Port System Research Articles

First experience of distal hybrid surgery on lower limb arteries with port-system installation

Cases of occlusive lesions of the arteries of the lower leg occupy a special place in the treatment of critical lower limb ischemia. In this regard, performing open reconstructive interventions is unpromising. A new era in the treatment of occlusions of the arteries of the lower leg has become endovascular surgery. However, due to extensive occlusive lesions of the arteries of the lower leg, direct endovascular revascularization is often impossible. Currently, vascular surgeons adhere to the angiosome principle, according to which the goal of balloon angioplasty is to restore blood supply to the artery supplying the angiosome with purulent-trophic changes. In the clinical example provided, the universality of the angiosome theory is questioned, and clinical success achieved due to the discovery of collateral blood fl ow is demonstrated. The patient underwent surgery with the use of an original hybrid operation technique with the installation of a port system followed by active therapeutic-diagnostic measures on an outpatient basis for 30 days. As a result of the treatment, epithelialization of the trophic defect and cessation of critical ischemic phenomena were noted.

Syringe Port System as a Tubular Retractor Technique for Brain Lesions: Case Series and Review of the Literature

Abstract Objective To describe a tubular retractor technique for brain lesions through a series of cases and to conduct a literature review on intracranial tubular retractors with emphasis on the syringe port system. Materials and Methods We described four cases of lesions with an intraventricular component and different pathological patterns. The surgeries were performed between April, 2021 and July, 2022. The images were acquired through computed tomography (CT) and magnetic resonance imaging (MRI) scans and transferred to the Horos software, version 1.1.7. To make the tubular retractor, a 20-mL syringe and a 14-Fr/Ch, 30-mL/cc Foley probe were used. The syringe was sectioned according to the planned depth based on preoperative imaging. The syringe was the retractor itself, while the probe served as a means of dilating the path to the lesion. Results Gross total resection was achieved in all cases, and the samples collected were satisfactory regarding the results of the anatomopathological study. All patients evolved without any additional deficits and with adequate postoperative image control. Conclusion The syringe as a tubular retractor associated with the Foley probe as a surgical port dilator was useful, and it enabled the radical resection of intracranial tumors related to the lateral ventricle, not limiting the use of auxiliary instruments, neither of microsurgical instruments; therefore, it is an affordable, secure, and inexpensive method.

Factor analysis method for delay dynamics in Nigerian Tin Can Island Port for sustainable development

The effect of efficient and effective port system on the economy viability of any nation cannot be over emphasis. This study investigated factors that could be termed as delay causative factors in Nigerian ports industry using TIN CAN Island port complex as a case study. A well-structured questionnaire was developed for this research and 72 respondents, who are port users (custom officers, employees, and customers), were asked to rank the variables perceived to be causing delay at the port according to their respective perception of the effect of each factor on delay factors at the port. The validity of the research instrument was done using Cronbach Alpha. There are several factors that were thought to be working together in reducing efficiency in Nigerian ports and these factors were analyzed using factor analysis method. It was discovered from the analysis that Lack of Government Authority, Ullage Constraint, Inadequate Berthing, Logistics Delay, Financial Constraint and Equipment Breakdown are major factors that cause delay at Tin Can Island Port Lagos. These highlighted factors should be paid special attention in order to reduce delay in Tin Can Island port which will in turns lead to more revenue for the country.

Metaverse in ports through an affinity diagram

A metaverse is an environment where humans interact socially and economically as avatars in cyberspace, which acts as a metaphor for the real world but without its physical or economic limitations. Many people use this new technology to connect with others, exchange content or discover new hobbies. Unlike other virtual worlds, the metaverse offers an online world that can be shaped. For the ports of the Spanish port system, it is intended to determine the new virtual port ecosystem that could be developed in the short term through an affinity diagram, which is a diagram that is used for the organization of ideas provided by a group on a complex problem that is held in a specific area, in this case reaching meta ports in the port system. The main conclusion is that to advance on this concept the new operating models and customers and services, are the blocks where the greatest efforts will have to be made.

Subcostal bilateral internal thoracic artery harvesting with the da Vinci Single Port system: an experimental feasibility study.

We evaluated the feasibility of harvesting bilateral internal thoracic arteries with the da Vinci Single Port system (SP)through a single left-sided subcostal incision. Complete bilateral mobilization with sufficiently long conduits for multivessel grafting was possible in 2 human cadavers and 2 live porcine. Creating the subcostal access and docking the SP system took between 14 and 21 min and the total harvest time ranged from 65 to 125 min in all models. No major bleeding was observed in the live porcine and hemostasis was managed with the available instrumentation. One porcine deceased during surgery due to ventricular fibrillation followed by cardiac arrest. The robotic harvesting was technically easily reproduced by the surgeons and required no additional rib-spreading. Further studies will be required to assess if this subcostal approach with the da Vinci SP system yields true clinical benefits in patients.

Totally implantable venous ports in infants and children: a single-center retrospective study of indications and safety.

Totally Implantable Venous Access Devices (TIVADs) contribute significantly to the treatment progress and comfort of patients requiring long-term therapy. However, the procedure for implanting TIVADs, as well as its very presence, may be associated with complications. This study evaluates the indications, safety, and complication rates of venous port implantations in pediatric patients. It also explores factors influencing the occurrence of early and late complications post-implantation. The study included 383 pediatric patients treated at the Department of Pediatric Surgery, Traumatology, and Urology in Poznan between 2013 and 2020 who underwent 474 implantations of intravenous ports. Venous access was achieved using the Seldinger technique. Statistical analysis was performed using Statistica 13 with TIBCO and PQStat 1.8.2.156 with PQStat. Venous ports were used in 345 oncology patients requiring chemotherapy (90% of the total group) and in 38 children (10%) with non-oncology indications. There were 36 early complications (7.6%) and 18 late complications (3.8%), excluding infectious complications. The most common early, non-infectious complications included pneumothorax (15 patients; 3%) and port pocket hematoma (12 patients; 2.5%). The most common late, non-infectious complications observed were venous catheter obstruction (8 children; 1.7%) and port system leakage (5 children; 1%). Infectious complications occurred in 129 cases (27.2%). Children with a diagnosis of non-Hodgkin's lymphoma, acute myeloid leukemia, and acute lymphoblastic leukemia had a significantly higher incidence of port infections. Venous ports equipped with a polyurethane catheter, compared to systems with a silicone catheter, functioned significantly shorter. The Seldinger method of port implantation is quick, minimally invasive, and safe. The type of port, including the material of the port's venous catheter, and the underlying disease have an impact on the durability of implantable intravenous systems. The experience of the surgeon is related to the frequency of complications associated with the procedure.

The Port Sector in Italy: Its Keystones for Energy-Efficient Growth

Italy has been defined as the “logistics platform” of the Mediterranean Sea. The Italian port system, with 11.6 million TEUs handled and 61.4 million passengers in 2022 (Assoporti data January–December 2022), is the key to fulfilling this title through adequate levels of reliability, safety, and sustainability. This contribution addresses port logistics and shipping, focusing on primary issues related to the energy sector with a specific focus on what can be observed in the Italian context. Specifically, the decarbonization of the maritime sector and related infrastructural problems (e.g., cold ironing or alternative fuels, where the uncertainty about resource availability and related costs do not allow for easy strategic planning by both the ship owner and the port authority), as well as policies such as the Emission Trading System (ETS), will be analyzed. All these issues, hereafter addressed with a systematic critical review of the existing literature and other relevant sources, could represent the driving force of the growth of the national port sector toward its competitiveness at a global scale. The review was performed through a wide search and analysis of studies published in well-known online research databases (Scopus, Web of Science, IEEE Xplore, ScienceDirect), sector studies, or specialized technical magazines. The review focuses on the results of each analyzed contribution rather than on the analysis method adopted with the final aim to identify useful hints and innovative ideas for further studies on the topic.

Differences in the Incidence of Sterile Inflammation After Trabectedin Infusion With Two Central Venous Port Systems: A Retrospective Study.

Purpose Sterile inflammation along the tunneled catheter is a characteristic complication associated with trabectedin infusion via a central venous port (CVP). To date, no studies have evaluated the differences in sterile inflammation incidence according to the CVP system used. This study evaluated the differences in sterile inflammation incidence between two different CVP systems. Methods This study was conducted at The University of Tokyo Hospital, Bunkyo-Ku, Tokyo, Japan. Patients with trabectedin infusion using CVP via the internal jugular vein between April 2016 and February 2024 were retrospectively evaluated. Sterile inflammation was characterized as skin erythema, swelling, pain, or induration along the tunneled catheter after infusion of trabectedin from the CVP and negative for various infection tests. The incidence of sterile inflammation was compared using two different CVP systems: Anthron® polyurethane catheter with Celsite port (P-U Celsite; Toray Medical, Tokyo, Japan) and DewX Eterna (Terumo, Tokyo, Japan). Results Of the 21 patients, 12 and nine patients used P-U Celsite and DewX Eterna for trabectedin infusion, respectively. Sterile inflammation occurred in five patients; of these, four underwent CVP removal because of worsened pain, making trabectedin infusion difficult. Sterile inflammation occurred in 0 (0/12) and 56% (5/9) of patients using P-U Celsite and DewX Eterna, respectively, with a significantly lower incidence in patients using P-U Celsite (P = 0.006). Conclusion Sterile inflammation incidence was significantly lower in patients using P-U Celsite compared to those using DewX Eterna.

Effects of palliative intrathecal analgesia on patients with refractory cancer bone pain.

This study examined the effects of intrathecal analgesia (ITA) using an extracorporeal pump with a subcutaneous port system in cancer patients with bone metastasis. Among the patients who died of cancer with bone metastasis at the palliative care unit of our institution, 11 who received ITA were selected. Changes in pain, opioid doses, the palliative prognostic index (PPI), and Eastern Cooperative Oncology Group Performance Scale after ITA were assessed. Pain, opioid doses, and PPI decreased after ITA (P = 0.002, 0.002, and 0.017). ITA for cancer patients with increased PPI due to refractory cancer bone pain decreased pain, opioid doses, and PPI.(100 words).

Assessment of Consistency in the Functioning of Seaports of Ukraine

Introduction. Seaports in a state’s economies are the main levers of economic development, investment generators, and innovation demand. Modern trade ports are open systems that are influenced by many factors. During the last decades of globalization and innovative technologies, an essential factor in the competitiveness of seaports, such as the stability of the functioning of Ukraine, is gaining relevance. The improvement of port management is driven by the urgent need to apply innovative strategies and methods to ensure the development and efficiency of the port industry due to the impact of many factors affecting the stability and growth of the port system.
 Aim and tasks. The aim of the study is to assess the influence of various factors on the consistency of the management system of the port industry.
 Result. An algorithm for forming generalized stability indicators for the functioning system of the port industry is proposed. Using an entropy approach to the management indicators of the development of the port industry functioning system showed an increase in the indicators of the ship operations group by 1.07% and cargo operations by 1.01%, a decrease in the financial group by 1.65%, and a port environment by 1.30%. The application of the established approach and the defined sequence of relevant calculations will allow ports to respond sensitively to violations of the stability of their system both at the industry and individual port levels and to find answers for each group of selected influential indicators for the finance group, the ship and cargo operations group, and the port environment. The main difference between the proposed approach and the existing ones is the calculation of the overall level of functioning of the port management system, which is based on the accumulation of the values of the relevant integral indicators for groups of indicators such as finance, ship-cargo operations, and port environment.
 Conclusions. The operation system of the port economy can be considered from the perspective of researching its various states, which are a consequence of the dynamics of the external environment. This allows us to use such a quality of entropy by considering the final nature of the management processes of the industry. This will help determine the nature of trends that can be considered in the future when forecasting the port industry’s development directions and when making relevant comparisons in the industry at the national and global levels.

Potential of photon counting computed tomography derived spectral reconstructions to reduce beam-hardening artifacts in chest CT

PurposeAim of the recent study is to point out a method to optimize quality of CT scans in oncological patients with port systems. This study investigates the potential of photon counting computed tomography (PCCT) for reduction of beam hardening artifacts caused by port-implants in chest imaging by means of spectral reconstructions. MethodIn this retrospective single-center study, 8 ROIs for 19 spectral reconstructions (polyenergetic imaging, monoenergetic reconstructions from 40 to 190 keV as well as iodine maps and virtual non contrast (VNC)) of 49 patients with pectoral port systems undergoing PCCT of the chest for staging of oncologic disease were measured. Mean values and standard deviation (SD) Hounsfield unit measurements of port-chamber associated hypo- and hyperdense artifacts, bilateral muscles and vessels has been carried out. Also, a structured assessment of artifacts and imaging findings was performed by two radiologists. ResultsA significant association of keV with iodine contrast as well as artifact intensity was noted (all p < 0.001). In qualitative assessment, utilization of 120 keV monoenergetic reconstructions could reduce severe and pronounced artifacts completely, as compared to lower keV reconstructions (p < 0.001). Regarding imaging findings, no significant difference between monoenergetic reconstructions was noted (all p > 0.05). In cases with very high iodine concentrations in the subclavian vein, image distortions were noted at 40 keV images (p < 0.01). ConclusionsThe present study demonstrates that PCCT derived spectral reconstructions can be used in oncological imaging of the thorax to reduce port-derived beam-hardening artefacts. When evaluating image data sets within a staging, it can be particularly helpful to consider the 120 keV VMIs, in which the artefacts are comparatively low.

IPREDS: Efficient Prediction System for Internet-wide Port and Service Scanning

Internet-wide port and service scanning, a vital tool for network research, is unaffordable in time and network bandwidth consumption. However, scanning only a portion of ports and services may lead to erroneous research conclusions. Previous work has shortened scanning time by predicting potentially active ports and eliminating many invalid scan targets. Still, they suffer from inherent design flaws that compromise their performance in terms of prediction accuracy and efficiency. The vast, unevenly distributed, and noisy nature of active ports presents significant challenges for prediction systems. Meanwhile, service prediction work is still in a shortage state. In this work, we introduce IPREDS, the first efficient prediction system for Internet-wide port and service scanning. IPREDS uses its carefully designed decision model to utilize all input features and predict the scanning reward of each target in parallel, providing high coverage prediction results in minimal time. Our experiment results show that IPREDS can discover 87% of active ports across the entire IPv4 network within two hours, saving at least 87.26% of the total time and 59% of the packets sent compared to existing work. For service scanning, IPREDS finds 91% of all active services using only four handshakes on each active port and saves 85.9% time to find 69% of each active service compared to exhaustive service scanning.

Study on the hierarchical structure of container port systems along the South China Sea in the context of low carbon emissions

Under the low-carbon background, 37 ports of countries along the South China Sea with capacity ranking in the world’s top 100 container ports are selected as the research object to establish a shipping network, construct a directed dichotomous shipping network, and divide the port tiers after finding the kernel number by using kernel analysis method, respectively, to explore the relationship between tiers and the relationship between ports at the higher level, and then make clear the direction of the improvement for the reduction of carbon emissions. The results show that: the routes sent between container ports in countries along the South China Sea have formed a more stable connection, but the receiving routes are still in a passive position, and the port call relationship is affected by the port hierarchy. High-level ports such as Shanghai Port and Singapore Port undertake the transshipment function of cargo concentration and redistribution within the network. The small-world characteristics among ports such as Singapore Port, Ningbo Port, Haiphong Port, Shenzhen Port, Guangzhou Port, and Linchaban Port are obvious. Based on the results of the empirical analysis, corresponding suggestions are put forward to optimize the structure of the container port system in the countries along the South China Sea and promote the low-carbon development of the sea.

Removal of the central venous port system in children. Is this an appropriate training procedure for resident physicians? A single-center retrospective study.

&lt;b&gt;Introduction:&lt;/b&gt; A venous port system consists of a port chamber attached to a central catheter, which is implanted into the central venous system. The removal of the vascular port includes all items of this system. This procedure is usually simple and quick. Complications with port removal are rare but possible.&lt;b&gt;Aim:&lt;/b&gt; The study aimed to summarize venous port removal procedures performed by pediatric surgeons.&lt;b&gt;Material and methods:&lt;/b&gt; The single-center, retrospective study was conducted on 360 pediatric patients treated during the years 2010-2022 in the Department of Pediatric Surgery, Traumatology, and Urology in Poznan.&lt;b&gt;Results:&lt;/b&gt; The port usage time before the removal ranged from 3 days to 8 years and 3 months, with an average of 22 months. The leading cause of vascular port removal was the end of treatment (78.06%). Other less frequent reasons were the infection (13.89%) and mechanical damage of the port (3.33%). There were almost no complications connected with the procedure of vascular port removal (92.2%). If the complications occurred, they included bleeding during surgery (3%), covering the vascular catheter with bone tissue of the clavicle, causing difficulties with the catheter removal from the vein (1.4%), and connected with the previous complications-prolonged clavicle pain after surgery (1.4%) and hematoma after surgery (2%).&lt;b&gt;Conclusions:&lt;/b&gt; Removing the venous port is a safe procedure with a low risk of complications. This procedure can be considered as an appropriate training procedure for young trainees. A more extended period of use of the vascular port before its removal may be associated with more difficulties during surgery.

Influence of a changing wave climate on the quality and morphometry of the stalked barnacle Pollicipes pollicipes (Gmelin, 1789), along the coasts of NW Iberia

Wave climate is shifting over the last decades along the Atlantic coasts of Europe ultimately driven by large-scale patterns of atmospheric variability forced by anthropogenic global warming. Changes in wave height and surf zone orbital currents are hypothesized to drive marked shifts in the shape of intertidal organisms such as the stalked barnacle Pollicipes pollicipes, whose quality and market price are known to decrease non linearly with the peduncle length: width ratio S. This study evaluates wave trends in NW Iberian Peninsula, using the Spanish Port System 2006–2020 SIMAR wave hindcast. On the other hand, trends in stalked barnacle morphology and quality are estimated from 26 sites at the management regions of Baiona and A Guarda between 2011 and 2020. Results show evidence of temporal changes in barnacle quality and, especially, morphometry caused by simultaneous shifts in winter wave induced orbital currents. Because of the non linear relationship between S and the high quality threshold, large increases in S are usually translated to small reductions in quality. However, we identified a tipping point around S = 2.4 that if surpassed can lead to great drops in barnacle quality. In addition, changes in wave forcing will have different effects at each extraction site, as trends in wave climate are decoupled from barnacle morphometry at steeper sites sheltered from the predominant wave direction. In conclusion, this knowledge could be applied to develop site specific barnacle harvesting strategies based on annual wave climate forecasts.Graphical abstract

Initial experience and surgical outcomes of robotic-assisted total hysterectomy using the da Vinci SP surgical system.

The da Vinci SP surgical system is a surgical platform capable of implementing robotic-assisted surgery through a single port and was first introduced in Japan at our hospital. In this paper, we describe our experience of the initial introduction of the da Vinci SP surgical system and its surgical outcomes. This is the first report on the surgical outcomes of using da Vinci SP, and its comparison with the conventional system in Japan. After developing an application for a highly difficult new medical technology in-house, we compared the surgical outcomes (median values) of 15 patients who had undergone total hysterectomy at our hospital using the da Vinci SP (1-port) system (SP group) for uterine myoma after March 2023 and of 154 patients who underwent total hysterectomy using the conventional da Vinci Xi (four ports) system (Xi group) for uteri weighing <500 g. The results of the comparison of the characteristics between 15 patients in the SP group and 154 patients in the Xi group were as follows: uterus weight (g): 230 (90-500) versus 222 (55-496) (p = .35); surgical time (minutes): 199 (171-251) versus 198 (88-387) (p = .63); intraoperative blood loss (mL): 13 (5-82) versus 20 (2-384) (p = .17); and rate of surgical complication (%): 0.0 versus 1.3 (p = .66). The data indicated a comparable weight of the resected uterus, surgical time, intraoperative blood loss, and rate of surgical complications between the two groups. Robotic-assisted total hysterectomy using the da Vinci SP surgical system allowed clinicians to safely perform surgeries according to the conventional systems.

The effects of the COVID-19 pandemic on connectivity, operational efficiency, and resilience of major container ports in Southeast Asia

The COVID-19 pandemic in 2020 has had significant impacts on the connectivity and performance of container ports in Southeast Asia. The application of social network analysis (SNA) in conjunction with key indicators, such as container throughput, total capacity of ships, number of ship calls, and berth duration, provides a comprehensive understanding of the development of the regional port system. While major ports in the region, namely Singapore, Port Kelang, Manila, and Laem Chabang, have exhibited signs of recovery owing to the expeditious and timely implementation of targeted policies to mitigate the adverse effects of the pandemic, smaller ports in the region, such as Bangkok, Penang, Tanjung Priok, and Tanjung Perak, are grappling with enduring repercussions and encountering challenges in resilience. These challenges stem from inadequate infrastructure, limited accommodation capacity, low loading/unloading productivity, and other significant risk events associated with the pandemic, all of which have weakened their competitiveness. In contrast, ports in Vietnam, including Cai Mep, Hai Phong, and Ho Chi Minh, as well as Malaysia's Tanjung Pelepas, have demonstrated that they are less impacted by the COVID-19 pandemic. This can be attributed to the sustained growth of their respective economies and the continued surge in domestic production to meet the unabated demand for exports. Based on these findings, several policy recommendations can be suggested for port authorities to effectively manage and mitigate the impact of risky events, such as pandemics, in the future while also enhancing the competitiveness of ports.

A REVIEW ON DEVELOPMENT OF COLON TARGETED DRUG DELIVERY SYSTEM

The purpose of this review was to select a promising drug delivery system for colon diseases. This review covers the development of Colon Targeted Drug Delivery System (CTDDS) using 36 y (1986-2022) data from various research and review articles. All fig. designed using by BioRender website. vThe colon-targeted drug delivery systems developed for the specific site drug delivery which applied for both local and systemic actions of the drug; since the drug targeted to be release within the colon, the unwanted systemic side effects are reduced along with it. Systemic side effects include organ damage, respiratory diseases and, cardiovascular damage and other illnesses. Colon-targeted drug delivery system used in the treatment of diseases in the colon, including ulcerative colitis, irritable bowel syndrome and colorectal cancer. The benefit of colon-targeted drug delivery besides the reduction of side effects also include protection from premature drug release or burst in the stomach or small intestine before reaching the colon. For the development of drugs with such benefits and advantages, drug delivery systems and approaches have used for Colon targeted drug delivery systems, varying from conventional colon-targeting drug delivery systems to novel approaches for Colon-targeted drug delivery systems. Conventional drug delivery includes the use of prodrugs, pH-dependent, time-dependent, matrix-based systems, polysaccharides-derived systems, and bio-adhesive system while novel approaches include types such as port system, pulsincap system, pressure-controlled system, osmotic controlled system, CODES, and the newest approach wish is the use of nanotechnology in colon targeted drug delivery. In this research both techniques reviewed, and their types discussed as well. The limitation of their uses and the advantage of each system discussed with a breakdown of the different mechanisms used to formulate such systems. A successful colon targeting delivery can release the drug to a specific segment in colon due to presence of different colonic enzymes formed by microorganisms that metabolize drug carrier linkage. Use of combined approaches i.e., conventional systems and newer approaches may be the best way to cure colon diseases using an optimized colon drug delivery system.

Final design analysis of the optical hinge of the wide angle viewing system for ITER equatorial port 12

The ITER Visible/Infrared Wide Angle Viewing System (WAVS) is a machine protection optical diagnostic that is being developed for ITER. The first two ex-vessel components of WAVS are the Optical Hinge (OH) and Optical Relay Unit (ORU) and both share a common support structure. Equatorial Port 12 (EP12) WAVS should be operational for the first plasma and its facing its Final Design Review (FDR). It is being developed by the Consortium constituted by CEA, CIEMAT, INTA and Bertin Technologies through a Grant Agreement financed by F4E.To compensate vacuum vessel vertical displacements respect to the building during operation, an Optical Hinge (OH) will be placed at the beginning of the ex-vessel area for each Line of Sight (LoS). Each OH is formed by a pair of mirrors one of which is moveable using a piezoactuator. The OHs are attached to the common support which is, in turn, fixed to the Interspace Support Structure.To assure the optical performance, the common support structure has to withstand operational loads with low deviation and tilt. In addition, the structural integrity of the common structure has to be maintained in case of seismic or accidental loads as it is classified as a Safety Relevant component.This work exposes the analyses performed to face the FDR of ex-vessel WAVS in EP12. It comprises the structural analyses performed by CIEMAT for the OHORU common support structure to assure its integrity according to RCC-MR Code and the detailed model of one OH LoS to check also the diagnostic optical requirements.

A technique for repeated blood and cerebrospinal fluid sampling from individual rats over time without the need for repeated anesthesia

Ethical animal use follows the 3R’s: Replacement, Reduction and Refinement. Here, we present the use of simultaneous jugular vein and cisterna magna catheterization via a port system in rats for repeated fluid sampling for 14 consecutive days without loss of catheter patency. This technique allows repeated intra-animal sampling without anesthesia and, if used with pooling samples from a cohort of animals, replaces the need for terminal collections for sufficient sample volumes.