Poultry Nutrition Research Articles

Probiotics (Direct-Fed Microbials) in Poultry Nutrition and Their Effects on Nutrient Utilization, Growth and Laying Performance, and Gut Health: A Systematic Review.

Simple SummaryProbiotics are live bacteria, fungi, or yeasts that supplement the gastrointestinal flora and help to maintain a healthy digestive system, thereby promoting the growth performance and overall health of poultry. Probiotics are increasingly being included in poultry diets as an alternative to antibiotics. This systematic review provides a summary of the use of probiotics in poultry production and the potential role of probiotics in the nutrient utilization, growth and laying performance, and gut health of poultry.Probiotics are live microorganisms which, when administered in adequate amounts, confer health benefits to the host. The use of probiotics in poultry has increased steadily over the years due to higher demand for antibiotic-free poultry. The objective of this systematic review is to present and evaluate the effects of probiotics on the nutrient utilization, growth and laying performance, gut histomorphology, immunity, and gut microbiota of poultry. An electronic search was conducted using relevant keywords to include papers pertinent to the topic. Seventeen commonly used probiotic species were critically assessed for their roles in the performance and gut health of poultry under existing commercial production conditions. The results showed that probiotic supplementation could have the following effects: (1) modification of the intestinal microbiota, (2) stimulation of the immune system, (3) reduction in inflammatory reactions, (4) prevention of pathogen colonization, (5) enhancement of growth performance, (6) alteration of the ileal digestibility and total tract apparent digestibility coefficient, and (7) decrease in ammonia and urea excretion. Thus, probiotics can serve as a potential alternative to antibiotic growth promoters in poultry production. However, factors such as the intestinal health condition of birds, the probiotic inclusion level; and the incubation conditions, feedstuff, and water quality offered to birds may affect the outcome. This systematic review provides a summary of the use of probiotics in poultry production, as well as the potential role of probiotics in the nutrient utilization, growth and laying performance, and gut health of poultry.

Microbial Phytase Improves Performance and Bone Traits in Broilers Fed Diets Based on Soybean Meal and White Lupin (Lupinus albus) Meal

Abstract For several years in Europe, there has been a growing interest in the use of native sources of protein (e.g. lupin seeds) in poultry nutrition. The current study aimed to investigate the effectiveness of a microbial phytase in broiler diets with the addition of different levels of white lupin seeds. A total of 480 one-day-old male broiler chickens (Ross 308) were randomly divided into six dietary treatments (10 replications/8 birds per group). The basal diet contained SBM as the main protein source and experimental treatments were prepared with white lupin meal (WLM) at 3 levels (0, 10, and 20%) and with or without phytase inclusion. The experiment was divided into two feeding periods: from 1 to 14 days (starter) and from 15 to 35 days (grower). Diets with phytase addition were deficient in Ca and non-phytate P. All diets were fed in mash form and offered ad libitum. On day 35 excreta were collected and on day 36, ten chickens from each group were euthanized and blood, tibia, and digesta samples were collected for further analysis. The 20% addition of WLM negatively increased the content of phytic-P. The results showed that feed conversion ratio (FCR) and body weight gain (BWG) were not affected by phytase inclusion but by the WLM level alone. In addition, birds fed the diet with 20% WLM were characterized by having the lowest BWG and the highest FCR of all groups. There were no significant differences in feed intake (FI) among chickens fed control and experimental treatments. Regardless of the white lupin level addition, phytase addition improved (P<0.001) nitrogen-corrected apparent metabolizable energy (AMEN). In conclusion, the addition of phytase positively influenced the performance and availability of minerals (Ca and P) regardless of the level of WLM used. However, with regard to the use of WLM in poultry nutrition, it can be assumed that 10% addition is safe and does not affect performance.

The Processing Effects of Anthocyanins Extracted from Dragon Fruit (Hylocereus polyrhizus) Peel on Total Amount of Anthocyanins and SEM Image in Poultry Nutrition

The Processing Effects of Anthocyanins Extracted from Dragon Fruit (Hylocereus polyrhizus) Peel on Total Amount of Anthocyanins and SEM Image in Poultry Nutrition

The role of polyphenols in poultry nutrition.

In the last two decades, poultry and animal industries became increasingly interested in using plant-based feed supplements, herbs and their derivatives to retain or enhance their health and productivity. These health benefits for the host mainly attributed to the secondary plant metabolites, namely polyphenols. Polyphenols are renowned for their antioxidant, immunomodulatory, anti-mutagenic and anti-inflammatory properties. However, despite these advantages of polyphenols, they have been characterized by poor absorption in the gut and low concentration in target cells that compromise their role as effective antioxidants. The low bioavailability of polyphenols necessitates the need for further investigations to harness their full potential in poultry farms. This review is existing evidence about the bioavailability of polyphenols and their antioxidant, immunomodulatory, antimicrobial, detoxification properties and their impacts on poultry performance.

Стояновська Галина Михайлівна — кандидат біологічних наук, старший науковий співробітник (1950–2020)

Stoianovska Halyna was the candidate of biological sciences, senior researcher — qualified scientist in the physiology, biochemistry and nutrition of poultry. The main directions of research were finding substrate regulatory mechanisms of metabolic processes in the body of poultry; study patterns of physiological and biochemical processes of digestion and assimilation of nutrients in the body of poultry, and developing ways to increase the level of transformation of feed nutrients into products (meat and eggs).

Prospects of yeast based feed additives in poultry nutrition: Potential effects and applications

Yeast and its derivatives are extensively utilized as feed additives in poultry industry owing to their desirable health and growth promoting effects. Exhaustive number of studies had reported positive effects of yeast based additives on growth, meat quality, immunity, antioxidant status, and gastrointestinal functions in poultry birds. Owing to their prebiotic/probiotic properties, they also play significant role in gut development and modulation of gut microbiome by favouring beneficial microbes while reducing colonization of pathogenic microbes by competitive exclusion. They also possess effective potential for binding of dietary toxins in addition to improving digestion and utilization of nutrients. Moreover, yeast based additives have exhibited desirable effects on humoral immunity by increasing serum immunoglobulin (Ig) A levels. These additives have been also used as immune adjuvants to boost innate immune response under any pathogenic challenges in birds. Due to their diverse biological activities, yeast products are potentially capable for immune hemostasis by mediating balance between pro- and anti-inflammatory activities. These unique properties of yeast based products make them promising feed additive to promote health and productivity leading to efficient poultry production. Yeast can be supplemented in poultry diets @ 5.0–10.0 g/kg of feed. Numerous studies had reported significant improvement in body weight gain (3 to 8%) and FCR (1.6 to 12%) in broilers in response to supplementation of yeast based additives. Moreover, yeast supplementation also improved hemoglobin (Hb g/dl) levels up to 2.59 to 6.62%, total protein (>0.69%) while reducing serum cholesterol (mg/dl) up to 3.68 to 13.38%. Despite the potential properties and beneficial effects, use of yeast and its derivatives as feed additives in poultry industry is not matching its inherent potential due to many reasons. This review aims to highlight the importance and potential role of yeast and its products as natural growth promoter to replace in feed antibiotics to address the issues of antibiotic residues and microbial resistance. This article provides insights on functional role of yeast based additives in poultry diets and their importance as commercially viable alternatives of antibiotic growth promoters in poultry feed industry.

Aplicação do conceito de proteína ideal na nutrição de aves e suínos – uma breve revisão

Nutrition is responsible for most of the production costs and the formulation of balanced diets is of paramount importance to provide better productive performance and greater economic efficiency. Thus, the emergence of industrial amino acids allowed the elaboration of diets based on the ideal protein, providing an amino acid profile closer to the animals' needs, improving the use of nutrients and decreasing the excretion of dietary nitrogen in the environment. In view of the benefits presented using the ideal protein concept in the formulation of diets, this review aimed to show its application in poultry and swine nutrition

Seaweeds, Intact and Processed, as a Valuable Component of Poultry Feeds

Poultry production is an important area of the agricultural economy. Nowadays, there is an interest in novel sources of feed additives that will improve production performance and poultry health. As an easily available and renewable biomass rich in biologically active compounds, seaweeds can meet this demand. Different forms of seaweeds–seaweed powder from naturally occurring biomass, cultivated or waste biomass, extracted compounds, post-extraction residues or liquid extracts–may be used in poultry feeding. Inclusion of this unconventional material in the poultry nutrition can positively influence the poultry performance along with its health and enrich poultry products with active compounds, such as micro- and macroelements, polyunsaturated fatty acids and pigments. Seaweeds also reduce lipids and cholesterol in eggs. Moreover, due to their unique properties, they can serve as an alternative to antibiotic growth promoters. This review presents the latest developments in the use of seaweeds in poultry nutrition, as well as its limitations.

Olive Pulp and Exogenous Enzymes Feed Supplementation Effect on the Carcass and Offal in Broilers: A Preliminary Study

Nowadays, there is an increasing interest in the exploitation and valorization of agricultural food waste and by-products. At the same time, the growing demand by markets worldwide, especially in Africa and Southeast Asia, can justify the growing interest in the use of by-products for the poultry industry. Olive pulp is one of the most interesting by-products of olive tree farming (typical of the Mediterranean area), being a good source of many biologically active compounds with antioxidant, antifungal, and antibacterial properties. The presence of processed olive pulp in the diet showed to be effective in increasing the weight of specific carcass and offal traits. This work aims at studying olive pulp as a feed supplement in poultry nutrition, by focusing on the effects on broiler carcass and offal. Olive pulp (OP) is one of the by-products of olive tree farming, being the residue of olive cake after it is dried. To evaluate the effects of OP in a diet supplemented with different levels of a commercial enzyme (ENZ) blend on broiler carcass and offal traits, three hundred male broiler chicks (Ross 308 lineage; one-day-old) were divided into ten treatment groups according to a completely randomized design. The treatments diets contained: unprocessed OP (50 g/kg, 100 g/kg, 50 g/kg with ENZ, 100 g/kg with ENZ), processed OP (50 g/kg, 100 g/kg, 50 g/kg with ENZ, 100 g/kg with ENZ), and control groups (without OP, and without OP with ENZ). The OP processing increased breast percentages in broilers. Supplementation with ENZ did not change any of the studied carcass or offal trait values. The presence of OP (50 g/kg) in broiler diets increased the eviscerated carcass, leg, and neck percentage values. The presence of processed OP (50 g/kg) in the diet showed to be effective in increasing the weight of specific carcass and offal traits.

Innovación en la industria de nutrición y sanidad aviar de Argentina: estrategias de empresas intensivas en conocimiento

El objetivo de este estudio fue analizar las empresas de nutrición y sanidad aviar entre los años 2015-2018 y cómo innovaron para sostenerse en contextos globalizados. Se consideró como supuesto principal que las diferencias estaban dadas por el tipo de innovaciones desarrolladas y sus relaciones con otras firmas e instituciones. Para el trabajo fueron estudiadas -desde un enfoque sistémico sobre la innovación y en base a una encuesta- 40 empresas de capitales nacionales y multinacionales en la Argentina. Se concluyó que las empresas efectuaron diferentes tipos de innovaciones y que éstas son características diferenciadoras significativas: la innovación en servicios intensivos en conocimiento, el tipo de exportación y la cooperación tecnológica con universidades.

Fatty acids profile, cholesterol level and quality of table eggs from hens fed with the addition of linseed and soybean oil

The study objective was to assess the impact of soybean (SO) and linseed oil (LO) added to feed mixture for laying hens upon the quality characteristics of table eggs, their fatty acid (FA) profile and cholesterol level. The material consisted of eggs from hens fed with a mixture without oil and with the addition of LO or SO in a dose of 2.5%. Eggs were subjected to quality assessment. FA profile and cholesterol content in yolks were determined. The results indicate no negative changes in eggs’ quality caused by supplementation. An increase was observed in the n3 FA content in egg yolk in experimental groups, as well as all PUFA (polyunsaturated fatty acids) proportions. No negative impact of these oils on the level of cholesterol was noted. As a consequence, the analysed feed additives can be safely applied as an appropriate material in poultry nutrition to enrich eggs with PUFA.

Fifty years of sheep red blood cells to monitor humoral immunity in poultry: a scientometric evaluation

Sheep red blood cells (SRBC) are commonly employed by scientists to address humoral immune responses in poultry. While SRBC are closely related to the study of humoral immunity in poultry, the initial purpose of much research did not focus on the mechanisms involved. Here, we provide a qualitative approach and utilize scientometric techniques, including trend analyses, scientific collaborations and mapping, and word co-occurrence evaluations, to summarize the role of SRBC in the poultry studies. First, a search strategy on Web of Science (WoS) was conducted to find publications that included SRBC in the poultry studies. Publications were partitioned into 4 categories: nutrition, genetics, microbiology, and physiology. For scientometric evaluation, scientific maps and networks were produced to clarify the occurrence of SRBC in the poultry studies. Data used included 702 publications over a period of 50 y (1968-2018) that were retrieved from the WoS database. About 95% of the publications were published in English language. Indigenous, experimental, and commercial chickens, quail, and medicinal plants field/topics were the main subjects of publications. In recent years, authors have used SRBC to study humoral immune response as a secondary aim of their research, especially when poultry production/performance was studied. This was especially the case in recent decades for studies in poultry nutrition. Analysis of keywords co-occurrence showed that the phrase SRBC mostly occurred with chickens, immune response, and especially with broilers. Moreover, the “medicinal plants” are becoming important especially for research on broilers and the reduced use of antibiotics in feed. Consequently, in addition to studying the medicinal plants, finding antibiotic replacements, and/or growth performance in the birds, humoral immunity is suggested to be investigated using SRBC. Moreover, interdisciplinary studies with the cooperation of scientists from agriculture, veterinary, immunology, biochemistry and molecular biology, and toxicology will develop in the future.

Expression of selected genes encoding mechanistic pathways, nutrient and amino acid transporters in jejunum and ileum of broiler chickens fed a reduced protein diet supplemented with arginine, glutamine and glycine under stress stimulated by dexamethasone.

Reducing crude protein and supplementation with synthetic amino acids in poultry nutrition is a recent trend to avoid wastage of protein and ammonia in production systems. Stress has been shown to impair intestinal barrier and increase inflammatory response. This study was performed on intestinal tissues of broiler chickens to understand the mechanism of stress induced by a synthetic glucocorticoid, dexamethasone (DEX) and the effect of supplementation of arginine, glutamine and glycine in reduced protein diets. Intestinal tissue samples from a previous study were utilized. Male Ross 308 chickens received a basal diet for the first seven days and then fed with crude protein that was reduced to 194g/kg in grower experimental diets supplemented with glutamine, glycine and additional arginine at 10, 10 and 5g/kg respectively. Half of the 96 individual birds were injected with DEX (0.5mg/kg body weight) or saline on days 14, 16, 18 and 20 of age. mRNA expression for jejunum and ileum for amino acid transporters (y+LAT-1, Bo,+ AT, EAAT-3 and CAT-1), mechanistic genes (SGLT-1, mTOR, IAP and FABP-2) and pro-inflammatory genes (MUC-2, NF-κB, iNOS, IL-8 and IL-1β) were analysed using real-time PCR. The results showed that DEX decreased y+ LAT1 in jejunum, Bo ,+ AT and EAAT-3 in ileum. Arginine increased CAT-1 in the jejunum and ileum under DEX treatment. Through an interaction, DEX reduced IAP in jejunum of glycine and arginine supplemented group and reduced mTOR in jejunum independently. DEX reduced MUC-2 and iNOS in jejunum and increased iNOS and IL8 in the ileum. Amino acid supplementation did not appear to ameliorate these effects; however, there were some positive effects of glycine on NF-κB and arginine through increased CAT-1. Mechanistic understanding of amino acid supplementation in broiler diets warrants further research particularly when dietary protein is reduced below the level tested in the present study.

Effect of feeding different levels of lignocellulose on performance, nutrient digestibility, excreta dry matter, and intestinal microbiota in slow growing broilers

Lignocellulose is a constituent of plant cell walls and might be used as a fiber source in poultry nutrition. The current study investigated the impact of increasing dietary levels of lignocellulose on performance, nutrient digestibility, excreta DM, intestinal microbiota, and bacterial metabolites in slow growing broilers. At an age of 10 wk, 60 male broilers of an intercross line (New Hampshire × White Leghorn) were allocated to cages and fed isoenergetic and isonitrogenous diets containing 0.8% (LC1), 5% (LC2), or 10% (LC3) lignocellulose. After 23 D of feeding, broilers were killed and digesta samples of ileum and excreta analyzed for nutrient digestibility and DM. Cecal contents were analyzed for microbial composition and metabolites. Broiler performance was not affected by feeding dietary lignocellulose. LC3 fed broilers showed reduced ileal digestibility of protein compared to chickens fed LC1 (P = 0.003). Moreover, increasing levels of dietary lignocellulose reduced apparent digestibility of organic matter and gross energy (P < 0.001). Feeding of lignocellulose had no impact on the excreta DM of broilers. Increasing levels of dietary lignocellulose lowered cecal counts of Escherichia/Hafnia/Shigella (P = 0.029) and reduced the total concentration of short-chain fatty acids (P < 0.001), lactate (P < 0.05), and ammonia (P = 0.009). The molar ratio of cecal acetic acid was higher in LC3 fed broilers (P < 0.001), while the proportions of cecal propionic acid and n-butyric acid were higher in LC1 and LC2 fed chickens (P < 0.001). Correlation analyses indicated that dietary lignocellulose was negatively related to the total concentration of cecal bacterial metabolites (P < 0.001). In conclusion, the feeding of lignocellulose did not affect growth performance, but impaired nutrient digestibility of slow growing broilers. While minor changes in cecal microbial composition were detected, cecal bacterial metabolite concentrations were significantly reduced with increasing levels of dietary lignocellulose. These findings suggest that lignocellulose is not extensively degraded by bacteria residing in the large intestine of broilers.

Dietary organic cranberry pomace influences multiple blood biochemical parameters and cecal microbiota in pasture-raised broiler chickens

Abstract Cranberry processing by-products/pomace can be an excellent source of functionally bioactive molecules such as polyphenolics, complex carbohydrates, fibers, and nutritive minerals. While there are currently few applications for such processing-residues in poultry nutrition, there are many potential opportunities for the development of sustainable and value-added products. The aim of the current work was to investigate the effect(s) of four consecutive weeks of cranberry pomace (CBP) feeding on blood serum metabolic profiles and the cecal microbiota of pasture-raised broiler chickens. Six hundred day-old Cobb 500 broiler chicks were divided into three groups: one group receiving the basal diet, and two groups each receiving the basal diet supplemented with either 1 or 2% of CBP. Blood and cecal samples were collected from the birds before, during, and after the treatment over a period of 64 days for biochemical and 16S rRNA gene sequencing analysis, respectively. The detailed analysis of CBP (and formulated final feed) indicated its content in essential amino- and fatty acids in addition to its richness in dietary fibers. Incorporating the pomace in feed induced a dichotomous response in broilers, with short-term improvements in blood-serum iron and cholesterol levels coupled with long-term modulation of cecal microbiota characterized by an increase in beneficial bacterial taxa (including Bifidobacterium, unclassified_Rikenellaceae, and Faecalibacterium) while decreasing the presence of undesirable ones (unclassified_Synergistaceae and Desulfovibrio, and unclassified_Fusobacteriaceae). Overall, the outcome of this study suggests the possibility of using organic CBP as a feed supplement with potential ability to positively influence blood metabolites and gut microbial community composition in pasture-raised broiler chickens.

Dried fruit pomace inclusion in poultry diet: growth performance, intestinal morphology and physiology

BackgroundFruit pomaces are by-products rich in polyphenol compounds and dietary fiber. They seem to play an important role in regulating the gut microbiota, morphology and physiology. The aim of this study was to assess whether apple (A), blackurrant (B) or strawberry (S) pomaces could be suitable ingredients in broiler diets and their effect on gut health. A total of 480 male broilers were randomly allotted to 8 dietary treatments with lower (3%-L) or higher (6%-H) dietary fiber content: two control groups (CL/CH), two A diets (AL/AH), two B diets (BL/BH), two S diets (SL/SH). Diet and fruit pomaces were chemically analyzed to assess polyphenol concentration and fibre fraction content. After the evaluation of growth performance, 6 birds/group were slaughtered at 35 days of age. Morphometric and histopathological investigations were performed on duodenum, jejunum and ileum. Excreta were collected to perform microbiota evaluation by 16S DNA sequencing. Weight, viscosity, enzymatic activity, short chain fatty acid (SCFAs) and ammonia concentration were determined in ileum and/or ceca content.ResultsA pomace and A diets showed the lowest polyphenol content and the highest content of soluble fibre fraction. No significant differences were observed for growth performance, gut morphometry and histopathology (P > 0.05). Dietary fruit pomace inclusion increased the weight of ileum and ceca and the ileum digesta viscosity (P < 0.05). In the ileum, A and S groups showed lower bacterial α-glucosidase activity than C groups. Moreover, small intestine SCFAs concentration was higher in fruit pomaces diets (P < 0.05). In ceca, B and S groups showed lower ammonia concentration and higher SCFAs than C. Dietary treatments also influenced the activity of α-glucosidase, α-galactosidase, β-galactosidase β-glucuronidase and xylase. Regarding microbiota, at phylum level, Firmicutes were differentially abundant across treatment (maximum for C and minimum in S, FDR > 0.05). At genus level, an increase of Weissella in AH and Erwinia in S/B diets, as well as a decrease of Lactobacillus in all fruit pomace groups were recorded (P < 0.05).ConclusionsFruit pomaces could be suitable ingredients in poultry nutrition even if further studies are needed to better understand which doses is more recommended to avoid negative effects on gut microbiota.

Influence of Oyster Mushroom Waste on Growth Performance, Immunity and Intestinal Morphology Compared With Antibiotics in Broiler Chickens.

Oyster mushroom waste (OMW) is a by-product of the agriculture industry with valuable antimicrobial, antioxidant, antifungal, and prebiotic properties. This by-product might be a useful alternative to antibiotic growth stimulators in poultry nutrition. The purpose of this research was to test the impact of OMW on the immune responses and on the morphology of intestine of broiler chickens. Four dietary therapies with five replicas of 15 birds in each, totalling 300 day- Ross 308 broiler chickens, were utilized in this study. Control chickens were fed a mixed diet that included a maize-soybean meal complemented by 1 and 2% OMW in addition to the basal diet. Furthermore, Enramycin (125 g/kg) was added to the control diet as an antibiotic. Throughout this experiment, performance was studied as well as the immune response to the Newcastle Disease Virus (NDV) and intestinal morphological traits. A substantial surge was noted in body weight gain (BWG) and feed intake (FI) of chickens after the addition of 1% OMW (p ≤ 0.05). In contrast, feed supplementation with 2% OMW, compared with the control diet, produced no noteworthy increase in BWG or the feed conversion rate (FCR). Antibiotic addition, on the other hand, increased serum cholesterol (p ≤ 0.05). After 42 days, neither OMW nor antibiotic addition affected organ mass. In contrast, antibiotic addition reduced the small intestine percentage, crypt depth and villus height (p ≤ 0.05). The Newcastle disease vaccine (NDV) antibody titer improved after feed supplementation with 1% OMW comparing with the control and antibiotic diet group. Furthermore, OMW supplementation decreased the heterophil-to-lymphocyte H/L ratio (p ≤ 0.05). The use of OMW led to a reduction in the malondialdehyde (MDA) content of the breast and liver and an increase in glutathione peroxidase. It helped to reduce glutathione, glutathione reductase, and glutathione S-transferase. In conclusion, the impact of OMW were dose-dependent, and the use of 1% OMW in broiler diets enhanced their growth and immunity. Nonetheless, supplementation with 2% OMW produced conflicting results.

Effect of betaine, a methyl group donor, on broiler chicken growth performance, breast muscle quality characteristics, oxidative status and amino acid content

This study was aimed to investigate the effect of feed supplemented with betaine on broiler chickens’ growth and slaughter performance, breast muscle histomorphometric and physicochemical properties, oxidative status and amino acid content. A total of 1000 1-day-old Ross 308 broiler chickens were divided into four treatments. Control group chickens were fed with standard compound diet (SCD), the chickens from experimental groups B1, B2 and B3 receiving SCD supplemented with 1 g/kg (B1), 2 g/kg (B2) and 3 g/kg (B3) betaine anhydrous, respectively. Each treatment had five replicate pens. Feeding test results showed that betaine reduced broilers’ mortality but increased feed conversion ratio (p < .05). Forty broiler chickens (5 weeks old) were slaughtered and slaughter performance showed that 2 g/kg betaine inclusion improved breast muscle percentage and yield (p < .05). Betaine dosage of 1 g/kg into feed increased breast muscle fibre areas (p < .05). Betaine affected some physicochemical properties: higher a* and the highest drip loss in B2; the highest cooking losses in B1, B2; the highest shear force and fat content in SCD; the highest amounts of ashes in B1, B2 (p < .05). Lower malondialdehyde levels were observed in all betaine-treated groups (p < .05), except B1 fresh meat samples. The highest total amino acid content and a greater amount of essential amino acids were obtained in SCD breast muscles (p < .05), except equally highest amounts of methionine were found in both SCD and B3 samples (p < .05). However, according to our study results, betaine, as a methyl group donor, in broiler chicken diets cannot replace methionine as an essential amino acid. Highlights Betaine is known as functional nutrient in poultry nutrition, which can fulfil the function of a methyl donor. Betaine in animal feed is saving feed costs by replacing choline chloride and methionine.

Performance of Slow-Growing Male Muscovy Ducks Exposed to Different Dietary Levels of Quebracho Tannin.

Simple SummaryDifferent inclusion levels of Quebracho tannin (QT) in the diet of growing male Muscovy ducks of a slow-growing type were explored under free-range conditions. As a result of the dietary treatments tested in this trial, the growth performance or the total blood proteins were not affected. By contrast, dietary QT did not lead birds to produce less moist excreta, as observed in other bird species, as a desirable aspect for intensively raised poultry. A marked improvement in carcass yield was observed as a desirable economic trait in the extensive slow-type duck farming system. The study of the nutritional effects of tannins is complex due to the large chemical diversity; consequently, in poultry nutrition the biological responses may vary greatly. The aim of the present study was to evaluate the effect of different levels of dietary Quebracho tannins (QT) on growth and production performance in slow-growing type Muscovy ducks. For this purpose, a 42-d trial was carried out on 126 male ducks (42-d old at start), fed on three levels of dietary QT inclusion in the diet (0% as control diet, vs. 1.5% vs. 2.5% on an as fed basis). Birds were reared under free-range conditions. A linear increase in feed intake as a function of QT inclusion in the diet was observed (p < 0.05). No difference as to final body weight, overall average daily weight gain (ADG) and total feed conversion ratio (FCR) in relation to dietary treatments was observed. Carcass yields were positively improved in QT birds (p < 0.05). No adverse responses were recorded in total blood protein and liver weight. Dietary QT might be safely used up by to 2.5% in 42- to 84-d aged male Muscovy ducks.

Influence of Dietary Supplementation of Salix alba Bark on Performance, Oxidative Stress Parameters in Liver and Gut Microflora of Broilers.

Simple SummaryNowadays, due to genetic evolution, poultry is more susceptible to stressful factors (nutritional, environmental, etc.), which causes imbalances in the redox status, namely oxidative stress. Among the consequences of oxidative stress in poultry are impaired performances, gut microflora imbalance, as well as the deterioration of the overall health status. Consumer pressure about the quality and safety of animal products has prompted the need for use, among many dietary factors, of natural antioxidants. Salix alba bark represents a source of bioactive compounds, including polyphenols and flavonoids, with an important role in poultry nutrition. This study aims to describe the effects of dietary Salix alba bark powder on the performance of oxidative stress parameters in liver and gut microflora of broilers. As conclusion, the paper proposes that dietary 0.05% Salix alba bark powder could be an effective solution to impede the oxidative stress in broiler liver and to improve gut microflora. The paper manifests noteworthy concern in poultry nutrition, whereas many diseases have been related to oxidative stress.The paper aimed to analyse the effect of dietary Salix alba L. bark powder (SAB) on broiler performance in terms of oxidative stress parameters in liver and gut microflora. One hundred and eighty Cobb 500 broiler chicks (14 days) were allotted to three homogeneous treatments (SAB 0%; SAB 0.025%; SAB 0.05%). The broilers were housed in an environmentally controlled space (10 replicates, six broilers/replicate). Compared to dietary control treatment (SAB 0%), the other treatments included 0.025% SAB (SAB 0.025%) and 0.05% SAB (SAB 0.05%). The results showed that SAB powder used in broiler diet had a high total phenolic content. Regarding the performance results, significant differences between experimental and control treatments were recorded only for average daily feed intake (35–42 days). The broilers fed with SAB powder had a significantly lower hepatic level of malondialdehide and glutathione, a higher total antioxidant capacity than those fed control treatment, and demonstrated a positive effect on the development of non-pathogenic bacteria (lactobacilli) but a decrease in the population of pathogenic ones (E. coli, staphylococci). Our findings suggested that dietary 0.05% SAB powder could be an effective solution to impede the oxidative stress in broiler liver and to improve gut microflora.