Pot Method Research Articles

Standardization of screening technique for root rot disease (Macrophomina phaseolina) of castor

Root rot caused by Macrophomina phaseolina is a major disease of castor in endemic areas and under water stress conditions. Sick pot method was found better artificial inoculation method for screening of castor genotypes than stem tape inoculation method and sorghum grown inoculum of M. phaseolina @ 35 g/kg soil standardized for sick pot method. In both inoculation methods, parental lines ICS-303, ICS-304, ICS-305, ICS-319, DPC-22, and IPC-46 recorded <10% root rot incidence, while ICS-298, ICS-312, and ICS-321 recorded <20% root rot incidence.

Visible light mediated metal free photocatalytic synthesis of 2,4- disubstituted quinoline

A visible light-induced, photoredox catalyzed, eco-friendly one pot method for the synthesis of 2,4-disubsitituted quinoline has been proposed. This novel strategy utilizes visible light to produce 2,4-disubstituted quinoline through tandem reactions involving the formation of C-N and C-C bonds with high yield of product under relatively mild reaction conditions. This synthetic protocol has many important benefits, including a cost-effective catalyst, short reaction time, excellent atom economy, simple work-up and an extensive substrate scope.

Importance of Toxin Genes and Polymerase Chain Reaction-Based Open Reading Frame Type Analyses for Severe Staphylococcus aureus Infection in Children.

This study analyzed 26 Staphylococcus aureus strains, including 16 methicillin-resistant S. aureus (MRSA) and 10 methicillin-susceptible S. aureus (MSSA), collected from eight medical institutions in the Chiba Prefecture that requested a toxin gene analysis between 2015 and 2021. A total of 14 Panton-Valentine leukocidin (PVL) positive strains were identified, including MSSA. PVL-positive strains were classified into seven types according to polymerase chain reaction-based open reading frame typing (POT); of these types, three POT MRSA strains have not been previously reported, and one has been previously reported as PVL-negative. Some strains tested positive for both PVL and toxic shock syndrome toxin 1. One POT type was identified in both PVL-positive and PVL-negative strains. To the best of our knowledge, this is the first report on the regional spread of highly pathogenic S. aureus strains based on the POT method in children from multiple medical institutions. This method is useful for estimating the spread of toxin gene-carrying strains in the community owing to its association with toxin genes. As the number of PVL-positive strains in Japan increases, it is important to analyze the isolates of severe S. aureus infections in children by combining toxin gene analyses with the POT method.

Effect of Vermiwash and Vermicompost on the Growth of Fenugreek (Trigonella Sp.)

Vermicomposting is a useful and completely environmentally friendly technology that changes biodegradable waste into nutrient rich organic matter. This technology can also be used for obtaining vermiwash, nutrient rich bio liquid. It is a liquid extract, which is both useful and environmentally friendly. Vermicompost enriches the soil with nutrients and beneficial microorganisms, while vermiwash can provide a nutrient boost directly to the plant. The present study was done to assess the effect of different concentration of vermiwash and vermicompost on growth of Fenugreek through vermiponics and pot method respectively. The 25% vermiwash showed increase in shoot length as compared to control whereas 50% of vermiwash was found to be phytotoxic to the growth of Fenugreek plant as compared to control. Similar trend was observed in pot method using different concentrations of vermicompost. 25% vermicompost was found to increase the shoot length as compared to 50% and 75% vermicompost. Both vermicompost and vermiwash can be valuable addition to fenugreek cultivation. It is essential to use these products (vermiwash and vermicompost) in moderation i.e. 25% for maximizing the growth during cultivation.

Keparahan Penyakit Tungro dan Preferensi Wereng Hijau terhadap Berbagai Dosis Pemupukan Nitrogen

The severity of disease symptoms is affected by triangular interactions between pathogens, hosts, and the environment. This study aims to describe the severity of tungro disease symptoms concerning the resistance of rice varieties and various fertilization rates. The research used the potting method in the Center for Rice Research, Sukamandi greenhouse. The experiment was designed using a Randomized Design, with 3 varieties: Ciherang (sensitive), IR 64 (green leafhopper resistance), and Inpari 36 Lanrang (virus resistance) as the main plot, and 4 levels of combination fertilization: (1) compound fertilizer + without urea; (2) compound fertilizer and urea 250 kg/ha; (3) compound fertilizer and urea 350 kg/ha; and (4) compound fertilizer and urea 500 kg/ha, as subplots. The data was processed using Anova and Duncan's multiple distance test. The results showed that rice variety significantly affected vector preference and the severity of tungro symptoms. The dose of nitrogen fertilization does not give different results to the preferences of insects and the severity of tungro in the greenhouse. There is no interaction between the variety and the dose of fertilizing. Resistant varieties are less preferred by green leafhoppers than the sensitive varieties.
 
 Keywords: antixenosis, Nephotettix virescens nitrogen fertilizer, preference

Synthesis of five polyoxometalate-based compounds by using hydrothermal one pot method: Structures, supercapacitor, electrocatalytic and photocatalytic properties

Five polyoxometalate (POM) based compounds were synthesized by traditional hydrothermal method, namely {Co2(HTPT)2(H2O)2[Mo8O27]} (1), {Co2(TPT)2(H2O)6}(β-Mo8O26)·4H2O (2), {Co2(TPT)2(H2O)2(δ-Mo8O26)}·2H2O (3), {M(TPT)2(H2O)2}(Mo6O19) (M = Co 4, Ni 5) (TPT = 3-(4-terahydro-2H-pyran-4-yl)methyl)-4H-1,2,4-triazol-3-yl)pyridine). Compounds 1−3 were obtained in one pot, realizing efficient utilization of the hydrothermal method. The photocatalytic activities of compounds 1, 2, 4 and 5 were evaluated by the degradation of Methylene blue (MB) and Rhodamine B (RhB) and the reduction of Cr6+. The effects of the amount of catalyst and the pH of the system on the photocatalytic performance were also studied. Experimental studies have shown that compounds 1 and 4 also exhibit good bifunctional electrocatalytic activity for reducing NO2−, Cr6+ and H2O2, but also oxidizing AA (Ascorbic Acid). Compounds 1, 2, 4 and 5 can serve as amperometric sensors for NO2− and Cr6+. Compounds 1 and 4 own properties of capacitor materia.

Quercetin capped silver nanoparticles as an electrochemical sensor for ultrasensitive detection of chloramphenicol in food and water samples

This study demonstrates the green, rapid and simple synthesis of silver nanoparticles (AgNPs) by employing bio-flavonoid (quercetin), which acts as a stabilizing and reducing agent. The nanoparticles were prepared by a simple one pot method. AgNPs were characterized by using various characterization techniques like High-resolution transmission electron microscopy (HRTEM), Ultraviolet-visible spectroscopy (UV-visible), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR) and Field emission scanning electron microscopy (FESEM) with energy dispersive spectrometry (EDS). Later on, these nanoparticles were further used to construct the electrochemical sensing electrode and also for the precise and sensitive determination of chloramphenicol (CAP) antibiotic using cyclic voltammetry (CV) and linear sweep voltammetry (LSV). The fabricated sensor exhibits outstanding electrochemical performance, with a linear response for CAP concentrations between 0 μM to 80 μM and a limit of detection (LOD) of 265.6 nM. Further, the present sensor displayed impressive selectivity, reproducibility and high sensitivity. The designed sensor was deployed for the detection of CAP in real samples (water, milk and honey) to demonstrate its ability to perform real-time analysis with a satisfactory recovery rate. The results of real sample analysis using LSV were compared with the standard method (HPLC) and found comparable. Hence, the developed electrochemical sensor displayed great potential for detecting CAP in real samples with desirable recovery rates.

One-step preparation of anti-ferromagnetic single-atom nickel to improve oxygen evolution reaction of photoelectrochemical water splitting

Noble metal single-atom catalysts (SACs) have been extensively investigated due to their unique electronic structure and high-efficiency catalytic performance. However, there are relatively few studies on the preparation of non-noble metal single atoms and their application in oxygen evolution reaction (OER). In this paper, nickel single-atom catalyst was prepared using a facile one pot method. Ni-SAC-2.5% showed uniform SACs distribution and anti-ferromagnetic property as evidenced by the aberration-corrected high resolution electron microscopy and the first-principle calculations, achieving the lowest overpotential of 220 mV (10 mA/cm2) and stable performance in 1 M KOH solution. Meanwhile, it exhibited the largest specific capacitance value of 1.77 mF/cm2 and the lowest charge transfer resistance value of 40.75 Ω due to the accelerated kinetic process. First-principle calculations further confirmed the lowest computed overpotential of 0.54 eV for Ni-SAC-2.5%. However, Ni small clusters and nanoclusters with superparamagnetic performance were observed when the Ni content was further increased to 3.5% and 4.5%, resulting in higher overpotentials of 0.85 eV and 2.01 eV for OER process as indicated by the theoretical simulation. This paper provides a facile method to prepare non-noble metal SACs and useful guidance for designing high performance OER catalysts.

One-pot biogenic synthesis of gold nanoparticles@saponins niosomes: Sustainable nanomedicine for antibacterial, anti-inflammatory and anticancer therapeutics

Nanomedicine offers the promise of transforming modern medicine by facilitating precise and targeted therapeutic outcomes while reducing the adverse effects associated with conventional therapies. Nevertheless, the production process of nanomedicine often involves the use of hazardous and costly chemicals, leading to the creation of toxic by-products. This research paper presents a newly-developed, eco-friendly, one pot method of gold nanoparticles (AuNPs)@saponins niosomes synthesis by utilizing Sapindus mukorossi pericarps extract as both a reducing agent and stabilizer. The properties of the resulting AuNPs@saponins niosomes were analyzed in terms of composition, chemical structure, and morphology. Antibacterial activity of AuNPs@saponins niosomes was observed at concentrations of 50–100 µg/mL, and showed significant antibacterial effect on Staphylococcus aureus. The synthesized AuNPs@saponins niosomes also demonstrated potential as an anti-inflammatory agent, exhibiting an IC50 of 30.08 µg/mL, and displayed a dose-dependent anticancer effect against various human cancer cell lines up to 100 µg/mL. Among the tested cell lines, AuNPs@saponins niosomes exhibited the most effective cytotoxicity on MCF-7 cancer cells, displaying an IC50 of 54.13 µg/mL. Our results suggest that, this research has the potential to uncover the opportunities in the development of environmentally sustainable one-pot synthesis methods of metallic nanoparticles loaded niosomes, with applications in various fields for antibacterial, anti-inflammatory and anticancer therapeutics, offering promising avenues for advancement.

Physiological and proteomic changes of Castanopsis fissa in response to drought stress

Castanopsis fissa is a native, broadleaf tree species in Guangdong with characteristics of barrenness and fast growth and is often used as a pioneer species for vegetation restoration with excellent ecological benefits. To explore the response of C.fissa to drought, this study investigated the drought tolerance mechanism of C.fissa using physiological and proteomic assessments. Using a potted continuous drought experimental method with normal water supply as a control, we measured photosynthetic parameters, antioxidant enzyme activities, and osmoregulatory substances of C. fissa in response to drought stress for 1 to 4 weeks, respectively. In addition, we used TMT quantitative proteomics to identify differentially expressed proteins (DEPs) between the drought-stress-treated C. fissa leaves and the control leaves. With the extension of drought stress time, the photosynthetic indexes and peroxidase (POD) activity of C. fissa leaves showed a decreasing trend. The malondialdehyde (MDA) content; superoxide Dismutase (SOD) and catalase (CAT) activities; and proline (Pro), soluble sugar (SS) and soluble protein (SP) contents showed an overall increasing trend, all of which reached significant differences at 4 w of stress. We identified 177 and 529 DEPs in the 2 and 4 weeks drought-stress leaves, respectively, in reference to the control leaves. These DEPs were closely related to physiological metabolic processes such as photosynthesis, energy and carbohydrate metabolism, stress response and defense, transcriptional regulation, and signal ion transduction. Drought stress mainly affects photosynthesis, carbohydrate metabolism, and protein synthesis and degradation in C. fissa leaves. At 2 weeks of stress, the expression of carbon metabolism, pyruvate metabolism and ribosome-related proteins was significantly changed, however, and at 4 weeks of stress, protein processing in the endoplasmic reticulum and spliceosome-related proteins were significantly increased in plant leaves. To alleviate the effect of water unavailability, the drought-stressed C.fissa leaves increased its oxidative protective enzyme system to eliminate excess reactive oxygen species (ROS) and also increased its Pro and SP contents to maintain the intracellular osmotic potential balance.

Vector Angular Continuity in the Fusion of Coseismic Deformations at Multiple Optical Correlation Scales.

As one of the common techniques for measuring coseismic deformations, optical image correlation techniques are capable of overcoming the drawbacks of inadequate coherence and phase blurring which can occur in radar interferometry, as well as the problem of low spatial resolution in radar pixel offset tracking. However, the scales of the correlation window in optical image correlation techniques typically influence the results; the conventional SAR POT method faces a fundamental trade-off between the accuracy of matching and the preservation of details in the correlation window size. This study regards coseismic deformation as a two-dimensional vector, and develops a new post-processing workflow called VACI-OIC to reduce the dependence of shift estimation on the size of the correlation window. This paper takes the coseismic deformations in both the east-west and north-south directions into account at the same time, treating them as vectors, while also considering the similarity of displacement between adjacent points on the surface. Herein, the angular continuity index of the coseismic deformation vector was proposed as a more reasonable constraint condition to fuse the deformation field results obtained by optical image correlation across different correlation window. Taking the earthquake of 2021 in Maduo, China, as the study area, the deformation with the highest spatial resolution in the violent surface rupture area was determined (which could not be provided by SAR data). Compared to the results of single-scale optical correlation, the presented results were more uniform (i.e., more consistent with published results). At the same time, the proposed index also detected the strip fracture zone of the earthquake with impressive clarity.

Exploring the cytotoxicity on human lung cancer cells and DNA binding stratagem of camptothecin functionalised silver nanoparticles through multi-spectroscopic, and calorimetric approach

The influence of nanoparticles inside the human body and their interactions with biological macromolecules need to be explored/studied prior to specific applications. The objective of this study is to find the potential of camptothecin functionalised silver nanoparticles (CMT-AgNPs) in biomedical applications. This article primarily investigates the binding stratagem of CMT-AgNPs with calf thymus DNA (ctDNA) through a series of spectroscopic and calorimetric methods and then analyses the anticancer activity and cytotoxicity of CMT-AgNPs. The nanoparticles were synthesized using a simple one pot method and characterized using UV–Visible, fourier transform infrared (FTIR) spectroscopy, X-ray diffraction and high-resolution transmission electron microscopy (HRTEM). The average size of CMT-AgNPs is 10 ± 2 nm. A group of experimental techniques such as UV–Visible spectrophotometry, fluorescence dye displacement assay, circular dichroism (CD) and viscosity analysis unravelled the typical groove binding mode of CMT-AgNPs with ctDNA. The CD measurement evidenced the minor conformational alterations of double helical structure of ctDNA in the presence of CMT-AgNPs. The information deduced from the isothermal titration calorimetry (ITC) experiment is that the binding was exothermic and spontaneous in nature. Moreover, all the thermodynamic binding parameters were extracted from the ITC data. The binding constants obtained from UV absorption experiments, fluorescence dye displacement studies and ITC were consistently in the order of 104 Mol−1. All these results validated the formation of CMT-AgNPs–ctDNA complex and the results unambiguously confirm the typical groove binding mode of CMT-AgNPs. An exhaustive in vitro MTT assay by CMT-AgNPs and CMT against A549, HT29, HeLa and L929 cell lines revealed the capability of CMT-AgNPs as a potential anticancer agent.

0D/2D Nb2O5/ZnIn2S4 heterojunctions with enhanced utilization of light and separation of photogenerated carrier for efficient visible light photocatalytic performance

0D/2D heterojunctions via the single-photon excitation can avoid the light shielding of non-active component to promote the photocatalytic activity. However, it is very difficult to prepare ultrasmall nanoparticles loaded on 2D nanosheets via one pot method. Heren, we fabricated a Nb2O5/ZnIn2S4 (Nb-ZIS) heterojunction via a simple solvothermal process assisted by the Na2CO3 and thioacetamide (TAA), where zero-dimensional (0D) ultra-small Nb2O5 particles (≈10 nm) are uniformly in-situ grown on two-dimensional (2D) ZnIn2S4 nanosheets (ZIS). Structural characterizations and density functional theory (DFT) calculation suggest that the 0D-on-2D interface can not only alleviate the blocking effect of light, promoting light absorption for photocatalytic process, but also enhances the separation of photon-generated carriers through the built-in electric field (BIEF). Encouragingly, the Nb-ZIS heterojunction shows significant enhancement for H2 evolution and degradation of lignin, where the hydrogen evolution rate reaches 5.4 mmol h−1 g−1 and the lignin degradation rate achieves 78.5%, surpassing the commercial block Nb2O5 supported on ZIS heterojunction (Nb-ZIS-C) (4.0 mmol h−1 g−1 for hydrogen evolution and 61.9% for degradation) and the 0.2Nb/ZIS without the addition of Na2CO3 (4.5 mmol h−1 g−1 for hydrogen evolution and 72.3% for degradation), respectively.

Double network hydrogel based on curdlan and flaxseed gum with photothermal antibacterial properties for accelerating infectious wound healing

The healing of infected wounds has always been a clinical challenge. With the increasing threat of drug resistance due to antibiotic overuse, it is imperative to improve antibacterial wound dressings. In this study, we designed a double network (DN) hydrogel via a “one pot method” with antibacterial activity, and natural polysaccharides with the potential to promote skin wound healing were used. That is, a DN hydrogel matrix was formed by the hydrogen bond crosslinking of curdlan and the covalent crosslinking of flaxseed gum under the action of borax. We added ε-polylysine (ε-PL) as a bactericide. Tannic acid/ferric ion (TA/Fe3+) complex was also introduced into the hydrogel network as a photothermal agent to induce photothermal antibacterial properties. The hydrogel had fast self-healing, tissue adhesion, mechanical stability, good cell compatibility and photothermal antibacterial activity. In vitro studies of hydrogel showed its ability to inhibit S. aureus and E. coli. In vivo experiments also demonstrated the significant healing effect of hydrogel when used to treat wounds infected by S. aureus by promoting collagen deposition and accelerating the formation of skin appendage. This work provides a new design for the preparation of safe antibacterial hydrogel wound dressings and demonstrates great potential for promoting wound healing of bacterial infections.

Instant Pot for antigen retrieval: a simple, safeand economical method for use in immunohistochemistry.

Here, the authors report a simple method to perform antigen retrieval using a commonly available commercial Instant Pot® for immunohistochemistry. It provides a validated alternative to previous antigen retrieval methods that employ water baths, microwave ovensor scientific-grade pressure cookers. The Instant Pot can be set to obtain a variety of desired temperatures and is straightforward to use, making it extremely amenable to optimization. The Instant Pot method is an easy, safeand inexpensive alternative means to perform immunohistochemistry on formalin-fixed paraffin-embedded sections. It has been validated using several different monoclonal antibodies including ones directed against cell surface or intracellular antigens. As a result, it should be useful for a variety of research labs as well as undergraduate laboratory courses.

FORMULATION AND EVALUATION OF KALYANAKA KSHARA: AN AYURVEDIC COMPOUND FORMULATION

Background: Ayurveda utilises different forms of herbs in therapeutics. Kshara is one such form. The material destroys or cleans the excessive/morbid doshas (Ksharanat Kshananat va Kshara). They are considered anushastra’s (para-surgical instruments). Kshara’s (alkaline substances) are more suitable and can be used where shastra chikitsa (surgery) is contraindicated. Kshara (alkaline substances) is the best drug for internal and external use. Considering the quick action, lesser dose, and more comprehensive action, an attempt is made to formulate and standardise the product, as there is insufficient data regarding its standardisation. Objective: To formulate and standardise the Kalyanaka Kshara. Methods: All ingredients were taken per the reference Ashtanga Hridaya chikitsa sthana, Kalyanaka Kshara. All the Raw materials like plants, animals, and mineral drugs were collected from the local market of Jhansi, Uttar Pradesh. And by the sharava samputa (earthen pot) method, three different samples (KK1, KK2, and KK3) of Kalyanaka Kshara were prepared. Result: Physicochemical changes in each sample, organoleptic characters of the product yield were observed. The standard values of Kalyanaka Kshara are alkaline with pH 10.01, loss on drying (% w/w) 0.47, total ash value (% w/w) 55.5, acid insoluble ash value (% w/w) 0.93 water extractive value (% w/w) 62.55, alcohol extractive value (% w/w) 38.54. Conclusion: On behalf of each sample's pharmaceutical and physicochemical analysis, there are no considerable changes in any of the KK1, KK2, and KK3 samples, and encouraging results were observed. The developed SOP and physicochemical parameters can be adopted as pharmacopeial standards.

“Mica/silicate glass frit‐armored skeleton” in PDMS composite foam for improving fire‐proofing performance

AbstractIt remains challenging to give polydimethylsiloxane (PDMS) foam high fire‐proofing performance through scalable strategies and economical methods. Herein, a novel PDMS composite foam with good flame retardancy and simultaneously excellent thermal insulation performance and fire‐proofing properties under prolonged flame attack was developed through constructing “mica/silicate glass frit (SGF)‐armored skeleton” by one pot method. The prepared composite foams can resist above 1000°C flame without disintegration and still maintain excellent thermal insulation properties due to the formation of porous ceramic framework. The tensile strength and compressive strength at strain of 25% were also increased significantly by 265% and 221%, respectively. This strategy of constructing an inorganic filler‐armored skeleton structure in PDMS composite foam opens up new prospects to fabricate fire‐proofing PDMS composite foam with impressive high temperature resistance and good mechanical properties.

Tuning N/O-doped carbon materials for supercapacitors by direct pyrolysis of imidazolinium polymer

Using structurally adjustable polymer to develop pseudocapacitive carbon materials with rich faradaic-active functionalities in the readily accessible skeleton for supercapacitors is important and still challenging. In this paper, using the “aromatic aldehydes ammonia condensation” idea and starting from the terephthalaldehyde and ammonium, highly pseudocapacitive active porous carbon materials were fabricated by simple “one pot method” and pyrolysis. The iodine-modified imidazolinium network polymer synthesized carbon at 700 °C (INP-I-700) shows a high specific surface area (606 m2 g−1) and pore volume of 0.30 cm3 g−1. Thanks to the hierarchically microporous, mesoporous and macroporous structure and diverse pseudocapacitive active N,O species, the ammonium iodide synthesized imidazolinium polymer derived INP-I-700 porous carbon material demonstrates a high specific capacitance of 199.1 F g−1 at 1 A g−1, along with superior cycling stability for symmetric supercapacitor configure with 99 % capacity retention after 10,000 cycles in 1 M Na2SO4 aqueous electrolyte. Furthermore, an attractive energy density of 27.7 Wh/kg at power density of 496.5 W/kg in Na2SO4 electrolyte in the voltage range of 0–2.0 V for the assembled symmetric system can be reached. The outcome of this paper can provide guidance for the construction of functionalized carbon-based materials with large specific surface area.

Antioxidant Activity and Photosynthesis Efficiency in Melissa officinalis Subjected to Heavy Metals Stress

The aim of this study was to assess influence of cadmium and zinc treatments on antioxidant activity combined with the photosynthesis efficiency in a popular herb lemon balm (Melissa officinalis L.). Plants were grown under greenhouse conditions by the pot method. The Mn, Cu, Cd, and Zn contents in soil and plants were measured by HR-CS FAAS. The activity of net photosynthesis, stomatal conductance, transpiration rate, intercellular CO2, and index of chlorophyll in leaves were determined for all investigated species. Reduction of the net photosynthesis was observed for cultivations subjected to either Zn or Cd treatments. Phenolic contents were determined by the chemical Folin-Ciocalteu method, while enhanced voltammetric analysis was applied to assess the antioxidant properties of plant extracts. Both of these approaches yielded similar results. Herbal extracts had exceptional antioxidant capacities and were good scavengers of free radicals and reactive oxygen species. Structural similarity of cadmium and zinc facilitated their mutual structural exchange and prompted substantial expansion of phenolics under the mixed Zn and Cd treatments.

A reversible and selective chromogenic thiazole tagged chemosensor for Hg2+in aqueous medium: Crystal structure, theoretical investigations and real sample analysis

A novel thiazole tagged Schiff base chemosensor, (E)-N'-((2-aminothiazol-5-yl)methylene)-2-hydroxybenzohydrazide (ATH) has been synthesized in excellent yield by a simple one pot method and characterized by single crystal X-ray diffraction and spectroscopic techniques. The sensing ability of ATH was determined for detection of Hg2+ ions in the presence of different metal ions in aqueous medium. It behaves as an extremely selective and sensitive chromogenic sensor for naked-eye detection of Hg2+ ions with a colour change from colourless to yellow due to ligand to metal charge transfer. Remarkably, the binding constant (Ka) and limit of detection (LOD) for ATHHg2+ complex were found as 1.75 × 104M −1 and 8.85 × 10−7 M, respectively. The Job's plot indicated that the stoichiometric ratio of ATH to Hg2+ is 1:1. The binding mechanism was established based on FT-IR, 1H NMR titration, mass spectrometry and density functional theory studies. The chemosensor exhibited an excellent colorimetric property in 7.0–12.0 pH range. The chemosensor was found to be reversible and reusable in the presence of CN− ions with logic gate behaviour. Further, the chemosensor was utilized to sense Hg2+ ions in tap and Ganga river water samples.