Improving suction technology for nerve activity recording

Abstract

BackgroundExtracellular recording of nerve activities using suction electrodes is an easy yet powerful tool in characterizing neural activities in physiology and pathological conditions. The key factors that determine the quality of suction electrode recordings have not been fully investigated.New Methods: Here, we proposed a biophysical model to study the mechanisms underlying suction technology for axon recording. The model focuses on the interpretation of the recorded single neuron activity based on the location of the electrode, the integrity of the recorded tissue, and the tightness of the suction. To directly test these model predictions, we applied two channel recordings from the nerves in Aplysia californica, and analyzed the shape of the extracellularly recorded single neuron activity under various conditions. ResultsWe found that both the recording site and the integrity of the neural tissue impact the shape of the action potentials traveling along the axon. In practice, the tightness of the suction is the key parameter for high-quality recordings using a suction electrode.Comparison with Existing Methods: Experimental protocols that can improve precise positioning of the electrode tip to the target nerve, avoid tissue damage, enhance suction force, and maintain tightness are essential for high-quality suction recording from axons. Current methods have not emphasized on achieving and maintaining of the suction pressure during experimentation, and have sometimes ignored the impact of suction electrode position or tissue damage to the quality of the recorded neural signal. ConclusionsA combined theoretical analysis and experimental approach is essential in improving neural recording technology. The work provides theoretical and practical guidelines to improve suction technology. This work also provides valuable insights to the improvement of several other extracellular recording technology in laboratory research or clinical settings.