College students experience increased stress levels that could predispose them to develop mental and physical health conditions throughout adulthood. Yoga is an ancient mind-body practice including breath techniques, body exercises, and meditation that may be a useful strategy for enhancing college student health. Likewise, exposure to nature has been shown to have beneficial impacts on human health. This study investigates physiological and psychological responses of college students to yoga practiced in outdoor versus indoor environments. Fifty-eight college females between the ages of 18-28 were randomly assigned to complete a 40-minute Hatha yoga session either indoors (empty classroom) or outdoors (green park) at a mid-sized public university. Heart rate, respiration rate, perfusion index, and positive and negative affect states were recorded pre/post yoga. Heart rate, respiration rate, and negative affect significantly improved after the yoga sessions in both indoor and outdoor environments. Baseline self-reported negative affect was significantly lower in the outdoor group compared to the indoor group. There were no significant interaction effects of yoga and environment. This study suggests that one 40-minute-session of Hatha yoga may be a useful method to alleviate acute signs of physiological stress and decrease negative affect for college females in both indoor and outdoor environments. Further research about potential synergistic effects of yoga and exposure to nature is warranted to better understand whether the environment in which yoga is practiced could enhance the benefits of this mind-body modality on health.