Poultry Food Research Articles

Poultry Food Assess Risk Model for Salmonella and Chicken Gizzards: II. Illness Dose Step

The Illness Dose (ID) step of a Poultry Food Assess Risk Model (PFARM) for Salmonella and chicken gizzards (CGs) was shown in the present study. The illness dose is the minimum dose of Salmonella consumed that causes an illness. It depends on the zoonotic potential (ZP) of Salmonella, food consumption behavior (FCB), and consumer health and immunity (CHI) or the disease triangle (DT). Zoonotic potential is the ability of Salmonella to survive, grow, and spread in the production chain or food and then cause illness in humans. Illness dose is predicted in PFARM using a DT, dose-response model (DRM) that was developed with human feeding trial (HFT) data and was validated with human outbreak investigation (HOI) data for Salmonella. The ability of the DT, DRM to predict DR data from HOI and HFT for Salmonella was quantified using the Acceptable Prediction Zone (APZ) method where acceptable performance occurred when the proportion of residuals in the APZ (pAPZ) was ≥0.7. United States, Centers for Disease Control and Prevention (CDC) data for human salmonellosis from 2007 to 2016 were used to simulate ZP, and only minor changes in ZP of 11 Salmonella serotypes were observed during this time. The performance of the DT, DRM for predicting Salmonella DR data from HFT and HOI was acceptable with pAPZ that ranged from 0.87 to 1 for individual serotypes of Salmonella. Simulation results from the DT, DRM in PFARM indicated that ID decreased (P ≤ 0.05) and ZP increased (P ≤ 0.05) over time in the simulated production chain because the main serotype of Salmonella changed from Kentucky (low ZP) to Infantis (high ZP) while FCB and CHI were held constant. These results indicated that the DT, DRM in PFARM can be used with confidence to predict ID as a function of ZP, FCB, and CHI. In other words, the DT, DRM in PFARM can be used with confidence to predict dose-response for Salmonella and CGs.

Recent progress in pectin extraction and their applications in developing films and coatings for sustainable food packaging: A review

Perishable foods like fruits and vegetables, meat, fish, and dairy products have short shelf-life that causes significant postharvest losses, which poses a major challenge for food supply chains. Biopolymers have been extensively studied as sustainable alternatives to synthetic plastics, and pectin is one such biopolymer that has been used for packaging and preservation of foods. Pectin is obtained from abundantly available low-cost sources such as agricultural or food processing wastes and by products. This review is a complete account of pectin extraction from agro-wastes, development of pectin-based composite films and coatings, their characterizations, and their applications in food packaging and preservation. Compared to conventional chemical extraction, supercritical water, ultrasound, and microwave assisted extractions are a few examples of modern and more efficient pectin extraction processes that generate almost no hazardous effluents, and thus, such extraction techniques are more environment friendly. Pectin-based films and coatings can be functionalized with natural active agents such as essential oils and other phytochemicals to improve their moisture barrier, antimicrobial and antioxidant properties. Application of pectin-based active films and coatings effectively improved shelf-life of fresh cut-fruits, vegetables, meat, fish, poultry, milk, and other food perishable products.

Poultry as A Source and Reservoir for Campylobacteriosis

Gastrointestinal infection due to Campylobacter has been increasing throughout the world especially in developing countries where hygienic measures are compromising. In this review we have focused on the poultry role as source and reservoir for campylobacter infection compared with other meat sources. Contamination of Campylobacter starts from the surroundings of poultry flocks leading to its colonization subsequently transfer from poultry food chain to consumers through handling of raw or improperly cooked meat. Prevalence of Campylobacter is high in those regions where poultry production or consumption is high i.e., in European countries. Campylobacter infection has spread along with development of the poultry industry in the world as poultry is the main source of spread for Campylobacter species. Traveling to those areas where Campylobacter is prevalent is major risk factor for its spread. Increase in antimicrobial resistance in Campylobacter due to over use of medicines both in meat producing animals and humans is a disturbing situation for Public Health. Aim of intervention studies is to control colonization of Campylobacter at farm level that has proved to be promising outcomes in many countries. Proper biosecurity of farm and awareness programs for workers and consumers is necessary for control of infection. Proper batching, slaughtering, evisceration, packing, handling and cooking are also contributing factors to control of infection. Decreasing the over or unnecessary use of medicine is vital to avoid antimicrobial resistance in all types of bacteria. Although poultry is a major source along with some other meat for campylobacteriosis in humans but our surroundings i.e., untreated drinking water, sea food, direct contact with pets or commercial animals and some other sources that need to be studied are contributing to Campylobacter infection.

The Effect of Innovative Hydroponic Feed Technology on Poultry Performance in Private Farms

The research was carried out at the Scientific Research Veterinary Institute and in the subsidiary farms of the Khachmaz district (Azerbaijan). The article presents data on the development of poultry farming and food production. To develop a rational and proper feeding of poultry, the addition of antibiotic-effective plants with high nutritional qualities, to the feeding of chickens was used. This led to an increase in meat productivity, a reduction in feeding costs, and an improvement in productivity. Thus, an experiment was conducted by adding the antibiotic-effective plants of sorghum, licorice root, amaranth grain and hydroponically germinated corn, barley, amaranth and sorghum grain to the feed share of young birds to study the effect on meat productivity.

Effects of moldy corn on the performance, antioxidant capacity, immune function, metabolism and residues of mycotoxins in eggs, muscle, and edible viscera of laying hens

Mycotoxins, including aflatoxin B1 (AFB1), zearalenone (ZEN) and deoxynivalenol (DON), are common contaminants of moldy feeds. Mycotoxins can cause deleterious effects on the health of chickens and can be carried over in poultry food products. This study was conducted to investigate the effects of moldy corn (containing AFB1, ZEN, and DON) on the performance, health, and mycotoxin residues of laying hens. One hundred and eighty 400-day-old laying hens were divided into 4 treatments: basal diet (Control), basal diet containing 20% moldy corn (MC20), 40% moldy corn (MC40) and 60% moldy corn (MC60). At d 20, 40, and 60, the performance, oxidative stress, immune function, metabolism, and mycotoxin residues in eggs were determined. At d 60, mycotoxin residues in muscle and edible viscera were measured. Results showed the average daily feed intake (ADFI) and laying performance of laying hens were decreased with moldy corn treatments. All the moldy corn treatments also induced significant oxidative stress and immunosuppression, reflected by decreased antioxidase activities, contents of cytokines, immunoglobulins, and increased malonaldehyde level. Moreover, the activities of aspartate aminotransferase and alanine transaminase were increased by moldy corn treatments. The lipid metabolism was influenced in laying hens receiving moldy corn, reflected by lowered levels of total protein, high density lipoprotein cholesterol, low density lipoprotein cholesterol, total cholesterol, and increased total triglyceride as well as uric acid. The above impairments were aggravated with the increase of mycotoxin levels. Furthermore, AFB1 and ZEN residues were found in eggs, muscle, and edible viscera with moldy corn treatments, but the residues were below the maximum residue limits. In conclusion, moldy corn impaired the performance, antioxidant capacity, immune function, liver function, and metabolism of laying hens at d 20, 40, and 60. Moldy corn also led to AFB1 residue in eggs at d 20, 40, and 60, and led to both AFB1 and ZEN residues in eggs at days 40 and 60, and in muscle and edible viscera at d 60. The toxic effects and mycotoxin residues were elevated with the increase of moldy corn levels in feed.

False Beliefs about Contracting Avian (Bird) Flu from Processed Poultry Products

Avian influenza (bird flu) occurs sporadically in American poultry flocks, decimating these flocks and causing substantial economic losses. Avian influenza also impacts the beliefs of food handlers and preparers in the home (home cooks). Although those who properly handle and prepare processed poultry products cannot succumb to avian influenza, there is a widespread belief that one can contract the bird flu from these foods. Beliefs about getting avian influenza from poultry products and intentions to avoid consuming poultry products are the focus of this study of 285 home cooks. False beliefs about getting avian influenza from handling, preparing, and consuming poultry products are apparent in this sample. Correlational analysis also shows that those holding the false beliefs intend to act upon those beliefs by planning not to consume poultry products. Moreover, the false beliefs about contracting avian influenza from poultry products are correlated with a bias to see oneself as less likely to produce food that contains foodborne diseases. These findings are consistent with, and contribute to, the research literatures on belief formation and change, behavioral intentions, and with research showing how guilt by association thought processes underlie false beliefs related to food safety. This research has important implications for poultry and other food processing industries, and for campaigns to persuade the public about real and imaginary risks associated with particular food products.

Poultry Food Assess Risk Model for Salmonella and Chicken Gizzards: I. Initial Contamination

The Poultry Food Assess Risk Model (PFARM) project was initiated in 1995 to develop data collection and modeling methods for simulating the risk of salmonellosis from poultry food produced by individual production chains. In the present study, the Initial Contamination (IC) step of PFARM for Salmonella and chicken gizzards (CG) was conducted as a case study. Salmonella prevalence (Pr), number (N), and serotype/zoonotic potential (ZP) data (n = 100) for one sample size (56 g) of CG were collected at meal preparation (MP), and then Monte Carlo simulation (MCS) was used to obtain data for other sample sizes (112, 168, 224, 280 g). The PFARM was developed in Excel and was simulated with @Risk. Data were simulated using a moving window of 60 samples to determine how Salmonella Pr, N, and ZP changed over time in the production chain. The ability of Salmonella to survive, grow, and spread in the production chain and food, and then cause disease in humans was ZP, which was based on U. S. Centers for Disease Control and Prevention data for salmonellosis. Of 100 CG samples tested, 35 were contaminated with Salmonella with N from 0 to 0.809 (median) to 2.788 log per 56 g. Salmonella serotype Pr per 56 g was 16% for Kentucky (ZPmode = 1.1), 9% for Infantis (ZPmode = 4.4), 6% for Enteritidis (ZPmode = 5.0), 3% for Typhimurium (ZPmode = 4.9), and 1% for Thompson (ZPmode = 3.7). Results from MCS indicated that Salmonella Pr, N, and ZP among portions of CG at MP changed (P ≤ 0.05) over time in the production chain. Notably, the main serotype changed from Kentucky (low ZP) to Infantis (high ZP). However, the pattern of change for Salmonella Pr, N, and ZP differed over time in the production chain and by the statistic used to characterize it. Thus, a performance standard (PS) based on Salmonella Pr, N, or ZP at testing or MP will likely not be a good indicator of poultry food safety or risk of salmonellosis.

Phytate-Induced Dose-Response Auto-Activation of Enzyme in Commercial Recombinant Phytase From Escherichia coli.

Microbial phytase is one of the most widely used enzymes in food industries like cattle, poultry, and aquaculture food. Therefore, understanding the kinetic properties of the enzyme is very important to evaluate and predict its behavior in the digestive system of livestock. Working on phytase is one of the most challenging experiments because of some problems, including free inorganic phosphate (FIP) impurity in phytate (substrate) and interference reaction of the reagent with both phosphates (product and phytate impurity). In the present study, FIP impurity of phytate was removed, and then it was shown that the substrate (phytate) has a dual role in enzyme kinetics: substrate and activator. phytate impurity was decreased by two-step recrystallization prior to the enzyme assay. The impurity removal was estimated by the ISO30024:2009 method and confirmed by Fourier-transform infrared (FTIR) spectroscopy. The kinetic behavior of phytase activity was evaluated using the purified phytate as substrate by non-Michaelis-Menten analysis, including Eadie-Hofstee, Clearance, and Hill plots. The possibility of an allosteric site on phytase was assessed by molecular docking. The results showed a 97.2% decrease in FIP due to recrystallization. The phytase saturation curve had a sigmoidal appearance, and Lineweaver-Burk plot with a negative y-intercept indicated the positive homotropic effect of the substrate on the enzyme activity. A right-side concavity of Eadie-Hofstee plot confirmed it. Hill coefficient was calculated to be 2.26. Molecular docking also showed that Escherichia coli phytase molecule has another binding site for phytate very close to the active site, called "allosteric site". The observations strongly propose the existence of an intrinsic molecular mechanism in Escherichia coli phytase molecules to be promoted for more activity by its substrate, phytate (positive homotropic allosteric effect). In silico analysis showed that phytate binding to the allosteric site caused new substrate-mediated inter-domain interactions, which seems to lead to a more active conformation of phytase. Our results provide a strong basis for animal feed development strategies, especially in the case of poultry food and supplements, regarding a short food passage time in their gastrointestinal tract and variable concentration of phytate along with it. Additionally, the results strengthen our understanding of phytase auto-activation as well as allosteric regulation of monomeric proteins in general.

Prevalence and molecular characterization of typhoidal and non-typhoidal Salmonella isolated from meat and environmental samples of retail shops of Lahore Punjab, Pakistan.

Non-typhoidal Salmonellae are important foodborne bacterial pathogens that can cause bacteremia, gastroenteritis, and subsequent infection. The aim of the study was to determine the prevalence of Salmonella in the live bird market and retail shops of Lahore (Pakistan). A total of 720 samples of chicken meat, chopping board, cages, hands, and transportation vans were collected. Salmonella was recovered from 103 (14.36%) samples. The highest prevalence (33.33%) was found in transportation van samples followed by chicken meat samples (17.26%). In the towns of Lahore, the highest prevalence was found in Samanabad Town (19%) followed by Data Ganj Bakhsh Town (17%) with the lowest in Gulberg Town (6.9%). Salmonella Typhimurium was most common (35.92%) followed by S. Enteritidis (25.24%), S. Dublin (14.56%), S. Gallinarum biovar Gallinarum (8.74%), and untyped Salmonella species (15.53%). This was the first baseline study of the prevalence of non-typhoidal Salmonella at the live bird market and retail shops of Lahore. Implementation of appropriate control measures is required at both the human side and poultry food production chain to reduce the burden and transmission of the zoonotic Salmonellae.

Characteristic of multiple-antibiotic-resistant \(\textit{ Salmonella enteritica }\) from Muscovy duck in Hanoi

Nowadays, as the global population grows, the demand for food is also becoming higher each day. Together with the rise in food demand, Muscovy duck has been gradually bred industrially as a poultry food supply along with the chicken. The change from traditional to industrial breeding poses a potential risk of pathogenic bacteria infection and antimicrobial resistance bacteria. Especially Salmonella, one of the leading pathogens worldwide, is also notable for its antimicrobial resistance. In this study, by using Muscovy duck carcasses collected from wet markets in 05 districts in Ha Noi, we assessed the rate of Salmonella infection at first, then conducted an antibiotic susceptibility test utilizing 15 types of antibiotics, from then whole genome sequencing was applied for 8 multidrug resistant isolates. Next, the genomic data after successfully sequenced was used for analyzing antibiotic resistance genes, genotypes, multi-locus sequence-based typing (MLST), virulence factors, and plasmids. 65% of Muscovy duck samples were positive for Salmonella, in which 95% (19/20 strains) of Salmonella isolated was multidrug resistant. The result of the antibiotics susceptibility test indicated that phenotypic resistance to ampicillin was the most observed (92.3%, 19/20), followed by tetracycline (90%, 18/20), cefuroxime (85%, 17/20), cefazolin (85%, 17/20), ceftriaxone (85%, 17/20), Cefotaxime (85%, 17/20), trimethoprim (70%, 14/20), gentamicin (60%, 12/20), chloramphenicol (55%, 11/20), nalidixic acid (55%, 11/20), ceftazidime (50%, 10/20), ciprofloxacin (2/20). However, all isolates were susceptible to cefoxitin and meropenem. Sixty-five antibiotic resistance genes were identified, including genes that are resistant to aminoglycoside, 3rd generation antibiotics (cefotaxime, cefoperazone, ceftizoxime, ceftazidime, ceftriaxone, etc.). Col, IncA plasmids and some mobile genetic elements were identified. Simultaneously Salmonella pathogenic islands were found in all sequenced strains, exclusively SPI 1, SPI 3, and SPI 9 were carried in every isolate.

Effect of diets containing commercial bioactive compounds on Salmonella Heidelberg infection in broiler chicks.

Salmonella Heidelberg (SH) is responsible for economic losses in poultry farming and food infections in humans and is a serious public health problem. Recently, there has been an increase in the frequency of isolation of this serotype in batches of broilers raised in Brazil. It is necessary to find new ways to help control this pathogen. The present study aimed to evaluate the effect of diets containing the compound Original XPC, which is a prebiotic-like fermented compound (PFC), and/or Sangrovit, which is a sanguinarine-based phytobiotic (SAN), on SH infection in broiler chicks. For this purpose, SH colonization in the cecum and its invasion into the spleen and liver were evaluated, as were the histopathological changes caused in these organs. The lowest cecal SH counts were observed in birds that ingested SAN, followed by those fed PFC (P < 0.05), with no added effect when the two bioproducts were used together (SAN + PFC). The mean SH and liver spleen counts did not differ between groups (P > 0.05). In general, birds from all groups challenged with SH showed similar macroscopic changes, such as hemorrhagic areas, hepatomegaly, and splenomegaly, such changes being more intense in the infected control group. The microscopic changes observed in the liver included hepatocyte congestion, heterophil infiltration in the sinusoid capillaries, areas of necrosis, and mononuclear inflammation. In the cecum, heterophilic infiltrate and thickening of the lamina propria were observed. In the ileum, the most common changes were congestion and thickening of the lamina propria and atrophy of the villi and crypts. The microscopic changes were less intense in the supplemented birds than the infected control group, and those supplemented with SAN developed the least changes. As ideal conditions for histomorphometric parameters of the ileum, the villus:crypt ratio in birds should be high, the villi should be long, and the crypts should be shallow. In the present study, higher mean heights and villus areas were observed in uninfected control and SAN group birds, and the crypt depth was lower in birds in the negative control group. The lowest villus:crypt ratio was observed in the birds of the infected control group. Although additional studies are needed, the preliminary results of the current investigation indicated that the addition of bioproducts, especially SAN, to the diet of birds helped to control SH infection, reducing its count in the cecum and improving overall and intestinal health.

Olive Leaf as a Source of Antibacterial Compounds Active against Antibiotic-Resistant Strains of Campylobacter jejuni and Campylobacter coli.

Campylobacter spp. are the main cause of bacterial gastroenteritis worldwide, and broiler chicks are the main vector of transmission to humans. The high prevalence of Campylobacter in poultry meat and the increase of antibiotic resistant strains have raised the need to identify new antimicrobial agents. For this reason, the aim of the current study was to evaluate the antibacterial activity of two extracts of olive leaf against antibiotic-resistant Campylobacter strains (C. jejuni and C. coli) isolated from poultry food chain. The extracts of olive leaf (E1 and E2) were markedly different in their chemical compositions. While E1 was composed predominantly of highly hydrophilic compounds such as hydroxytyrosol and hydroxytyrosol glucosides (14,708 mg/100 g), E2 mainly contained moderately hydrophilic compounds, with oleuropein (20,471 mg/100 g) being prevalent. All Campylobacter strains exhibited similar antibiotic profiles, being resistant to ciprofloxacin and tetracycline. E1 showed strong antibacterial activity and reduced bacterial growth from 4.12 to 8.14 log CFU/mL, depending on the strain. Hydroxytyrosol was the main compound responsible, causing the inhibition of growth of Campylobacter strains at low concentrations (0.1-0.25 mg/mL). E2 demonstrated a lower antibacterial effect than E1, reducing growth from 0.52 to 2.49 log CFU/mL. The results of this study suggest that the optimization of the composition of olive-leaf extracts can provide improved treatment results against Campylobacter strains.

Buffered Peptone Water Formulation Does Not Influence Growth of pESI-positive Salmonella enterica Serovar Infantis

Salmonella enterica is a major cause of human foodborne illness and is often attributed to poultry food sources. S. enterica serovar Infantis, specifically those carrying the pESI plasmid, has become a frequently isolated serotype from poultry meat samples at processing and has caused numerous recent human infections. In 2016, the USDA-Food Safety and Inspection Service changed the official sampling method for raw poultry products from BPW to using neutralizing BPW (nBPW) as the rinsing agent in order to prevent residual antimicrobial effects from acidifying and oxidizing processing aids. This change was contemporaneous to the emergence of pESI-positive ser. Infantis as a prevalent serovar in poultry, prompting some to question if nBPW could be selecting for this prevalent serovar. We performed two experiments: a comparison of ser. Infantis growth in BPW versus nBPW, and a simulation of regulatory sampling methods. We found that when inoculated into both broths, ser. Infantis initially grows slightly slower in nBPW than in BPW but little difference was seen in abundance after 6 h of growth. Additionally, the use of nBPW to simulate poultry rinse sample and overnight cold shipping to a regulatory lab did not affect the survival or subsequent growth of ser. Infantis in BPW. We concluded that the change in USDA-FSIS methodology to include nBPW in sampling procedures has likely not affected the emergence of S. ser. Infantis as a prevalent serovar in chicken and turkey meat product samples.

Characteristics, pathogenic mechanism, zoonotic potential, drug resistance, and prevention of avian pathogenic Escherichia coli (APEC).

Although most Escherichia coli (E. coli) strains are commensal and abundant, certain pathogenic strains cause severe diseases from gastroenteritis to extraintestinal infections. Extraintestinal pathogenic E. coli (ExPEC) contains newborn meningitis E. coli (NMEC), uropathogenic E. coli (UPEC), avian pathogenic E. coli (APEC), and septicemic E. coli (SEPEC) based on their original host and clinical symptom. APEC is a heterogeneous group derived from human ExPEC. APEC causes severe respiratory and systemic diseases in a variety of avians, threatening the poultry industries, food security, and avian welfare worldwide. APEC has many serotypes, and it is a widespread pathogenic bacterium in poultry. In addition, ExPEC strains share significant genetic similarities and similar pathogenic mechanisms, indicating that APEC potentially serves as a reservoir of virulence and resistance genes for human ExPEC, and the virulence and resistance genes can be transferred to humans through food animals. Due to economic losses, drug resistance, and zoonotic potential, APEC has attracted heightened awareness. Various virulence factors and resistance genes involved in APEC pathogenesis and drug resistance have been identified. Here, we review the characteristics, epidemiology, pathogenic mechanism zoonotic potential, and drug resistance of APEC, and summarize the current status of diagnosis, alternative control measures, and vaccine development, which may help to have a better understanding of the pathogenesis and resistance of APEC, thereby reducing economic losses and preventing the spread of multidrug-resistant APEC to humans.

Hazards In Poultry Feed Production: An Appraisal From The Halal Perspective

Animal feed is any processed, semi-processed of raw materials or compound which are fed to animals. Poultry feed are food for farm poultry, including chickens, ducks, geese and other domestic birds (Gallus domesticus), whether it is in mash, crumbles or pellets form. Feed supply is central to all animal production systems and any factor that affects the security of the feed supply is a significant constraint to production. In Malaysia, most of the poultry feed comprises of 95% of the sources that are imported and only 5% are obtained locally. In this situation, feed producers are responsible to communicate with crop producers and at the same time connected with primary food producers involved, such as poultry breeders. These phenomena are crucial in maintaining the halal integrity along the supply chain. To produce halal food materials, it is important to make sure that the feed fed to edible animals are Ḥalālan ṭoyyiban. This paper reviews the hazards presence in poultry feed and its production which include biohazards, chemical hazards and physical hazards. In so doing, it employs library research methodology by reviewing the relevant literatures and analysed using content analysis method. Further to that, it also appraises the presence of the respective hazards from the halal perspective. From the halal point of view, the sources of human food shall not only be halal, but shall also be safe for human consumption, non-poisonous and non-hazardous to human health. In the context of poultry feed, the usage of antibiotics, GMOs with hazardous issue or potential toxicity or allergenicity, toxin contamination and presence of physical contaminants in poultry feed, if not properly controlled and regulated seriously, adverse effects might take place in human and this is indeed not in line with the goal of the Shariah to safeguard the interests of human and the universe, to protect life and lineage, and to bring benefits and repelling harms. Nevertheless, considering that contaminants are present everywhere, and that total elimination is almost impossible, taking proactive actions to reduce their presence to the minimum level possible is in compliance with the spirit of Maqasid Shariah. This could be done by putting in place a comprehensive legislation for the control of several of these chemical compounds, pathogens and physical contaminants in poultry feed, or by implementing feed safety management system such as HACCP in feed plants. In addition, to protect the interest of the Muslim consumers particularly in Malaysia, it is also recommended that a Halal standard for Poultry Feed Production should be developed.

Antibacterial potency of Vernonia amygdalina on antibiotic-resistant non-typhoidal salmonella isolates from human and animal sources in southern Taraba, North-East, Nigeria

Antibiotic discovery was considered a wonder of the world. Shortly after, many microorganisms began to exhibit resistance to prior antimicrobial actions of antibiotics. Notwithstanding, Quinones, Flavonoids, Flavones, Flavonols, Tannins, and Coumarins are major phytochemicals synthesized by plant parts and have shown decisive antimicrobial activities against a panel of microorganisms. In this research hot and cold extracts of fresh and dried Vernonia, amygdalina showed remarkable in vivo antimicrobial activity on antibiotic-resistant Salmonella isolates from various poultry farms and food vendors in Ibi, Donga, Takum, and Wukari. 5 g-10 ml, 10 g-10 ml, 15g-10ml, 20g-10ml, and 25g-10ml are arrays of Vernonia Amygdalina concentrations tested on the inocula. Significant light growth was observed for both menstruum extracts of dried and fresh leaves of Vernonia Amygdalina at higher concentrations levels of 20g-10ml, 25g-10ml, while and moderate growth was seen at lower concentrations levels of 5g-10ml, 10g-10ml. Also, heavy growth of Salmonella was detected in the control plates inoculated with the test microorganism but without the addition of any extracts. Weak political will in implementing regulation policies, funding of research, and perceived antimicrobial resistance to plant extracts as well as acceptability and cultural factors have threatened the extensive use of plant extract in the management of health. Improved universal health coverage and enhanced economic potentials are core benefits of the use of plant extracts for health management. Conclusively, plant extracts possess appreciable antimicrobial activities against Multi-Drug Resistance (MDR) microorganisms compared to currently prescribe synthetic drugs. Hence, further studies on clinical efficacy trials and the safety of more plants need to be intensified.

Phase Angle Association with Dietary Habits and Metabolic Syndrome in Diabetic Hypertensive Patients: A Cross-Sectional Study.

Phase angle (PhA) levels are often lower than normal because both disease-specific parameters and disease-related inflammatory status, metabolic syndrome (MetS) included, can affect PhA. Therefore, the aim of this cross-sectional study was to compare body composition, metabolic profile and dietary patterns of participants with arterial hypertension (AH), type 2 diabetes mellitus (T2DM) and MetS with regard to PhA values. A total of 208 participants were included, of whom 53.6% were males. For each participant, data about body composition and anthropometric parameters, clinical and laboratory parameters, as well as food frequency questionnaire (FFQ) and Mediterranean Diet Serving Score (MDSS) were obtained. MC-780 Multi Frequency Segmental Body Mass Analyzer (Tanita) was used to assess body composition. Furthermore, waist-to-hip ratio (WHR) and waist-to-height ratio (WHtR) were calculated. The results showed that 75 (36.06%) participants had low PhA values and 133 (63.94%) had high PhA values. Participants with higher PhA values had significantly higher body fat percentage (p = 0.04), fat-free mass (kg; p < 0.001), muscle mass (kg; p < 0.001), skeletal muscle mass (% and kg; p < 0.001), sarcopenic index (SMI; p < 0.001) and mid-upper arm circumference (MUAC; p = 0.04), as well as lower fat mass percentage (p = 0.04). Regarding food frequency consumption, significantly higher intakes of red meat (p = 0.003), poultry (p = 0.02) and fast food (p = 0.003) were noticed in participants with higher PhA values. Adherence to the Mediterranean Diet (MeDi) was exceptionally low in both groups of participants, with significantly higher fish intake noticed in participants with high PhA (p = 0.03). In conclusion, our results showed that body composition could be the indicator of PhA in MetS as well as overall low adherence to the MeDi principles. These findings highlight the importance of adequate nutritional strategies and novel approaches to maintaining optimal body composition and adopting proper eating habits within the framework of one’s disease.

Sublethal effects of pesticide residues differ between strains of captive Grey partridge: Consequences in terms of rearing conditions

Over the last 50 years, farmland bird populations have declined steeply in Europe and North America. Reintroduction or reinforcement for populations unable to self-maintain are popular management tools to overcome extinction risk through captive rearing and release. However, released birds tend to have lower survival rates than their wild conspecifics. Here, we aimed to mimic the diet shift that Grey partridge (Perdix perdix) encounters once released (poultry food to natural grain) and compared (i) differences in responses to this transition depending on strain (wild or farm) and (ii) the consequences of ingesting organic or conventional grains on individual body mass, haematocrit and behavioural traits (anti-predator responses, activity, exploration) that are likely critical for post-release survival. Our experimental procedure involved 40 farm-strain and 40 wild-strain partridges fed with commercial poultry food during early life then with wheat and corn for 3 months, from either organic or conventional (clopyralid residues) agriculture. The results suggest that the haematocrit and body mass index of farm-strain partridges were lower at the end of the experiment, probably due to diet shift. Birds fed conventional grains (with clopyralid residues) exhibited lower flight duration and flight initiation distance, and were more likely to translocate by running rather than flying. The findings suggest that, when rearing partridges of farm-strain origin, birds fed organic grains experience better post-release survival than birds fed conventional grains containing pesticide residues.

Antimicrobial Resistance Profile of Common Foodborne Pathogens Recovered from Livestock and Poultry in Bangladesh.

Multidrug-resistant (MDR) foodborne pathogens have created a great challenge to the supply and consumption of safe & healthy animal-source foods. The study was conducted to identify the common foodborne pathogens from animal-source foods & by-products with their antimicrobial drug susceptibility and resistance gene profile. The common foodborne pathogens Escherichia coli (E. coli), Salmonella, Streptococcus, Staphylococcus, and Campylobacter species were identified in livestock and poultry food products. The prevalence of foodborne pathogens was found higher in poultry food & by-product compared with livestock (p &lt; 0.05). The antimicrobial drug susceptibility results revealed decreased susceptibility to penicillin, ampicillin, amoxicillin, levofloxacin, ciprofloxacin, tetracycline, neomycin, streptomycin, and sulfamethoxazole-trimethoprim whilst gentamicin was found comparatively more sensitive. Regardless of sources, the overall MDR pattern of E. coli, Salmonella, Staphylococcus, and Streptococcus were found to be 88.33%, 75%, 95%, and 100%, respectively. The genotypic resistance showed a prevalence of blaTEM, blaSHV, blaCMY, tetA, tetB, sul1, aadA1, aac(3)-IV, and ereA resistance genes. The phenotype and genotype resistance patterns of isolated pathogens from livestock and poultry had harmony and good concordance, and sul1 & tetA resistance genes had a higher prevalence. Good agricultural practices along with proper biosecurity may reduce the rampant use of antimicrobial drugs. In addition, proper handling, processing, storage, and transportation of foods may decline the spread of MDR foodborne pathogens in the food chain.

Assessing evidence of a potential Salmonella transmission across the poultry food chain.

Enhanced Salmonella surveillance programmes in poultry were implemented in all European Member States, with minimum prevalence targets for a list of targeted serotypes to safeguard food and public health. Based on the Belgian Salmonella surveillance programme and focusing on poultry, the overarching aim of this study was to highlight possible Salmonella transmissions across the food chain (FC). For this purpose, firstly, the prevalence patterns of Salmonella (targeted and the most prevalent non-targeted) serotypes along the FC were described over time. Secondly, the effectiveness of the control measures against vertical transmission (breeders to 1-day-old broiler and layer chicks) was indirectly assessed by looking into the odds of targeted serotypes detection. Thirdly, it was appraised if Salmonella prevalence can significantly increase during broilers and layers production. In addition, it was tested if being tested negative at the end of production in broilers when tested positive at the entrance is serotype dependent (targeted vs. non-targeted serotypes). Results showed that, firstly, the prevalence patterns of the listed serotypes were inconstant over time and across the FC. Secondly, the odds of Salmonella targeted serotype detection in 1-day-old broiler and in 1-day-old layer flocks were lower than in breeder flocks while, thirdly, infection during broiler and layer production can lead to significant increase in positivity in subsequent samples. Finally, being infected by a targeted or by non-targeted serotype at the entrance of the flock poorly reflects the Salmonella status at the end of production. Note that this study did not make a distinction between the different sources of contamination and the effects of sampling methods and isolation methods should be subject to further investigation.