Poultry Meat Meal Research Articles

Apparent digestibility of protein and essential aminoacids from commonly used feed ingredients in Brazil for juvenile shrimp Litopenaeus vannamei

This study determined the apparent digestibility for crude protein (ACPD) and essential aminoacids (AEAAD) of ingredients for Litopenaeus vannamei fed diets containing soy protein concentrate (SPC), corn gluten meal (CGM), poultry byproduct meal (PBM), meat and bone meal (MBM), hydrolyzed feather meal (HFM), spray-dried blood meal (DBM), tilapia byproduct meal (TBM), Brazilian marine fish meal (BFM), salmon byproduct meal (SLM), and krill meal (KRM). Digestibility was estimated using chromic oxide as a marker in a reference (REF) mixture. Shrimp [...]

Future feed resources in sustainable salmonid production: A review

AbstractAquaculture is one of the most resource‐efficient and sustainable ways to produce animal protein. The Food and Agriculture Organization predicts that cultivated aquatic species will provide around 53% of the world's seafood supply by 2030. Further growth of intensive farmed aquatic species may be limited by a shortage of feed resources. The aquaculture sector therefore needs to intensify its search for alternative ingredients based on renewable natural resources. A significant increase in production will require an accelerated transition in technology and production systems, better use of natural available resources, development of high‐quality alternative feed resources and exploitation of available space. The present review discusses the urgent need to identify appropriate alternative ingredients for a sustainable future salmonid production. We describe and evaluate the most promising marine ingredients, including low‐trophic species (mesopelagic fish, zooplankton, polychaetes, macroalgae and crustaceans), novel microbial ingredients (bacteria, yeast and microalgae), insects (black soldier fly, yellow meal worm and crickets), animal by‐products (poultry meal, meat and bone meal, blood meal and hydrolysed feather meal) and by‐products from other commercial productions (trimmings and blood). Furthermore, we discuss the available volumes and need for new processing technologies and refining methods to ensure commercial production of nutritionally healthy ingredients. The essential production steps and considerations for future development of sustainable and safe seafood production are also discussed.

ENERGY AND PROTEIN INGREDIENTS FOR USE IN PIAPARA (<i>Megaleporinus obtusidens</i>) DIETS: NUTRITIVE VALUE AND INTESTINAL MORPHOMETRY

The apparent digestibility coefficients for crude protein (ADCCP), dry matter (ADCDM), and gross energy (ADCGE) of ingredients were determined for piapara (Megaleporinus obtusidens). Test diets were formulated to contain 69.5% of reference diet, 0.5% of chromium oxide, and 30% of test ingredients. The protein ingredients evaluated were tilapia processing residue meal (TPRM), feather and poultry blood meal (FPBM), poultry by-product meal (PBM), meat and bone meal (MBM), cottonseed meal (CM), corn gluten meal (CGM), and soybean meal (SM); the energy ingredients tested were corn (C), corn germ meal (CGRM), rice meal (RM), wheat bran (WB), and sorghum (S). Groups of 30 piaparas were fed twice daily during five days with test diets. Intestinal morphometry of fish were also evaluated. Digestibility coefficients of protein and energy ingredients were highest for soybean meal (ADCDM = 85.8%; ADCCP = 95.2%; and ADCGE = 87.2%) and corn (ADCDM = 94.5%; ADCCP = 76.2%; and ADCGE = 89.3%), respectively. Of the energy test ingredients analyzed, corn had the highest digestibility coefficients and induced beneficial changes on intestinal morphology compared to sorghum and corn germ meal. All protein ingredients showed potential for use in piapara diets, except meat and bone meal .

Free Choice Feeding on the Quality of Quail Eggs (Coturnix coturnix-japonica)

Quail is one of the potential sources of animal protein from poultry as egg producers. An egg contains complete nutrients, namely protein, fat, vitamins, and minerals. As food, eggs must be maintained both physically and chemically to be safe for consumption. This study aimeds to examine the effect of selective feeding on the quality of quail eggs. This research was carried out at Percut Sei Tuan Subdistrict, Deli Serdang Regency, North Sumatra, which had been implemented from August to November in 2018. The study used a completely randomized design with 4 treatments and 5 plots. The study used egg samples from quail aged five (5) months. The treatment consists of Commercial feed (T0), 2 energy source feed (rice bran, corn) with 2 (T1), 3 (T2), 4 (T3) protein source feeds (fish meal, soybean meal, coconut meal, Poultry Meat Meal). The results showed that the treatment gave a significant effect (P<0.05) on egg weight, albumen, and eggshell weight, commercial feed showed eggs weight higher than free choice feeding treatments, while among free choice feeding treatment did not show significant effect. The free choice feeding treatment has a significant effect on the yolk weight, where T2 has the lowest yield. Percentage of yolk, albumen, quail eggshell and egg index, egg yolk index, albumen index, haugh unit and quail yolk color did not show significantly different (P>0.05) among treatment. It can be concluded that the commercial feed showed higher egg weight but had no significant effect (P>0.05) on the proportion of eggs and the interior quality of quail eggs, while among free choicee feeding treatment has no effect.

Effects of different dietary protein sources on the immunological and physiological responses of marron, Cherax cainii (Austin and Ryan, 2002) and its susceptibility to high temperature exposure

A two phased feeding trial was conducted to evaluate the effects of alternative protein sources on the immunophysiological responses of marron. During the phase I, marron were fed with five alternative protein supplemented diets for 90 days, while in phase II, the same marron were exposed to elevated temperature (30 °C) and their immunophysiological responses were investigated post exposure. Five isoproteic (crude protein 30%) and isoenergetic diets were prepared by containing fishmeal, poultry by-product meal, feather meal, lupin meal, and meat and bone meal as the main protein source. A hundred and fifty juvenile marron (Cherax cainii) of the average weight 9.09 ± 0.21 g were randomly distributed into 15 tanks (three replicates per feeding treatments). In the Phase I, general immune response parameters, such as, total haemocyte count (THC), proportion of hyaline cells, neutral red retention time (NRRT), phagocytic rate (PR), heamolymph bacteraemia, and condition indices of marron were investigated. The highest (P < 0.05) THC among dietary protein sources was obtained in marron fed with PbM at the end of experiment. Marron fed with FeM protein sources resulted in the highest survival rate followed by PbM fed group. Longer microvilli length (3.83 ± 0.18 μm) was demonstrated in marron fed with PbM diet. Diets containing FM and PbM protein sources revealed significantly (P < 0.05) lower number of microvilli/group than diets containing FeM and LM. The results demonstrated that different dietary protein sources in the marron diets did not detect significant (P > 0.05) change of the condition indices throughout the experiment period, however highest Hiw and Hid was recorded in marron fed with PBM at day 45. The PR of marron fed dietary protein from PbM did not change significantly after temperature exposure. Increased NRRT, PR and haemolymph bacteraemia was observed with dietary feeding of FM at the end of the trial. However, results revealed that PbM could be an alternative protein source for culture of marron as reflected in terms of increased THC, longer microvillus length and improved susceptibility to high temperature exposure. Overall, result could serve as useful baseline data in developing cost effective potential diets for marron aquaculture.

Physical and Sensory Quality of Broiler Meat as Influenced by Dietary Supplementation of Turmeric (Curcuma longa), Garlic (Allium sativum) and in Combinations as a Feed Additive

This research was conducted to evaluate the effect of turmeric water extract, garlic and combinations of turmeric and garlic as feed additives in broiler diets on the physical and sensory quality of broiler meat. During the study, the chicken were given 5 feeding treatments, i.e. R0 (basal ration without phytobiotic and antibiotics) , R1 (basal diet + 0.015% zinc bacitracin + 2.485% filler), R2 (basal ration + 2.50% TE), R4 (basal ration + 2.00% GE + 0.50% filler), and R5 (basal ration + 2.50% TGE). The base diet was composed of: yellow corn, meat and bone meal, poultry meat meal, soybean meal, oil, mineral mix, calcium carbonate, dicalcium phosphate, salt, L-lysine-HCl, and DL-methionine. Variables observed were physical quality (pH, water holding capacity, cooking lose, and tenderness) and sensory quality. Data regarding physical quality were statistically analyzed by one-way ANOVA followed by the orthogonal contrast for significant results, and data of sensory quality by non-parametric methods such as the Hedonic Kruskal-Wallis test. The results showed that the physical quality of meat had no effect (P&gt;0.05), except the significant water holding capacity (P&lt;0.05) which was lower with phytobiotic extract supplementation. All sensory test parameters in fresh and cooked meat had no effect (P&gt;0.05) following phytobiotic extract supplementation, except for the color and acceptability (P &lt;0.05) in fresh broiler meat. The conclusion of the research was that 2.5% TE, 2.0% GE and 2.5% TGE is capable of acting as a feed additive to increase the physical and sensory quality of broiler meat.

Validation of processed animal proteins (mono-PAPS) in experimental diets for juvenile gilthead sea bream (Sparus aurataL.) as primary fish meal replacers within a European perspective

Experimental diets were formulated to evaluate a “pure” poultry meat meal (PMM) source in diets formulated for juvenile gilthead sea bream (Sparus aurata L.). The digestible protein contribution of fish meal in a control diet was substituted by 25%, 50% and 75% of a processed poultry meat meal (PMM) on a digestible crude protein (DCP) basis and by 5% and 10% for an enzyme‐treated feather meal (EFM) and also a spray‐dried haemaglobin meal (SDHM), respectively. In a consecutive trial, diets were designed to assess the value of a “pure” (defatted) poultry protein substituting the fish meal (FM) protein content. Experimental diets included: a control diet, two test diets where 75% of FM was replaced by a full‐fat PMM (PMM75) or a defatted grade of PMM (dPMM75) and two test diets where 50% of FM was substituted for defatted PMM (dPMM50) or a 50:50 blend of soya bean meal and defatted PMM (SBM/dPMM) to produce a composite product. This soya bean/dPMM blend was tested to enhance the nutritional value of this key plant ingredient commonly employed in sea bream diets that can be deficient in specific amino acids and minerals. In the first trial, gilthead sea bream grew effectively on diets containing up to the 75% replacement of FM attaining a mean weight of 63.6 g compared to 67.8 g for the FM control fed group. For the consecutive trial, the fishmeal‐based control diet yielded the highest SGR followed by dPMM50 and SBM/dPMM blend inclusion but was not significant. Carcass FA profiles of gilthead sea bream conformed to the expected changes in relation to the dietary FA patterns, with the 18:1n‐9 representative of the poultry lipid signature becoming more apparent with PMM inclusion. The ratio of n‐3/n‐6 fatty acids was greatly affected in sea bream fed the full‐fat PMM at 75% inclusion due to fish oil exclusion. Defatted dPMM, however, allowed more of the fish oil to be used in the diet and reducing this latter effect in sea bream carcass, hence restoring the higher total omega‐3 HUFA fatty acids namely EPA and DHA and n‐3/n‐6 ratio. It is concluded that poultry meat meal can be modestly incorporated into formulated diets for sea bream and can be used in conjunction with soya bean meal without any fundamental changes in performance and feed efficiency.

Apparent Digestibility of Minerals from Several Ingredients for Tambaqui, Colossoma macropomum, Juveniles

AbstractBased on the importance of tambaqui, Colossoma macropomum, for Latin American aquaculture and the digestibility of minerals for the production of sustainable aquafeeds, we designed a series of trials to determine the apparent digestibility coefficients (ADCs) of phosphorus, calcium, magnesium, manganese, and zinc from six protein sources: soybean meal (SBM), corn gluten meal 60 (CGM60), cottonseed meal 40, fishmeal (FM), poultry byproduct meal (PBM), and meat and bone meal (MBM); four cereal grain products: corn, wheat middlings, broken rice (BR), and rice bran (RB); and dicalcium phosphate (DCP). Considerable variation in the ADCs of minerals was observed among the feed ingredients. In general, CGM60, PBM, and RB tended to show the highest ADC values, while SBM, MBM, DCP, and BR showed the lowest ADC values. ADC‐P was markedly lower in animal protein sources and the phosphate source (DCP), while high P digestibility was observed for plant protein sources and cereal grain products. Interestingly, tambaqui seems to be able to digest and absorb minerals from plant ingredients more efficiently than animal proteins and DCP. In light of the correlation between nutrient levels and the ADCs of minerals, physiological mechanisms that regulate mineral uptake, and the nutritional ecology of the species, we hypothesize that tambaqui are able to digest minerals from plant proteins with high efficiency.

Element speciation in UK biomass power plant residues based on composition, mineralogy, microstructure and leaching

Biomass ash management is an escalating issue in many countries because of increasing numbers of biomass power plants. Comprehensive characterization of biomass ashes with emphasis on element speciation, and solubility of nutrients (e.g., K and P) and pollutants is essential for potential utilization of these residues for soil nutrition. All the UK biomass ashes investigated, whether from combustion of poultry litter, meat and bone meal, and straw, were alkaline and contained high concentrations of P, K, and Ca. The biomass air pollution control (APC) residues were enriched in K, Cl, S and Zn, and contained less lithophile elements, such as Al, Ca, P, Mg, Si, Ti, and Ba, compared to the bottom ashes. P appeared in: 1) bottom ashes as apatite and other phosphates (potassium hydrogen phosphate and potassium iron phosphate in the bottom ashes from combustion of poultry litter); 2) APC residues from combustion of poultry litter as potassium sodium calcium phosphate. K is present mainly in sylvite, arcanite, and some phosphates. Na, K, Cl, and S were easily leached by water from the biomass APC residues. However, water leaching of P, Ca, and Mg was very low, with leaching of P possibly controlled by hydroxyapatite. Aqueous Zn, Cu and Pb appear to prevail in the form of neutral and anionic hydroxide complexes, which are toxic and easily accessible chemical forms for live organisms. Application of the poultry litter bottom ashes as a PK fertiliser in agriculture is appropriate. However, direct application of APC residues to agricultural fields is not appropriate but recovery of K and P from that material should be considered.

The Effect of Soursop (Announa Muricata L.) Leaves Powder on Diameter of Muscle Fiber, Lipid Cell, Body Weight Gain and Carcass Percentage of Tegal Duck

The present study investigated the supplementation of soursop leaves powder (Annona muricata L.) on body weight gain and carcass percentage of male Tegal duck. Research was conducted from 29 November 2015 to 3 January 2016 in duck cage in Sokaraja Kulon, Purwokerto. One hundred male Tegal duck were fed basal feed consisted of 30% corn, 7% soy bean meal, 6,1% vegetable oil, 17% poultry meat meal, 38,2% ricebran, 0,1% L-lysin HCL, 0,3% DL-methionin, 0,2% topmix, 0,1% NaCl, and 1% CaCO3. Experimental research used completely randomized design with treatments composed of basal feed plus 0, 5, 10, and 15% soursop leaves meal, each with 5 replicates. The observed variables were diameter of muscle fiber, lipid cell, body weight gain, and carcass percentage. The obtained data were subject to analysis of variance followed by orthogonal polynomial test. Result showed that treatments affected non significantly (P&gt;0.05) to the diameter of chest muscle fiber, carcass percentage and carcass but significantly affected (P&lt;0,05) body weight gain with equation Y = 427,74 - 67,10 X + 2,27 X2.. Conclusively, supplementation of soursop leaves meal (Annona muricata L.) in feed has not been able to increase the muscle fiber diameter of intermuscular lipid cell, carcass percentage and carcass parts. Excessive supplement even lowers the body weight gain of male Tegal duck.

Technical note: Characterization of lipid constitution in Fourier transform infrared spectra and spectroscopic discrimination of animal-derived feedstuffs from different species.

The objective of the current work was to assess the capability of Fourier transform infrared (FT-IR) spectroscopy in combination with chemometric methods to discriminate animal-derived feedstuffs from different origins based on the lipid characteristics. A total of 82 lipid samples extracted from animal-derived feedstuffs, comprising porcine, poultry, bovine, ovine, and fish samples, were investigated by gas chromatography and FT-IR. The relationship between the lipid constitutions and the responding FT-IR spectral characteristics were explored. Results indicated that high correlations ( > 0.900) were found between the contents of MUFA and PUFA and FT-IR spectral data. In addition, the peak intensity at about 1,116 and 1,098 cm-1 showed a significant difference ( < 0.05) between ruminant and nonruminant animals; the change of peak ratio (1,116:1,098) was proved consistent with the degree of unsaturation of lipid from different animal species. Successful discrimination was further achieved among porcine, poultry, bovine, and ovine meat and bone meal (MBM) and fishmeal based on lipid characteristics by applying the FT-IR spectra coupled with chemometrics, for which the values of sensitivity and specificity were close to 1 and classification error were almost equal to 0.

Amino acid digestibility of animal protein ingredients for bullfrogs in different phases of post-metamorphic development

The development of diets that economically and ecologically meet the requirements of bullfrogs (Lithobates catesbeianus) is important for frog farming. Therefore, the objective of this study was to determine the apparent digestibility coefficients for essential amino acids of eight animal feed ingredients (poultry byproduct meal, meat and bone meal, salmon byproduct meal, tilapia byproduct meal, spray-dried blood meal, powered red blood cells, feather meal and sardine meal) used in the diets of bullfrogs. Additionally, the limiting digestible amino acids were determined for each ingredient. Bullfrogs in different phases of post-metamorphic development were used: 810 animals in the early phase (mean weight: 40 ± 10 g), 405 animals in the growing phase (95 ± 15 g) and 243 animals in the finishing phase (175 ± 25 g). Poultry byproduct meal, salmon byproduct meal and blood meal exhibited the largest number of high apparent digestibility coefficients for essential amino acids (>70%) in the early, growing and finishing phases, respectively. The animal protein ingredients were well digested by the bullfrogs and could be used in practical diets for this species, respecting the limits of inclusion and digestibility values for each developmental phase.

The Apparent Digestibility Coefficients of 13 Selected Animal Feedstuff for Cobia,Rachycentron canadum

AbstractThe apparent digestibility coefficients (ADCs) of crude protein, lipid, phosphorous, and amino acids, as well as energy, of 13 animal feedstuffs were determined for cobia,Rachycentron canadum(initial weight 130.0 g). The feedstuffs tested included five types of fishmeal, two of blood meal, one of poultry meat meal, two of meat‐and‐bone meal, two of feather meal, and one type of shrimp meal. A reference diet was formulated and the test diets contained 70% of the reference diet and 30% of each feedstuff. TheADCsof crude protein, lipid, energy, phosphorous, and amino acids of the test ingredients ranged from 68.97 to 92.5%, 69.3 to 95.7%, 77.5 to 98.3%, 58.2 to 80.1%, and 72.5 to 97.0%, respectively. TheADCsof crude protein of white fishmeal, steam‐dried brown fishmeal, imported flame‐dried fishmeal, spray‐dried blood cells, poultry byproduct meal, meat‐bone meal, and meat meal were significantly higher than those of dried blood meal, fermented feather meal, and hydrolyzed feather meal (P &lt; 0.05). TheADCsof lipid of dried blood meal and hydrolyzed feather meal were significantly lower than those of other feedstuffs (P &lt; 0.05). TheADCsof energy of fermented feather meal and hydrolyzed feather meal were significantly lower than those of other feedstuffs (P &lt; 0.05) and theADCsof phosphorus of white fishmeal, steam‐dried brown fishmeal, and imported flame‐dried fishmeal were significantly higher than those of the other ingredients (P &lt; 0.05).

Total leukosit dan diferensial leukosit darah ayam broiler akibat penggunaan tepung onggok fermentasi rhizopus oryzae pada ransum

The aim of the present study was to determine the effect of cassava pulp fermented with Rhizopus oryzae on the total leucocyte and differential leucocyte and its influence on the health of broiler chickens. The study was conducted in July-September 2015. The material used was 275 DOC of broiler with average of initial weight 55 g. Ration ingredient used were corn, rice bran, soybean meal, fish meal, poultry meat meal and fermented cassava pulp. The experiment used a completely randomized design (CRD) comprised of 5 treatments and 5 replications. Treatment ration consist of T0 (control ration), T1 (8% cassava pulp without fermentation), T2 (8% cassava pulp fermented with Rhizopus oryzae ), T3 (16% cassava pulp fermented with Rhizopus oryzae ) and T4 (24% cassava pulp fermented with Rhizopus oryzae ). The result of the study showed that there was no influence among treatments (P>0,05) of the use of cassava pulp fermented with Rhizopus oryzae in ration to total leucocyte and differential leucocycte. It is concluded that cassava pulp fermented with Rhizopus oryzae in ration is potential to substitute the use of corn in ration without compromising the health and amount of total leucocyte and differential leucocyte. Keywords : Rhizopus oryzae, leuycocyte, differential leucocyte, broiler

Determination of Ileal Amino Acid Digestibilities of Some By-products for Chickens

The aim of this study was to determine the standardized ileal digestibility of amino acids in six by-product ingredients for broiler, i.e., copra meal, rapeseed meal, feather meal, hydrolyzed poultry meal, meat and bone meal, and shrimp head meal. Luong Phuong chickens at 35 days of age were used in the trials. Dietary protein in all assay diets was supplied solely by the test ingredient. In the case of protein-rich ingredients, dextrose was added to assay diets to obtain 20% of crude protein. Standardized ileal amino acid digestibilities were calculated by correcting the apparent ileal digestibility coefficients by basal endogenous amino acid losses. Results of study showed that the variation in ileal digestibility coefficients of amino acids was low in rapeseed meal and high in copra meal. Among animal protein meals, the lowest variation of digestibility among amino acids was observed in shrimp head meal. The high standardized ileal digestibilities of Arg, His, Leu + Ile, Thr, Trp, Val, and Phe were observed in hydrolyzed poultry meal and shrimp head meal. Meanwhile, the standardized ileal digestibility values of Lys, Thr, and Trp in feather meal were very low. Meat and bone meal and feather meal was the two least digestible amino acid ingredients.

A new approach for species discrimination of different processed animal proteins based on fat characteristics

A new method for discriminating processed animal proteins (PAP) from different species based on their fat characteristics was established for the first time in this study. A total of 53 reliable PAP samples containing fish meal (FM), porcine, poultry, bovine, and ovine meat and bone meal (MBM) were collected, and 37 distinct fatty acids were statistically analyzed via gas chromatography. Principal component analysis and the partial least squares discriminant analysis algorithm were performed on the dataset. The results revealed that PAPs from different species could be clearly classified based on the presence or absence of fatty acid compounds with high sensitivity and specificity (&gt;0.95), except for the discrimination of bovine MBM in the mixed dataset. It was possible to distinguish FM samples in the mixed dataset, ruminant MBM from non‐ruminant MBM and FM, and porcine MBM from poultry MBM samples based on the concentrations of 34 fatty acid compounds. Further discrimination of PAP samples from different species using fewer selected fatty acids was comparable to or better than discrimination based on the presence or absence and concentration of 34 fatty acid compounds.Practical applications: A new method for discriminating between processed animal proteins (PAP) from different species based on their fat characteristics was established for the first time in this study. Results suggested that the analytical methodology established in this study has great potential for species‐specific identification of PAP feed with respect to the global “mad cow” crisis.PCA visualisation based on the presence or absence of fatty acid, MBM, meat and bone meal, FM, fish meal.

Feasibility of partial replacement of fishmeal with proteins from different sources in diets of Korean rockfish (Sebastes schlegeli)

An 8-week feeding experiment was conducted in an indoor recirculation seawater system to investigate the effects of partial replacement of dietary fishmeal with proteins from five sources on the growth performance and feed utilization of Sebastes schlegeli. Six isonitrogenous and isoenergetic diets were formulated using fishmeal (FM, the control) as sole protein source, or proteins from five sources including poultry by-product meal (PBM), meat and bone meal (MBM), soybean meal (SBM), cottonseed meal (CSM) and canola meal (CNM). Fifteen percent of the crude protein provided by fish meal was replaced, respectively. The results showed that the differences in specific growth rate (SGR) and survival rate (SR) among fish fed PBM, MBM, SBM, CSM and whole FM diets were not significant. However, SGR and SR of fish fed CNM diet was significantly lower than that of other treatments. Feeding rate, feed conversion, nutrient retention showed similar patterns to that of growth. Fish fed CSM and CNM showed significantly lower apparent digestibility coefficient (ADC) of dry matter and gross energy than those fed others while fish fed CNM showed lower ADC of crude protein than those fed others (P < 0.05). These results suggested that it was feasible to substitute 15% dietary protein provided by fishmeal with PBM, MBM, SBM and CSM, respectively, but not with CNM as the replacement with CNM reduced fish growth and feed utilization.

Protein quality of insects as potential ingredients for dog and cat foods.

Insects have been proposed as a high-quality, efficient and sustainable dietary protein source. The present study evaluated the protein quality of a selection of insect species. Insect substrates were housefly pupae, adult house cricket, yellow mealworm larvae, lesser mealworm larvae, Morio worm larvae, black soldier fly larvae and pupae, six spot roach, death's head cockroach and Argentinean cockroach. Reference substrates were poultry meat meal, fish meal and soyabean meal. Substrates were analysed for DM, N, crude fat, ash and amino acid (AA) contents and for in vitro digestibility of organic matter (OM) and N. The nutrient composition, AA scores as well as in vitro OM and N digestibility varied considerably between insect substrates. For the AA score, the first limiting AA for most substrates was the combined requirement for Met and Cys. The pupae of the housefly and black soldier fly were high in protein and had high AA scores but were less digestible than other insect substrates. The protein content and AA score of house crickets were high and similar to that of fish meal; however, in vitro N digestibility was higher. The cockroaches were relatively high in protein but the indispensable AA contents, AA scores and the in vitro digestibility values were relatively low. In addition to the indices of protein quality, other aspects such as efficiency of conversion of organic side streams, feasibility of mass-production, product safety and pet owner perception are important for future dog and cat food application of insects as alternative protein source.

Effects of preservation conditions of canine feces on in vitro gas production kinetics and fermentation end products1

This study investigated the effect of chilling and freezing (for 24 h) canine feces on in vitro gas production kinetics and fermentation end product profiles from carbohydrate-rich (in vitro run 1) and protein-rich (in vitro run 2) substrates. Feces were collected from 3 adult retriever-type dogs fed a canned diet for at least 2 wk. Each fecal sample was divided into 3 portions: 1 portion was used immediately as an inoculum (fresh) and the other 2 portions were used after either chilling to 5°C for 30 min and storage in crushed ice for 23.5 h (chilling) or freezing to -20°C for 30 min and storage in a prefrozen (-20°C) container for 23.5 h (freezing). The medium solution for run 1 contained N whereas that for run 2 was N free. Substrates included fructooligosaccharide (FOS), sugar beet pulp, and wheat middlings in run 1 and soybean meal, poultry meat meal, and feather meal in run 2. Gas production kinetics were calculated from cumulative gas production data measured for 72 h. After incubation, fermentation liquids were analyzed for short-chain fatty acids, NH3, and aromatic compounds. For both in vitro runs, chilling feces did not affect gas production kinetics and end product profiles of substrates compared with inocula from fresh feces. Freezing feces decreased the maximum rate of gas production in phase 2 for FOS (P<0.001) and across substrates increased gas produced (P≤0.005) and time of maximum gas production in phase 2 (P<0.001). Furthermore, compared with fresh fecal inocula, inocula from frozen feces resulted in increased overall indole concentrations in run 1 (P=0.006) and indole concentrations from soybean meal and poultry meat meal in run 2 (P<0.001). In run 2, phenol concentrations were greater (P=0.015) for frozen feces than for fresh feces (P=0.015). In conclusion, freezing canine feces for 24 h slightly altered fermentative characteristics of fecal inoculum whereas chilling feces in crushed ice for 24 h maintained fermentative characteristics. Chilling feces in crushed ice is a practical method to preserve feces during transport between laboratories within 24 h for in vitro fermentation studies evaluating dietary ingredients.

Apparent nutrient digestibility and mineral availability of protein-rich ingredients in extruded diets for Nile tilapia

Apparent digestibility coefficients (ADC) of gross energy and dry matter (DM) and mineral availability were evaluated for eight protein-rich ingredients (corn gluten meal, soybean meal, cottonseed meal at 280.0 and 380.0 g/kg, poultry by-product meal, meat meal, fish meal and feather meal) in compound diets for Nile tilapia. Chromic oxide was used as inert digestibility marker. Higher ADC values of DM and energy were, respectively, 0.862 and 0.881 for corn gluten meal, 0.811 and 0.888 for poultry by-product meal; 0.729 and 0.731 for feather meal; 0.666 and 0.754 for fish meal; 0.578 and 0.642 for soybean meal; 0.476 and 0.652 for meat meal at 450.0 g/kg; 0.352 and 0.449 for cottonseed meal at 380 g/kg; and 0.251 and 0.324 for cottonseed meal at 280.0 g/kg. The highest average mineral availability was for poultry by-product meal (0.582), and the lowest calcium and phosphorus availability were recorded for cottonseed meal at 280.0 g/kg. Therefore, poultry by-product meal appears to be the best ingredient for using in Nile tilapia diets.