Potential Development Prospects Research Articles

The Application and Trends of Blended Teaching Models in Teaching Chinese as a Foreign Language

In the context of technological advancements, such as "Internet +," the integration of online and offline elements in the form of blended teaching has emerged as a focal point in the realm of teaching Chinese as a foreign language. This study employs a literature analysis approach to initially summarize the current application status of blended teaching in the teaching of Chinese as a foreign language and to analyze three primary issues currently prevalent in this context. Subsequently, recommendations for the implementation of blended teaching in the teaching of Chinese as a foreign language are proposed from the perspectives of instructional design models, implementation of teaching strategies, and evaluation of teaching effectiveness. Finally, the potential development prospects of blended teaching in the teaching of Chinese as a foreign language are discussed.

Gene-edited cells: novel allogeneic gene/cell therapy for epidermolysis bullosa.

Epidermolysis bullosa (EB) is a group of rare genetic skin fragility disorders, which are hereditary. These disorders are associated with mutations in at least 16 genes that encode components of the epidermal adhesion complex. Currently, there are no effective treatments for this disorder. All current treatment approaches focus on topical treatments to prevent complications and infections. In recent years, significant progress has been achieved in the treatment of the severe genetic skin blistering condition known as EB through preclinical and clinical advancements. Promising developments have emerged in the areas of protein and cell therapies, such as allogeneic stem cell transplantation; in addition, RNA-based therapies and gene therapy approaches have also become a reality. Stem cells obtained from embryonic or adult tissues, including the skin, are undifferentiated cells with the ability to generate, maintain, and replace fully developed cells and tissues. Recent advancements in preclinical and clinical research have significantly enhanced stem cell therapy, presenting a promising treatment option for various diseases that are not effectively addressed by current medical treatments. Different types of stem cells such as primarily hematopoietic and mesenchymal, obtained from the patient or from a donor, have been utilized to treat severe forms of diseases, each with some beneficial effects. In addition, extensive research has shown that gene transfer methods targeting allogeneic and autologous epidermal stem cells to replace or correct the defective gene are promising. These methods can regenerate and restore the adhesion of primary keratinocytes in EB patients. The long-term treatment of skin lesions in a small number of patients has shown promising results through the transplantation of skin grafts produced from gene-corrected autologous epidermal stem cells. This article attempts to summarize the current situation, potential development prospects, and some of the challenges related to the cell therapy approach for EB treatment.

Recent trends in extraction, purification, structural characterization, and biological activities evaluation of Perilla frutescens (L.) Britton polysaccharide.

Perilla frutescens (L.) Britton is an annual herb plant of the Perilla genus in the Labiatae family, which is commonly utilized as an edible and medicinal resource. Polysaccharides are among the major components and essential bioactive compounds of P. frutescens, which exhibit a multitude of biological activities, including antioxidant, antitumor, anti-fatigue, immunoregulation, hepatoprotective, anti-inflammatory, and lipid-lowering effects. As a natural carbohydrate, P. frutescens polysaccharide has the potential to be utilized in the development of drugs and functional materials. In this paper, we provide an overview of progress made on the extraction, purification, structural characterization, and bioactivity of polysaccharides from different parts of P. frutescens. The challenges and opportunities for research are discussed, along with the potential development prospects and future areas of focus in the study of P. frutescens polysaccharides.

Advanced Framework for Integrating Risks into an Organizational Setting

Enterprise risk management (ERM) is a systematic approach that strategically assesses risk management from a holistic standpoint, including the whole company or enterprise. The aforementioned approach is a top-down strategic methodology designed to detect, evaluate, and proactively address possible risks, threats, hazards, and other sources of damage that have the potential to impede an organization's operations and goals, or result in negative outcomes. ERM is a prominent framework that assists businesses in the identification, evaluation, and management of hazards at the enterprise level. Scholars identified many elements that serve as motivators for enterprises to participate in the process of ERM. These reasons include the likelihood of encountering financial hardship and the subsequent expenses, subpar profits performance, potential development prospects, and the autonomy of the board. The implementation of an effective risk management plan might potentially serve as a competitive advantage for organizations, facilitating their growth. This elucidates the extensive corpus of research devoted to ERM. This paper examines the fundamental connections between Enterprise Architecture and Risk Management and presents a proposed architectural framework for effectively incorporating risk considerations within the broader organizational context. This article presents a proposed strategy for attaining a comprehensive and shared perspective on hazards throughout an organization.

Advances in the tea plants phenotyping using hyperspectral imaging technology.

Rapid detection of plant phenotypic traits is crucial for plant breeding and cultivation. Traditional measurement methods are carried out by rich-experienced agronomists, which are time-consuming and labor-intensive. However, with the increasing demand for rapid and high-throughput testing in tea plants traits, digital breeding and smart cultivation of tea plants rely heavily on precise plant phenotypic trait measurement techniques, among which hyperspectral imaging (HSI) technology stands out for its ability to provide real-time and rich-information. In this paper, we provide a comprehensive overview of the principles of hyperspectral imaging technology, the processing methods of cubic data, and relevant algorithms in tea plant phenomics, reviewing the progress of applying hyperspectral imaging technology to obtain information on tea plant phenotypes, growth conditions, and quality indicators under environmental stress. Lastly, we discuss the challenges faced by HSI technology in the detection of tea plant phenotypic traits from different perspectives, propose possible solutions, and envision the potential development prospects of HSI technology in the digital breeding and smart cultivation of tea plants. This review aims to provide theoretical and technical support for the application of HSI technology in detecting tea plant phenotypic information, further promoting the trend of developing high quality and high yield tea leaves.

Review on High-Efficiency External Cavity Diode Laser for Pr3+Doped Fiber

Wide tunable range, narrow linewidth, and high resolution are the most demanded for industry, medical science, communication systems, optical coherence tomography, and spectroscopy analysis. External cavity diode laser (ECDL) has been the preferred scheme to realize for wide-tuning and narrow-linewidth characteristics. ECDL is advantageous in terms of low noise, high side-mode suppression ratio, high-temperature stability, simple structure, and low cost. Primarily, we will discuss different types of semiconductor lasers and different technique schemes for tuning. Then we will focus on the tunability of laser beams of semiconductor materials by using diffractive grating. Finally, the potential development prospects of wide tuning and high-output power diode lasers are presented and discussed. Keywords: Semiconductor laser, Tunable, Narrow linewidth, diffractive grating.

The “De−Re Polymerization” Strategy: From Nondegradable Pet to Degradable Peat

AbstractPolyethylene terephthalate (PET) is a widely used plastic, but its short service life and non‐degradable features make it a major source of white pollution. In this context, the efficient recycling and rational reuse of PET have become a global research priority. In this paper, a deep eutectic solvent（DES）is prepared using methylurea and zinc chloride to catalyze the efficient PET glycolysis, which produces a high purity product bis(2‐hydroxyethyl) terephthalate (BHET), in 83.75% of yield. The product is condensed with diethyl adipate to produce Poly (ethyleneadipate‐co‐terephthalate) (PEAT). The relationships between the structural, thermal, mechanical, and degradation properties of PEAT with different intrinsic viscosities and composition ratios are studied. The addition of benzene ring improves the thermal properties and tensile strength of PEAT, but also makes them stiffer and less degradable. An increase in intrinsic viscosity can improve the thermal and mechanical properties to some extent but has little effect on the degradation. In general, the PEAT produced by the above process has good thermal properties, controllable degradation properties, and better mechanical properties than Poly (butyleneadipate‐co‐terephthalate) (PBAT). This work achieves the conversion of waste PET to high value‐added PEAT and has potential development prospects in recycling of PET.

Circular RNAs in Prostate Cancer: Is it Time to Further Explore Liquid Biopsies?

Although diagnosis and treatment of prostate cancer (PCa) have evolved rapidly in recent years, clinically significant molecular biomarkers are still needed to lower the mortality. Circular RNAs (circRNAs) are a poorly characterized component of PCa transcriptome. Recently, since the development of deep RNA sequencing and novel bioinformatic pipelines, emerging evidence suggests circRNAs to have diverse functions in the development and progression of PCa. Thus, we attempt to summarize the current situation and potential development prospects about the role of circRNAs in PCa liquid biopsies. The role of circRNAs in PCa was summarized by searching the literature related to circRNAs in PubMed in recent years. Deregulation of circRNAs is associated with cell proliferation, apoptosis, cell invasion, migration, as well as metastasis in PCa. Because of the high stability and tissue specificity of circRNAs, with improved detection methodologies, circRNAs may be predictive biomarkers in liquid biopsies. From the perspective of recent research, with the development of high-throughput sequencing and novel bioinformatics tools, knowledge of circRNAs will be further expanded. Improved technologies will make personalized precision medicine less of a paper exercise. It is time to further explore circRNA in liquid biopsies.

Review on Analysis Methods Enabled by Hyperspectral Imaging for Cultural Relic Conservation

In this review, the conservation methods for various types of cultural relics enabled by hyperspectral imaging are summarized, and the hyperspectral cameras and techniques utilized in the process from data acquisition to analyzation are introduced. Hyperspectral imaging is characterized by non-contact detection, broadband, and high resolution, which are of great significance to the non-destructive investigation of cultural relics. However, owing to the wide variety of cultural relics, the utilized equipment and methods vary greatly in the investigations of the associated conservation. Previous studies generally select a single type of cultural relic for conservation. That is, seldom study has focused on the application of hyperspectral techniques to generalized conservation methods that are simultaneously suitable for different types of cultural relics. Hence, some widely used hyperspectral cameras and imaging systems are introduced first. Subsequently, according to the previous investigations, the methods used for image acquisition, image correction, and data dimensionality reduction in hyperspectral techniques are described. Thirdly, a summary of methods in cultural relic conservation based on hyperspectral techniques is presented, which involves pigments, grottoes and murals, and painting and calligraphy. Later, some challenges and potential development prospects in hyperspectral-based methods are discussed for future study. Finally, the conclusions are given.

High-Throughput Phytochemical Unscrambling of Flowers Originating from Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) P. K. Hsiao and Astragalus membranaceus (Fisch.) Bug. by Applying the Intagretive Plant Metabolomics Method Using UHPLC-Q-TOF-MS/MS.

Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) P. K. Hsiao (MO) and Astragalus membranaceus (Fisch.) Bug. (ME) are two primary sources of the Astragalus herb, also known as "Huangqi" in China, which is widely applied to treat hypertension, glomerulonephritis, ischemic heart disease, and diabetes mellitus. As two different sources of the Astragalus herb, the chemical profiles of MO and ME may be different. Previous studies showed abundant differences in chemical composition between MO and ME. Therefore, the by-products of MO and ME, such as Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) P. K. Hsiao flower (MOF) and Astragalus membranaceus (Fisch.) Bug. flower (MEF), may have different phytochemical profiles. In this paper, a metabolomics method combined with ultra-high-performance liquid chromatography and electrospray ionization/quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS/MS) was employed to analyze the components of MOF and MEF. Consequently, the results of principal component analysis (PCA) showed that MOF and MEF could be separated clearly. In total, 31 chemical markers differentiating MOF and MEF were successfully identified, including 22 flavonoids, 8 isoflavones and 1 benzopyran. Among them, the contents of 18 components, including Calycosin, Cyanidin-3-O-glucoside, Quercetin, Rutin, Kaempferol, Formononetin, Isomucronulatol and Prim-O-glucosylcimifugin in MEF, were significantly higher than in MOF. In turn, the contents of another 13 components, covering Biochanin A, Tectoridin, Isomucronulatol-7-O-glucoside, Liquiritin, Rhamnetin, etc., were lower in the MEF group than that in the MOF group. It is worth noting that flavonoids, especially flavonoid glycosides, were the primary active chemical ingredients in MOF and MEF. The 18 ingredients in MEF with a higher level carried out diverse activities, like anti-oxidant, anti-inflammatory, anti-bacterial and anti-tumor activities, which led us to speculate that MEF may have greater pharmacological effects and potential development prospects than MOF. The present results displayed that the contents of ingredients in the two different species of plants were radically different, and there was significant uniqueness to the components of MOF and MEF. Our study not only provides helpful chemical information for further quality assessment and active mechanism research of MOF and MEF but also offers scientific support for the resource utilization of MOF and MEF.

Recent advances and applications of peptide-agent conjugates for targeting tumor cells.

Cancer, being a complex disease, presents a major challenge for the scientific and medical communities. Peptide therapeutics have played a significant role in different medical practices, including cancer treatment. This review provides an overview of the current situation and potential development prospects of anticancer peptides (ACPs), with a particular focus on peptide vaccines and peptide-drug conjugates for cancer treatment. ACPs can be used directly as cytotoxic agents (molecularly targeted peptides) or can act as carriers (guiding missile) of chemotherapeutic agents and radionuclides by specifically targeting cancer cells. More than 60 natural and synthetic cationic peptides are approved in the USA and other major markets for the treatment of cancer and other diseases. Compared to traditional cancer treatments, peptides exhibit anticancer activity with high specificity and the ability to rapidly kill target cancer cells. ACP's target and kill cancer cells via different mechanisms, including membrane disruption, pore formation, induction of apoptosis, necrosis, autophagy, and regulation of the immune system. Modified peptides have been developed as carriers for drugs, vaccines, and peptide-drug conjugates, which have been evaluated in various phases of clinical trials for the treatment of different types of solid and leukemia cancer. This review highlights the potential of ACPs as a promising therapeutic option for cancer treatment, particularly through the use of peptide vaccines and peptide-drug conjugates. Despite the limitations of peptides, such as poor metabolic stability and low bioavailability, modified peptides show promise in addressing these challenges. Various mechanism of action of anticancer peptides. Modes of action against cancer cells including: inducing apoptosis by cytochrome c release, direct cell membrane lysis (necrosis), inhibiting angiogenesis, inducing autophagy-mediated cell death and immune cell regulation.

Evaluation of Exosomal miRNA as Potential Biomarkers in Cervical Cancer.

Different studies show that small non-coding RNAs, such as microRNAs (miRNAs) obtained from exosomes, are considered potential biomarkers in several types of cancer, including cervical cancer (CC). Therefore, the present study seeks to present an overview of the role of circulating exosomal miRNAs with the potential to act as biomarkers for the diagnosis and prognosis of CC and to analyze the presence of these miRNAs according to the stage of CC. For this purpose, a review was developed, with articles consulted from the electronic databases MEDLINE/PubMed, Scopus, and Web of Science published between 2015 and 2021. Seven articles were included after a selection of studies according to the eligibility criteria. In addition to the methods used for sample analysis, detection, and isolation of miRNAs in each article, clinical data were also extracted from the patients studied, such as the stage of cancer. After analyzing the network of the seven miRNAs, they were associated with the immune system, CC progression and staging, and cisplatin resistance. With the belief that studies on miRNAs in cervical cancer would have major clinical implications, in this review, we have attempted to summarize the current situation and potential development prospects.

Proposed Framework and Method for Integrating Risks into an Organizational Setting

Enterprise risk management (ERM) is a systematic approach that strategically assesses risk management from a holistic standpoint, including the whole company or enterprise. The aforementioned approach is a top-down strategic methodology designed to detect, evaluate, and proactively address possible risks, threats, hazards, and other sources of damage that have the potential to impede an organization's operations and goals, or result in negative outcomes. ERM is a prominent framework that assists businesses in the identification, evaluation, and management of hazards at the enterprise level. Scholars identified many elements that serve as motivators for enterprises to participate in the process of ERM. These reasons include the likelihood of encountering financial hardship and the subsequent expenses, subpar profits performance, potential development prospects, and the autonomy of the board. The implementation of an effective risk management plan might potentially serve as a competitive advantage for organizations, facilitating their growth. This elucidates the extensive corpus of research devoted to ERM. This paper examines the fundamental connections between Enterprise Architecture and Risk Management, and presents a proposed architectural framework for effectively incorporating risk considerations within the broader organizational context. This article presents a proposed strategy for attaining a comprehensive and shared perspective on hazards throughout an organization.

Research Progress of Horizontal Cavity Surface-Emitting Laser.

The horizontal cavity surface emitting laser (HCSEL) boasts excellent properties, including high power, high beam quality, and ease of packaging and integration. It fundamentally resolves the problem of the large divergence angle in traditional edge-emitting semiconductor lasers, making it a feasible scheme for realizing high-power, small-divergence-angle, and high-beam-quality semiconductor lasers. Here, we introduce the technical scheme and review the development status of HCSELs. Firstly, we thoroughly analyze the structure, working principles, and performance characteristics of HCSELs according to different structures, such as the structural characteristics and key technologies. Additionally, we describe their optical properties. Finally, we analyze and discuss potential development prospects and challenges for HCSELs.

Preparation, Physicochemical Properties, and Structural Characterization of Resistant Starch-Aspirin-Loaded Microspheres.

BACKGROUND Resistant starch is a novel type of dietary fiber that can be considered as a natural polymer carrier with potential development prospects in the field of oral colonic release preparations since it can be degraded by bacteria in the large intestine. MATERIAL AND METHODS In this study, oral resistant starch-drug-loaded microspheres were prepared by spraydrying, and the response surface method was used to optimize the process based on the encapsulation efficiency. RESULTS The optimal preparation process conditions for the resistant starch-aspirin-loaded microspheres were as follows: core material: wall material ratio of 1: 1.98, chitosan solution concentration of 1.98%, and spray drying air inlet temperature of 130.45°C resulted in a reliable entrapment efficiency of 68.96%. Infrared spectroscopy analysis indicated that the encapsulated aspirin-starch microspheres did not differ significantly from the original resistant starch material. The ultrastructure of the drug-loaded microspheres was evenly wrapped with the capsule core and appeared as smooth spheres. The combination of resistant starch, aspirin, and chitosan resulted in a cross-linking reaction that reduced the overall gelatinization temperature compared with the original starch material alone. The light transmittance of the drug-loaded microspheres was slightly higher than that of the original resistant starch, while digestibility was similar to that of the resistant starch, indicating that the release would exist in the environment of the large intestine. CONCLUSIONS This study provides pivotal insights into the development of resistant starch in the field of colonic release preparations.

Preparation and Cr (VI) Removal Performance of a New Positively Charged PVC Microfiltration Membrane

Poly (vinyl chloride co dimethyl aminoethyl methacrylate) (PVD) and polyvinyl chloride (PVC) were blended, and then the tertiary amine groups of PVD in the casting solution were quaternized in situ with methyl iodide (CH3I) at 60. A new strongly charged positive PVC/PVD (M1) microfiltration membrane was prepared by non solvent induced phase transformation (NIPS), and PVC (M0) microfiltration membrane was prepared and compared with it. The membrane performance parameters such as morphology, pore size, charge, hydrophilicity and pure water flux of M0 and M1 were characterized. The cationic surfactant Octadecyltrimethylammonium chloride (STAC) micelle enhanced filtration was used to remove trace Cr6+(Cr2O7 2-) from the solution. The effects of Cr6+ concentration, pH value and sodium chloride (NaCl) concentration on the interception performance were investigated. The results show that the rejection rate of Cr6+ removal by M1 membrane micelle enhancement is close to 100%. When the mass ratio of Cr6+/STAC is greater than 0.478, the rejection rate of Cr6+ removal by M1 decreases with the increase of Cr6+ concentration; PH value has little effect on the retention of Cr6+ by M1; High concentration of NaCl reduced the rejection rate of Cr6+ by M1. M1 microfiltration membrane has potential development prospects in the treatment of wastewater containing Cr6+.

An eco-friendly fluorometric assay for high-sensitive meloxicam quantitation in biological matrices.

Meloxicam (Mel), as a powerful and effective anti-inflammatory drug, is commonly employed for the treatment of various inflammatory diseases; however, the use of Mel at high doses or for extended periods could cause severe side effects in human visceral organs. Therefore, a simple, rapid, and reliable method is urgently needed to monitor Mel in biological samples. Herein, novel water-soluble luminescent nano-carbon dots (nano-Cdots) with outstanding physicochemical properties were prepared by a one-pot high-temperature hydrothermal process of ellagic acid and guanidine. The nano-Cdots were further used as an optical probe for the sensitive detection of Mel in serum samples through the cooperative mechanisms of the inner filter effect and photoelectron transfer. By employing this sensor, an excellent linear correlation was achieved between the relative luminescent intensity [(PL0 - PL)/PL0] and the concentration of Mel in the range of 0.1 to 200 μM, with a limit of detection of 34.68 nM (3σ/k). This sensor was effectively employed for the analysis of Mel in real serum samples, implying its potential development prospects for the advancement of drug analysis with carbon-based probes.

Engineered olfactory system for in vitro artificial nose

• Artificial nose drew generous attention in the field of bionic organs • Construction with sensing module, recognition system and functional structure are introduced • Recognition technology, substrate materials and microarray integration in artificial nose are prospected The engineered biomimetic sensors can not only realize the action of organs, but also combine functional materials as in vitro organs by simulating the response of biological organs to different environmental signals. Artificial nose is a concept proposed by imitating biological olfactory system, simulating olfactory nerve cells, olfactory bulb and olfactory cortex through different materials to realize olfactory function. The sensor array used to sense external gas stimulation can be analyzed based on different recognition principles through different original signals such as optics, electricity, electrochemistry and bioelectricity. Furthermore, combined with pattern recognition and microarray technology, artificial nose can be highly integrated with biocompatible and other important properties to achieve in vitro application. The design principle and necessary components of artificial nose are introduced in this paper including sensing structure, recognition system and functional module. At the same time, the potential development prospects of molecular recognition technology, polymer-based materials and microarray integration in artificial nose are prospected.

Intestinal Flora Affect Alzheimer's Disease by Regulating Endogenous Hormones.

Alzheimer's disease (AD) is a central nervous system disease that can lead to cognitive impairment and progressive memory loss. An increasing number of studies have shown that intestinal flora play a crucial role in regulating the brain-gut axis. Short-chain fatty acids are metabolites of intestinal flora that regulate hormone synthesis and play an essential role in microbial-intestinal-brain communication. An imbalance of intestinal flora can promote microglia to secrete proinflammatory factors, cause nerve inflammation, and then affect cognitive and learning ability. However, the mechanism is not clear. From this, we infer that endogenous hormones may be the medium for intestinal flora to affect the process of AD. This review of the relationships among AD, endogenous hormones, and intestinal flora expounds on the critical role of various hormones in the brain-gut axis. It discusses intervention measures aimed at intestinal flora to prevent or delay AD occurrence. Finally, the potential development prospects of fecal microbiota transplantation in treating AD are put forward, which provide potential ideas for future AD research.

Advances in wide-tuning and narrow-linewidth external-cavity diode lasers

The external-cavity diode laser is advantageous in terms of low noise, high side-mode suppression ratio, high temperature stability, simple structure, and low cost, which has been the preferred scheme to realize wide-tuning and narrow-linewidth characteristics. Thus, it has been widely used in optical communication, lidar, environmental monitoring, spectral analysis, optical coherence tomography, and other frontier fields. Herein, we introduce the technical scheme and review the development status of wide-tuning and narrow-linewidth external-cavity diode lasers in detail. Primarily, the structure, working principles, and performance characteristics of external-cavity diode lasers are deeply analyzed according to different structures. The structural characteristics, key technologies, optical performance and application fields of state-of-the-art studies in recent years are discussed. Finally, the challenges and potential development prospects of wide-tuning and narrow-linewidth external-cavity diode lasers are analyzed and discussed.