Potential Eh Research Articles

Nitrogen Inputs with Different Substrate Quality Modified pH, Eh, and N Dynamics of a Paddy Soil Incubated under Waterlogged Conditions

Synthetic fertilizer, livestock manure, and green manure are the typical nitrogen (N) sources in agriculture. This study was conducted to investigate the effects of different N sources on soil chemical environment and N dynamics. Changes in pH, redox potential (Eh), and concentration and δ15N of dissolved N [ammonium (NH4+), nitrate (NO3−), organic N, and total N] of soils treated with urea (U), pig manure compost (PMC), and hairy vetch (HV) were investigated in an incubation experiment under waterlogged conditions. The patterns of pH, Eh, and N concentration reflected both a greater mineralization potential of N derived from U than that from HV and PMC and easier decomposability of HV than PMC. The δ15N further suggested that nitrification was more active for U than for HV- and PMC-treated soils and that N loss via NH3 volatilization and denitrification would be greater for HV than U and PMC treatments.

Application of Exploratory and Spatial Data Analysis (EDA-SDA) to the Investigation of Metal Contamination in Groundwater from Electric Arc Furnace Slag and Dust

Electric arc furnace (EAF) dust and slag, materials which contain high metals in their composition, were improperly disposed in an industrial steel mill site between 1963 and 1999. Previous environmental investigations identified anomalous concentrations of metals in local groundwater but failed to relate these abnormalities to the disposed material or to natural geochemical processes. Aiming to identify the origin of such abnormalities, exploratory and spatial data analysis (EDA-SDA) method was applied on a hydrogeochemical data set obtained through 5 sampling campaigns in 32 groundwater monitoring wells installed upstream and downstream of the area impacted by the steel mill activities. Boxplot class-based and Eh vs. pH maps of physicochemical log-transformed data identified that wells located under the influence of EAF slag deposits in topographic hollows had lower Eh potential and increased electrical conductivity and pH, when compared to wells in the topographical nose of the surveyed area. Metal distribution maps showed that Al, Ca, K, Mg, Na, and Sr were consistently higher in topographic hollows while concentrations of Co, Cu, Cr, and Li were higher near the former steel-making plant, located in the topographical nose. Ba, Fe, Mn, and Zn, important indicators of EAF slag and dust, were observed in both topographic settings. Variable clustering was able to capture the relations among metals and thus validate the log-normalized data structure to be used into wells clustering. Clustering through the mclust algorithm carried out for two and three clusters allowed the distinction among localities that received an input of metals from dust or slag and those not influenced by either residue. This paper demonstrates that EDA-SDA is an effective method to identify areas under the influence of contamination from industrial activities from areas not affected by anthropogenic contamination.

Sediment Profile and Fluxes of Mercury and Methyl Mercury in Weihe Watershed in Henan, China.

Sediment core samples were collected from the Weihe River in February and August 2013. Cores were sectioned and analyzed for total Hg (THg) and methyl mercury (MeHg). THg in sediment cores ranged from 156 to 282 ng g(-1) in February, and from 172 to 300 ng g(-1) in August. MeHg concentrations ranged from 0.80 to 3.11 ng g(-1) in both seasons. Results showed that the diffusive fluxes were up to two orders of magnitude lower than the in situ benthic fluxes. Fluxes of MeHg measured in in situ experiments in August were positively correlated with [Formula: see text] (r = 0.930). THg and MeHg release were not strongly correlated with TOC in February. Patterns of MeHg flux differed greatly depending on oxidation-reduction potential (Eh) (r > 0.70) conditions in the two seasons.

S‐glutathionylation of Epithelial Sodium Channels in Neonatal Mouse Lung Regulates Alveolar Fluid Clearance in vivo

Hyperoxia causes oxidative stress and leads to a positive shift in the GSH/GSSG redox potential (Eh) in the lung. We hypothesize that an increase in the concentration of GSSG can post‐translationally modify lung epithelial sodium channels (ENaC) via S‐glutathionylation. We measured the Eh value of bronchoalveolar fluid (BAL) from postnatal day (PN) 10 C57Bl6 mice housed under room air (CTR; 21% O2) or hyperoxia (H85; 85% O2) and found that chronic H85 exposure elevates GSSG concentrations. In support of our hypothesis, single channel patch clamp analysis showed that GSSG (0.4 mM) decreases ENaC activity in alveolar type 2 cells (from 0.39 + 0.06 to 0.13 + 0.05). Real‐time X‐ray imaging illustrated that H85 and GSSG (0.4 mM) treatment significantly impairs the rate of lung fluid clearance compared to PN 10 controls; thus verifying the translational significance of single channel measurements. Predictive software analysis showed that a, b, and g‐ENaC subunits have conserved Cys thiols that can be directly S‐glutathionylated by GSSG. ENaC subunit pull‐down with biotinylated glutathione ethyl esters (BioGEE) confirms post‐translational modification of ENaC via S‐glutathionylation under hyperoxia‐induced oxidative stress. In conclusion, our data indicated that S‐glutathionylation of ENaC Cys thiols attenuates net salt and water reabsorption in the lungs. A better understanding of this novel regulatory pathway could lead to improved therapeutics for newborn infants under respiratory distress reliant on oxygen therapy.Acknowledgements: We acknowledge support from the APS Short‐Term Research Education Program to Increase Diversity in Health‐Related Research Fellowship.

Geochemical Analysis of Leached Elements from Fly Ash Stabilized Soils

AbstractLeaching controlling mechanisms of As, Ba, Ca, Cd, Mg, Se, and Sr from soil alone, fly ash alone, and soil-fly ash mixtures were investigated at a pH range of 1.5–14 through geochemical modeling. Results of the pH-static tests from previous studies indicated that As and Se concentrations increased at alkaline conditions, while Ca, Cd, Mg, and Sr concentrations increased when the pH of the aqueous solutions were acidic. Leaching of Ba tended to increase slightly at alkaline pH values. Effluent pH, oxidation-reduction (i.e., redox) potential Eh, and element concentrations measured from pH-static tests were used as input data in the geochemical model. Geochemical modeling results indicated that release of the elements, except As and Se, are controlled by the dissolution and precipitation of oxide, hydroxide, sulfate, and carbonate solids of the elements. For a given element, these solids controlling the leaching of elements were very consistent regardless of soil and fly ash types. Model-based predic...

Eletrochemical changes in Gleysol of the Amazon estuary

Electrochemical reactions are intensified by soil flooding, which changes the dynamics of nutrients and negatively affects plant growth. In this work, we aimed to evaluate the changes in the redox potential (Eh), pH and nutrient availability in a Haplic Gleysol soil from a floodplain of the Guama River, Belem, Para State. During the period of floods (61 days), samples soils were collected on alternate days and analyzed in dry and wet conditions. Flooding resulted in higher pH values and decreased Eh, which stabilized after 32 days of flooding and did not affect the values of total nitrogen. An increase in the concentrations of phosphorus, sulfur, iron, manganese, copper and zinc were observed, and they were subsequently reduced with time of submergence. The reduction of sulfur occurred at low Eh and pH values near neutrality. The results show that nitrogen and sulfur do not limit agricultural production in the lowland soils of the Guama River.

N-acetylcysteine as a potential strategy to attenuate the oxidative stress induced by uremic serum in the vascular system

AimsChronic kidney disease (CKD) progression is accompanied by systemic oxidative stress, which contributes to an increase in the risk of cardiovascular diseases (CVDs). N-acetylcysteine (NAC) is among the most studied antioxidants, but its therapeutic benefits in CKD-associated CVDs remain controversial. Here, we investigated whether NAC could inhibit the oxidative stress induced by uremia in vitro and in vivo. Main methodsEndothelial and smooth muscle cells were challenged with human uremic or non-uremic sera, and the effects of a pre-treatment with 2mM NAC were evaluated. Reactive oxygen species (ROS) production, protein oxidation and total glutathione/glutathione disulfide (tGSH/GSSG) ratios were measured. Five-sixths nephrectomized or sham-operated rats were orally treated (in the drinking water) with 60mg/kg/day NAC or not treated for 53days. Plasma cysteine/cystine reduction potential Eh(Cyss/2Cys) was determined as a novel marker of the systemic oxidative stress. Key findingsNAC inhibited all the determined oxidative stress parameters, likely by increasing the tGSH/GSSG ratio, in both cell lines exposed to uremic serum. Orally administered NAC attenuated the systemic oxidative stress in uremic rats. SignificanceThe present results indicate that NAC, by preventing GSH depletion in vascular cells exposed to uremic serum and by attenuating the systemic oxidative stress during CKD progression, emerges as a potential strategy to prevent the oxidative stress induced by uremic toxicity in the vascular system.

DNA Contents in Soil Contaminated with Heavy Metals

The study was performed to show how industrial activity affected soil quality in terms of soil DNA quality and quantity as well as soil characteristics. Soil material originated from an urban area of the Silesia Region (SW Poland). The soil characteristics were estimated: texture, moisture, pH, redox potential (Eh), and total carbon content (TOC), followed by determination of selected heavy metals (Pb, Cd, Zn, Cr, Fe, Cu). The last step was the isolation of soil DNA, its concentration and identification of microorganisms. The results showed that although the studied soil was heavily contaminated with heavy metals, there were still some metal-resistant microorganisms able to sustain soil activity. Moreover, these organisms are not present in the NCBI database, which encourages further studies aimed at identification of new organisms that may be useful in research of metal resistance as well as soil reclamation and remediation.

Sedimentology and water column stratification in a permanent anoxic Mediterranean lagoon environment, Aetoliko Lagoon, western Greece

The study area, Aetoliko Lagoon is a unique coastal Mediterranean landlocked ecosystem, controlled by tectonic activity and is characterized by permanent oxygen-depletion conditions (hypoxia/anoxia). The geological conditions, the geomorphology and the bathymetry of the lagoon, make the Aetoliko Lagoon an area of special interest for the research of the interplay between oxygen-depletion phenomena and human interference. Based on four research cruises within a time period of 1 year seasonal monitoring, in situ contemporary measurements in water column of physicochemical parameters pH, temperature (T, °C), salinity (S, ‰), dissolved oxygen (DO) and redox potential (Eh) were taken, and laboratory analyses of total organic (TOC) and inorganic carbon (IC), total nitrogen (TN) and total phosphorus were carried out. The water column is divided in three major layers: The upper layer (0–10-m depth) with seasonal salinities between 18.5 and 21.5 ‰; the middle layer (10–18.5-m depth) between 19.3 and 24.5 ‰; and the lower one (18.5–30 m) between 22.5 and 25.6 ‰. The lagoon’s lower layer was extended below 18-m depth with almost constant temperature of around 14–15 °C. Anoxic conditions were recorded below 10–11-m depth in July and November, and below 14-m depth in February and April. The vertical distributions of IC and TN values were strongly anticorrelated with the vertical distributions of DO and pH–Eh values as the result of physical, chemical and biochemical procedures. The spatial lagoon sediment characteristics such as grain size and moment measures indicate that the lagoon sediments consists mainly of poorly to very poorly sorted fine-to-coarse silt and sandy mud rich in TOC, while the geochemical parameters indicated a partition in the northern and southern parts of the lagoon. The surface sediments of the lagoon bottom act as a ‘reservoir’ for the diachronic reload of the water column with nutrients and support the assumption that the human interference plays a secondary role in the ecological status of the lagoon.

A note on domestic vs communal grain storage in the Early Helladic period

This paper sets out to propose an alternative model of economic management at settlements of Early Helladic I–II date, where evidence of socioeconomic hierarchies is not prominent in the archaeological material. It is suggested here that the remains of certain original structures within the boundaries of settlements were once granaries which served the whole community. If this reading of the material is accepted, then communal storage seems to have supplemented domestic storage or constituted the sole method of grain keeping at a number of settlements during the initial stages of the EH period. The practice was then abandoned and, with one exception, after the EH II–III break there is instead a strong case for domestic storage only. A potential EH I–II communal management of basic food supplies thus carries wider implications for the interpretation of the general management of settlements.

Generation of electric potential difference across the electrodes of the microbial fuel cell in the anaerobic oxidation of substrates by microbial associations

Several microbial associations were obtained from natural and anthropogenic sources. All of the associations grow well on glucose and significantly worse on acetate. We observed 80–95% glucose consumption during 3–5 days of growth. The oxidation of substrates by the cultures generates an electric potential difference between anode and cathode electrodes of a microbial fuel cell (MFC). The value of the potential difference depends on the nature of the association and the substrate and reaches 400–500 mV. The potential difference generation accompanied by a shift to the negative region of the medium redox potential (Eh) to — (400–500) mV. This indicates H2 evolution by the association cultures during oxidation of carbohydrates. Artificial redox mediators, such as tetramethyl-p-phenylenediamine, phenazine methosulfate, and benzyl viologen, were able to increase up to 15% the difference in electrical potential across the electrodes of the MFC. It is assumed that an increase in the potential difference across the electrodes induced by the redox mediators is due to their direct involvement in the transfer of electrons from the bacteria in the incubation medium to the MFC anode electrode. The direct measurement of current and potential difference on the electrodes in a short-circuit mode shows that the internal resistance of the MFC is equal to 1 kΩ and the power reaches 5 μW. Undoubtedly, this testifies to the low efficiency developed by the MFE.

Electrode measurements of the oxidation reduction potential in the Gotland Deep using a moored profiling instrumentation

The Gotland Deep Environmental Sampling Station (GODESS) operating between 30 m and 185 m was used to assess the variation of in situ redox potential (Eh) in the stratified water column of the central Baltic Sea, for a period of 56 days (November 2010–January 2011). The aim of this study was to acquire in-depth understanding of redox variations with the overall objective to identify the processes controlling Eh. At an interval of 8 h basic hydro-physical parameters were registered, including the oxidation-reduction potential, dissolved oxygen, chlorophyll a fluorescence, turbidity, temperature and conductivity. In total 170 profiles of all parameters were obtained. The measured Eh (with respect to standard hydrogen electrode, SHE) ranged from −0.055 V to 0.167 V. After temperature and pH correction of the standard reduction potentials, a comparison of the measured Eh with that calculated for the particular redox couples was carried out by applying the Nernst-equation. Furthermore, the concentrations of the most important redox elements such as Fe, Mn, N, O, C and S used for comparison were estimated by using empirical functions or were based on measurements of discrete water samples, taken at the time of deployment and recovery of the mooring. The obtained results reveal that the reduction of Fe(III) and O2 may be the main processes controlling the Eh potential in the Gotland basin. Below the redoxcline the reduction of hematite and then ferric oxyhydroxide could be related to the redox potential and somewhat deeper Fe(III)/Fe(II) was found to be the dominant redox couple. Although mixed potential theory could have been applied, the Fe couples appear to dominate over many other possible redox equilibria. This may be explained by the relatively high exchange current of Fe and by high Fe concentrations (up to 1.5 μM). Finally, a close correspondence to the Eh potentials measured in the oxic/hypoxic part of the water column was found considering the O2/O2−· and the O2−·/H2O2 couples. This suggests that one-electron transfer reactions determined the Eh under oxic/hypoxic conditions.

Release of Pharmaceuticals under Reducing Conditions in a Wastewater-Irrigated Mexican Soil

Wastewater irrigation is often performed by flood irrigation, leading to changes in redox potential (Eh) of irrigated soils. In addition to soil organic matter, Fe-(hydr)oxides are important sorbents for pollutants, and biotransformation of pollutants can be accelerated under reducing conditions. Here, the influence of reducing conditions on the release of sorbed pharmaceuticals from soil and their potential accelerated dissipation was investigated in a microcosm study. Samples of a soil from the Mezquital Valley (Mexico) irrigated for 85 yr with untreated wastewater were incubated under oxidizing (Eh of 500 ± 20 mV), weakly reducing (Eh of 100 ± 20 mV), and moderately reducing (Eh of -100 ± 20 mV) soil conditions for 30 to 31 d. The concentrations of nine pharmaceuticals (bezafibrate, carbamazepine, ciprofloxacin, sulfamethoxazole, trimethoprim, enrofloxacin, clarithromycin, diclofenac, and naproxen) were extracted via solid-phase extraction from soil slurries and analyzed by liquid chromatography-tandem mass spectrometry. Low Eh did not lead to a release of formerly sorbed pharmaceuticals from the wastewater irrigated soil. High pH values (>8) of the examined soil resulting from denitrification under reducing conditions prevented the dissolution of Fe-(hydr)oxides and, hence, the potential release of pharmaceuticals. A trend of decreasing concentrations of sulfamethoxazole and bezafibrate with time under moderately reducing conditions supports previous findings of a transformation of these compounds under anaerobic conditions.

Bioleaching of Covellite from Low Grade Copper Sulphide Ore and Tails

The economic importance of copper production in Chile has prompted the study of new strategies for the optimal management of mineral resources, including processing of low grade ore and even processing tails (processed ore). Currently, heap bioleaching is the most convenient technology to treat low grade copper sulphide ores bearing chalcopyrite, chalcocite and covellite. Covellite is a secondary copper sulphide occurring in copper sulphide deposits and it is also formed by acidic dissolution of chalcocite. In leaching processes, the main factors influencing the dissolution of covellite are the electrochemical potential (Eh) and temperature.This work aimed at assessing the effect of different concentrations of total iron (3.0 and 6.0 g/L) and temperatures (30 and 50°C) in the bioleaching of covellite contained in a low grade copper ore and the same ore after a previous process of bioleaching at ambient temperature. Initial composition of low-grade ore was chalcocite (0.58%), chalcopyrite (0.56%), covellite (0.30%) and pyrite (2.73%), while the processed ore contained chalcopyrite (0.47%), covellite (0.28%), chalcocite (0.10%) and pyrite (3.32%). Covellite was mostly dissolved (>90%) in both unprocessed and processed ore, independently of the temperature and total iron concentrations. However a higher total copper recovery was achieved in tests performed at 50°C due to higher dissolution of primary ores, especially in unprocessed ore. The microbial activity was confirmed by Most Probable Number (MPN) analyses of iron and sulfur oxidizing microorganisms. The microbial population was dominated by Acidithiobacillus spp. in tests operated at 30°C, while in tests operated at 50° Sulfobacillus spp. were the most abundant organisms.

Biologically Assisted Copper Secondary Sulfide Ore Leaching in the Presence of Chloride

Mining operations today face a difficult challenge in terms of water availability, with most of these deposits located in arid areas where fresh water is scarce. This is especially relevant for bio-mining operations where not only the source but the water quality can be a decisive factor for its successful utilization. This research evaluates the metallurgical and microbiological performance of an adapted chloride and copper-resistant mixed culture during the leaching (laboratory scale columns) of a secondary copper sulfide at different chloride concentrations. Up to 6 g/l chloride all columns established Eh potentials over 750 mV with complete oxidation of ferrous iron in the PLS showing well established microbial activity. Copper recovery in solution, along with a high redox potential, was established faster in the mesophilic column but leveled off quickly over time. Copper extraction showed that the 6 g/l chloride column has the highest metallurgical performance (83% CuT) with 4 extra points of copper recovery over the 1.5 g/L and 3 g/l Cl- columns. These results are a good indication that comparable copper recovery rates –to the ones achieved using fresh water- can be reached in the presence of important chloride concentrations by way of using the use of a suitable and well adapted microbial population. The related process and chloride-resistant culture have been patented under the following ID N°: EP2313534/US0201095.

Relating the onset of reduction to degree of soil water saturation

Literature does not indicate the degree of water saturation at which reduction is expected to occur. This study therefore aimed to determine the degree of water saturation (S) at which reduction is initiated. Soil cores were packed to a bulk density of 1.6 Mg m−3 and individually saturated to 60%, 70%, 80% and 90%. A set of cores was analysed every 3.5 d for three months and then once a week for one month. The experiment was terminated after 121 d. Reduction was defined as a decrease in redox potential (Eh) followed by an increase in the soluble Fe2+ concentration. The Eh, pH, Fe2+ and Mn2+ were all significantly affected by degree of water saturation. It was concluded from a laboratory experiment that Fe3+ reduction was initiated at a soil oxidisability (pe) of between 6.04 and 7.02 and at degrees of water saturation between S0.72 and S0.78. These findings were corroborated with a field experiment done where all in situ soil water measurements in the Weatherley catchment indicated that all the water extracted was reduced and that water extraction was primarily possible at S > 0.69. These determinations are a first confirmation of the hypothesis that reduction sets in at 70% water saturation.

The state of reduced glutathione in patients with impaired glycemic states and coronary heart disease

Antioxidative mechanisms prevent human body from the damaging action of free radicals and reactive oxygen species. Glutathione and related to it enzyme systems detoxicate H2O2 and hyperoxide and from there, reduced glutathione is a potential marker of oxidative stress level. Purpose: To investigete glutathione of erythrocytes in patients with prediabetes (IGT & IFG) and type 2 diabetes and concomitant coronary heart disease (CHD). Matherial and methods: We examined 25 patients with T2D and concomitant CHD and atherosclerosis (group 1), 20 patients with prediabetes (IGT & IFG) newly diagnosed and the same co-morbidity (group 2) and 47 almost healthy person (group 3). Examinations included clinical laboratory evaluation, fasting glicose, HbA1c. Total glutathione (GSHt) and oxidized glutathione (GSSG) were measered by use of glutathione reductase reaction. Redox potential (Eh) of glutathione was evaluated by use of Nernst equation. Results: Concentrations of GSHt and GSH were greater in group 2 to compare with groups 1 and 3 (by 4,2 times, p=0,012 and by 3,1 times, p=0,005 respectively). Increase of concentration of GSSG in group 1 may change extracellular redox condition. Calculation of Eh revealed its change to oxidized condition in group 1 to compare with group 2 (19,0 mV greater) and group 3 (15,4 mV greater). Conclusion: Antioxidant defense in red-cells is overactivated during prediabetes (IGT & IFG) with concomitant CHD and atherosclerosis while in patients with T2D was noticed depletion of compensatory mechanisms with increased GSSG and Eh. Reduction in 2GSH/GSSG suggests that intracellular antioxidant is lost; herewith cells become sensitive to oxidative stress.

The Effects of Manganese (II) But Not Nickel (II) Ions on Enterococcus hirae Cell Growth, Redox Potential Decrease, and Proton-Coupled Membrane Transport

Enterococcus hirae grow well under anaerobic conditions by fermenting glucose, accompanied with the decrease of oxidation-reduction potential (Eh) from positive values to negative ones. It was shown that heavy metals-copper and iron ions-affect E. hirae growth and alter Eh and proton-potassium ions fluxes through the cell membrane. The aim of this study was to establish the effects of manganese (II) ions on bacterial growth within the concentration range of 0.01-1 mM and compare with nickel (II) ions' effect. The presence of Mn(2+) during E. hirae ATCC9790 growth had significant effects: The lag phase duration decreased while the specific growth rate was increased; decrease in E h was shifted. In contrast, no visible changes in bacterial growth and Eh were observed in the case of Ni(2+). The effects of these ions on proton-potassium ions fluxes through the cell membrane were estimated in the presence and absence of N,N'-dicyclohexylcarbodiimide (DCCD), inhibitor of the FoF1 ATPase. Stronger effect of Mn(2+) on H(+)-K(+) exchange was detected in the presence of DCCD that can be explained by a possible complex formation between these substances and its direct influence on membrane transport proteins.

The Human CD8β M-4 Isoform Dominant in Effector Memory T Cells Has Distinct Cytoplasmic Motifs That Confer Unique Properties

The CD8 co-receptor influences T cell recognition and responses in both anti-tumor and anti-viral immunity. During evolution in the ancestor of humans and chimpanzees, the CD8B gene acquired two additional exons. As a result, in humans, there are four CD8β splice variants (M1 to M4) that differ in their cytoplasmic tails. The M-1 isoform which is the equivalent of murine CD8β, is predominantly expressed in naïve T cells, whereas, the M-4 isoform is predominantly expressed in effector memory T cells. The characteristics of the M-4 isoform conferred by its unique 36 amino acid cytoplasmic tail are not known. In this study, we identified a dihydrophobic leucine-based receptor internalization motif in the cytoplasmic tail of M-4 that regulated its cell surface expression and downregulation after activation. Further the M-4 cytoplasmic tail was able to associate with ubiquitinated targets in 293T cells and mutations in the amino acids NPW, a potential EH domain binding site, either enhanced or inhibited the interaction. In addition, the M-4 tail was itself mono-ubiquitinated on a lysine residue in both 293T cells and a human T cell line. When peripheral blood human T cells expressed CD8αβ M-4, the frequency of MIP-1β secreting cells responding to antigen presenting cells was two-fold higher as compared to CD8αβ M-1 expressing T cells. Thus, the cytoplasmic tail of the CD8β M-4 isoform has unique characteristics, which likely contributed to its selective expression and function in human effector memory T cells.

Sorption of 125I−, 137Cs+, 85Sr2+ and 152,154Eu3+ during their transport in undisturbed vertical and horizontal soil cores under dynamic flow conditions

Transport and sorption of 125I−, 137Cs+, 85Sr2+ and 152,154Eu3+ in undisturbed soil cores have been studied under dynamic conditions. Radionuclides were dissolved in synthetic groundwater (SGW) of 0.015 mol/dm3 ionic strength, pH 8.4 and redox potential Eh = 145 mV. The soil samples were taken vertically and horizontally from the river bed at 5–100 cm depths, about 120 m southward of the Dukovany Low and Medium Level Radioactive Waste Repository. The respective water-soluble carriers in the 10−6 mol/dm3 concentration were added into the SGW prior to the experiments. The soil cores of 9 cm height and 3.8 cm in diameter were placed into columns made of 150 cm3 PE injection syringes of suitable dimensions. The SGW was introduced into columns from the Mariotte’s bottle through a glass manifolds to supply the soil columns by gravity flow at a constant flow-rate about 1.2 cm3/h. Into the water stream, radioactive nuclides were added individually in a form of a short pulse in 0.1 cm3 of demineralized water. In the case of 125I− transport, retardation and hydrodynamic dispersion coefficients were determined, using an integral form of a simple advection-dispersion equation. For each radionuclides, sorption and desorption data have been obtained, evaluated and the results were compared mutually. Residual distributions of the sorbed cationic radionuclides along the soil columns were also presented.