Post-mortem Inspection Research Articles

PEMERIKSAAN ANTE-MORTEM DAN POST-MORTEM HEWAN KURBAN DI MUSHOLLAH AL-FAIDAH RSS OESAPA KOTA KUPANG TAHUN 2022

To ensure the safety of food components derived from animals, it is necessary to conduct inspections of animals used as sacrifices. ante-mortem and post-mortem inspections are conducted to ensure the safety of meat that will be distributed to the public. The rising number of sacrificed animals at mosques and prayer rooms in Kupang City during Eid al-Adha necessitates the recruitment of additional veterinarians to ensure the safety of the slaughtered animals' meat. In response to this need, the Animal Husbandry Service of the Kupang City Government, in collaboration with the Faculty of Medicine and Veterinary Medicine, conducted inspections of sacrificial animals in mosques and prayer rooms by conducting ante-mortem and post-mortem examinations of animal health and sacrificial meat. The ante-mortem and post-mortem examinations conducted at the Al-Faidah mosque provided positive results, and the final recommendation was that the sacrificed animals were healthy and in accordance with Islamic Sharia. The inspection results indicate that the meat is also suitable for human consumption.

Can we use meat inspection data for animal health and welfare surveillance?

Ante- and post-mortem inspections at abattoir were originally introduced to provide assurance that animal carcasses were fit for human consumption. However, findings at meat inspection can also represent a valuable source of information for animal health and welfare surveillance. Yet, before making secondary use of meat inspection data, it is important to assess that the same post-mortem findings get registered in a consistent way among official meat inspectors across abattoirs, so that the results are as much independent as possible from the abattoir where the inspection is performed. The most frequent findings at official meat inspections of pigs and beef cattle in Sweden were evaluated by means of variance partitioning to quantify the amount of variation in the probabilities of these findings due to abattoir and farm levels. Seven years of data (2012-2018) from 19 abattoirs were included in the study. The results showed that there was a very low variation between abattoirs for presence of liver parasites and abscesses, moderately low variation for pneumonia and greatest variation for injuries and nonspecific findings (e.g., other lesions). This general pattern of variation was similar for both species and implies that some post-mortem findings are consistently detected and so are a valuable source of epidemiological information for surveillance purposes. However, for those findings associated with higher variation, calibration and training activities of meat inspection staff are necessary to enable correct conclusions about the occurrence of pathological findings and for producers to experience an equivalent likelihood of deduction in payment (independent of abattoir).

Prevalence of Fasciolosis and Cystic Echinococcosis in Slaughtered Cattle in Abattoir of Al-Nassiriyah City

Thepresent study was conducted from January to May 2018 to identify the infection rate of fasciolosis and cystic echinococcosis in cattleslaughtered in the slaughterhouse of Nassiriyah Municipality, Thi-Qar province. During this study, 524 (247 males and 277 females) cattle were submittedfor postmortem inspection. Chi-square is used to analyze the data. The prevalence of fasciolosis in the examined cattle was (7.25%) while the rate of infection with cystic Echinococcosis was (8.40%) with no significant difference.Infection with fasciolosis and cystic echinococcosis was higher infemales than males.The current study showed significant relevance between the sex of cattle andthe prevalence of both fasciolosis and cystic Echinococcosis (P?0.05). The prevalence of fasciolosis and cystic Echinococcosis were significantly affected among the age groups in cattle (P?0.05).

Prevalence of Hydatidosis in Cattle, Sheep and Goats slaughtered in Haramaya Municipal Abattoir Eastern Part of Ethiopia

A cross sectional study was carried out from November 2019 to March 2020 with the aims of determining the prevalence and associated risk factors for transmission of the cystic echinococcosis between ruminant animals, and organ distribution of cysts in cattle, sheep and goats at Haranya municipal abattoir, eastern Ethiopia. During this study, 298 cattle, 164 sheep and 88 goats’ carcass were examined thoroughly for the occurrence of the disease and Cattle, 47(15.8%), sheep 31(18.9%) and goats 3(3.4%) were found to be positive for hydatid cyst by postmortem inspection. Out of examined organs in all species (livers, lungs, kidney and hearts), the infection prevalence was higher in lung (60.1%) lung, than the rest of the organs whereas the liver is second affected organ in all the three species of animals with (p< 0.05) respectively. The prevalence and the number of cysts in ruminants were found different when the cattle, sheep and goats examined were selected randomly based on their origin, sex, age, and body condition. The Highest prevalence was observed (P<0.05) in all cattle, sheep, and goats’ of poor body condition (19.7%), followed by medium (17.7%) and good body condition (10.5) respectively. There was also a statistically significant difference (P<0.05) in the prevalence of ruminant hydrosis in different age groups considered. The highest prevalence was in adult (16.9%) animals and the lowest (10.5%) was found in young animals. The prevalence of hydatidosis recorded among animal origins with Haranya, Babile, Kersa and Kombolcha were (17.5%), (15.8%) (15.3%) (11.7) respectively and no statistical variation was observed (P> 0.05). However statistical significance variation (P = 0.001) was observed in the prevalence of hydatidosis among female and male. The result showed that out of 153 cysts assessed (56.8%), (31.4%) 18(12%) were fertile, sterile and calcified respectively. Among the 87 fertile cysts, 54(62.1%) and 33(37.9%) of them were found to be viable and non-viable respectively. The present study showed that hydatidurias is considerably prevalent disease of ruminants in the study area. Therefore, it needs to avoid backyard slaughter practice, unsafe offal feeding of dogs, and proper west disposal. Moreover, Public awareness on the use of abattoirs.

Evaluating remote post-mortem veterinary meat inspections on pig carcasses using pre-recorded video material

BackgroundOfficial meat inspections at small-scale slaughterhouses and game-handling establishments in geographically remote areas place a heavy burden on the meat-producing food business operators. By performing meat inspections remotely using live-streamed video, instead of on-site, the official control could meet the goals of sustainability, resilience and logistics. We investigated the agreement between the two approaches at pig slaughter. Two official veterinarians (OVs) inspected 400 pig carcasses at a Swedish slaughterhouse, with each pig being inspected on site by one OV and remotely by the other. After a period of 3 to 6 months, video recordings of the remote inspections were assessed again by the same OVs, thus enabling direct comparisons of previous on-site inspections and renewed video-based inspections within the same OV.ResultsAgreement across 22 finding codes was generally very high for both OVs. In all but one case (whether to fully condemn a carcass), for both OVs, Prevalence-Adjusted Bias-Adjusted kappa was well above 0.8, indicating ‘almost perfect agreement’.ConclusionsThis study supports earlier findings that reliable post-mortem inspections can be performed using video, and indicates higher agreement between remote and on-site inspections if the same OV performs both.

Prevalence of Fasciola species from Cattle Slaughtered at Birnin Kebbi Modern Abattoir

Diagnostic tools to detect and differentiate Fasciola species have improved, but the understanding of the distribution and population structure of this parasite is location dependent. This study was aimed to phenotypically identify Fasciola species from cattle brought for slaughter at Birnin Kebbi Modern Abattoir. Ante-mortem faecal samples were collected from the sampled cattle for worm egg detection and post-mortem inspection of liver samples were performed. Two methods of Parasitological (Flukefinder Kit and formalin-ether sedimentation) methods were performed on the faecal samples. Results have yielded a high prevalence (31.2%) of fluke eggs with the Flukefinder Kit than with formalin-ether sedimentation technique (8.5%). Prevalence was higher (34%) in the males than in female (26.2%) cattle. Younger cattle (34.3%) were also more infected than their older (30.7%) counterpart. Sokoto Gudali had the highest prevalence of 44.0% followed by Red Bororo with 34.0%, and the least prevalence was observed in Bunaji breed with 18.0%. Statistical analyses results had revealed no significant relation between prevalence of fasciolosis and the co-variables (sex, age) as p > 0.05 but there was a significant association between prevalence and breed of cattle (p = 0.018). There was no significant agreement between the methods of faecal analyses used. Prevalence of 15.8% was observed from the post-mortem examination. This have increased the knowledge about the existence of the parasite in the study area. It is recommended that the Flukefinder® Kit should be used in diagnosis of Fasciola species due to its high sensitivity compared to the conventional sedimentation method.

Assessment of bovine fasciolosis and the analytical test evaluation at Yirgalem municipal abattoir, Ethiopia

A cross-sectional study was conducted from November 2021 to April 2022 to estimate the prevalence of bovine fasciolosis, assess the risk factors, and estimate the direct financial loss due to liver condemnation in cattle slaughtered at Yirgalem municipal abattoir. Furthermore, the comparison of the diagnostic efficiency of fecal and postmortem examination was evaluated. The study was based on both postmortem inspection of the liver for liver fluke infection and coprological examination using the sedimentation technique. Thus, a total of 400 cattle were sampled using systemic random sampling techniques and the prevalence was 27.0% (108/400) and 20.8% (83/400), by postmortem and coprological examination, respectively. The prevalence of F. hepatica, F. gigantica, mixed infections, and unidentified species were 56.5%, 19.4%, 16.7%, and 7.4%, respectively. The difference between age groups was statically significant (p<0.05). Coprological examination revealed the highest prevalence was recorded in the age group of > 7 years old (34%, adjusted odds ratio [AOR]=4.2) followed by 4-6 years old adults (26.8%, AOR=4.2) and in young cattle of <3 years old (6.7%). Prevalence by postmortem examination was 43.0% (AOR=4 0), 27.8% (AOR= 2.4), and 8.3%, for age groups > 7 years old, 4-6 years old adults and in young cattle of <3 years old, respectively. The body condition score result revealed a significant difference (p<0.05) as a greater magnitude of infections were detected in poor body condition animals (AOR=7.7) than in medium and good body condition (p<0.05). Considering the liver examination as a gold standard for diagnosis of fasciolosis, the sensitivity of the sedimentation technique was 76.8%, and the specificity of 100% with a very good agreement (kappa = 0.94) between the two methods. The total financial loss per annum due to the condemnation of infected liver was 945, 999 ETB (18,192.288 USD). Therefore, strategic control and prevention of the snail intermediate host should be implemented. Furthermore, upon diagnosis of fasciolosis, epidemiological information about the disease with suggestive clinical examination should be considered even in the absence of Fasciola eggs during coprological examinations and also other parasitological techniques such as the FLOTAC and FLUKE FINDER methods with high sensitivity and specificity should be employed.

Financial Losses Arising from Cattle Organ and Carcass Condemnation at Lokoloko Abattoir in Wau, South Sudan.

Slaughterhouses in South Sudan mirror the economic losses resulting from cattle organs and carcass condemnation due to zoonotic and epizootic diseases of livestock, such as tuberculosis, cysticercosis, and hydatidosis in cattle. However, due to the war, slaughterhouse record keeping has been inconsistent in South Sudan, and thus the estimation of diseases in cattle and their impact may be underestimated. Therefore, this study was conducted to estimate the major causes of carcasses and organ condemnation of cattle slaughtered at Lokoloko abattoir and the resulting financial losses. A cross-sectional active abattoir survey involving antemortem and postmortem examinations was conducted on 310 cattle between January 2021 and March 2021. Furthermore, five-year (September 2015-September 2020) retrospective data on meat inspection records were also collected and analyzed. During the antemortem inspection of the active abattoir survey, 103 (33.2%) cattle had signs of disease. These signs included herniam 17 (5.5%), local swelling 16 (5.2%), lameness 15 (4.8%), emaciation 13 (4.2%), blindness 12 (3.9%), depression 11 (3.5%), pale mucus membrane 7 (2.3%), nasal discharge 5 (1.6%), lacrimation 4 (1.3%), and salivation 03 (0.97%). Postmortem inspection revealed gross pathological findings on 180 (58.6%) carcasses, out of which 47 (26.1%) livers and 31 (17.2%) hearts were condemned due to various causes. The active abattoir survey and the retrospective data revealed that tuberculosis, fascioliasis, hydatidosis, and heart cysticercosis were the leading causes of condemnation of carcasses and organs. In the active abattoir survey, a total of 19,592,508 South Sudanese Pounds, equivalent to US$29,686 was lost from organ condemnation, while in the retrospective data; the overall direct financial loss during the five years was estimated to be 299,225,807 South Sudanese Pounds equivalent to US$453,372. This study revealed that bacterial and parasitic diseases were the common causes of carcass and organ condemnations and caused significant financial losses at Lokoloko abattoir in Wau, South Sudan. Therefore, there is a need for training farmers on cattle disease management, heightened meat inspections, and proper disposal of condemned meat.

Ante-mortem and Post-mortem Inspection and Relationship between Findings in a North Albanian Pig Slaughterhouse.

In June 2014, Albania was granted EU candidate status, thus starting a process of compliance with the membership criteria. In this context, a modern meat inspection approach in line with the European legislation was applied to a pig slaughterhouse in northern Albania in order to investigate the ante-mortem (AM) and post-mortem (PM) conditions and the relationship between these findings. For this purpose, 3930 pigs divided into 35 batches were evaluated over a 3-month period. The most frequent AM conditions recorded were tail lesions and dyspnea (9.1%), followed by skin (8.9%) and ear lesions (8.5%), while in the PM inspections, pleuritis was the most frequently observed condition (10.2%), followed by pneumonia (8.5%), liver alterations (5.7%), milk spot liver (3.8%), and pericarditis (3.3%). With the exception of liver alterations, the other PM lesions mentioned were positively associated with lesions on the ears (OR = 1.036; p < 0.001) and skin (OR = 1.026; p = 0.011) and dyspnea (OR = 1.021; p = 0.005), confirming the link between these variables and the health and welfare conditions of pigs on farms. Overall, the evidence that emerged from this Albanian slaughterhouse can be considered in line with other European contexts, especially in light of the considerable variability in the data present in the literature.

Pre-slaughter, slaughter and post-slaughter practices of slaughterhouse workers in Southeast, Nigeria: Animal welfare, meat quality, food safety and public health implications.

Pre-slaughter stress or the welfare condition of food-producing animals (FPAs) and the slaughter practices of slaughterhouse workers (SHWs) are critically important for the safety and quality of meats processed in slaughterhouses (SHs). Consequently, this study determined the pre-slaughter, slaughter, and post-slaughter (PSP) practices of SHWsin four SHs in Southeast, Nigeria; and discussed the impacts on meat quality and safety. The PSP practices were determined by observation method. Additionally, a structured and validated closed-ended questionnaire was used to determine the knowledge of the SHWs on: the effects of poor welfare (preslaughter stress) on the quality and safety of meats produced, carcass/meat processing practices and modes of transmission of meat-borne zoonotic pathogens during carcass/meat processing. Finally, a systematic post-mortem inspection (PMI) was conducted on cattle, pigs and goats slaughtered, and economic losses accruable from condemned carcasses/meats were estimated. Food-producing animals were transported to the SHs or held in the lairage under inhumane conditions. A pig being conveyed to one of the SHs was seen gasping for air, as it was firmly tied on motorbike at the thoracic and abdominal regions. Fatigued cattle were forcefully dragged on the ground from the lairage to the killing floor. Cattle for slaughter were restrained, held in lateral recumbency and left groaning, due to extreme discomfort, for about one hour before slaughter. Stunning was not performed. Singed pig carcasses were dragged on the ground to the washing point. Although more than 50% of the respondents knew the modes of transmission of meat-borne zoonotic pathogens during meat processing, 71.3% of the SHWs processed carcasses on bare floor, 52.2% used same bowl of water to wash multiple carcasses while 72% did not wear personal protective equipment during meat/carcass processing. Processed meats were transported to meat shops in an unsanitary conditions, using open vans and tricycles. During the PMI, diseased carcasses/meats/organs were detected in 5.7% (83/1452), 2.1% (21/1006) and 0.8% (7/924) of the cattle, pig and goat carcasses inspected, respectively. Gross lesions pathognomonic of bovine tuberculosis, contagious bovine pleuro-pneumonia, fascioliasis and porcine cysticercosis were detected. Consequently, 391,089.2 kg of diseased meat/organs valued at 978 million Naira (235, 030 USD) were condemned. There were significant associations (p < 0.05) between educational level and the use of personal protective equipment (PPE) during slaughterhouse operations and knowledge that FPAs can harbour zoonotic pathogens (p = < 0.001) transmissible during carcass processing. Similarly, significant association was observed between working experience and use of PPE; and between geographical location of the respondents and knowledge that zoonotic pathogens in animals are transmissible during carcass processing or via the food chain. The findings show that slaughter practices of SHWs have detrimental impacts on the quality and safety of meats processed for human consumption in Southeast, Nigeria. These findings underscore the need to: improve the welfare condition of slaughter-animals, mechanise abattoir operations, train and retrain the SHWs on hygienic carcass/meat processing practices. There is a need to adopt strict enforcement of food safety laws to promote meat quality, food safety and consequently promote the health of the public.

Occupational Health Hazards and Safety Practises on Abattoir Workers in Owerri Metropolis, Imo State

The purpose of this study was to determine the impact of occupational health hazards and safety practises on abattoir workers in Owerri Metropolis, Imo State, Nigeria. For the study, three objectives and three questions were developed. This study used a descriptive survey design. The population of the study consisted of 232 abattoir workers recruited from the three major abattoirs in Owerri Metropolis, Imo State. The study's sample included all two hundred and thirty-two (232) abattoir workers from the three major abattoirs in Owerri Metropolis. At the 0.05 level of significance, inferential statistics of Chi square (x2) were used to analyse the data collected for hypotheses, while the objectives were presented in frequency and percentages. According to the findings, the major occupational health hazards include knife cuts, blood/waste splashes, noise, bone piercing, and chemical spill, burns, and irritation. Adopted safety practises include the use of an apron, gloves, boots, and pre- and post-mortem inspection. Based on the findings, it was recommended that the government, through the ministry of health and labour, develop or create survey instruments and conduct surveys on a regular basis to monitor the prevalence of occupational health diseases and the upkeep of safety practises in abattoirs.

CARCASSES AND OFFAL CONDEMNATION AT KOM-ELNOUR ABATTOIR IN DAKAHLIA PROVINCE, EGYPT: MAJOR CAUSES AND ECONOMIC LOSS

An abattoir survey was conducted from October 2020 to September 2021 to identify the primary reasons for offal and carcass condemnation of slaughtered animals in Dakahlia province, Egypt, as well as to estimate their economic loss. During the survey, 643 animals (441 cattle, 178 buffalos, 19 camels, 4 sheep, and one goat) were submitted for antemortem and postmortem inspection. The results of both inspections were collected, analyzed, and the total economic loss was estimated by adding the condemnation of offal and carcasses over a twelve-month period. Retail prices for offal (liver, lung, heart, and tongue) and carcass prices per kg were obtained from local markets. Out of 643 animals slaughtered, 2 (0.31%) were totally condemned. In addition, postmortem inspection revealed that 217 (33.74%) livers, 158 (24.57%) lungs, 5 (0.78%) hearts, 14 (2.18%) heads, and 11(1.71%) tongues were condemned. Offal were condemned mainly due to pneumonia, fascioliasis, telangiectiasis, necrosis, and hydatidosis, whereas carcasses were rejected due to generalized tuberculosis. According to this study, the financial loss at the abattoir owing to carcass and organ condemnations was 244066 Egyptian Pounds over a twelve-month period (15746 USD). The incidence of meat rejection and subsequent financial loss was high compared to the amount of local revenue. The current study concluded that bacterial and parasitic diseases are still prevalent and cause significant economic damage in Dakahlia province, Egypt. This abattoir survey offered regional information on the principal reasons for carcass and organ condemnation in slaughtered animals, as well as an estimate of the direct economic consequences. Furthermore, the findings of the current work underlined the importance of developing an efficient monitoring system for meat condemnation and enforcing animal health strategies in Egypt.

The Importance of the Slaughterhouse in Surveilling Animal and Public Health: A Systematic Review.

From the point of public health, the objective of the slaughterhouse is to guarantee the safety of meat in which meat inspection represent an essential tool to control animal diseases and guarantee the public health. The slaughterhouse can be used as surveillance center for livestock diseases. However, other aspects related with animal and human health, such as epidemiology and disease control in primary production, control of animal welfare on the farm, surveillance of zoonotic agents responsible for food poisoning, as well as surveillance and control of antimicrobial resistance, can be monitored. These controls should not be seen as a last defensive barrier but rather as a complement to the controls carried out on the farm. Regarding the control of diseases in livestock, scientific research is scarce and outdated, not taking advantage of the potential for disease control. Animal welfare in primary production and during transport can be monitored throughout ante-mortem and post-mortem inspection at the slaughterhouse, providing valuable individual data on animal welfare. Surveillance and research regarding antimicrobial resistance (AMR) at slaughterhouses is scarce, mainly in cattle, sheep, and goats. However, most of the zoonotic pathogens are sensitive to the antibiotics studied. Moreover, the prevalence at the slaughterhouse of zoonotic and foodborne agents seems to be low, but a lack of harmonization in terms of control and communication may lead to underestimate its real prevalence.

In situ melting phenomena on W plasma-facing components for lower divertor during long-pulse plasma operations in EAST

Tungsten (W) is one of the most promising plasma-facing materials for future fusion devices. Although its melting point is the highest among all metals, it still has great risk of melting under extremely high plasma heat fluxes, which is a big concern for ITER and future reactors. Actively cooled W plasma-facing components (PFCs) with both monoblocks and flat-type structure have been successfully installed in the lower divertor of the EAST tokamak since 2021, and provide a good opportunity for direct comparison of the damage mechanism for the two types of PFCs. Various in situ melting phenomena on the lower divertor have been observed by CCD cameras, which have been further verified by post-mortem inspections. Severe melting and even exfoliation of the edge-beveled W plates were observed on some W/Cu flat-type components at horizontal outer targets. Many droplets were ejected during long-pulse operations, which induced a significant increase of W impurities and total irradiation in the core plasma, and thus greatly deteriorated the plasma performance and even caused disruptions. Two different shaping structures of flat-type PFCs show different positions of melting and the corresponding mechanisms. Slight melting was found on the sharp leading edges of W/Cu monoblocks between cassette modules (inter-CM) for horizontal targets with small droplet ejection, which was much improved compared to that observed on the upper W divertor, illustrating that the application of a large-sized bevel chamfer inter-CM was generally effective. In addition, an unexpected melting phenomenon on the dome plate was attributed to the extreme transient heat flux during disruption with runaway electrons. The application of both types of W/Cu PFCs for the divertor provides important experiences and lessons for the engineering design and optimization of divertor PFCs in future fusion devices.

The Value of Current Ante Mortem Meat Inspection and Food Chain Information of Dairy Cows in Relation to Post Mortem Findings and the Protection of Public Health: A Case for a More Risk-Based Meat Inspection.

In this study, the contribution of the ante mortem (AM) inspection and the food chain information (FCI) to ensuring meat safety and public health was investigated, by evaluating the slaughterhouse findings of 223,600 slaughtered dairy cows in the Netherlands. The outcome of this study was that the ante mortem (AM) and post mortem (PM) inspections have a substantial overlap, and that with regard to food safety and public health in over 99% of cases the PM could even be omitted on the basis of the AM. In this study, the data provided by the dairy farmers on the current FCI forms contributed little to nothing with regard to the outcomes of AM and PM inspection. It is concluded that current meat inspection procedures need an update and a more risk-based approach needs to be adopted. Regarding this, the AM inspection of dairy cattle should remain, because it plays an important role in ensuring food safety (e.g., by preventing contamination of the slaughter line by excessively dirty animals, or animals with abscesses), monitoring animal welfare and in detecting some important notifiable diseases. The PM inspection, however, could in many cases be omitted, provided there is a strict AM inspection complemented by a vastly improved (automated) way of obtaining reliable FCI.

Effects of post-mortem inspection techniques change on the detection capability of low public health impact diseases of slaughtered pigs: A quasi-experimental study.

Slaughtered animals are regularly submitted to post-mortem inspection to ensure that all the edible parts are fit for human consumption. According to Regulation (EU) No 219/2014, pig carcasses inspection is exclusively visual as palpation and incision could lead to cross-contamination and spread of relevant zoonotic agents. However, when compared to incision and palpation, the visual method is characterized by low sensitivity; thus, the omission of incision and palpation could lead to a reduced detection capability of organic lesions. This study aims to assess the effectiveness of exclusively visual inspection to mark pulmonary and hepatic lesions associated with low public health impact diseases in pork carcasses. A quasiexperimental- before/after research protocol has been used. All the post-mortem inspections have been carried out in a slaughterhouse located in the province of Teramo (IT), on 7,764 swine from 2011 to 2017. Carcasses undergone the only visual inspection have shown a statistically significant reduction (pvalue <0.0001) in the diagnosis of hepatic (decrease of 59%) and pulmonary diseases (decrease of 38. 5%). To overcome the limits of the low sensitivity of the visual inspection, as well as the inter-operator diagnostic variability, the high number of carcasses examined is proposed as a factor conferring external validity to the study, which provides quantitative evidence in support of the causal association between the modified inspection technique and the reduced diagnostic capacity. A further support derives from the assessment of the prevalence of hepatic and pulmonary diseases in species for whom the inspection technique is not changed.

Sudden Unexpected Death Due to Myocarditis Caused by Coronavirus Disease 2019: Postmortem Histopathologic Evaluation

Coronavirus disease 2019 (COVID-19) is a manifestation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and its major symptoms include pulmonary complications, such as pneumonia. However, it also involves the cardiovascular system and the developed myocarditis can lead to sudden unexpected death. Herein, we present a case in which a patient died four days after release from isolation due to SARS-CoV-2 infection. SARS-CoV-2 was confirmed again during postmortem (PM) inspection at the scene of death. Autopsy revealed myocarditis and evidence of pulmonary involvement with SARS-CoV-2. Pathological examination revealed myocardial perivascular infiltration of lymphocytes and macrophages with multifocally injured cardiomyocytes. The pathological findings of COVID-19–induced myocarditis differ from those of other viral myocarditis, and we assume that different pathophysiological mechanisms could have been responsible for this manifestation. After a comprehensive PM examination, including gross dissection, microscopic examination, PM computed tomography, and PM laboratory tests, the cause and manner of death were determined to be myocarditis caused by COVID-19 and naturally, respectively. This case highlights the significance of autopsy and comprehensive PM examinations in both forensic and public healthcare systems.

Bovine Tuberculosis - Analysis of 10-year cases and impact of visual inspection in the surveillance at the slaughterhouse in Portugal

Bovine Tuberculosis (bTB) is a One Health issue that interconnects human health with animal and environmental health. Due to its importance, cattle (the main host) it is under a national eradication programme in Portugal. Within it, meat inspection is considered a cost-effective procedure in bTB surveillance. The Commission's Implementing Regulation (EU) 2019/627 changed the post-mortem meat inspection requirements applied to cattle, essentially reducing handling and incisions in young cattle (<8 months). To study the impact of these changes on the surveillance of bovine Tuberculosis, data (age, sex, fitness, breed, lesions, laboratory results and post-mortem decisions) on suspected and confirmed cases of bTB detected at routine slaughter of the period from October 2010 to January 2020 were analysed.From a total of 3,279,548 slaughtered animals, meat inspection detected 808 suspected cases. Of these, 381 cases were laboratory confirmed. The estimated global prevalence was 11.6 cases/100,000, increasing with age: 3.5, 5.9 and 27.3 cases/100,000 in, respectively, the groups <8 months, 8 to 19 months, and ≥ 20 months. The prevalence was higher in females (16.3 vs 7.3 cases/100,000 in males) and in beef cattle (16.3 vs 1.4 cases/100,000 in dairy cattle). Among the confirmed cases, mediastinal and bronchial lymph nodes were the most reported locations. Based on the results, there is no evidence of a negative impact on the application of Regulation (EU) 2019/627 on bTB surveillance in animals ≥8 months. For the <8 months group the change to a visual inspection protocol could lead to a failure in the detection of bTB cases. Considering the epidemiological situation, the incision of the mediastinal, bronchial and retropharyngeal lymph nodes in cattle <8 months should be maintained.

Stochastic partial budget analysis of strategies to reduce the prevalence of lung lesions in finishing pigs at slaughter.

Ante- and post-mortem inspections of food-producing animals at slaughter are mandatory activities carried out in many countries to ensure public health, animal health, and meat quality. In finishing pigs, lung lesions are the most frequent defects found in meat inspections. It is possible to implement managerial strategies on-farm to reduce the occurrence and spread of respiratory diseases, but such strategies come with additional costs that could impede implementation. This study assessed the economic impact of two strategies aimed at reducing the prevalence of lung lesions in finishing pigs at slaughter by improving the health conditions of the animals during the production cycle. First, a farrow-to-finish pig farm with 355 sows was modeled based on the current standard practice for finishing pig production in Sweden, using economic data, meat inspection data and biological variables from the literature and expert opinions. A partial budget analysis was then performed in which the baseline farm was compared with two hypothetical strategies aimed at reducing the occurrence and spread of respiratory diseases during pig production: (S1) avoiding mixing of litters after weaning and (S2) keeping purchased pregnant gilts separated from sows during gestation, farrowing and lactation. Both these strategies intended to reduce the occurrence of respiratory disease in finishing pigs at slaughter gave an average gain in annual net income (33,805 SEK in S1 and 173,160 SEK in S2, equal to 3,146€ and 16,113€, respectively, at the time of analysis), indicating that both were economically sustainable under the assumed conditions. The impact analysis of the two strategies revealed that the reduced prevalence of lung lesions when adopting one of the strategies was the most influential factor in net benefit change on the farm. Overall, the results suggest that with the increasing prevalence of lung lesions in Swedish pig production (as also observed worldwide in recent years), adopting an effective strategy to decrease respiratory infections will become more relevant and economically beneficial.

A preliminary investigation into the automatic detection of diseased sheep organs using hyperspectral imaging sensors

The post-mortem inspection process of livestock viscera at abattoirs is expensive and laborious, but it is essential for the detection and condemnation of defective edible organs and carcases due to food safety issues. Lesions in hearts, kidneys, livers, lungs, and their associated lymph nodes are amongst the most common offal defects found in abattoirs. Visible (VIS) and short-wave infrared (SWIR) hyperspectral imaging implemented in a multisensory platform were used to differentiate between sheep parenchymatous organs passed as fit (Healthy, n = 42) or not fit (Diseased, n = 47) for human consumption. Partial least squares discriminant analysis (PLS-DA) and random forest (RF) were used to classify organs as healthy or diseased in heart (n = 28), kidney (n = 15), liver (n = 24), and lung (n = 22). PLS-DA produced equal or greater classification accuracy and sensitivity than RF for all organs except for lung when the VIS sensor was used (means 84.4% and 78.3%, respectively). Livers and hearts (86.9%) showed higher accuracy than lungs and kidneys (75.9%). Limited differences occurred between VIS and SWIR sensors, although a single sensor tended to be more accurate than a combination of both. SWIR outperformed VIS in accuracy across all organs (84.8% vs. 76.3%), and the combination of VIS and SWIR was also accurate (83.0%). The use of hyperspectral imaging is an attractive proposition for the meat processing industry as a non-invasive imaging technology to detect defects in offal, and it can also provide automatic detection, saving time and labour costs.