Evaluating antimicrobial efficacy of bovine colostrum in patients suffering from periodontal disease

Abstract

Purpose: Bovine Colostrum is the first milk, a mammary secretion produced by all cows for their babies during the first 24–48 hours after birth. Bovine Colostrum contains immunoglobulins and lactoferrin that help in bactericidal and bacteriostatic effects against microorganisms. The purpose of this study is to evaluate the efficacy of bovine colostrum at different concentrations in patients suffering from chronic periodontitis. Methodology: Plaque and calculus samples were obtained from 15 patients aging from 30 - 60 years. The collected samples were then poured on agar plates and different strains of Gram -ve bacteria were obtained. The streak plate method was adopted. For the anti-microbial susceptibility test, a solution of Bovine Colostrum Powder was prepared in Dimethyl sulfoxide (DMSO) at various concentrations (50 µg/ml, 100 µg/ml, and 200 µg/ml). Result: A minimal zone of inhibition was seen at 50 µg/ml with a mean of 3.8. Proper inhibition zones were seen in 100 µg/ml and 200 µg/ml with means of 9.9 and 12.7 respectively. One-way ANOVA showed a statistically significant difference in the values between the groups (p<0.01). Tukey HSD Post Hoc Test showed a statistically significant difference in the values between all the pairs of groups (p<0.01).