinetin (P4); 0.5 ppm BAP + 0.1 ppm kinetin (P5); 1 ppm BAP + 0.1 ppm kinetin (P6); and 2 ppm BAP + 0.1 ppm kinetin (P7). The data then explained the variations and then continued with the DMRT test at the 5% level. The results showed that treatments P3 and P7 showed the best results at the time of shoot emergence. The P3 treatment also showed the best results in the number of shoot (8.25) and number of leaves (16.50). The P7 treatment showed the highest shoot yield (4.58). Keywords: sugarcane, BAP, kinetin, shoot multiplication

Induksi Kalus dari Hipokotil Belimbing Merah (Baccaurea angulata) dengan Penambahan 2,4-D (Dichlorophenoxy Acetic Acid) dan BAP (6-Benzyl Amino Purin)

Red starfruit is one of the endemic plants of Kalimantan which produces secondary metabolites which has been empirically used as antioxidant, antibacterial, and anticancer. This plant has a long reproductive period and seedless fruit is often found during the fruiting season, which is a problem for the sustainability of the red starfruit in nature. Conservation of red star fruit for propagation and production of secondary metabolites can be done in vitro through callus culture. This research aims to determine the effect of adding the growth regulators dichlorophenoxy acetic acid (2,4-D) and 6-benzyl amino purine (BAP) on callus induction time, percentage of callus forming explants, callus color and texture, and callus growth. This research used a Completely Randomized Design (CRD) with a factorial pattern consisting of two treatment factors, namely 2,4-D (0; 0.25; 0.5; 0.75; 1 ppm) and BAP (0; 0.25; 0.5; 0.75; 1 ppm). The percentage of explants forming callus (%), the time the callus appeared (days after planting), the color and texture of the callus, the wet weight and dry weight of the callus (g) were research observation parameters.The results showed that the single factor 2,4-D, single factor BAP, and the interaction of 2,4-D and BAP had a significant effect to callus appearance time. Single administration of 2,4-D and BAP had a significant effect on callus dry weight. The color variations of the callus produced are white, green and brown with a compact texture. The fastest callus emergence time was at 11.00 days after planting (DAP), namely in the combination treatment of 1 ppm 2,4-D and 0.75 ppm BAP, single treatment 0.75 ppm and 1 ppm 2,4- D. The highest average dry weight of 0.024 g was obtained in the single treatment of 0.75 ppm BAP.

Conservation implications of seed germination studies of Aquilegia fragrans Benth.: A native and endangered medicinal plant species of Kashmir Himalaya

Insufficient investigation of seed germination studies of threatened plant species creates practical obstacles to their conservation efforts. Here, we investigate the seed germination of Aquilegia fragrans Benth.-an endangered medicinal plant species native to Himalaya. The effects of stratification conditions including time (10, 20, 30, and 40 days), temperature (−4, −10, and −20 °C), and hormone treatments (250 ppm GA3, 30 ppm BAP, and 30 ppm Zeatin) on germination of A. fragrans seeds collected from the alpine belt of Kashmir Himalaya were examined. Seeds of species exhibited non-deep simple morphophysiological dormancy (MPD). Seed germination showed significant differences among the various treatments. It was observed that the application of GA3, zeatin, and BAP significantly improved seed germination as compared with the control. The highest germination percentage (73 ± 1 %) along with maximum germination index (1.05 ± 0.05) was recorded in T30 (Seeds stratified for 30 days at −10 °C and treatment with Zeatin 30 ppm) followed by T19 (seeds stratified at −10 °C for 20 days and treatment with zeatin 30 ppm). The seed germination studies of A. fragrans have not been carried out before, and the information gathered in the present study may prove useful for the conservation of the target plant species. The present study will help in the conservation, sustainable development, and restoration of this prized medicinal plant species.

Propagação rápida da planta ornamental Homalomena gigantea usando estacas de rizoma

Abstract This study investigates the critical factors influencing shoot and root regeneration from Homalomena gigantea rhizome cuttings, providing valuable insights for rhizome cutting practices for this plant species. Three key experiments were conducted to assess the impact of plant growth regulator (PGR) concentrations, cutting length, and cutting section on regeneration. In the first experiment, different concentrations of α-NAA and BAP were examined, revealing that PGR selection and concentration play a pivotal role in shoot and root induction and plantlet development. The highest success rates were observed at 150 ppm α-NAA and 100 ppm BAP, although there was a trade-off between the quantity and quality of plantlets, emphasizing the need for a balanced plant regulator selection. The second experiment investigated the influence of rhizome cutting length. Longer cuttings, particularly at 5 and 7 cm, consistently led to better shoot and root development, highlighting the importance of selecting appropriate cutting lengths. In the third experiment, cutting sections (apical, middle, and distal) without PGR concentrations were assessed, with all sections showing high regeneration rates. However, the apical section produced the highest-quality plantlets with superior growth parameters, followed by the middle section, while the distal section exhibited lower effectiveness. In summary, these findings underscore the significance of PGR choice and concentration, cutting length, and cutting section for successful regeneration of H. gigantea from rhizome cuttings. These insights provide valuable guidance for enhancing rhizome cuttings methods for this plant species and promoting efficient propagation.

Effect of Varied Levels of Gibberellin, Anti-Gibberellin and Cytokinin on Growth and Tuberization in Potato

Plant hormones play a significant role in potato tuberization. While Gibberellins inhibit tuberization, anti-gibberellins (PBZ and CCC) and cytokinins promote tuberization in potato. The experiment demonstrated the clear roles of these hormones in facilitating tuberization in potato. Treatment with 25 ppm GA significantly increased plant height (44.56 cm) compared to the control (32.22 cm), while CCC, a Gibberellins (GA) inhibitor, reduced the plant height by inhibiting GA-induced stem elongation. Hormonal treatments affected the number of nodes, leaves, branches, SPAD readings, stem girth, root length and root weight with significantly higher values observed compared to the control. 50 ppm GA showed the highest number of stolons per plant (6.33), but surprisingly resulted in a few tubers per plant (1.7) compared to the control (1.8). However, both 5 ppm and 10 ppm BAP led to significantly more tubers per plant (2.1 and 3.2 respectively), as did 5 ppm and 10 ppm Kinetin (2.8 and 2.6 respectively). Regarding tuber fresh weight, all treatments except CCC and higher concentration of kinetin showed significantly higher fresh weight compared to control. In summary, 50 ppm Gibberellins increased the stolon number, while 10 ppm BAP increased the tuber number and fresh weight in potato

Pengaruh waktu induksi dan ZPT terhadap pertumbuhan kalus kotiledon kacang panjang (Vigna unguiculata subsp. sesquipedalis)

Yard-Long bean plants are an agricultural commodity that has great potential to be developed because they are easy to cultivate, their market share is also quite high but there are still problems in meeting the need for sufficient seeds and they are still susceptible to pests and disease. Therefore, efforts need to be made to increase production and induce variations. One method that can be used is tissue culture for the induction of somaclonal variations and/or the formation of transgenic plants. This study aims to determine the effect of variations in callus initiation time and the effect of Plant Growth Regulators (PGR) administration on the growth of long bean cotyledon explant callus. Seed sterilization was carried out using detergent, 10% sodium hypochlorite, 70% alcohol and sterile distilled water. The results of the research showed that variations in callus initiation time on MS media with the addition of 2,4-D PGR and the use of several variations of PGR in subcultures were able to initiate callus and influence callus size, callus color, and the percentage of browning of Yard-long bean cotyledon explants. Callus initiation time of 4 weeks with a combination of 2 ppm BAP concentration with 0.1 ppm IAA produced the largest callus size and brownish white callus color. The addition of PGR during subculture had a smaller browning percentage than the treatment without PGR.

The Effect of BAP and NAA Combination on Callus Induction of Aglaonema Siam Aurora Leaf Explants in Vitro

This research aims to determine the effect of the Naphthalene Acetic Acid and Benzyl Amino Purine combination on callus growth of Aglaonema Siam Aurora leaf explants and to determine the combination level at the most optimal concentration on the callus growth of Aglaonema Siam Aurora leaf explants. Observation variables included callus emergence time, explants percentage of formed callus, callus length, and explant life percentage. The explants that where used were the first young leaves explants from shoots that were opened, with 2x2 cut size. Results show that the addition of a NAA and BAP combination had an effect on . Combination of 1.2 ppm BAP + 1 ppm NAA treatment is the most optimum treatment for callus growth with a callus percentage of 87.5%, the callus start time at week 3, the average callus length is 0.77 mm and the percentage number of survived explants is 100%

In vitro Culture Optimization of Pomelo Seeds (Citrus maxima (Burm.) Merr.): A South Sulawesi Orange.

<b>Background and Objective:</b> Indonesia boasts a variety of delicious tropical fruits, including pomelo, mainly grown in Pangkep Regency, South Sulawesi Province. However, in this region, some challenges hinder such as inadequate care, aging trees and limited seed supply hinder productivity in this region. <i>In vitro</i> culture methods present a solution by rapidly producing high quality, disease-free pomelo seeds. This study aims to determine the optimal concentration of the BAP added to the culture medium to induce shoots from pomelo seeds. <b>Materials and Methods:</b> The seeds were planted on MS media with the addition of BAP hormone (0.5, 1, 1.5, 2 and 2.5 ppm) and 0 ppm as the control. The experimental units were arranged in a CRD and analyzed using SPSS 20.0 software, employing the Shapiro-Wilk normality test and Levene's Statistic for homogeneity. If the data met the normality and homogeneity assumptions, ANOVA was applied, followed by the DMRT for a parametric test. Otherwise, a non-parametric test namely the Kruskal-Wallis was conducted and differences were further analyzed using the Mann-Whitney test at a 5% significance level. <b>Results:</b> The application of the BAP accelerated shoot emergence, with the most rapid development occurring on the 10th day after planting (DAP), at a BAP concentration of 2.5 ppm for red pomelo. For white pomelo and sweet pomelo, shoots appeared on the 19th and 20th days, respectively at a 2 ppm BAP. Interestingly, root development was fastest between the 4th and 6th DAP in 0 ppm BAP (control). <b>Conclusion:</b> The addition of the BAP at a concentration of 1.5 ppm in the culture medium promotes faster shoot emergence and has a significant impact on the number of shoots in red pomelo.

Induksi Tunas Karamunting (Rhodomyrtus tomentosa) secara Invitro

Tissue culture techniques provide an alternative to vegetative plant propagation efforts in conservation efforts for Karamunting, the existence of which is becoming increasingly rare. The explant as the material used is the result of in vitro germination using several concentrations of GA3. This research was structured in the form of a factorial experiment with a completely randomized factorial design (RAL) with 2 factors. The first factor is the BAP concentration with three levels, namely B1 = BAP 0.5 B2 = BAP1 ppm and B3 = BAP 1.5 ppm. The second factor is the concentration of TDZ with three levels, namely T1 = TDZ 0 ppm, T2 = TDZ 0.25 ppm and T3 = TDZ 0.5 ppm. The results of the research showed that there was an interaction between the growth regulators BAP and TDZ on the percentage of live explants and the height of karamunting explants. In terms of the percentage of live explants, giving BAP 0.50 ppm without TDZ is the best concentration with a live percentage of 100%. Treatment of 1 ppm BAP and 0.25 ppm TDZ produced the highest shoots, namely 2.83 mm. The time of emergence of shoots and the number of shoots per explant had no interaction and the influence of a single factor, several concentrations of BAP and TDZ.

Application of BAP and IBA for In Vitro Callus Formation of Biduri (Calotropis gigantea)

A herbaceous plant called biduri (Calotropis gigantea) is useful as a medicine. The leaves, roots, and flowers are just a few of the organs that contain secondary metabolites. A callus is an accumulation of secondary metabolite-producing amorphous cells. Through the induction of plant growth regulators, callus formation can be induced. The goal of the study was to find the BAP and IBA concentration that would promote callus formation in biduri. The initialstem segments of biduri, which were 1.5 months old and 2 cm long, were used as explants. A factorial design using two components and three repetitions of each was employed in a fully randomized design. BAP concentrations (0, 1, 2, 3, 4 ppm) and IBA concentrations were the first and second factors, respectively (0, 1, 2, 3, 4 ppm). The callus induction time, callus morphology (color and texture), and callus appearance percentage were the factors that were observed. The results demonstrated that a green, compact callus could be produced at a concentration of 4 ppm BAP and 1 ppm IBA. The proportion of callus manifestation rose and the callus induction time decreased with an increase in BAP concentration of up to 4 ppm.

The Effect of PVP (Polivinil Pirolidon) and BAP (6-benzylamino purine) on Shoots Induction of Teak Plus Perhutani (Tectona grandis)

The goal of this study is to see how BAP and PVP concentrations affect in vitro teak shoot induction. The research was carried out at Diponegoro University’s Tissue Culture Laboratory, Faculty of Animal Husbandry and Agriculture, between April and June 2022. The research method was a 4x4 factorial design based on a completely randomized design (CRD) with three replications. The first factor was the concentration of PVP (mg.L-1): 0, 110, 220, and 330, and the second was the concentration of BAP (ppm): 0, 1.3, 2.6, and 3.9. Duncan’s Multiple Range Test (DMRT) and orthogonal polynomials were used to test the data for variance. The interaction between the 0 mg.L-1 PVP treatment and the 110, 220, and 330 mg.L-1 PVP treatments after the addition of 2.6 ppm BAP had the quickest callus emergence time, with an average of 7 days. PVP treatments of 110 mg.L-1 and 220 mg.L-1 had a significant effect on the shoot length parameter. BAP concentrations of 1.3 and 2.6 ppm were found to increase the shoot length, number of shoots, and number of leaves response parameters. The 1.3 ppm BAP treatment also reduced browning by 17% in the explants. There was no effect of PVP or BAP treatments on shoot emergence time, leaf emergence time, or the percentage of explants that formed shoots.

Shoots and Roots Induction of Garlic on Different Composition of Plant Growth Regulators and Photoperiod

Plant Growth Regulators and photoperiods will affect callus morphogenesis from garlic bulbils. The study aimed to obtain the best interaction between media composition and photoperiod for shoots and root induction of the garlic callus. The research has two-stage. The first stage consisted of two factors. The first factor is the combination of PGR for shoots induction which consists of three combinations, i.e., 0.1 ppm NAA + 1 ppm BAP, 2 ppm NAA + 2 ppm BAP, and 0.4 ppm 2,4-D + 2 ppm kinetin. The second factor is photoperiod which consists of 10, 16, and 22-h light conditions. The second stage is the root induction in different PGRs (2 ppm IBA and 0.2 ppm NAA + 2.25 ppm BA) under continuous dark conditions. The results of the first stage showed that the PGR produced the most shoots was 0.1 ppm NAA + 1 ppm BAP. After four weeks, the frequency of an explant emerging during the shoot reached 64% on media 0.1 ppm NAA + 1 ppm BAP. The treatment MS media supplemented with 0.1 ppm NAA + 1 ppm BAP at 16-h photoperiods increased the growth of shoots per explant in 11-15 shoots and produced the most extended shoot length. The result of the second stage showed that the number of roots on 2 ppm IBA is the same as NAA 0,2 ppm + BA 2,25 ppm, but the treatment 2 ppm IBA has the highest root number in three months.

Propagation of Gaharu Plant Gyrinops Versteegii Species Provenant Beringin Throught in Vitro Culture

Grynops verstegii is a non-timber forest product commodity that has a very high economic value. People know this tree as the agarwood tree. In Lombok, the Beringin provenance is one of the leading provenances, so it is very important to conserve its germplasm. This study aims to find alternatives for gaharu development through seed production using in vitro culture techniques. This research was carried out in July 2021 at the Tissue Culture Unit of the Immunobiology Laboratory, University of Mataram. In this experiment there were 8 media treatments with different concentrations of BAP (Ssitokini) and NAA (auxin). The results showed that callus growth occurred on MS 1, MS 3, MS 4, MS 6, and MS 7 media. However, the best growth was obtained in MS 1 treatment with a combination of 1.0 ppm BAP and 0.5 ppm NAA. While most of the samples experienced browning and contamination.

Perlakuan Air Kelapa Tua, Dan BAP Pada Media MS, VW Terhadap Protocorm Anggrek Dendrobium sp. Secara Kultur In vitro

This study aims to obtain accurate results from the concentration of old coconut water and BAP on Vacin and Went and Murashige and Skoog media on the growth of Dendrobium sp. through in vitro culture. The method used in this study was a completely randomized design consisting of 8 treatments and 8 replications. Each replication consisted of 2 bottles so that a total of 128 bottles, namely A0 (control, without using old coconut water), A1 (12.5% old coconut water), B0 (control, without using BAP), B1 (BAP 0.5 mg/L ), and used 2 types of media (Murashige and Skoog) and (Vacin and Went). The variables observed included the number of protocorm, wet weight, dry weight, and pH of the media. The results showed that the concentration of old coconut water and BAP had a significant effect on the number of protocorm, fresh weight, and pH of the media on Dendrobium sp. The best treatment obtained was VA1B1 (12.5% old coconut water, 0.5 ppm BAP) with a media pH of 5.0 V A1B1, the number of protocorm V A1B1 49.57, and VA1B1 0.008 g which had the best results compared to other treatments.

Induksi dan Multiplikasi Kalus Eucalyptus sp. pada Berbagai Media Callus Induction Medium (CIM) Secara In Vitro

Callus Induction and Multiplication of Eucalyptus sp. on Various Media Callus Induction Medium (CIM) In Vitro. Callus induction in vitro can be done by planting specific part of plant such as leaf, root, stem, etc in aseptic condition by using medium that contains 2,4-D, a plat regulator for promoting callus formation. One of the objectives of callus production in vitro is to form secondary metabolites, because eucalyptus is known as a producer of essential oils. This research is an initial study for in vitro production of secondary metabolites for further research. The aim of this study was to determine the most suitable callus induction medium for callus formation and callus multiplication for Eucalyptus grown in vitro. This research was designed as a Completely Randomized Design (CRD), five treatments, repeated five times. Woody Plant Medium (WPM) was used as the basic media. Several Callus induction Medium (CIM) were used as treatments, i.e. CIM1 = 1 ppm 2,4-D; CIM2 = 1,5 ppm 2,4-D; CIM3 = 2 ppm 2,4-D; CIM4 = 1 ppm NAA+1 ppm BAP; and CIM5 = 2 ppm NAA+ 2 ppm BAP. Variables observed were the time of curly leaf formation, time of the emerging callus, colour and texture of the callus, callus’s weight, callus surface area, the weight of callus after multiplication. The result showed that CIM4 and CIM5 had the fastest emerging callus (7 days after planting), CIM1 and CIM2 formed green and crumb texture of callus, CIM1 produced the highest of callus’s weight, and CIM2 resulted in the highest of callus’s surface area. In conclusion, the most suitable CIM for callus induction was CIM1, while for callus multiplication was CM2.

Inducing potato tuber formation at low elevation of tropical region by foliar spray of PGR mixtures at different application times

Growing potato crops at low elevation in the tropical region may face many problems related to high temperature, such as an increase in endogen GA3 levels, causing the inhibition of tuber formation. Suppressing GA3 biosynthesis, by applying a mixture of plant growth regulators (PGRs), is of important. The objectives of this experiment were to study the best PGR mixtures, PGR application time, and their interaction in promoting tuber formation at low altitude of tropical areas in Indonesia. The experiment used a completely randomized design arranged in factorial (2 factors, 3 replications). The first factor was a mix of plant growth regulator solution (M), consisting of 4 levels, which were: M0 = No PGR; M1 = 10 ppm 2,4-D + 100 ppm Daminozide, M2 = M1 +10 ppm BAP, M3 = M1 + 25 ppm COU, and M4 = M1+ 500 ppm PBZ. The second factor was application time of the solutions (T), consisting of 3 levels, which were: T1 = 3, 4, 5, 6 weeks after emergence (WAE), T2 = 3, 4, 5 WAE, and T3 = 3, 4 WAE. The results showed that all of the PGR mixtures promoted tuber formation with 10 ppm 2,4-D + 100 ppm Daminozide + 500 ppm Paclobutrazol was found as the best PGR mixture. Moreover, the best time to spray the mixture was 3,4,5,6 WAE. Finally, the best interaction between PGR and application time was10 ppm 2,4-D + 100 ppm Daminozide + 10 ppm BAP applied at 3,4,5 WAE.

In Vitro Multiplication of Nepenthes mirabilis Lour (Druce) with Variations Concentration of Sucrose and BAP

Nepenthes mirabilis (Lour) Druce is a rare ornamental plant and has high economic value so it needs to be preserved. This study aimed to analyze effect of sucrose and BAP concentrations and their optimal interactions in the in vitro multiplication of Nepenthes mirabilis. Explants in the form of in vitro plantlets from Laboratory of Plant Tissue Culture, Department of Biology, Semarang State University. The treatment was carried out by adding sucrose and BAP to murashige skoog (MS) media with various concentrations. The treatments were arranged in two factor completely randomized design with four replications. Plantlets were planted in the treatment medium for 10 weeks, then every week the growth of shoots and leaves of N. mirabilis was observed. The data were analyzed by two-way analysis of variance and Duncan's test. The results showed that concentration of sucrose, BAP, and their interactions affected the growth of explants. Plantlets grown on media with sucrose concentration 20 g/l produced the highest number of shoots, media with concentrations BAP 3 and 2 ppm produced the highest number of shoots and number of leaves. Medium ½ MS with varying concentrations of sucrose 20 g/l with 3 ppm BAP and sucrose 20 g/l with 2 ppm BAP resulted in the highest growth in the number of shoots and number of leaves. The composition of this medium can be used for the multiplication of N. mirabilis.