Porous Polymer Gels Research Articles

Studies on Chemical Analysis of Mycotoxin (VI)

The high performance liquid chromatographical determination of penicillic acid and patulin was carried out by means of the combination of conventional pump, glass column packed with porous polymer gel (Iatrobeads 6cp-2010), and UV-detector. Mycotoxins were extracted, as reported previously, with CH3CN-4%KCl-20%H2SO4 (89:10:1) and transfered into CHCl3 layer, which was subsequently applied to colum chromatography of silicic acid. The fraction eluted with CHCl3-methanol (97:3) contained penicillic acid and patulin, which were then freed from solvent and dissolved with small amount of methanol.An aliquot of the methanol solution was employed as a sample for liquid chromatography. Penicillic acid and patulin were eluted in good separation each other with methanol-H2O (9:1) at a moving rate of 0.7ml/min. UV-folw detector system was operated at 230nm. Minimun detectable amounts giving about 1.5cm peak height on chromatogram of Penicillic acid and Patulin were 50 and 102ng, respectively. Therefore, detection limits of Penicillic acid and Patulin in food sample become as low as 5 and 10ppb, respectvely.