The current study was to estimation the post-harvest loss of avocado at the producer level using cross-sectional data obtained from 385 proportionately sampled respondents from Wolaita and Kembata Tembaro Zones, the SNNPR of Ethiopia. Descriptive and multiple regression analysis were used to estimate the amount and determinant of post harvest loss. The average post-harvest loss of avocado at the producer level was estimated at 24.1 per cent, of which 5.7 per cent was due to poor harvesting practices and 4.9 per cent to poor storage practices. Sex, education, income, training, contact with extension agents, distance from the nearest road, and off-farm income were found to be negative determinants, whereas storage accessibility and avocado harvest techniques were found to be positive determinants of post-harvest loss of avocado fruits. Post-harvest losses contribute significantly to food insecurity and create a problem for the income source of individuals and the economy of the country.