Objective: To propose the SRE, an equation that includes concentration, motility, morphology and final sperm percentage to determine and compare the efficiency of Sperm Preparation Techniques (SPT). Design: A theoretical and deductive analysis. Materials/Methods: None. Results: One important step in ART is the processing of sperm samples to separate motile progressive (A and B) and morphologically normal sperms from seminal plasma and other undesired components. The different SPT search for a final high motile progressive (MP) and morphologically normal (MN) sperm concentration. We propose to integrate these parameters in an equation, SRE, to produce a unique # to compare these results. The equation is: SRE = SRI x FSF, where SRI is the Sperm Recovering Index and FSF is the Final Sperm Fraction. Thus, the efficiency of a SPT is given for the final concentration of MP and MN sperms (SRI) and the % of these sperms in the final sample (FSF). Consider the terms showed in the table, thus, the SRI is given for [CfxVfx(%A+%B)fx%Nf]/[CixVpx(%A+%B)ix%Ni]. The result is a # between 0 and 1. 0 represents a SPT with no MP and MN sperms recovered in the final sample and 1 represent a final sample in which all MP and MN sperms are recovered. The FSF is determined as a relation between the final concentration of MP and MN sperms regarding to the final sperm concentration as [CfxVfx(%A+%B)fx%Nf]/[Cf xVf]. The result is a # between 0 and 1, where 1 means that the final sample is constituted 100% for MP and MN sperms, and 0 means that this final sample does not have any MP and MN sperm. Thus SRE = FSF x SRI represent the components for a complete sample evaluation after SPT. The maximum value for SRE is 1, which means that the SPT recovered the whole MP and MN sperms present in the initial sample and these sperms represent the whole final sperm population. The minimum value for SRE is 0, which means that the SPT recovers no MP or MN sperm. Exist a threshold value, the SRE-basal that is a minimum value expected after any processing technique. The SREb = SRIbxFSFb = {[Ci x Vi x (%A+%B) i x %Ni]/[Ci x Vi x (%A+%B) i x %Ni]} x {[Ci x Vi x (%A+%B)ix %Ni/Ci x Vi]}. The initial and final values for all terms of this equation are the same, because we expect to have after the SPT at least the same sperms found in the initial sample. Thus SRIb is 1 and FSFb acquires a value that is particular for each sample and depends of its initial parameters. Values of SRE below the SRIb means that the SPT made worse the sample respect to the initial one, in terms of MP and MN sperm concentration and/or final sperm proportion. Values above the SREb means an improvement respect to the initial values. There exists a Delta-SRE (ΔSRE) = SRE-SREb, which represents a variation regarding to the basal value and permits a quantification of post-processing result indicating how much the sample changed respect to the basal value. Tabled 1TermsDescriptionCiinitial sperm concentrationVpvolume of sample prepared(%A + %B)iinitial % of sperms A and B%Niinitial % of MN spermsCffinal sperm concentrationVffinal sperm volume(%A + %B)ffinal % of sperms A and B%Nffinal normal sperm % Open table in a new tab Conclusions: The SRE is a useful measure to quantify the sperm processing techniques results, which could make more feasible its interpretation and analysis, generating a better comprehension of the process entailed in the SPT and promoting the development of better technique and mediums with this purpose. Supported by: None.
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