Portable Handheld Research Articles

Mobile guardians: Detection of food fraud with portable spectroscopy methods for enhanced food authenticity assurance

It is often said that “you are what you eat”, and whether this is said in this decade or in 2050 the choices we make about the food we consume can alter our biology. Therefore, knowing exactly what you eat is important for one to maintain a healthy balanced diet. However, as the food industry grows in complexity and struggles to meet the demand of a rapidly increasing population, it is likely that food fraud will pose a much larger threat to the future safety of food industries and consumers. Thus, it is necessary to employ and develop analytical techniques such as infrared and Raman spectroscopy as mobile ‘Capable Guardians’ to reduce this potential risk. Recent advancements in portable spectroscopic instrumentation which can provide rapid on-site measurements show promise, and may have a pivotal role to play in the ongoing saga of food fraud and contamination. Therefore, the objective of this review is to present a comprehensive overview of food fraud and contamination, highlighting the common analytical methods employed for their assessment, with a specific emphasis on the utility of portable handheld spectroscopic instrumentation which in the future can offer guardianship and thus ensure personalised food security.

The Threat of Saltwater Intrusion on Rice Farming in Katingan I Lowland Irrigation Area, Central Kalimantan

The Katingan I Lowlands Irrigation Area (LIA) is one of tidal lowlands irrigation area in Central Kalimantan, Indonesia. Because the Katingan I LIA utilizes water from the estuary, the occurence of saltwater intrusion is likely to occur. Assessing water salinity due to saltwater intrusion provides essential information for farmers to manage water appropriately. In addition, this information can also be used by the government to develop policies and management strategies for Katingan I LIA. Saltwater intrusion that occurs when sea water flows into irrigation canals and agricultural land can lead to increased salinity. High salinity levels can affect agricultural productivity, especially in rice crops. Therefore, a thorough salinity study is needed to support the development of rice farming. This study aims to evaluate salinity levels due to saltwater intrusion and understand the factors affecting them. The study measured tidal (water level elevation), electrical conductivity (EC), and salinity data of the Katingan River and several locations in the primary and secondary channels. Data collection methods included continuous and instantaneous measurements using portable data logger installations and handheld devices. The existing water salinity conditions at Katingan I LIA obtained the maximum water salinity value from the three locations of 14,536 ppm and the lowest water salinity value of 3.02 ppm. The maximum water salinity value that enters the primary canal is 5,547 ppm, higher than the thershold salinity value for rice farming (5,120 ppm). Therefore, anticipatory measuers must be conducted to reduce salinity intrusion, such as optitmizing water channels, making water gates, and managing the water use efficiently. It is also recommended that measurements be carried out during the dry season to see the comparison of results and the magnitude of the maximum water salinity value that occurs because salinity problems occur more during the dry season

Point-of-care device for the noninvasive assessment of hepatic macrosteatosis in liver donors

BackgroundQuantification of macrosteatosis (MS) in the liver is important given that it has shown to directly correlate with adverse post-liver transplant (LT) outcomes. With advances in medical technology and an implicit understanding of pathology, noninvasive methods of quantitatively assessing MS are in various stages of development. Each of these methods is based on the physical principles of differences between a fat-laden hepatocyte and a normal one. MethodsIn this regard, after a proof-of-concept study on a prototype for a simple, real-time, handheld device using the principle of diffuse reflectance spectroscopy, this study presents an upgraded point-of-care (POC) device for the noninvasive assessment of hepatic MS in liver donors. ResultsThe device was validated on cohort of donor livers and showed a sensitivity (0.0021 V/% fat) and highly correlated (r = 0.9868, P < .0001) with gold-standard liver biopsy. Results showed that this upgraded POC device provides a reliable method for the noninvasive assessment of hepatic MS, which is crucial for selecting suitable donor livers for LT. ConclusionThe device has the potential to be an invaluable apparatus at the hands of the organ-retrieving surgeon. It is noninvasive, portable (handheld), and economic; provides real-time readings of the percentage of MS; and can be efficaciously handled by any member of the organ-retrieving team.

Repeatability and reproducibility of quantitative OCT angiography measurements from table-top and portable Flex Spectralis devices.

The recent development of a portable investigational handheld OCT-angiography (OCTA) device has allowed for expansion of imaging into the operating room (OR) in addition to standard in-clinic imaging. The aim of this study was to assess intravisit repeatability and intervisit reproducibility of retinal microvasculature measures and central retinal thickness for in-clinic table-top and portable OR compatible OCTA devices. Repeated 10 × 10 OCTA images were acquired in 20 healthy adult participants on two separate visit days using Spectralis spectral-domain OCTA table-top and investigational armature suspended Flex systems. Intravisit and intervisit intraclass correlation coefficients and average absolute percent difference were calculated for quantitative microvasculature measures and CRT. 120 OCTA images were acquired from 20 subjects (n = 20, mean age 26.7 ± 1.61years, range 24-30years) with both devices across two separate imaging days. FAZ and CRT measurements had near complete intravisit and intervisit agreement with ICCs between .97 and 1 for both table-top (FAZ ICC .97, .97; CRT ICC .98-1, .98-.99) and Flex (FAZ ICC .97, .95; CRT ICC .99-1, .98-.99) devices. Vessel density measures demonstrated greater variance with only fair to strong agreement (ICC .32-.75) and average absolute percent differences ranging from 2.96 to 6.63%. FAZ and CRT measures for both devices demonstrated high repeatability and reproducibility; retinal vessel density measures demonstrated less. Differences of less than 7% for retinal microvasculature measurements across time and devices are most likely attributable to expectable variance between repeat scans.

Ionic Polyimine-Based Composite Membrane with Inductive and Complexation Synergistic Effects for Sensitive and On-Site Fluorescent Detection of Volatile Iodine.

Along with the development of nuclear power, concerns about radioactive emissions and the potential for nuclear leakage have been widely raised, particularly of harmful iodine isotopes. However, as a significant component of nuclear air waste, the enrichment and detection of air-dispersed gaseous iodine remain a challenge. In this work, it is focused on developing an attraction-immobilization-detection strategy-based fluorescence method for the on-site detection of volatile iodine, by employing a photoluminescent ionic polyimine network-polyvinylpyrrolidone (IPIN-PVP) composite membrane. This strategy synergizes ion-induced dipole interactions from IPIN and complexation effects from PVP, allowing effective iodine enrichment and immobilization. As a result, the optimized IPIN-PVP membrane exhibits rapid response times of 5 s and a low detection limit of 4.087 × 10-8m for gaseous iodine. It also introduces a portable handheld detection device that utilizes the composite membrane, offering a practical solution for real-time on-site detection of volatile iodine. This innovation enhances nuclear safety measures and disaster management by providing rapid and reliable iodine detection capabilities.

Portable and Cost-effective Handheld Ultrasound System Utilizing FPGA-based Synthetic Aperture Imaging

Portable and Cost-effective Handheld Ultrasound System Utilizing FPGA-based Synthetic Aperture Imaging

Unveiling Syndesmotic Malreduction: A Proof-of-Concept towards Portable Ultrasound Detection.

To evaluate the utility and diagnostic performance of portable handheld ultrasound for evaluating fibular rotation at the distal tibiofibular articulation after syndesmotic disruption. Four above-the-knee cadaveric specimens were included. Syndesmotic disruption was precipitated by transecting the Anterior Inferior Tibiofibular Ligament, Interosseous Ligament, and Posterior Inferior Tibiofibular Ligament. Thereafter, a proximal fibular osteotomy was performed, and three conditions were modeled at the distal syndesmosis: 1) reduced, 2) 5 degree internal rotation malreduction, and 3) 5 degree external rotation malreduction. Two blinded observers performed separate ultrasonographic examinations for each condition at the level of both the anterior and posterior distal tibiofibular articular surfaces. Syndesmotic gap penetrance, defined as the ability of the P-US to generate signal between the distal fibula and tibia at the level of the incisura, was graded positive if the sonographic waves penetrated between the distal tibiofibular joint and negative if no penetrating waves were detected. The accuracy measures of the anterior and posterior gap penetrance were evaluated individually. Our preliminary results showed that posterior gap penetrance showed good performance when detecting either internal or external rotational malreduction of the fibula with very good specificity (87.5%) and PPV (90.0%). On the other hand, the anterior gap penetrance showed limited performance when detecting either form of rotational malreduction. We introduced a novel sign, the "gap penetrance sign", best measured from the posterior ankle, which can accurately detect syndesmotic malreduction using P-US in a manner that does not require specific quantitative measurements and is readily accessible to early P-US users.

Assessment of Environmental Background Gamma Radiation Variation in Minna Area of Nigeria

Radiation has been acknowledged to be responsible for deleterious conditions in living tissues. Hence, environmental background gamma radiation (BGR) measurement is crucial from an environmental and health perspective. In this study, due to the absence of comprehensive background gamma radiation data and the increasing numbers of anthropogenic activities that could increase the BGR level, such as where mining activities are active. The BGR in the Minna area of Niger State, Nigeria, was measured and analyzed in this study. In-situ measurement of the background gamma radiation level was carried out using a well-calibrated portable handheld GQ GMC-500 Plus nuclear radiation detector at an elevation of about 1.0 m above ground level. A global positioning system from Garmin (GPSmap 78s) was adopted for identifying geographical locations. A total of 1172 points were surveyed across the study area for background environmental radiation. The BGR values ranged from 0.102 to 0.147 μSv/h, with an overall mean value of 0.126 μSv/h. The average measured dose rate was more than twice the reported world average value of 0.059 μSv/h. The annual effective dose equivalent (AEDE) for the research area was calculated to be 0.221 mSv/y on average. The mean AEDE is lower than the ICRP recommended limit. This shows that the population of the Minna area is radiologically safe based on the estimated AEDE value. Similarly, the excess lifetime cancer risk (ELCR) value ranged from 0.626 × 10−3 to 0.901 × 10−3 mSv/y with a mean value of 0.774 ± 0.09 × 10−3 mSv/y. The mean value of AEDE is below the 0.24 mSv/y permissible limits as recommended by the International Commission on Radiological Protection (ICRP). The mean ELCR value exceeds the average world value of 0.29 × 10−3. Also, the mean organ dose values estimated for the whole body, liver, kidney, testes, bone marrow, ovaries, and lungs are 0.150 ± 0.02, 0.102 ± 0.01, 0.137 ± 0.02, 0.181 ± 0.02, 0.152 ± 0.02, 0.128 ± 0.02 and 0.141 ± 0.02 mSv/y respectively. The differences in the calculated mean of BGR were attributed to natural and human factors. Geological variation is a fundamental factor that influences the changes in BGR. Human activities, mining, building materials, and the use of phosphate fertilizers in agricultural practices are responsible for the differences in BGR. The ELCR implies that terrestrial gamma radiation does not pose any immediate radiological health effects on residents of the area, but there is a tendency for long-term health hazards in the future, such as cancer, due to the dose accumulated.

Portable Wireless Handheld Ultrasound Linear Series UProbe-L5 and Hitachi Aloka F37 Ultrasound Reliability Test

Portable Wireless Handheld Ultrasound Linear Series UProbe-L5 and Hitachi Aloka F37 Ultrasound Reliability Test

Novel Sweat-Based Wearable Device for Advanced Monitoring of Athletic Physiological Biometrics

Blood testing has traditionally been the gold standard for the physiological analysis and monitoring of professional athletes. In recent years, blood testing has moved out of the laboratory thanks to portable handheld devices, such as lactate meters. However, despite its usefulness and widespread use, blood testing has several drawbacks and limitations, such as the need for the athlete to stop exercising for blood extraction and the inability to have data continuously collected. In this scenario, sweat has become an alternative to blood testing because of its rich content of electrolytes and metabolites, as well as small quantities of sugars, proteins, and ions. Nevertheless, there are few devices capable of analyzing this biofluid and providing useful information to users. In this paper, an electronic system designed for the autonomous analysis of sweat electrolytes and metabolites along with heart rate dynamics is presented. This system is part of a novel wearable device tailored for athletes that offers to the user a real-time assessment of their physiological status and performance.

2D vs. 3D Ultrasound Diagnosis of Pediatric Supracondylar Fractures.

Supracondylar fractures are common injuries in children. Diagnosis typically relies on radiography, which can involve long wait times in the ED, emits ionizing radiation, and can miss non-displaced fractures. Ultrasound (US) has the potential to be a safer, more convenient diagnostic tool, especially with new highly portable handheld 2D point of care US (POCUS). This study aimed to determine the reliability of 2D POCUS for the detection of supracondylar fractures and elbow joint effusions, to contrast the accuracy of 2D POCUS vs. 3DUS vs. radiographs, and to determine whether blinded image interpretation could produce similar results to non-blinded real-time imaging. Fifty-seven children were scanned with 2D POCUS and 3DUS on the affected elbow. US scans were then read by three blinded readers, and the results were compared to gold-standard radiographs. Compared to a gold standard of 30-day radiographic diagnosis, readers of 2D POCUS detected supracondylar fracture and effusion with sensitivities of 0.91 and 0.97, respectively, which were both higher than with 3DUS. Inter-rater reliability of fracture detection was moderate for 2D POCUS (k = 0.40) and 3DUS (k = 0.53). Consensus sensitivities, although high, were lower than reports from some non-blinded studies, indicating that clinical presentation serves as an important factor in detection rates. Our results from consensus US diagnosis support the validity of using 2D POCUS in children for supracondylar fracture and elbow effusion diagnosis.

Diabetic Retinopathy Screening Using a Portable Retinal Camera in Vanuatu.

Proof-of-concept study to test the feasibility of using an all-in-one portable retinal camera for the screening of diabetic retinopathy in the Pacific Island of Vanuatu, which has a high rate of diabetes and its associated complications and a dearth of ophthalmologists. From February 10, 2020, through February 28, 2020, 49 patients with diabetes mellitus from three islands in Vanuatu were recruited to participate in the study. Demographics, basic health data and retinal photography were obtained. A non-mydriatic, handheld camera was used (Volk Pictor Plus). Eleven participants (24%) had referral-warranted diabetic retinopathy. There was moderately high inter-rater reliability for our dependent variables: referral status (κ = 0.62, 95% CI 0.42-0.83), retinopathy severity (κ = 0.76, 95% CI 0.55-0.96), and clinically significant macular edema (κ = 0.50, 95% CI 0.25-0.74). Our study confirms that portable handheld cameras can be used to obtain retinal images of sufficient quality for diabetic retinopathy screening even in resource limited environments like Vanuatu. Among this cohort, a relatively high (24%) prevalence of referral-warranted diabetic retinopathy was found in Vanuatu.

Guided mode resonance immunosensor for label-free detection of pathogenic bacteria Pseudomonas aeruginosa

Photonic biosensors are promising platforms for the rapid detection of pathogens with the potential to replace conventional diagnostics based on microbiological culturing methods. Intricately designed sensing elements with robust architectures can offer highly sensitive detection at minimal development cost enabling rapid adoption in low-resource settings. In this work, an optical detection scheme is developed by structuring guided mode resonance (GMR) on a highly stable, transparent silicon nitride (SiN) substrate and further biofunctionalized to identify a specific bacteria Pseudomonas aeruginosa. The resonance condition of the GMR chip is optimized to have relatively high bulk sensitivity with a good quality factor. The biofunctionalization aims at oriented immobilization of specific antibodies to allow maximum bacteria attachment and improved specificity. The sensitivity of the assays is evaluated for clinically relevant concentrations ranging from 102 to 108 CFU/mL. From the calibration curves, the sensitivity of the chip is extracted as 0.134nm/Log10 [concentration], and the detection modality possesses a favorably good limit of detection (LOD) 89 CFU/mL. The use of antibodies as a biorecognition element complemented with a good figure of merit of GMR sensing element allows selective bacteria identification compared to other non-specific pathogenic bacteria that are relevant for testing physiological samples. Our developed GMR biosensor is low-cost, easy to handle, and readily transformable into a portable handheld detection modality for remote usage.

Improvement of Low-Cost Commercial Carbon Screen-Printed Electrodes Conductivities with Controlled Gold Reduction Towards Thiol Modification

Effectively detecting bacteria in the environment is crucial for researchers to make informed decisions about the safety of public areas, such as lakes. This led to an increased need in the development of portable handheld devices, capable of on-the-spot chemical and biological sensing applications. Specific interests lie in electrochemical biosensors and screen-printed electrodes (SPEs) due to the decreased costs, an ability to integrate with handheld devices, and their user-friendly nature. Together, these qualities make the devices more accessible in resource-poor settings. Two of the most common substrates used to fabricate SPEs are carbon and gold. Carbon SPEs are effective in sensing applications yet challenged when attempting to covalently attach biomolecules to the surface. Gold SPEs have higher affinity towards biomolecules and improve the sensitivity, selectivity, and stability of a device; yet they can be costly. A carbon SPE modified with gold may be an ideal candidate to create an efficient low-cost device, using electrochemical gold deposition. In this study, electrochemical gold deposition on SPEs is explored to enhance the surface area and conductivity towards sensing applications. These SPEs were then modified with a thiol-based self-assembled monolayer (SAM) which demonstrates this technique could be used for further modification towards biosensing.

Non-invasive imaging of pathological scars using a portable handheld two-photon microscope.

Pathological scars are a disorder that can lead to various cosmetic, psychological, and functional problems, and no effective assessment methods are currently available. Assessment and treatment of pathological scars are based on cutaneous manifestations. A two-photon microscope (TPM) with the potential for real-time non-invasive assessment may help determine the under-surface pathophysiological conditions in vivo . This study used a portable handheld TPM to image epidermal cells and dermal collagen structures in pathological scars and normal skin in vivo to evaluate the effectiveness of treatment in scar patients. Fifteen patients with pathological scars and three healthy controls were recruited. Imaging was performed using a portable handheld TPM. Five indexes were extracted from two dimensional (2D) and three dimensional (3D) perspectives, including collagen depth, dermo-epidermal junction (DEJ) contour ratio, thickness, orientation, and occupation (proportion of collagen fibers in the field of view) of collagen. Two depth-dependent indexes were computed through the 3D second harmonic generation image and three morphology-related indexes from the 2D images. We assessed index differences between scar and normal skin and changes before and after treatment. Pathological scars and normal skin differed markedly regarding the epidermal morphological structure and the spectral characteristics of collagen fibers. Five indexes were employed to distinguish between normal skin and scar tissue. Statistically significant differences were found in average depth ( t = 9.917, P <0.001), thickness ( t = 4.037, P <0.001), occupation ( t = 2.169, P <0.050), orientation of collagen ( t = 3.669, P <0.001), and the DEJ contour ratio ( t = 5.105, P <0.001). Use of portable handheld TPM can distinguish collagen from skin tissues; thus, it is more suitable for scar imaging than reflectance confocal microscopy. Thus, a TPM may be an auxiliary tool for scar treatment selection and assessing treatment efficacy.

Evaluating the Application of Portable Handheld X-ray Fluorescence (XRF) Scanner for Determining Seafood Provenance: A Case Study on Penaeus monodon.

Seafood elemental profiling (SEP) is the quantification of a range of elements in seafood products and may serve in addressing questions of seafood provenance and quality. Traditional methods for analyzing soft tissue present several limitations for the industry-level use of SEP. Portable handheld X-ray fluorescence (pXRF) analysis is a promising alternative to conventional methods; however, its application for biological analysis has not been fully established. Intact giant tiger prawn (Penaeus monodon) abdomens were analyzed with a Vanta M series XRF portable analyzer following a novel soft tissue protocol. Exploratory statistics (principal component analysis, nonmetric multidimensional scaling, and canonical discriminant analysis), as well as random forest models, have been implemented with pXRF profiles, yielding 81% accuracy when assigning the geographical origin of P. monodon. The results of this study highlight that SEP via pXRF is a viable industry-level analysis, and its application will depend on improved instrument calibration to account for fluctuating wetness factors that are influenced by cooking, storage, and other pre- and post-harvest treatments.

Portable manipulation system for commercial robotic surgery forceps.

Transition from the utilisation of traditional instruments to new robotic methodologies in surgical operations occurs rapidly. Although the implementation of these methodologies to classical surgery operations is advantageous due to increased precisions and enhanced motion capabilities of robotic systems, overall worldwide accessibility and adaptation are still limited due to high equipment costs and special infrastructure requirements. The design of four degrees of freedom low cost, compact and portable manipulation system was proposed to allow the utilisation of commercial robotic surgery forceps like a conventional laparoscopy instrument without the necessity of bulky manipulation systems. The structural design of the system was carried out along with necessary kinematic and coupled motion analysis. Prototypes were constructed. Hardware verification of the system was executed using implemented control methodology. The proposed design successfully demonstrated the advantages of multi degree of freedom robotic surgery forceps in a portable handheld system compared to classical laparoscopy instruments.

In-Field Obsidian XRF Analysis of Sites in the Lion Mountain Area and Gallinas Mountains of West-Central New Mexico

ABSTRACT The Gallinas Mountains of west-central New Mexico were home to agriculturalists between ca. a.d. 850 and 1450. Ongoing research of the Lion Mountain Archaeology Project (LMAP) has allowed for in-field analysis of surface obsidian artifacts via portable handheld energy dispersive X-ray fluorescence spectrometry (ED-XRF). Influenced by principles of preservation archaeology and the archaeological repository curation crisis, the LMAP has sought to design and conduct field research to gather the most data with the least amount of impact to sites. Non-destructive in-field analysis via portable handheld ED-XRF subscribes to these goals while also providing answers to significant questions about human behavior. Results show that variable patterns of obsidian source utilization and procurement strategies existed both through time and between contemporaneous populations. These results are indicative of a shifting system of exchange and social interaction between residents of the region and surrounding areas during the Ancestral Pueblo occupation of the area.

Miniaturized Vis–NIR handheld spectrometer for non-invasive pigment quantification in agritech applications

Advanced precision agriculture requires the objective measurement of the structural and functional properties of plants. Biochemical profiles in leaves can differ depending on plant growing conditions. By quantitatively detecting these changes, farm production processes can be optimized to achieve high-yield, high-quality, and nutrient dense agricultural products. To enable the rapid and non-destructive detection on site, this study demonstrates the development of a new custom-designed portable handheld Vis–NIR spectrometer that collects leaf reflectance spectra, wirelessly transfers the spectral data through Bluetooth, and provides both raw spectral data and processed information. The spectrometer has two preprogramed methods: anthocyanin and chlorophyll quantification. Anthocyanin content of red and green lettuce estimated with the new spectrometer showed an excellent correlation coefficient of 0.84 with those determined by a destructive gold standard biochemical method. The differences in chlorophyll content were measured using leaf senescence as a case study. Chlorophyll Index calculated with the handheld spectrometer gradually decreased with leaf age as chlorophyll degrades during the process of senescence. The estimated chlorophyll values were highly correlated with those obtained from a commercial fluorescence-based chlorophyll meter with a correlation coefficient of 0.77. The developed portable handheld Vis–NIR spectrometer could be a simple, cost-effective, and easy to operate tool that can be used to non-invasively monitor plant pigment and nutrient content efficiently.

Portable Handheld "SkinPen" Loaded with Biomaterial Ink for In Situ Wound Healing.

In situ bioprinting has emerged as an attractive tool for directly depositing therapy ink at the defective area to adapt to the irregular wound shape. However, traditional bioprinting exhibits an obvious limitation in terms of an unsatisfactory bioadhesive effect. Here, a portable handheld bioprinter loaded with biomaterial ink is designed and named "SkinPen". Gelatin methacrylate (GelMA) and Cu-containing bioactive glass nanoparticles (Cu-BGn) serve as the main components to form the hydrogel ink, which displays excellent biocompatibility and antibacterial and angiogenic properties. More importantly, by introducing ultrasound and ultraviolet in a sequential programmed manner, the SkinPen achieves in situ instant gelation and amplified (more than threefold) bioadhesive shear strength. It is suggested that ultrasound-induced cavitation and the resulting topological entanglement contribute to the enhanced bioadhesive performance together. Combining the ultrasound-enhanced bioadhesion with the curative role of the hydrogel, the SkinPen shows a satisfactory wound-healing effect in diabetic rats. Given the detachable property of the SkinPen, the whole device can be put in a first-aid kit. Therefore, the application scenarios can be expanded to many kinds of accidents. Overall, this work presents a portable handheld SkinPen that might provide a facile but effective approach for clinical wound management.