The Effect of PVP (Polivinil Pirolidon) and BAP (6-benzylamino purine) on Shoots Induction of Teak Plus Perhutani (Tectona grandis)

Abstract

The goal of this study is to see how BAP and PVP concentrations affect in vitro teak shoot induction. The research was carried out at Diponegoro University’s Tissue Culture Laboratory, Faculty of Animal Husbandry and Agriculture, between April and June 2022. The research method was a 4x4 factorial design based on a completely randomized design (CRD) with three replications. The first factor was the concentration of PVP (mg.L-1): 0, 110, 220, and 330, and the second was the concentration of BAP (ppm): 0, 1.3, 2.6, and 3.9. Duncan’s Multiple Range Test (DMRT) and orthogonal polynomials were used to test the data for variance. The interaction between the 0 mg.L-1 PVP treatment and the 110, 220, and 330 mg.L-1 PVP treatments after the addition of 2.6 ppm BAP had the quickest callus emergence time, with an average of 7 days. PVP treatments of 110 mg.L-1 and 220 mg.L-1 had a significant effect on the shoot length parameter. BAP concentrations of 1.3 and 2.6 ppm were found to increase the shoot length, number of shoots, and number of leaves response parameters. The 1.3 ppm BAP treatment also reduced browning by 17% in the explants. There was no effect of PVP or BAP treatments on shoot emergence time, leaf emergence time, or the percentage of explants that formed shoots.