Power Stroke Research Articles

Numerical Analysis of a Liquid Nitrogen (LN2) Engine for Efficient Energy Conversion

A liquid fuel that produces no toxic exhaust could help reduce pollution, potentially in urban areas. In this study, a simulation was conducted using the AVL Boost platform, on the use of liquid nitrogen (LN2) in a four-stroke engine. This study is focused on engine performance using directly introduced LN2 and the analysis of related aspects (inlet, outlet, and in-cylinder pressure, temperature, conditions for LN2 evaporation, etc.) that indicate the possible potential for the development of a zero-emission direct injection internal evaporation (DI-IE) LN2 engine. AVL Boost software was uniquely customized to accommodate the simulations, as modeling with LN2 was not available in the standard features. Simulation results, including indicated mean effective pressure (IMEP), effective torque, and power, were compared with similarly sized diesel and gasoline engines running at speeds of up to 1000 rpm. The LN2 injection mass was matched with air intake to evaluate the optimal combination. The simulation results showed that the enthalpy of the aspirated air was sufficient to evaporate and expand the injected amount of LN2 in each cycle, generating the in-cylinder pressure for the power stroke. The IMEP of the LN2 engine was similar to internal combustion engines, and its indicated efficiency was about four times higher (56–62%). The air separation process was 44% efficient in producing the required LN2, making the overall efficiency about 31%.

A reverse stroke characterizes the force generation of cardiac myofilaments, leading to an understanding of heart function

Changes in the molecular properties of cardiac myosin strongly affect the interactions of myosin with actin that result in cardiac contraction and relaxation. However, it remains unclear how myosin molecules work together in cardiac myofilaments and which properties of the individual myosin molecules impact force production to drive cardiac contractility. Here, we measured the force production of cardiac myofilaments using optical tweezers. The measurements revealed that stepwise force generation was associated with a higher frequency of backward steps at lower loads and higher stall forces than those of fast skeletal myofilaments. To understand these unique collective behaviors of cardiac myosin, the dynamic responses of single cardiac and fast skeletal myosin molecules, interacting with actin filaments, were evaluated under load. The cardiac myosin molecules switched among three distinct conformational positions, ranging from pre- to post-power stroke positions, in 1 mM ADP and 0 to 10 mM phosphate solution. In contrast to cardiac myosin, fast skeletal myosin stayed primarily in the post-power stroke position, suggesting that cardiac myosin executes the reverse stroke more frequently than fast skeletal myosin. To elucidate how the reverse stroke affects the force production of myofilaments and possibly heart function, a simulation model was developed that combines the results from the single-molecule and myofilament experiments. The results of this model suggest that the reversal of the cardiac myosin power stroke may be key to characterizing the force output of cardiac myosin ensembles and possibly to facilitating heart contractions.

Metachronal Swimming of Mantis Shrimp: Kinematics and Interpleopod Vortex Interactions.

Mantis shrimp swim via metachronal rowing, a pattern in which the pleopods (swimming limbs) stroke sequentially, starting with the last pair and followed by anterior neighbors. A similar swimming pattern is used at various sizes, Reynolds numbers, and advance ratios by diverse organisms including ciliates, ctenophores, copepods, krill, and lobsters. Understanding this type of locomotion is important because it is widespread and may inspire the design of underwater vehicles where efficiency, robustness, and maneuverability are desired. However, detailed measurements of the flow around free-swimming, metachronally rowing organisms are scarce, especially for organisms swimming in a high Reynolds number regime (Re ≥ 104). In this study, we present time-resolved, planar PIV measurements of a swimming peacock mantis shrimp (Odontodactylus scyllarus). Simultaneous kinematics measurements of the animal, which had body and pleopod lengths of 114 and 20 mm, respectively, reveal mean swimming speeds of 0.2-1.9 m s-1 and pleopod beat frequencies of 3.6-13 Hz, corresponding to advance ratios of 0.75-1.84 and body-based Reynolds numbers of 23,000-217,000. Further, the animal's stroke is not purely metachronal, with a long phase lag between initiation of the first and fifth pleopod power strokes. Flow measurements in the sagittal plane show that each stroking pleopod pair creates a posteriorly moving tip vortex which evades destruction by the recovery strokes of other pleopod pairs. The vortex created by the anteriormost pleopod pair is the strongest and, owing to the animal's high advance ratio, is intercepted by the power stroke of the posteriormost pleopod pair. The vortex strength increases as a result of this interaction, which may increase swimming speed or efficiency. A relationship for vortex interception by the posterior pleopod is proposed that relates the phase lag between the interacting pleopods to the beat frequency, distance between those pleopods, and speed of the vortex relative to the animal. We describe this interaction with a novel parameter called the interpleopod vortex phase matching Strouhal number StIVPM which is equal to the phase lag between interacting pleopods. This new nondimensional parameter may be useful in predicting the conditions where a constructive interaction may occur in other species or in physical models. Finally, we relate the advance ratio to the Reynolds number ratio, the ratio between the body-based Reynolds number and the pleopod-based Reynolds number. The importance of these parameters in promoting the interpleopod vortex interactions identified here, in dynamically scaled experiments, and in wake signatures behind schooling metachronal swimmers is discussed.

Evolution of mechanical cooperativity among myosin II motors

Myosin II is a biomolecular machine that is responsible for muscle contraction. Myosin II motors act cooperatively: during muscle contraction, multiple motors bind to a single actin filament and pull it against an external load, like people pulling on a rope in a tug-of-war. We model the dynamics of actomyosin filaments in order to study the evolution of motor-motor cooperativity. We find that filament backsliding-the distance an actin slides backward when a motor at the end of its cycle releases-is central to the speed and efficiency of muscle contraction. Our model predicts that this backsliding has been reduced through evolutionary adaptations to the motor's binding propensity, the strength of the motor's power stroke, and the force dependence of the motor's release from actin. These properties optimize the collective action of myosin II motors, which is not a simple sum of individual motor actions. The model also shows that these evolutionary variables can explain the speed-efficiency trade-off observed across different muscle tissues. This is an example of how evolution can tune the microscopic properties of individual proteins in order to optimize complex biological functions.

Metachronal Swimming with Flexible Legs: A Kinematics Analysis of the Midwater Polychaete Tomopteris.

Aquatic animals have developed a wide array of adaptations specific to life underwater, many of which are related to moving in the water column. Different swimming methods have emerged, such as lift-based flapping, drag-based body undulations, and paddling. Patterns occur across scales and taxa, where animals with analogous body features use similar locomotory methods. Metachronal paddling is one such wide-spread propulsion mechanism, occurring in taxa as diverse as ctenophores, crustaceans, and polychaetes. Sequential movement of multiple, near identical appendages, allows for steady swimming through phase-offsets between adjacent propulsors. The soft-bodied, holopelagic polychaete Tomopteris has two rows of segmental appendages (parapodia) positioned on opposite sides along its flexible body that move in a metachronal pattern. The outer one-third of their elongate parapodia consist of two paddle-like pinnules that can be spread or, when contracted, fold together to change the effective width of the appendage. Along with metachronal paddling, tomopterid bodies undulate laterally, and by using high speed video and numerical modeling, we seek to understand how these two behaviors combine to generate effective swimming. We collected animals using deep-diving remotely operated vehicles, and recorded video data in shore- and ship-based imaging laboratories. Kinematics were analyzed using landmark tracking of features in the video data. We determined that parapodia are actively moved to generate thrust and pinnules are actively spread and contracted to create differences in drag between power and recovery strokes. At the same time, the body wave increases the parapodium stroke angle and extends the parapodia into undisturbed water adjacent to the body, enhancing thrust. Based on kinematics measurements used as input to a 1D numerical model of drag-based swimming, we found that spreading of the pinnules during the power stroke provides a significant contribution to propulsion, similar to the contribution provided by the body wave. We conclude that tomopterids combine two different propulsive modes, which are enabled by their flexible body plan. This makes their anatomy and kinematics of interest not only for biologists, but also for soft materials and robotics engineers.

Biomechanical Assessment of the Validity of Sheep as a Preclinical Model for Testing Mandibular Fracture Fixation Devices.

Mandibular fracture fixation and reconstruction are usually performed using titanium plates and screws, however, there is a need to improve current fixation techniques. Animal models represent an important step for the testing of new designs and materials. However, the validity of those preclinical models in terms of implant biomechanics remains largely unknown. In this study, we investigate the biomechanics of the sheep mandible as a preclinical model for testing the mechanical strength of fixation devices and the biomechanical environment induced on mandibular fractures. We aimed to assess the comparability of the biomechanical conditions in the sheep mandible as a preclinical model for human applications of fracture fixation devices and empower analyses of the effect of such defined mechanical conditions on bone healing outcome. We developed 3D finite element models of the human and sheep mandibles simulating physiological muscular loads and three different clenching tasks (intercuspal, incisal, and unilateral). Furthermore, we simulated fractures in the human mandibular body, sheep mandibular body, and sheep mandibular diastema fixated with clinically used titanium miniplates and screws. We compared, at the power stroke of mastication, the biomechanical environment (1) in the healthy mandibular body and (2) at the fracture sites, and (3) the mechanical solicitation of the implants as well as the mechanical conditions for bone healing in such cases. In the healthy mandibles, the sheep mandibular body showed lower mechanical strains compared to the human mandibular body. In the fractured mandibles, strains within a fracture gap in sheep were generally not comparable to humans, while similar or lower mechanical solicitation of the fixation devices was found between the human mandibular body fracture and the sheep mandibular diastema fracture scenarios. We, therefore, conclude that the mechanical environments of mandibular fractures in humans and sheep differ and our analyses suggest that the sheep mandibular bone should be carefully re-considered as a model system to study the effect of fixation devices on the healing outcome. In our analyses, the sheep mandibular diastema showed similar mechanical conditions for fracture fixation devices to those in humans.

Design and thermal analysis of six stroke engine

Abstract The increasing demands in modern combustion engines for low emissions and low fuel consumption improved methods for the cycle of combustion. The Beare Heads is a recent 6-hour version of the Beare Heads, recognized also as Malcolm Beare model. Beare Heads uses pistons and harbours such as a 2-hour motor and replacements for the overlap valve system of 4-hour motors. The four-stroke engine, block as well as shaft remain intact. The six-time engine was a mix of two-hit and four-hit technologies. Six Stroke is a six-stroke engine that uses two energy strokes. The six-time engine including external combustion as well as internal dual stream is the same as the real interior combustion engine, according to its mechanical design. Air heating system as well as a combustion chambers, each separate of the cylinder, are two extra chambers throughout the thermodynamic cycles. The six-stroke is heat efficient, since the power pulse pressure changes are larger than for the injection process as well as compression strokes. The major benefits of six-stroke engines included lowering fuel consumption by 40 percent, reducing emissions, versatility for multi-fuel service by 2 power strokes over the six-stroke cycles.

Inorganic Phosphate Enhances Systolic and Diastolic Function in Excitable Papillary Muscle and Accelerates Myofilament Relaxation in Response to Lengthening

Inorganic phosphate (Pi) is an essential constituent of blood serum found in concentrations of roughly 1.1-1.4 mM. Cardiac myofilament force is reduced with increasing Pi due to its facilitating reversal of the force-producing myosin power stroke. This Pi-dependent reversal of the power stroke is observed to be further augmented by lengthening in demembranated myocardium. We hypothesized that relaxation in excitable myocardium is enhanced by increasing extracellular Pi concentrations and especially when the myocardium is lengthened during the relaxation phase of the force transient. We tested this hypothesis using excitable myocardium isolated from female Wistar rats aged 4-5 months. Rats were fully anesthetized, and their hearts removed. Left ventricular papillary muscles were attached to platinum clips, placed between a force transducer and a length motor at room temperature, and bathed in Krebs solution with 1.8 mM Ca2+ and varying Pi of 0, 1, 2 and 5 mM. Force transients were elicited by electrical stimulation at 1 Hz. Peak tension was statistically unchanged with Pi: 0.641±0.085 mN.mm-2 at 0 mM Pi and 0.749±0.153 mN.mm-2 at 5 mM Pi (mean±SEM, n=9, p=0.46 for repeated measures ANOVA). The maximum rate of tension development (+dP/dt max) normalized to peak tension was significantly enhanced by Pi: 11.0±0.3 s-1 at 0 mM Pi and 13.4±0.5 s-1 at 5 mM Pi (p<0.05). All temporal characteristics of the force transient were significantly shortened with increasing Pi. Time-to-peak was shortened from 162±6 ms at 0 mM Pi to 135±4 ms at 5 mM Pi (p<0.01), and time-to-50% recovery was shortened from 309±12 ms at 0 mM Pi to 258±9 ms at 5 mM Pi (p<0.01). A 1% lengthening stretch with varying duration of 10-200 ms was applied at time-to-50% recovery during the descending phase of the force transient. The recorded force response represented the full viscoelastic response of the activated muscle in late systole/early diastole. Matching lengthening stretches were also applied when the muscle was not stimulated, thus providing a control for the passive viscoelastic response. After subtracting the passive from the active force response, the resulting myofilament response demonstrated features of faster myofilament relaxation in response to the stretch. Temporal characteristics of the resulting myofilament response to stretch were significantly shortened due to the applied lengthening and with increasing Pi. For example, time-to-70% relaxation with 100 ms lengthening duration was shortened by 8.8±6.8 ms at 0 Pi, 19.6±4.8* ms at 1 mM Pi, 31.0±5.6* ms at 2 Pi, and 25.6±5.3* ms at 5 mM Pi (*p<0.01 compared to no change). Our findings suggest that increasing Pi availability at the myofilaments contributes directly to myofilament relaxation due to reversal of the myosin power stroke and especially as the myocardium transitions to relengthening in diastole.

Ciliary Ca2+ pumps regulate intraciliary Ca2+ from the action potential and may co-localize with ciliary voltage-gated Ca2+ channels.

Calcium ions (Ca2+) entering cilia through the ciliary voltage-gated calcium channels (CaV) during the action potential causes reversal of the ciliary power stroke and backward swimming in Paramecium tetraurelia. How calcium is returned to the resting level is not yet clear. Our focus is on calcium pumps as a possible mechanism. There are 23 P. tetraurelia genes for calcium pumps that are members of the family of plasma membrane Ca2+ ATPases (PMCAs). They have domains homologous to those found in mammalian PMCAs. Of the 13 pump proteins previously identified in cilia, ptPMCA2a and ptPMCA2b are most abundant in the cilia. We used RNAi to examine which PMCA might be involved in regulating intraciliary Ca2+ after the action potential. RNAi for only ptPMCA2a and ptPMCA2b causes cells to significantly prolong their backward swimming, which indicates that Ca2+ extrusion in the cilia is impaired when these PMCAs are depleted. We used immunoprecipitations (IP) to find that ptPMCA2a and ptPMCA2b are co-immunoprecipitated with the CaV channel α1 subunits that are found only in the cilia. We used iodixanol (OptiPrep) density gradients to show that ptPMCA2a and ptPMCA2b and CaV1c are found in the same density fractions. These results suggest that ptPMCA2a and ptPMCA2b are located in the proximity of ciliary CaV channels.

Quantitative real-time analysis of the efflux by the MacAB-TolC tripartite efflux pump clarifies the role of ATP hydrolysis within mechanotransmission mechanism

Tripartite efflux pumps built around ATP-binding cassette (ABC) transporters are membrane protein machineries that perform vectorial export of a large variety of drugs and virulence factors from Gram negative bacteria, using ATP-hydrolysis as energy source. Determining the number of ATP molecules consumed per transport cycle is essential to understanding the efficiency of substrate transport. Using a reconstituted pump in a membrane mimic environment, we show that MacAB-TolC from Escherichia coli couples substrate transport to ATP-hydrolysis with high efficiency. Contrary to the predictions of the currently prevailing “molecular bellows” model of MacB-operation, which assigns the power stroke to the ATP-binding by the nucleotide binding domains of the transporter, by utilizing a novel assay, we report clear synchronization of the substrate transfer with ATP-hydrolysis, suggesting that at least some of the power stroke for the substrate efflux is provided by ATP-hydrolysis. Our findings narrow down the window for energy consumption step that results in substrate transition into the TolC-channel, expanding the current understanding of the efflux cycle of the MacB-based tripartite assemblies. Based on that we propose a modified model of the MacB cycle within the context of tripartite complex assembly.

Cardiomyopathy mutations impact the actin-activated power stroke of human cardiac myosin

Cardiac muscle contraction is driven by the molecular motor myosin, which uses the energy from ATP hydrolysis to generate a power stroke when interacting with actin filaments, although it is unclear how this mechanism is impaired by mutations in myosin that can lead to heart failure. We have applied a fluorescence resonance energy transfer (FRET) strategy to investigate structural changes in the lever arm domain of human β-cardiac myosin subfragment 1 (M2β-S1). We exchanged the human ventricular regulatory light chain labeled at a single cysteine (V105C) with Alexa 488 onto M2β-S1, which served as a donor for Cy3ATP bound to the active site. We monitored the FRET signal during the actin-activated product release steps using transient kinetic measurements. We propose that the fast phase measured with our FRET probes represents the macroscopic rate constant associated with actin-activated rotation of the lever arm during the power stroke in M2β-S1. Our results demonstrated M2β-S1 has a slower actin-activated power stroke compared with fast skeletal muscle myosin and myosin V. Measurements at different temperatures comparing the rate constants of the actin-activated power stroke and phosphate release are consistent with a model in which the power stroke occurs before phosphate release and the two steps are tightly coupled. We suggest that the actin-activated power stroke is highly reversible but followed by a highly irreversible phosphate release step in the absence of load and free phosphate. We demonstrated that hypertrophic cardiomyopathy (R723G)- and dilated cardiomyopathy (F764L)-associated mutations both reduced actin activation of the power stroke in M2β-S1. We also demonstrate that both mutations alter in vitro actin gliding in the presence and absence of load. Thus, examining the structural kinetics of the power stroke in M2β-S1 has revealed critical mutation-associated defects in the myosin ATPase pathway, suggesting these measurements will be extremely important for establishing structure-based mechanisms of contractile dysfunction.

Swimming Kinematics of Cyprids of the Barnacle Balanus glandula.

Larvae of barnacles typically pass through naupliar and cyprid planktonic stages before settlement and metamorphosis. As the final larval stage, cyprids swim much faster than nauplii and in turbulent fluid environments with high shears as they seek habitat. Cyprids swim with six pairs of reciprocating thoracic appendages and use two anterior antennules during settlement. Our understanding of how thoracic appendages generate movement is limited due to short stroke intervals (∼5 ms) that impede observations of the shape and trajectory of appendages. Here, we used high-speed videography to observe both free-swimming and tethered cyprids of the intertidal acorn barnacle Balanus glandula to produce a comprehensive description of thoracic appendage swimming kinematics. Cyprids used a drag-based method of swimming: their six pairs of thoracic appendages moved through metachronal power strokes and synchronous recovery strokes similar to the thoracopod motions in calanoid copepods during escape swimming. During the power stroke, plumose setae on each appendage pair spread laterally into a high surface area and high drag paddle composed of a meshwork of fused setules. This interconnected setal array collapsed into a low surface area and low drag shape during the recovery stroke. These effective swimming appendages allowed cyprids to move upward at an average speed of 1.4 cm/s (∼25 body lengths/s) with an average beat frequency of 16 beats/s, and reach an instantaneous velocity of up to 6 cm/s. Beat frequency of the thoracic appendages was significantly associated with speed, with higher beat frequencies indicating faster swimming speed. At their average speed, cyprids moved at the intermediate Reynolds number of ∼10, in which both viscous and inertial forces affected movement. Cyprids could alter swimming direction by sweeping the posterior-most appendage pair to one side and beating the remaining thoracic appendages synchronously through the power stroke with greater motion on the outside of their turn. These results greatly enhance our understanding both of cyprid motility and how small planktonic organisms can use swimming appendages with fused setule arrays to reach high swimming speeds and affect directional changes.

Reconfigurable Curved Beams for Selectable Swimming Gaits in an Underwater Robot

Rowing is a swimming motion employed by a number of animals via tuned passive biomechanics and active gait strategies. This gait generates positive net thrust (or moment) by having a higher drag profile in the power stroke compared with the recovery stroke, which is obtained via faster actuation speed or higher effective area. In this letter, we show that using the preferential buckling of curved beams in swimming robots can, via a passive reduction of effective area in recovery stroke, be used to generate positive net thrust and moment. Additionally, these curved beams can be actively tuned to alter their behavior on demand for use in swimming applications, and can be used in an underwater robot to switch between rowing and flapping gaits. A dynamic model has been developed to model the swimming behavior of a robot using buckling joints. A design optimization has been carried out, using the Covariance Matrix Adaption Evolution Strategy (CMA-ES), to find the design and gait parameters that maximize the robot's forward swimming speed. A series of experimental gait searches have subsequently been conducted on the resulting optimal design, again using CMA-ES with the goal of finding the optimal gait pattern across a number of swimming strategies such as paddling, flapping, and undulation. By actively altering the curved beam's buckling limits, an untethered robot has been developed that maneuvers in water across each of these swimming strategies. The findings suggest that tuning the preferential buckling limits of curved beams can be an effective and potentially advantageous approach for producing directional thrust and moments.

Enhancement of Labriform Swimming Robot Performance Based on Morphological Properties of Pectoral Fins

A swimming performance underlies the biomechanical properties and functional morphology of fish fins. Recently, one of the major fields of robotics researchers' interest, is how to resolve some of the difficulties associated with the different water environments like ponds, lagoons, rivers, and deep water or even shallow, sometimes, exploration and searching in many of these environments tend to be dangerous or inaccessible to man. Thus, it is important to develop an efficient technology with direct applications as a solution to the problems appointed such as the use of swimming robots. In this research, design and realization of a swimming robot propelled by a pair of pectoral fin are implemented. The complete design of the swimming robot body is presented. Next, the effect of varying fin oscillation speed in two phases will be observed, the first phase is the power stroke one, where the fins start to push toward the backward of the body, and the recovery phase, where the fins return toward the frontal part of the body. The detailed steps of the robot design are presented and the thrust force exerted by pectoral fins has been evaluated. The suggested swimming robot is a rigid-body with an elliptical cross-sectional area, which helps in minimizing the water resistance during the thrusting process. To improve the system performance, the kinematic and dynamic model of a swimming robot has been derived based on Newton–Euler equations, while an evaluation of the total hydrodynamic forces that are exerted on the swimming robot’s body is studied via the computational fluid dynamics method. Further, a proportional integral derivative controller has been suggested for motion controlling. The proposed design has been validated theoretically via SOLIDWORKS® platform and examined experimentally by several experiments. The results of the simulation and practical experiments showed the validity of the proposed system as a labriform swimming robot.

Effects of premature contact in maxillary alveolar bone in rats: relationship between experimental analyses and a micro scale FEA computational simulation study.

The aim of the investigation was to evaluate the maxillary alveolar bone morphology, bone architecture, and bone turnover in relation to the mechanical strain distribution in rats with dental premature contact. Fifty 2-month-old male Wistar rats were used. The premature contact group (N=40) received a unilateral (right side) resin cementation on the occlusal surface of the upper first molar. The animals were distributed in 4 subgroups according to the periods of euthanasia: 7, 14, 21, and 28 days after cementation (N=10, for each period). For the control group (N=10), the teeth were kept without resin, featuring a normal occlusion. The pieces including the upper first molars, alveolar bone, and periodontal tissue were processed to histological and immunohistochemical evaluation of RANK-L and TRAP protein expression. A three-dimensional bone microarchitecture analysis was performed, where the heads of animals were scanned using microtomography and analyzed using CT-Analyser software (Bruker, Kontich, Belgium). In the computer simulation by finite element analysis, two micro-scaled three-dimensional finite element models of first molar and dentoalveolar tissues were constructed, in representation of control and premature contact groups, using Materialise MIMICS Academic Research v18 (Materialise, Leuven, Belgium). The analysis was set to simulate a maxillary molar biting during the power stroke phase. The total deformation, equivalent strain, and minimum principal strain distribution were calculated. The expression of RANK-L and TRAP presented higher positive ratio in the 7-day period compared to the control group. The three-dimensional morphometry showed decrease of bone volume in the premature contact, with significant values between the control and the 7-day and 14-day groups (P = 0.007). In FEA, the premature contact model presented a uniform compressive strain distribution in the alveolar bone crest compared to a non-uniform compressive strain distribution in the control model. The results from FEA, 3D bone microarchitecture, and histological and immunohistochemical analyses showed that a model with dental traumatic occlusion resulted in changes of alveolar bone mechanobiology and, consequently, its morphology. These results could be applied in dental treatment planning bringing biological and mechanical feedback to provide an effective mechanism to obtain physiological bone loss responses. Furthermore, this association between experimental and computational analyses will be important to figure out the alveolar bone response to mechanical stimulation in different clinical conditions.

Test of a New Low-Speed Compressed Air Engine for Energy Recovery

The paper presents a new design solution for the multi-cylinder compressed air engine, described in the PL 216801 patent. A characteristic feature of the engine is its double-piston operation with pistons working in pairs in opposition and a reciprocating movement in toroidal cylinders. The energy of compressed air was used more effectively in the described engine than in the solutions known so far. Comparing the engine built in accordance with the PL 216801 patent with the parameters of the MP165 and MP3000 engines, lower air consumption in relation to the power generated on the shaft is demonstrated. The described engine uses only one crankshaft and one straight complex shaft, which constitutes an innovative combination of pistons, while maintaining the same engine operation as in the case of two crankshafts operating with phase shift and working chamber shift. Such a solution results in a reduction in the harmful space occurring at the beginning of the power stroke to the value close to “zero”—the necessity to maintain the minimum distance between the pistons working in one cylinder when they are at their closest exists only to for the sake of collision-free operation—which is very desirable for the compressed engine operating with a shift of the working chamber. The mechanical efficiency of the engine has also been improved by guiding the pistons on the complex shaft, and the number of kinematic nodes was decreased by applying only three connecting rods supporting six pistons, which also makes it possible to improve the power and mass relation by approximately 25% in comparison with the currently known engines of similar power.

Underground locomotion in moles: kinematic and electromyographic studies of locomotion in the Japanese mole (Mogera wogura)

A series of kinematic and electromyographic (EMG) studies were conducted to characterize the neural control of underground movement in the Japanese mole, Mogera wogura. For the purposes of the present study, the locomotion of moles was classified into two modes: crawling, which comprises alternate movements of the left and right forelimbs; and burrowing, in which both forelimbs move synchronously. In crawling, moles exhibit both symmetrical and asymmetrical locomotion independent of cycle duration and speed of travel. In burrowing, the movements of fore- and hindlimbs, and of the left and right hindlimb are loosely coordinated. We divided cycles of limb movement into recovery stroke phase and power stroke phases and observed that control of cycle duration in forelimbs and hindlimbs was achieved through changes to both recovery and power stroke phases. Our results showed phasic EMG bursts in various muscles in moles, whose timing differed from that seen in terrestrial four-legged mammals such as cats and dogs. The difference was especially apparent in the m. longissimus, in which EMG bursts recorded at the level of the thoracic and lumbar vertebrae corresponded to movements of the forelimbs and hindlimbs, respectively. Thus, we conclude that moles have evolved a distinctive mechanism of neural control to perform their specialized forms of underground locomotion.

Myosin-Specific Adaptations of In vitro Fluorescence Microscopy-Based Motility Assays.

Myosin proteins bind and interact with filamentous actin (F-actin) and are found in organisms across the phylogenetic tree. Their structure and enzymatic properties are adapted for the particular function they execute in cells. Myosin 5a processively walks on F-actin to transport melanosomes and vesicles in cells. Conversely, nonmuscle myosin 2b operates as a bipolar filament containing approximately 30 molecules. It moves F-actin of opposite polarity toward the center of the filament, where the myosin molecules work asynchronously to bind actin, impart a power stroke, and dissociate before repeating the cycle. Nonmuscle myosin 2b, along with its other nonmuscle myosin 2 isoforms, has roles that includecell adhesion, cytokinesis, and tension maintenance. The mechanochemistry of myosins can be studied by performing in vitro motility assays using purified proteins. In the gliding actin filament assay, the myosins are bound to a microscope coverslip surface and translocate fluorescently labeled F-actin, which can be tracked. In the single molecule/ensemble motility assay, however, F-actin is bound to a coverslip and the movement of fluorescently labeled myosin molecules on the F-actin is observed. In this report, the purification of recombinant myosin 5a from Sf9 cells using affinity chromatography is outlined. Following this, we outline two fluorescence microscopy-based assays: the gliding actin filament assay and the inverted motility assay. From these assays, parameters such as actin translocation velocities and single molecule run lengths and velocities can be extracted using the image analysis software. These techniques can also be applied to study the movement of single filaments of the nonmuscle myosin 2 isoforms, discussed herein in the context of nonmuscle myosin 2b. This workflow represents a protocol and a set of quantitative tools that can be used to study the single molecule and ensemble dynamics of nonmuscle myosins.

A Parallel Ratchet-Stroke Mechanism Leads to an Optimum Force for Molecular Motor Function

Molecular motors convert chemical potential energy into mechanical work and perform a great number of critical biological functions. Examples include the polymerization and manipulation of nucleic acids, the generation of cellular motility and contractility, the formation and maintenance of cell shape, and the transport of materials within cells. The mechanisms underlying these molecular machines are routinely divided into two categories: Brownian ratchet and power stroke. While a ratchet uses chemical energy to bias thermally activated motion, a stroke depends on a direct coupling between chemical events and motion.

An innovative mechanism for transmitting power from the pistons in internal combustion engines

Reciprocating internal combustion engines are common and thus important engines due to the many advantages they provide. Despite significant improvements being made to these types of engines in recent decades, however, they still suffer from low efficiency due to large losses in produced energy, which occur for multiple reasons. The crank mechanism is responsible for much of the observed energy loss, however.In the current study, a new mechanism is suggested to transmit kinetic energy directly from the pistons, thus reducing energy losses associated with the crankshaft. This new mechanism eliminates the crankshaft from power transmission, limiting it to syncing piston movement. The pistons are then modified to mate with partial teething gears, which then transmit the kinetic energy directly from the pistons without this energy being negatively affected as it is by the angular position of the crank units. The testing results show that the power produced during the power stroke in the suggested engine is twice corresponding output of a classical engine.