The acute hepatic phase response is defined as a reaction that includes hepatic synthesis of proteins, consisting in the increase of some proteins called positive acute phase proteins and the simultaneous decrease of others called negative acute phase proteins. This study describes this hepatic reaction, based on a series of consecutive determinations, at three different time intervals (right before a major surgery event, 24 and 48 hours after the intervention), of the plasmatic levels of transferrin, albumin, fibrinogen and C reactive protein (CRP). Subsequently, the data was analyzed using Jamovi 2019, version 0.9. The inferential statistics consisted in calculating an ANOVA test that compared the values at 24 hours and 48 hours versus the values right before the major surgery. The results were validated by calculating the p value (p[0.05) as well as conducting correlation tests by determining the Pearson coefficient which shows the values of CRP, fibrinogen, transferrin and albumin are independent of each other and do not interact. The multiple ANOVA comparative test reveals the lack of interaction between the values of determined proteins, regardless of the moment of determination.