Production Potential Research Articles

D-Arabitol production by a high arabitol-producing yeast, Zygosaccharomyces sp. Gz-5 isolated from miso.

d-Arabitol, an alternative sweetener to sugar, has low calorie content, high sweetness, low glycemic index, and insulin resistance-improving ability. In this study, d-arabitol-producing yeast strains were isolated from various commercial types of miso, and strain Gz-5 was selected among these strains. Phylogenetic tree analysis of the internal transcribed spacer sequence revealed that strain Gz-5 was distinct from Zygosaccharomyces rouxii, a major fermenting yeast of miso. The strain, identified as Zygosaccharomyces sp. Gz-5, grew better than other Z. rouxii in 15 g/L NaCl and produced 114 g/L d-arabitol from 295 g/L glucose in a batch culture for 8 days (0.386 g/g-consumed glucose). In a fed-batch culture, the yeast produced 133 g/L d-arabitol for 14 days, and the total d-arabitol amount increased by 1.75-fold. These results indicated that Zygosaccharomyces sp. Gz-5, a non-genetically modified strain, has excellent potential for the industrial production of d-arabitol.

Pt1.8Pd0.2CuGa Intermetallic Nanocatalysts with Enhanced Methanol Oxidation Performance for Efficient Hybrid Seawater Electrolysis.

Seawater electrolysis is a potentially cost-effective approach to green hydrogen production, but it currently faces substantial challenges for its high energy consumption and the interference of chlorine evolution reaction (ClER). Replacing the energy-demanding oxygen evolution reaction with methanol oxidation reaction (MOR) represents a promising alternative, as MOR occurs at a significantly low anodic potential, which cannot only reduce the voltage needed for electrolysis but also completely circumvents ClER. To this end, developing high-performance MOR catalysts is a key. Herein, a novel quaternary Pt1.8Pd0.2CuGa/C intermetallic nanoparticle (i-NP) catalyst is reported, which shows a high mass activity (11.13 A mgPGM -1), a large specific activity (18.13mA cmPGM -2), and outstanding stability toward alkaline MOR. Advanced characterization and density functional theory calculations reveal that the introduction of atomically distributed Pd in Pt2CuGa intermetallic markedly promotes the oxidation of key reaction intermediates by enriching electron concentration around Pt sites, resulting in weak adsorption of carbon-containing intermediates and favorable adsorption of synergistic OH- groups near Pd sites. MOR-assisted seawater electrolysis is demonstrated, which continuously operates under 1.23V for 240h in simulated seawater and 120h in natural seawater without notable degradation.

Nicotiana benthamiana as a potential source for producing anti-dengue virus D54 neutralizing therapeutic antibody

Dengue virus (DENV), transmitted by mosquitoes, is classified into four serotypes (DENV1-4) and typically causes mild, self-limiting symptoms upon initial infection. However, secondary infection can lead to severe symptoms due to antibody-dependent enhancement (ADE). To address this, anti-DENV antibodies are being developed with the goal of neutralizing infection without ADE activity. Previous attempts using a 54_hG1 antibody from CHO-K1 mammalian cells resulted in ADE induction, increasing viral infection. This study aimed to express the D54 monoclonal antibody in Nicotiana benthamiana. The plant-produced antibody had a similar neutralizing profile to the previous 54_hG1 antibody. Notably, the ADE activities of the plant-derived antibody were successfully eliminated, with no sign of viral induction. These findings suggest that N. benthamiana could be a source of therapeutic DENV antibodies. The method offers several advantages, including lower ADE, cost-effectiveness, simple facility requirements, scalability, and potential industrial-scale production in GMP facilities.

LED blue light enhances the accumulation and synthesis of steroidal alkaloids in Fritillaria unibracteata Hsiao et K. C. Hsia in vitro

The unique light environment of alpine regions exerts significant influences on the growth, development, and metabolism of alpine plants, with light quality being a fundamental component of light conditions. Varied light qualities manifest diverse effects on plant physiology and function. In order to investigate the impact of light quality on F. unibracteata, this study conducted a comparative analysis of six distinct light quality treatments on the growth, development, stress tolerance, and bioactive compound content of isolated seedlings, and to determine the biomass, physiological parameters, and concentrations of medicinal constituents. It was showed that the blue light treatment was beneficial to F. unibracteata to maintain stable plant growth and increase the total alkaloid content. Furthermore, we explored the effects of blue light treatment on the growth and major medicinal components of F. unibracteata using transcriptome sequencing technology. Through DEGs analysis, we explain the mechanism by which F. unibracteata responds to blue light and that blue light promotes alkaloid accumulation by up-regulating the MEP pathway in the steroidal alkaloid synthesis pathway. These findings suggest that optimizing light conditions can regulate F. unibracteata growth, development, and active ingredient content, laying the groundwork for potential large-scale production.

Stability, Characterization, and Antibacterial Activity of Cinnamon (Cinnamomum burmanii) Extract Mask Preparations as Potential Skin Care Products

Stability, Characterization, and Antibacterial Activity of Cinnamon (Cinnamomum burmanii) Extract Mask Preparations as Potential Skin Care Products

Effects of simulated nitrogen deposition on BVOCs emission dynamics and O3 and SOA production potentials in seedlings of three Ficus species

Nitrogen deposition affects the emission of biogenic volatile organic compounds (BVOCs) and thus their formation of ozone (O3) and secondary organic aerosols (SOA). The present study employed four nitrogen concentrations (6, 10, 15, and 30 kg ha−1 yr−1) and two nitrogen application methods (foliar surface and root application) to investigate the short-term effects of nitrogen deposition on BVOC emissions in seedlings of Ficus virens, Ficus concinna and Ficus elastica through controlled indoor pot experiments. The results demonstrated a positive correlation between the nitrogen concentration and the emission rate of BVOCs, with leaf nitrogen application exhibiting a significantly greater impact than root nitrogen application. The net photosynthetic rate and stomatal conductance emerged as pivotal factors influencing the emission of BVOCs. The maximum incremental reactivity (MIR) and fractional aerosol coefficient (FAC) methods were employed to assess the contribution of BVOCs to O3 formation and SOA production. Our findings indicate that isoprene emitted by seedlings from the three plant species emerged as the predominant driver for O3 formation, while monoterpenes and sesquiterpenes played a pivotal role in SOA production.

Rapid purification and scale-up of tilianin using counter-current chromatography with rectangular horizontal tubing

In counter-current chromatography (CCC), linear scale-up is an ideal amplification strategy. However, when transferring from analytical to predictable preparative processes with high throughput, linear scale-up would be challenging due to limitations imposed by differences in instrument parameters, such as gravitational forces, tubing cross-section area, tubing length, column volume and flow rate. Some effective scale-up strategies have been studied for different instrument parameters, but so far, these scale-up works have only been tested on standard circular (SC) tubing.The previous research of our group found that rectangular horizontal (RH) tubing can double the separation efficiency compared with conventional SC tubing, and has industrial production potential. This paper used the separation of tilianin from Dracocephalum moldavica L. as an example to demonstrate how to scale up the optimized process from analytical SC tubing to preparative RH tubing. After systematic optimization of solvent systems, sample concentration and flow rate on the analytical CCC, the optimized parameters obtained were successfully transferred to the preparative CCC. The results showed that a crude sample of 2.07 g was successfully separated using a solvent system of n-hexane - ethyl acetate - ethanol - water (1:4:1:5, v/v/v/v) in reversed phase mode, and the three consecutive separations produced a total of 380 mg tilianin in 75 min with high purities of 98.3%, as analyzed by HPLC. The total throughput achieved from the analytical to semi-preparative scale was improved by 138 times (from 12 mg/h to 1.66 g/h), while the column volume was increased by only 46.5 times (from 15.5 mL to 720 mL). This is the successful application of CCC for the separation and purification of tilianin. Given that SC tubing is the traditional configuration for CCC columns, this study is a necessary step to prove the applicability of RH tubing columns for routine use and potential large-scale industrial applications.

Экологическая стабильность и пластичность сортов горчицы сарептской яровой селекции ВНИИМК

Analysis of ecological plasticity and stability must be a base in the development of new genotypes of spring brown mustard. The purpose of the research was a comparative characteristic of spring brown mustard cultivars bred at the V.S. Pustovoit All-Russian Research Institute of Oil Crops by calculated statistic parameters of adaptability, ecological plasticity, stability, stress-resistance, and genetic flexibility in the central zone of the Krasnodar region in 2018–2022. We studied commercial (Nika, Yunona, Gorlinka, Galateya) and promising (VN11/18, VN-27/18, VN-9/18, and VN-25/18) cultivars. The indicators of ecological plasticity and stability were calculated basing on seed yield of spring brown mustard cultivars for five years by a methodology of Eberhart S.A., Rassell W.A. as reworded by Zykin V.А. To calculate a coefficient of a linear regression, we determined environment indexes, which characterized variability of conditions of the cultivar cultivation. The stress-resistance and genetic flexibility were determined by an equation of Rossille A.A., Hamblin J. as presented by Goncharenko А.А. The potential productivity and adaptability were revealed by a methodology of Zhivotkov L.А. The weather conditions in 2020 and 2021 were the most favorable, environmental index had the highest positive meaning and was equal to 1.74 and 1.82 under the highest average yield of brown mustard, which was equal to 1.92 and 1.93 t/ha, respectively. The weather conditions of 2018 and 2022 were less favorable for estimation of brown mustard samples (Ij = -1.71 and -2.00). A comparative analysis of brown mustard cultivars bred at the V.S. Pustovoit All-Russian Research Institute of Oil Crops by parameters of ecological plasticity and stability in the Krasnodar region showed the cultivars Yunona, VN-27/18, and VN-11/18 had the highest plasticity, and Yunona and Galateya demonstrated the highest stability and adaptability to declining of cultivation conditions. Thus, the cultivars Yunona and Galateya were characterized with high yield, plasticity, stability, genetic flexibility, and adaptability. In contrast conditions, the promising cultivar VN-9/18 had high indicators of resistance to stress factors, yield, and adaptability coefficient.

Natural-inspired hierarchic double-Janus solar evaporator for stable clean water production from high-salinity emulsions

Interfacial solar evaporation shows great potential in clean water production, emulsions separation, and high-salinity brine treatment. However, it remains challenging for the evaporators to maintain a high evaporation rate in the high-salinity emulsions due to the co-pollution of salt and oil. Herein, we first proposed a hierarchic double-Janus solar evaporator (HDJE) with a hydrophobic salt-rejecting top layer and oil-rejecting bottom layer. Compared to the traditional one, HDJE could treat industrial high-salinity oil-in-water emulsions stably for over 70 h, with a stable average evaporation rate of 1.73 kg m−2 h−1 and a high purification efficiency of up to 99.8 % for oil and ions. It was also verified that HDJE could be used for high-efficiency purification of oily concentrated seawater outdoor. An average water production rate of 3.59 kg m−2 d−1 and a TOC removal ratio of over 98 % was obtained. In conclusion, this study provides a novel way to effectively dispose of high-salinity oily wastewater.

The Emerging Role of Natural Products in Cancer Treatment.

The exploration of natural products as potential agents for cancer treatment has garnered significant attention in recent years. In this comprehensive review, we delve into the diverse array of natural compounds, including alkaloids, carbohydrates, flavonoids, lignans, polyketides, saponins, tannins, and terpenoids, highlighting their emerging roles in cancer therapy. These compounds, derived from various botanical sources, exhibit a wide range of mechanisms of action, targeting critical pathways involved in cancer progression such as cell proliferation, apoptosis, angiogenesis, and metastasis. Through a meticulous examination of preclinical and clinical studies, we provide insights into the therapeutic potential of these natural products across different cancer types. Furthermore, we discuss the advantages and challenges associated with their use in cancer treatment, emphasizing the need for further research to optimize their efficacy, pharmacokinetics, and delivery methods. Overall, this review underscores the importance of natural products in advancing cancer therapeutics and paves the way for future investigations into their clinical applications.

Dynamics of variation of dairy characteristics in cows when using the feed additive “VivAktiv”

Relevance. Holstein dairy cattle are bred in the Sverdlovsk region, its improvement is carried out by selecting and selecting the best for further breeding. In order for them to display their genetic potential of productivity, it is necessary to ensure a balanced diet. The aim of the work was to assess the diversity of dairy characteristics in Holstein cows when using the “VivAktiv” feed additive.Methods. To conduct the study, 4 groups of calved heifers with 10 heads each were formed in a typical breeding plant for the region: a control group and three experimental ones based on the principle of pairs of analogues. The dairy productivity of cows was evaluated for the first lactation and for the calendar year by control milking once a month. The fat and protein content in milk was assessed in an average milk sample once a month from each cow. The coefficient of variability (variation) was calculated according to the generally accepted formula.Results. As a result of the conducted research, it was found that the use of the “VivAktiv” feed additive did not significantly affect the productivity of cows. The milk yield for lactation by group was 10582.3 ± 187.35, 10640.8 ± 163.89, 10293.9 ± 176.45 and 10080.1 ± 178.13 kg, respectively, the difference was unreliable and insignificant. Considering the changes in the coefficient of variation between the groups, we can note a lower variability of the trait in cows of the 2nd experimental group. The animals of the control group came in second place. The largest variety of the trait turned out to be in the 3rd experimental group. Thus, the variability of the trait is influenced by the period (month) of lactation, to some extent the duration of lactation, as well as the use of the feed additive “VivAktiv”, which had a stabilizing effect on cow yield when used at a dose of 20 g/head/day.

A comprehensive review on anaerobic digestion with focus on potential feedstocks, limitations associated and recent advances for biogas production.

With the escalating energy demand to accommodate the growing population and its needs along with the responsibility to mitigate climate change and its consequences, anaerobic digestion (AD) has become the potential approach to sustainably fulfil our demands and tackle environmental issues. Notably, a lot of attention has been drawn in recent years towards the production of biogas around the world in waste-to-energy perspective. Nevertheless, the progress of AD is hindered by several factors such as operating parameters, designing and the performance of AD reactors. Furthermore, the full potential of this approach is not fully realised yet due the dependence on people's acceptance and government policies. This article focuses on the different types of feedstocks and their biogas production potential. The feedstock selection is the basic and most important step for accessing the biogas yield. Furthermore, different stages of the AD process, design and the configuration of the biogas digester/reactors have been discussed to get better insight into process. The important aspect to talk about this process is its limitations associated which have been focused upon in detail. Biogas is considered to attain the sustainable development goals (SDG) proposed by United Nations. Therefore, the huge focus should be drawn towards its improvements to counter the limitation and makes it available to all the rural communities in developing countries and set-up the pilot scale AD plants in both developing and developed countries. In this regard, this article talks about the improvements and futures perspective related to the AD process and biogas enhancement.

Screening for alkaliphilic microalgae for carbon capture from ambient air and food production

SummaryThe production of alkaliphilic microalgae can contribute to address the challenging cost of using pure carbon dioxide in large reactors. At high pH values, carbon dioxide is rapidly scavenged and the supply rates of dissolved inorganic carbon from the atmosphere to alkaline media are high. The present study aimed to identify microalgal strains that can cope with high alkalinity and aeration rates. Eight strains were studied and the microalgae Chlorella vulgaris, Scenedesmus almeriensis and Nannochloropsis gaditana were the only ones that were able to grow under these conditions. Their biomass productivities using laboratory‐scale bubble columns with no pH control and high aeration flow were 0.20 ± 0.03, 0.24 ± 0.03 and 0.08 ± 0.01 g·L−1·day−1, respectively. The production of the two former was scaled up to pilot‐scale bubble columns. Overall, the amount of atmospheric carbon dioxide that was transformed into biomass was in the range of 10%–30%, depending on the strain used and the photobioreactor setup. The biomass was rich in proteins and β‐carotene, both valuable products, highlighting the potential production of food ingredients while capturing carbon dioxide from the atmosphere.

Exploring Nature's Pharmacy: A Comprehensive Review of Natural Products as Potential Sources of Therapeutic Agents

Natural products have been integral to discovering numerous drugs with diverse pharmacological activities. This review provides a comprehensive analysis of the chemical diversity of natural products, their role in drug discovery, and their potential applications in modern medicine. Through critically evaluating recent research findings and case studies, we highlight the significance of natural products as sources of new drugs and discuss emerging trends and challenges in the field. Furthermore, we delve into the intricate interplay between natural products and drug discovery processes, elucidating their indispensable role in shaping therapeutic landscapes. We unveil the transformative potential of natural products as catalysts for novel drug development through a meticulous analysis of recent advancements and case studies. Additionally, we navigate the evolving trends and challenges confronting the integration of natural products into modern pharmacotherapy, offering insights into strategies for overcoming barriers and maximizing their therapeutic impact. In essence, this review serves as a comprehensive roadmap for understanding the multifaceted role of natural products in drug discovery and their profound implications for advancing modern medicine. Through a synthesis of empirical evidence and critical analysis, we aim to inspire further exploration and innovation in harnessing the untapped potential of natural products for addressing unmet medical needs and improving patient outcomes.

A new concept in assessing adaptability index for superior potential cropping intensity in early-maturing rice

Implementing a cropping intensity program with rice cultivation four times a year (CI 400) can be achieved using early maturing varieties of rice. However, this development needs to pay attention to the adaptability of the varieties planted to ensure successful implementation. The adaptability approach is a combination of assessing stability and productivity potential. This concept has been developed and applied in several studies, including research on rice. However, this approach is considered less comprehensive because it is non-parametric and only focuses on one stability analysis. Therefore, a systematic integration of various stability analyses, including index methods, is needed to comprehensively assess adaptability, particularly for early-maturing rice in South Sulawesi. This region is characterized by a dynamic climate zone and is one of the top four highest rice producers in Indonesia. Meanwhile, this study aims to develop a comprehensive adaptability index and select the best early-maturing rice varieties, especially in South Sulawesi. The investigation was conducted in Bone, Soppeng, and Gowa over two seasons using a nested randomized complete block design, with organized replications in each environment (location-season). Additionally, there was a significant focus on the application of five early-maturing and two check rice varieties, with each factor repeated three times at each location, totaling 126 experimental units. The results showed that the adaptability index, by combining stability rank accumulation with yield min max standardization, was effective at assessing the yield potential and stability of early-maturing rice varieties in supporting CI 400. Inpari 13 had the best index value at 0.55, followed by Cakrabuana at 0.31; hence both were recommended as adaptive early-maturing rice varieties, especially in South Sulawesi.

СТРАТЕГИЧЕСКИЕ ПРИОРИТЕТЫ И ПЕРСПЕКТИВНЫЕ НАПРАВЛЕНИЯ РАЗВИТИЯ ЮЖНОГО АГРОПРОМЫШЛЕННОГО ТЕРРИТОРИАЛЬНОГО КЛАСТЕРА КРАСНОЯРСКОГО КРАЯ

The purpose of the study is to analyze the priority areas and prospects for the development of the agro-industrial territorial cluster of the Southern macro-district of the Krasnoyarsk Region to increase competitiveness and ensure sustainable development of the region’s agricultural sector. The study was conducted on materials from the Krasnoyarsk Region according to the Federal State Statistics Service, accounting and financial statements of enterprises participating in the cluster. Research used theoretical and practical methods, including the study of scientific literature, statistical data analysis and strategic analysis methods. Based on the results of the study, it was established that, according to their production potential, the enterprises of the Southern Cluster can provide up to 65 % of the region’s needs for flour, up to 21 % for cereals, and up to 12 % for dairy products. The current reserves for growth in flour production volumes are 40 thousand tons, cereals – 5, mixed feed – 3, dairy products – 30–40 thousand tons, soft drinks – 1,7 million decaliters. To solve strategic development problems, cluster participants need to focus on such areas as the development of intra-cluster interaction and the formation of an effective organizational structure, the development of new products, penetration into new market segments, including exports, as well as the development of competencies for launching innovative projects. This process should be carried out through the implementation of joint projects on import substitution, the organization of joint logistics, as well as taking into account the development and implementation of innovative production technologies, the development of new products, expanding the number of cluster participants, training highly qualified personnel within the cluster, and lobbying for common interests at various sites.

Related or unrelated diversification: What is smart specialization?

In this paper, we investigate the nature of path dependency in specialization of countries in terms of international trade. Our focus is on the product space density metric, which is employed in the literature on smart specialization and the product space. We find that although density is supposed to capture relatedness between a country's current specialization pattern and potential products that it may diversify into, density also contains a large component of what we call unrelated diversity, i.e., country- and product fixed effects. Together, these fixed effects capture 97 % of the variance of density. We isolate the related variety component in density and then estimate logit regressions that predict gains or losses of specialization. The relative influence of unrelated variety on changes of specialization increases with the level of diversification of the country: only countries that are not already diversified are strongly influences of related variety. We also show that the impact of the country effect in the impact of unrelated diversity in gains or keeps of specializations increases with diversity. This suggests that existing capabilities breed new capabilities, i.e., diversification is a virtuous circle that is largely independent of relatedness, and that the source of path dependency in international specialization patterns lies much more in generic production capabilities rather than relatedness in product space.

Edge-Rich 3D Structuring of Metal Chalcogenide/Graphene with Vertical Nanosheets for Efficient Photocatalytic Hydrogen Production.

Constructing pertinent nanoarchitecture with abundant exposed active sites is a valid strategy for boosting photocatalytic hydrogen generation. However, the controllable approach of an ideal architecture comprising vertically standing transition metal chalcogenides (TMDs) nanosheets on a 3D graphene network remains challenging despite the potential for efficient photocatalytic hydrogen production. In this study, we fabricated edge-rich 3D structuring photocatalysts involving vertically grown TMDs nanosheets on a 3D porous graphene framework (referred to as 3D Gr). 2D TMDs (MoS2 and WS2)/3D Gr heterostructures were produced by location-specific photon-pen writing and metal-organic chemical vapor deposition for maximum edge site exposure enabling efficient photocatalytic reactivity. Vertically aligned 2D Mo(W)S2/3D Gr heterostructures exhibited distinctly boosted hydrogen production because of the 3D Gr caused by synergetic impacts associated with the large specific surface area and improved density of exposed active sites in vertically standing Mo(W)S2. The heterostructure involving graphene and TMDs corroborates an optimum charge transport pathway to rapidly separate the photogenerated electron-hole pairs, allowing more electrons to contribute to the photocatalytic hydrogen generation reaction. Consequently, the size-tailored heterostructure showed a superior hydrogen generation rate of 6.51 mmol g-1 h-1 for MoS2/3D graphene and 7.26 mmol g-1 h-1 for WS2/3D graphene, respectively, which were 3.59 and 3.76 times greater than that of MoS2 and WS2 samples. This study offers a promising path for the potential of 3D structuring of vertical TMDs/graphene heterostructure with edge-rich nanosheets for photocatalytic applications.

Combined selection for productivity, adaptability, and stability in corn genotypes for baby corn production

AbstractBaby corn is the name given to the female inflorescence of corn (Zea mays L.) before fertilization, being harvested in 2–3 days after the issuance of the styles‐stigmata. Currently, in Brazil, there is no specific corn cultivar for baby corn production, which leads to the use of popcorn and sweet corn cultivars because they are more accepted in the food industry. Therefore, the objective of this work was to estimate the genetic parameters and determine the efficiency of simultaneous selection based on productivity, adaptability, and stability of corn genotypes for baby corn production in the state of Espírito Santo, Brazil. The evaluations were conducted in 2019 on family properties in six municipalities in the state of Espírito Santo. Eleven corn genotypes were evaluated for baby corn production, being a double hybrid, four native varieties, and six commercial varieties. The design was randomized blocks with four repetitions each. The genetic parameters were estimated, which were obtained using the best linear unbiased prediction (BLUP), and the selection was carried out by the harmonic mean of the relative performance of genotypic values (HMRPGV). It was observed that the environments influenced the performance of the genotypes, configuring specific adaptation. The genotypes AG 1051, Aliança, and BR 106 presented the best performances in all the environments evaluated, considering, simultaneously, the productivity, adaptability, and stability of marketable ears. The HMRPGV method provided optimized selection of corn genotypes to produce baby corn with high productive potential, predictable behavior, and responsive to environmental stimuli.

Stimulation effect of network fracturing combined with sealing boundaries on the depressurization development of hydrate reservoir in China's offshore test site

This study investigated the stimulation of reservoir reconstruction by network fracturing combined with sealing boundaries (RC-HFBS) for hydrate development in China's offshore test site. We first studied the influence of different boundary-sealing characters on development performance. Then, the response of development performance to key production parameters including fracture conductivity (CF), fracture spacing (SF), and production pressure (Pp) under permeable and impermeable boundaries was studied. Lastly, the long-term commercial CH4 production potentials were evaluated. Results showed that compared to unreconstruction reservoirs, the CH4 production of RC-HFBS increased by 11.1 times, and the gas-to-water ratio increased by 13.3 times. The harm of permeable boundaries on reservoir depressurization leads to a short effective stimulation time of fractures (about 1000 out of 3650 days) and results in the insensitivity of exploitation performance to production parameters. However, the impermeable boundaries improve the fractures' stimulation, meanwhile, making exploitation performance extremely sensitive to production parameters. For RC-HFBS, commercial production can be realized by diverse production parameter combinations, including SF, CF, and Pp are 15 m, 10D•cm, 4 MPa; 15 m, 10D•cm, 2 MPa; 25 m, 10D•cm, 2 MPa; 5 m, 40D•cm, 4.5 MPa.