An experimental investigation on the spatial distribution of knock events in a turbocharged spark-ignition engine for hybrid vehicle applications was conducted by using a multichannel fiber optic method. The knock positions were detected under different conditions to investigate the influence of crucial engine design and operating parameters on the knock characteristics including the spatial distribution in the combustion chamber and its relationship to knock intensity. The measured data reveal that the spatial distribution in the engine with a port fuel injection (PFI) system is mainly located on the exhaust side with insignificant influence of engine speed and load, which is attributed to the elevated thermal load around the exhaust valves. However, the knock events under gasoline direct-injection (DI) conditions were found to occur in more scattered locations with more occurring on the engine front-end and rear-end sides. These results indicate that the in-cylinder fuel-air mixing process may have a significant impact on the knock occurrence spots under DI conditions. The knock positions of the engine with different excessive air ratios, injection timings, and intake-valve timings were also detected, indicating that engine operating parameters have complex influences on the knock-region distribution in a DI engine. In addition, experiments were also carried out in two different cylinders to verify the cylinder-to-cylinder variations in knock regions which may be caused by the engine cooling design. Furthermore, no apparent correlations were observed between the knock position and the knock intensity by analysis of the experimental data.