Post Preparation Research Articles

Role of hydrogen in the photoinduced evolution of porous silicon luminescence

AbstractPhoto‐induced post preparation evolution effects in porous silicon were studied by IR, EPR, photoluminescence, and hydrogen effusion spectroscopies. The results show that two independent mechanisms are present during the photo‐induced evolution. We also show that hydrogen photo‐effusion takes place, in agreement with our previously proposed model. Photo‐effusion experiments performed in vacuum, combined with IR and photoluminescence spectroscopies allow to discriminate the competing mechanisms present in the evolution of the photoluminescence. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

One-day bowel preparation with sodium phosphate prior to colorectal surgery: a prospective, randomized, controlled clinical trial

To investigate the feasibility and safety of one-day bowel preparation for colorectal surgery. Forty patients undergone colorectal surgery were divided randomly into the Control group and the Experimental group and received 3-day magnesium sulfate and 1-day sodium phosphate bowel preparations before the operation, respectively. The levels of hemoglobin, hematocrit, serum electrolytes, and anaerobe counts in the stool prior and post bowel preparation were examined. The general status, surgical complications, and structure of intestinal mucosa in the patients were observed after the operation. There was no significant difference in the anastomoses healing, infectious complications, serum tests and intestinal mucosa structures between the two groups. Less diarrhea occurred prior and post the surgery in the experimental group, and they felt better with the bowel preparation. The anaerobe counts in stool were higher after the bowel preparation than before in both groups. One-day bowel preparation with sodium phosphate is a safe and reliable method for colorectal surgery. The shortening of preparation time can reduce the degrees of uncomfortable feeling and disruptions of intestinal micro-ecology and barrier.

Impact of Endodontic Treatments on the Rigidity of the Root

The destabilizing effect of endodontic treatment upon teeth is still controversial. The purpose of this study was to investigate the effects of different steps of endodontic treatments upon the rigidity of teeth. Extracted untreated central maxillary anterior teeth were loaded (3.75 N), and deformations of the root were assessed by Speckle pattern interferometry. The following treatments (with subsequent determination of deformability) were conducted sequentially: access preparation, manual instrumentation (Kerr files ISO-40, ISO-60, ISO-80, ISO-110), and tapered and parallel-sided post preparation. It was found that the teeth were increasingly destabilized by any treatment. While the increased deformability was not significant with the manual enlargement (p > 0.05), we found a significant destabilization after access preparation and post preparation (p < 0.05). A corresponding difference was found after conversion of the post preparation from tapered to parallel-sided (p < 0.05). Both substance loss and modifications of the natural root canal geometry play an important role in tooth rigidity.

New Trends in Magnetic Exchange Bias

New Trends in Magnetic Exchange Bias

Quantitative Chemiluminescent Immunoassay for NF‐κB–DNA Binding Activity

Nuclear factor‐κB (NF‐κB) is a ubiquitous redox‐sensitive transcription factor involved in the pro‐inflammatory response to several factors, including cytokines and oxidative stress. Upon activation, NF‐κB translocates into the nucleus and binds to specific nucleotide sequences. The cellular responses to inflammatory and stress signals have been implicated in disease conditions, such as atherosclerosis, cancer, diabetes, and Alzheimer's disease. The conventional method for detection of NF‐κB ‐DNA binding activity is the electrophoretic mobility shift assay (EMSA), which is time‐consuming and non‐quantitative. Here, we report (a) development of a rapid, sensitive and quantitative chemiluminescent immunoassay (QCI) for analysis of NF‐κB DNA‐binding activity, and (b) validation of the QCI with the EMSA using nuclear and cytosolic extracts from cultured prostate cancer cells (PC3), rat liver homogenates and human lymphocytes. The QCI for analysis of NF‐κB DNA binding activity has advantages over the EMSA: (1) Higher speed: 3–5 h post sample preparation, (2) Greater sensitivity: 10 pg NF‐κB/well, (3) Quantitative: linear range: 10–1000 pg NF‐κB; r2 = 0.999 (4) High throughput adaptability: 96‐well plate format can analyze up to 40 samples in duplicate, (5) Safety: No radioactive isotopes, (6) Simplicity, and (7) Capability of measurement of both activated (free) NF‐κB which is translocated into the nucleus and total (bound + unbound) NF‐κB present in the cytosol/cell.

Solid Lipid Nanoparticles (SLNs): Preparation and Properties of Calix[4]resorcinarene Derived Systems.

Solid Lipid Nanoparticles (SLNs) have been prepared fromc-2,c-8,c-14,c-20-tetraundecyl-4,6,10,12,16,18,22,24-octahydroxyresorc[4]areneas colloidal suspensions. Photon Correlation Spectroscopy studies revealed aparticle hydrodynamic diameter of 150 nm. Non-contact mode Atomic ForceMicroscopy allows observation of the particles as slightly flattened sphericalobjects of 236 (±40) nm diameter and 145 (±40) nm height. Thestudy of the preparation parameters showed that shear force does not affectthe hydrodynamic size of the SLNs. In contrast, the viscosity and the pH ofthe aqueous phase, the amphiphile concentration in the organic phase and thevolume of organic phase used, all lead to variation in the size of the particles.In term of post preparation parameters only the ionic strength has been shownto affect significantly the particle size; while the pH of the storing solution,microwave, ultrasonic and thermal treatments do not. Short and long-termstability studies have been performed to measure the effect of the ionic strengthon the stability of the particles. The use of carbohydrate cryoprotectants does notallow re-dispersion of the colloidal suspension after freeze-drying.

Post Retention: The Effect of Sequence of Post-Space Preparation, Cementation Time, and Different Sealers

Many endodontic sealers contain constituents that have been shown to inhibit the polymerization of resin cements. This may be important when prefabricated posts are cemented at the same appointment as root canal obturation. This study evaluated the effects of cementing posts with a resin cement immediately or at a delayed time period after obturation using Roth's 801 Elite Grade or AH26 sealer cements. The contribution of mechanical post-space preparation was also assessed as a critical variable. One hundred twenty extracted canines were randomly divided into eight experimental groups. The variables evaluated were the order of post preparation (either before or after obturation), the type of sealer used, and the time of post cementation. All teeth received a stainless steel #6 Parapost XP cemented with a resin cement, Panavia 21. Each experimental group underwent tensile testing for retention using an Instron universal testing machine. For both sealers posts cemented in teeth in which the canal was obturated before post-space preparation and thus had sealer-contaminated dentin removed by the space preparation procedure had significantly higher retentive values than those obturated after post-space preparation in which contaminated dentin might remain. Sealer used and time of cementation had no specific effect on retention. Achieving a clean, "freshened" dentinal surface during mechanical post-space preparation seems to be a critical variable for post retention when a resin cement is used.

Radiographic Measurement of Residual Root Thickness in Premolars with Post Preparation

A minimum thickness of 1 mm has been suggested for the residual root wall in post preparation. The objective of this paper is to evaluate the reliability of radiography when used to measure root thickness. In 106 upper first premolars with one and two root canals, post preparations were made with Peeso reamers to a depth in the root equal to the crown length. Bucco-palatal radiographs were taken of each tooth. The films were processed and projected with a magnification of x20. The smallest thickness of the mesial and distal walls of each root at the apical end of the preparation was measured and recorded. The teeth were then cut at the level of the measurement, and the smallest thickness of the proximal walls was measured using a microscope with a micrometric eyepiece. Fourteen percent of the teeth with one root canal and 27% of the teeth with two root canals could not be measured due to blurred radiographic contours. Variance analysis showed a highly significant difference (p < 0.001) when radiographic and anatomical measurements were compared. The radiograph showed greater thicknesses than were actually present and should not therefore be considered to be a reliable method for measuring residual thickness of tooth walls after post preparation.

The size of occlusal rest seats prepared for removable partial dentures

Objective The aim of this study was to test whether rest seats cut by a group of general dental practitioners for a removable partial denture differed in size and shape from those prepared by either a group of postgraduate students or their academic teachers. Method The occlusal surfaces of a number of plastic teeth were scanned by a laser profilometer. Each tooth was then placed in a set of articulated phantom head dental arches. 30 dental practitioners, 16 postgraduates and 11 dental academics were asked to cut a rest seat preparation in the mesial marginal ridge suitable for the construction of a removable chromium cobalt partial denture. The tooth was removed from the models, rescanned, and this data converted to grey level images for measurement of the width, length and area of each rest seat. Depth was calculated as the difference between the pre and post preparation scanned profiles. Results There was a wide variation in the size of the individual rest seat preparations. There was no significant difference between the measured parameters from the images of the preparations made by academic staff and postgraduate students. The two sets of data were therefore combined. The length, width and area of the rest seats prepared by the staff and postgraduate group were significantly greater than those cut by the dental practitioner group. However, there was no significant difference in the depths measured. The outline form of the rests prepared by the dental practitioners was often round with sharply defined margins contrasting with the smooth triangular preparation the staff and postgraduates prepared. Conclusion A 'refresher' in tooth modification for GDPs designing partial dentures would improve the long term success of the prosthesis.

The size of occlusal rest seats prepared for removable partial dentures

The aim of this study was to test whether rest seats cut by a group of general dental practitioners for a removable partial denture differed in size and shape from those prepared by either a group of postgraduate students or their academic teachers. The occlusal surfaces of a number of plastic teeth were scanned by a laser profilometer. Each tooth was then placed in a set of articulated phantom head dental arches. 30 dental practitioners, 16 postgraduates and 11 dental academics were asked to cut a rest seat preparation in the mesial marginal ridge suitable for the construction of a removable chromium cobalt partial denture. The tooth was removed from the models, rescanned, and this data converted to grey level images for measurement of the width, length and area of each rest seat. Depth was calculated as the difference between the pre and post preparation scanned profiles. There was a wide variation in the size of the individual rest seat preparations. There was no significant difference between the measured parameters from the images of the preparations made by academic staff and postgraduate students. The two sets of data were therefore combined. The length, width and area of the rest seats prepared by the staff and postgraduate group were significantly greater than those cut by the dental practitioner group. However, there was no significant difference in the depths measured. The outline form of the rests prepared by the dental practitioners was often round with sharply defined margins contrasting with the smooth triangular preparation the staff and postgraduates prepared. A 'refresher' in tooth modification for GDPs designing partial dentures would improve the long term success of the prosthesis.

Who cuts the better occlusal rest seats? GDPs versus academics

Objective The aim of this study was to test whether rest seats cut by a group of general dental practitioners for a removable partial denture differed in size and shape from those prepared by either a group of postgraduate students or their academic teachers. Method The occlusal surfaces of a number of plastic teeth were scanned by a laser profilometer. Each tooth was then placed in a set of articulated phantom head dental arches. 30 dental practitioners, 16 postgraduates and 11 dental academics were asked to cut a rest seat preparation in the mesial marginal ridge suitable for the construction of a removable chromium cobalt partial denture. The tooth was removed from the models, rescanned, and this data converted to grey level images for measurement of the width, length and area of each rest seat. Depth was calculated as the difference between the pre and post preparation scanned profiles. Results There was a wide variation in the size of the individual rest seat preparations. There was no significant difference between the measured parameters from the images of the preparations made by academic staff and postgraduate students. The two sets of data were therefore combined. The length, width and area of the rest seats prepared by the staff and postgraduate group were significantly greater than those cut by the dental practitioner group. However, there was no significant difference in the depths measured. The outline form of the rests prepared by the dental practitioners was often round with sharply defined margins contrasting with the smooth triangular preparations of the staff and postgraduates. Conclusion A 'refresher' in tooth modification for GDPs designing partial dentures would improve the longterm success of the prosthesis.

Residual thickness of root in first maxillary premolars with post space preparation

It has been suggested that, to ensure tooth strength, a minimum of 1 mm of root wall thickness should be left after post preparations. The purpose of this study was to determine the instrument diameter that will not affect this measurement in maxillary first premolars. Post preparations were made in 106 teeth with one and two root canals at a working depth equal to the anatomical crown length, with 0.70, 0.90, 1.10, 1.30, 1.50, and 1.70 mm diameter instruments. Sections were cut perpendicular to the long axis of the tooth at the cervical and apical ends of each preparation, and the minimum width of residual root was measured on each wall, at both sites. A binocular microscope with a micrometer eyepiece was used. At the cervical level of the preparation, no group showed a wall thickness < 1 mm. Data for the apical sections was statistically analyzed, and the corresponding confidence limits were calculated with 95% confidence on the mean. The results show that the minimum residual thickness was only preserved when 0.70 mm instruments were used in single-canal roots and when 1.10 mm or smaller instruments were used for two-canal roots. This seemingly anomalous result occurs because fluting on both the mesial and distal sides of the root impinge on single canals, whereas dual canals are buccally or lingually displaced to an area of thicker root diameter.

Alteração da temperatura radicular externa durante o preparo para contenção intra-radicular

O objetivo deste trabalho foi comparar in vitro a alteração de temperatura na superfície radicular externa durante o preparo para contenção intra-radicular utilizando 4 técnicas de remoção da guta-percha: condensadores aquecidos, brocas de Peeso, Gates-Glidden e Largo, em 40 dentes pré-molares inferiores (unirradiculares) tratados endodonticamente. O experimento foi realizado em uma câmara com temperatura controlada em torno de 26°C. Para a remoção da guta-percha do interior dos canais, foram calculados dois terços do comprimento da raiz, deixando em torno de 4 mm de obturação apical. As medições da temperatura radicular externa foram realizadas com um termopar acoplado a um multímetro e efetuadas em 3 regiões: cervical (em torno de 3 mm abaixo da junção cemento-esmalte), média e apical. A análise de variância indicou diferenças significantes (p &lt; 0,01) entre as regiões e as técnicas utilizadas. O teste complementar de Tukey indicou que os condensadores aquecidos produziram o maior aumento de temperatura dentre as técnicas, e a broca de Peeso dentre as brocas, seguida pela de Largo e de Gates-Glidden. Dentre as regiões, a apical apresentou a maior alteração de temperatura em todas as técnicas avaliadas.

Rapid preparation method for technetium-99m bicisate.

The method currently recommended for the preparation of technetium-99m bicisate (99mTc-bicisate) requires a lengthy 30-min incubation at room temperature. The purpose of this study was to evaluate an alternative method to shorten the preparation time. 99mTc-bicisate was prepared with 3.7 GBq (100 mCi) 99mTc according to the manufacturer's instructions, except for the final incubation step, which was replaced with the microwave heating procedure. A standard thin-layer chromatography (TLC) method (i.e., Baker-Flex silica gel IB-F TLC plate with ethyl acetate as mobile phase) was used for the determination of the radiochemical purity (RCP) of 99mTc-bicisate. Our evaluation with different microwave heating processes (300 W with different heating times) demonstrated that as the microwave heating temperature was increased (i.e., 44 masculine-71 masculine C), an increased percentage of samples reached 95% within 5 min post preparation (n=58). The highest RCP value (i.e., 97.4%+/-0.5%, n=10) could be obtained immediately after an 8-s microwave heating time at 300 W (microwave temperature at 69 masculine C), and an average RCP value of 96.4%+/-1.3% (n=90) was maintained throughout the 24-h evaluation period. However, the trend seemed to reverse at higher microwave temperatures (i.e., 76 masculine-90 masculine C), which reconfirmed our initial findings that overheating had no benefit for the preparation of 99mTc-bicisate. To ensure that temperature was the only determining factor, a hot water incubator set at 69 masculine C was used (n=6). Similar RCP results were achieved. In conclusion, the use of a microwave oven at a low heat cycle provides a rapid and efficient way to prepare 99mTc-bicisate.

Root perforations: classification and treatment choices based on prognostic factors.

Root perforations are common complications of endodontic treatment or post preparation and often lead to tooth extraction. Successful treatment depends mainly on immediate sealing of the perforation and prevention of infection. Several factors affect the achievement of these goals, most important of which are: time of occurrence, size, and location of the perforation. A classification of root perforations, based on the above factors, is presented to assist the clinician in the choice of the treatment protocol which will give the best possible results when a perforation is diagnosed.

Effect of immediate and delayed post preparation on apical dye leakage using two different sealers

Eighty freshly extracted teeth were endodontically prepared and filled with gutta-percha and either a zinc oxide-eugenol-based sealer or AH26 to determine what effect these sealers, with widely differing properties, would have on apical microleakage after either immediate or delayed dowel space preparation. The teeth were randomly assigned to 1 of 4 groups, and the post space was made either immediately after filling or after being stored in 100% humidity for 1 wk. They were then immersed in a 2% methylene blue solution under vacuum, washed, and split longitudinally. The extent of dye penetration was read by five independent observers and the results analyzed. The only significant difference was in the delayed preparation group with zinc oxide-eugenol sealer, which showed greater leakage than the other groups.

Repair of furcal perforations with mineral trioxide aggregate: Two case reports

Furcal perforation is an unfortunate incident that can occur during root canal therapy or post preparation of multirooted teeth. Studies have shown that the materials currently used to repair these iatrogenic accidents are inadequate. The poor prognosis of furcation perforations is probably due to bacterial leakage or lack of biocompatibility of repair materials. On the basis of the recent physical and biologic property studies of the newly introduced mineral trioxide aggregate, this material may be suitable for closing the communication between the pulp chamber and the underlying periodontal tissues. These case reports support this hypothesis.

Coronal Leakage: Bacterial Penetration through Obturated Canals Following Post Preparation

Coronal leakage of bacteria from saliva into root canal filling materials is a potential cause of failure. This problem may be more pronounced when only a small volume of obturating material remains in the canal, such as after post preparation. In this study, coronal leakage of bacteria through unsealed, apically obturated canals was investigated using a new in vitro model system.

Effect of dowel space preparation on the apical seal of root canal fillings

The effect of post preparation on the apical seal was studied using simulated root canals. The maximum level of removal was tested with residual fillings 1, 2, 3, and 4 mm long. All of the artificial canals were filled with gutta-percha, Grossman's sealer, and lateral condensation. The removal was immediate and carried out with warm pluggers and the dowel space was completed with a Peeso drill. Stainless steel posts were cemented with zinc phosphate cement in the free space of the root canals. The coronal access of the canals was sealed with temporary fillings. After setting the materials, the specimens were immersed in a 2% methylene blue dye solution for 72 h and were observed with a measuring microscope. The final length of the apical fillings was found to be different from the intended length in every group. As far as leakage was concerned, 1, 2, and 3 mm-long fillings did not show a significant difference when compared with the negative control group (that is to say, filled canals where no dowel space was created). In the 4 mm fillings the leakage value was zero. These findings may be of clinical importance when restoring short roots.

Apical sealing ability of thermafil following immediate and delayed post space preparations

Sixty extracted human teeth were prepared in a step-back flare method. Forty-five were obturated with plastic Thermafil and 15 were filled with lateral condensation. The Thermafil filled teeth were divided into three groups consisting of no post preparation, immediate post preparation, or delayed post preparation. Post preparations were not performed (controls) on the laterally condensed specimens. After 10 days of exposure to methylene blue dye, the specimens were decalcified in nitric acid for 72 h. A volumetric analysis measuring absorbance was then performed on each specimen. Within the testing parameters, no significant differences in leakage were observed among the tested groups.