Herein, high-performance polyvinylidene fluoride (PVDF) nanocomposite membranes were established by including a functional agent, Choline chloride (ChCl):1,5-Diphenylcarbazide (DPC) -based deep eutectic solvent (DES), into the phase inversion process, allowing the membrane structure to be tailored owing to the synergetic impact between DES components in the phase inversion process. The physicochemical properties of DES and membranes were characterized employing techniques including FTIR spectroscopy, TGA, SEM, EDX, XPS, AFM, and contact angle measurement. The membranes' performance was evaluated using milk powder solution and membrane bioreactor (MBR) effluent filtration experiments, as well as a systemic study of the impacts of varying DES loadings. The incorporation of DES into the membrane matrix enhanced the porous structure and hydrophilicity of the membranes, contributing to the enhanced water permeability (pure water flux of 215.98 ± 4.32 kg·m−2·h−1), high selectivity (removal of >99 %), and remarkable antifouling property (FRR of 98.26 %) of the resultant membranes. The long-term filtration results ample substantiated that DES could potentially be used as a modifying agent.