Abstract

Aflatoxins are the major and global concern in food and feed matrices. These fungi affect cereals, nuts and spices commodities during the pre and post harvesting treatments, handling and storage. The fungal isolates harbour within the crop or food material and lead to the production of toxin in these materials. Soil borne pathogenic fungi are responsible for significantly decreasing yield by affecting the crop. Aspergillus spp. is the prominent pathogen responsible for Aflatoxin contamination. Chemical control of this fungal pathogen may be harmful or can cause dangerous effects on consumers as well as on soil quality. However, the use of biological methods in controlling this plant pathogen is a beneficial technique for ecology and environment. So, these biocontrol agents are recommended as these are eco-friendly and reduce the growth of fungal pathogens and contaminants. Considering this in our current investigation, we have studied in vitro antagonistic activity of biocontrol agent- Trichoderma asperelloides against the aflatoxin producing fungi isolated from peanut. The study was carried out by performing the dual culture technique. Further the medium was also analysed for Aflatoxin content by using Ultra High-Performance Liquid Chromatography with Fluorescence Detection (UHPLC-FLD) technique.

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