Fuel cell electric vehicles (FCEVs) powered by proton exchange membrane fuel cells (PEMFCs) provide a sustainable, low-carbon alternative to the world’s current energy structure based on fossil fuels. At the heart of this solution, the PEMFC technology is met with two main barriers for wide commercialization: the cost and degradation of electrocatalysts for the oxygen reduction reaction (ORR) [1]. The main thrusts for ORR electrocatalyst development are currently Pt-transition metal (TM) alloy nanoparticles [1] and atomically-dispersed platinum group metal-free (PGM-free) catalysts [2] that reduce the Pt loading or replace Pt completely.The degradation of Pt-TM alloy nanoparticles, such as PtCo, involves the combined processes of Pt and Co dissolution, Pt oxide formation, and Pt redeposition. The interplay between different properties of catalysts, such as particle size distribution (PSD), Co composition, Pt-skin surface strain, and carbon support pore structure complicates attempts to link degradation mechanisms with loss in ORR activity and overall fuel cell performance [1]. Further, inhomogeneities of the catalyst layer limit the ability for electron microscopy to analyze the degradation process on the nanoscale. Similarly, the study of PGM-free catalyst degradation is also met with inhomogeneities which stem from the complex morphology of graphitic carbon particles, wide range of defects, and plurality of Fe active sites [2, 3].Identical-location scanning transmission electron microscopy (IL-STEM) allows the same catalytic material to be studied before and after ex situ cycling, minimizing the influence of inhomogeneities and therefore providing clearer insight into degradation mechanisms at the nanoscale [4]. Since the specimen is identical to a conventional STEM specimen, imaging and analytical methods like energy-dispersive X-ray spectroscopy and electron energy-loss spectroscopy can be performed at high spatial resolution. IL-STEM has previously been used to elucidate Pt catalyst degradation mechanisms such as migration, coalescence, and detachment from the carbon support [5-7]. Ostwald ripening has not been observed in most IL-STEM studies [5-7], however, in stark contrast to membrane electrode assembly (MEA) tests where it is identified as a primary degradation mechanism [8, 9]. Therefore, reproducing degradation processes that occur in MEAs, which is critical for accurately identifying mechanisms that can inform mitigation strategies, is still a challenge for IL-STEM.In this work, we seek to identify IL-STEM experimental conditions and protocols that produce end of life (EOL) catalyst morphology and composition that mimic those found in MEAs. A range of parameters are explored, such as specimen preparation method, electrolyte concentration, gaseous environment, upper and lower cycling potentials, and potential wave shape to reproduce the catalyst degradation observed in both Pt-based and PGM-free MEAs. The contribution of different degradation mechanisms revealed by IL-STEM in each system will be presented, including particle coalescence, Ostwald ripening, TM leaching, and carbon corrosion. The resulting insights into fundamental fuel cell catalyst degradation mechanisms will be used to guide development of catalysts with enhanced performance and durability. [10]References[1] RL Borup et al., Curr. Opin. Electrochem. 21 (2020), p. 192.[2] L Osmieri et al., Curr. Opin. Electrochem. 25 (2021), p. 100627.[3] T Asset et al., Joule 24 (2020), p.33.[4] N Hodnik et al., Curr. Opin. Electrochem. 15 (2019), p. 73.[5] K Schlogl et al., J. Electroanal. Chem. 662 (2011), p. 355.[6] Y Yu et al., Nano Lett. 12 (2012), p. 4417.[7] L Dubau et al., Electrochim. Acta 110 (2013), p. 273.[8] H Yu et al., Electrochim. Acta 247 (2017), p. 1169.[9] TE O’Brien et al., ECS Trans. 98 (2020), p. 505.[10] This work was supported by the U.S. Department of Energy (DOE), Energy Efficiency and Renewable Energy, Fuel Cell Technologies Office under the M2FCT and ElectroCat 2.0 consortia. Electron microscopy was conducted at the Center for Nanophase Materials Sciences, which is a DOE Office of Science User Facility.This manuscript has been authored by UT-Battelle, LLC under Contract No. DE-AC05-00OR22725 with the U.S. Department of Energy. The United States Government retains and the publisher, by accepting the article for publication, acknowledges that the United States Government retains a non-exclusive, paid-up, irrevocable, world-wide license to publish or reproduce the published form of this manuscript, or allow others to do so, for United States Government purposes. The Department of Energy will provide public access to these results of federally sponsored research in accordance with the DOE Public Access Plan (http://energy.gov/downloads/doe-public-access-plan).