Porous Cryogels Research Articles

Highly porous cryogels loaded with bimetallic nanoparticles as an efficient antimicrobial agent and catalyst for rapid reduction of water-soluble organic contaminants

The development of robust materials possessing simultaneously antibacterial and catalytic activities for the reduction and removal of organic pollutants from wastewater with enhanced efficacy has received unwavering interest. Herein, highly porous and thermo-responsive cryogels of poly(N-isopropylacrylamide-co-acryamido-2-methylpropane sulfonic acid) have been fabricated via redox-initiated cryo-polymerization. Silver/gold bimetallic nanoparticles were introduced into a bare cryogel through thermal reduction of metal salts. The fabricated bare cryogels and bimetallic integrated cryogel were confirmed with different techniques including fourier transform infrared spectroscopy. The crystallinity of bare cryogels and bimetallic nanoparticles inside cryogel was examined by using powder X-ray diffraction. Thermo-gravimetric analysis confirmed that bimetallic nanoparticle loaded cryogels were thermally more stable than bare cryogels. The macro-porosity of cryogels before and after loading bimetallic nanoparticles were studied by using scanning electron microscopy. Energy dispersive X-ray and X-ray photoelectron spectroscopy were implemented, confirming the presence of metal elements in the synthesized bimetallic hybrid cryogel. Furthermore, the uniform distribution of silver and gold nanoparticles in cryogel was examined with transmission electron microscopy. The fabricated bimetallic nanoparticle loaded cryogel exhibited high antibacterial activities against E. coli and S. aureus as well as the good catalytic capacity for the rapid reduction of p-nitrophenol and rhodamine B. Catalytic activity was also examined at various temperatures, different pH values and catalyst amounts. The activation energy (Ea = 34.41 kJ mol-1), enthalpy change (ΔH = - 31.92 kJ mol-1) and entropy change (ΔS = −145.19 J mol-1 k-1) of catalytic reduction activation were determined using Arrhenius and Eyring equations. The catalytic performance of bimetallic nanoparticle loaded cryogel was also examined in pure water and tap water. The fabricated bimetallic catalyst was highly stable up to five consecutive cycles without any change in their catalytic performance.

Synergetic effects of water-soluble polysaccharides for intensifying performances of oleogels fabricated by oil-absorbing cryogels

Oleogels were prepared by the cryogel-templated method from porous cryogels, which were co-structured using hydroxypropyl-methylcellulose and structural enhancers (such as flaxseed gum, κ-carrageenan, carboxymethyl-cellulose, arabic gum, and guar gum). The hardness, network density, pore size, pore volume, and SEM micrographs of cryogels showed that κ-carrageenan and flaxseed gum could keep the integrity of aqueous foams during freeze-drying and endow cryogels with the high hardness and content to hold oils (>98%). Oil absorption curves indicated that flaxseed gum and guar gum-enhanced cryogels provided the fastest oil absorption rate due to bigger pores. The absorption model was fitted well with all experimental data. Physical and mechanical properties of cryogels were positively related to the rheological property and oil bonding capacity of oleogels. κ-carrageenan and flaxseed gum were more suitable as structural enhancers to improve hydroxypropyl-methylcellulose-based cryogels for preparing oleogels to replace plastic fats in foods.

Construction of foam-templated oleogels based on rice bran protein

In this study, it was discovered for the first time that oleogel can be prepared via a facile, low temperature process through rice bran protein (RBP) foam-templates. The RBP foams were freeze-dried to create a highly porous cryogel, which could physically absorb liquid oil and form an oleogel with strong mechanical strength. It was observed that both the foamability and foam stability of RBP were significantly enhanced when the pH was far from the isoelectric point (pH 4.6), which was mostly determined by change in the secondary structure and surface hydrophobicity of RBP. The foams prepared at higher pH (7.0, 9.0 and 11.0) showed excellent foaming capability. Scanning electric microscopy results showed that the corresponding cryogels exhibited dense and uniform porous networks with superior oil absorption ability (up to 18.39 g rice bran oil/g RBP cryogel). The foam prepared at pH 3.0 showed a particularly high foam stability, due to formation of a strong interfacial film filled with tiny bubbles. However, the corresponding cryogel was less efficient in oil absorption due to less porous structure after freeze dried. The resulting oleogels showed high G′ (up to 1.5 × 105 Pa) and yield stress (up to about 1000 Pa), indicating a strong interaction between the cryogel and rice bran oil. The gel strength of oleogel was inversely correlated to oil absorption rate of the corresponding cryogels. This work provides a novel route for fabrication of foam-templated oleogels with RBP as the sole structurant, which were great candidates for hard-stock fat replacement with zero trans fatty acid and low saturated fatty acids.

A dumbbell-shaped stir bar made from poly(3,4-ethylenedioxythiophene)-coated porous cryogel incorporating metal organic frameworks for the extraction of synthetic phenolic antioxidants in foodstuffs

A dumbbell-shaped stir bar adsorbent of MIL-101 entrapped in PVA cryogel coated with poly(3,4-ethylenedioxythiophene) was fabricated to extract synthetic phenolic antioxidants in foodstuffs. The interconnected porous of cryogel allowed the entrapment of MIL-101 and enhanced the surface areas of poly(3,4-ethylenedioxythiophene) coating which facilitated multiple adsorptions. The fabricated adsorbent was characterized and measured the adsorption capacities for synthetic phenolic antioxidants. Extraction efficiency was optimized by evaluating the effect of adsorbent compositions, extraction time, stirring speed, sample pH, desorption conditions, sample volume and ionic strength. The analysis of extracted synthetic phenolic antioxidants was carried out using high performance liquid chromatography. The developed analysis method provided a wide linear range of 0.20 - 200 µg kg−1 for butylated hydroxyanisole and 0.50 - 200 µg kg−1 for tert‑butylhydroquinone and butylated hydroxytoluene. The limits of detection were between 0.05 and 0.15 µg kg−1. The developed stir bar adsorbent was utilized to extract these three synthetic phenolic antioxidants from juice, milk, infant formula and coffee creamer. Recoveries ranged from 87 to 101% with RSDs below 7%. The developed composite stir bar adsorbent was convenient to use, and good physical and chemical stability allowed efficient extraction for 12 extraction cycles.

High-strength anti-bacterial composite cryogel for lethal noncompressible hemorrhage hemostasis: Synergistic physical hemostasis and chemical hemostasis

Porous cryogel with its poor mechanical properties greatly limit its application for lethal non-compressible bleeding from deep wounds. Here, we creatively combined foaming reaction and cryo-polymerization reaction to prepare a series of high-strength composite cryogel hemostatic agents based on Poly(vinyl alcohol) (PVA), Carboxymethyl chitosan (CMCS) and Dopamine (DA) to cope with lethal noncompressible bleeding. The optimized PVA/CMCS-DA6 cryogel (1 mL prepolymer solution contains 6 mg DA) exhibited compression stress higher than 125 kPa at 80% compression strain and tensile stress-at-break higher than 155 kPa which are much higher than that of traditional cryogels. The hemostatic material with shape memory behavior and high compression strength can effectively stop lethal non-compressible bleeding, which has a good physical hemostatic effect, and the hemostat is easy to be removed after hemostasis. In addition, PVA/CMCS-DA6 cryogel showed better whole blood clotting ability, platelets and blood cells activation ability than commercial gelatin sponge and medical gauze, which has a good chemical hemostatic effect. PVA/CMCS-DA6 cryogel with synergistic chemical hemostasis and physical hemostasis showed outstanding hemostatic capacity in the rabbit liver defect non-compressible hemorrhage model and lethal coagulopathic rabbit liver defect bleeding model. Therefore, high-strength PVA/CMCS-DA cryogels have great potential as a new hemostatic agent for controlling lethal bleeding and providing a new idea for the design of efficient hemostatic materials.

Multifunctional 3D cationic starch/nanofibrillated cellulose/silver nanoparticles nanocomposite cryogel: Synthesis, adsorption, and antibacterial characteristics

We report a new 3D nanocomposite cryogel combines the advantages of cationic starch (Cs), nanofibrillated cellulose (NFC) and silver nanoparticles (Ag NPs). Cs was the main component of the cryogel while NFC was used as a filling agent to enhance the mechanical properties of the produced cryogel. Both Cs and NFC endow the cryogel with adsorption properties while Ag NPs enhances its antibacterial properties. Ag NPs was green synthesized with the aid of microwave radiation using NFC as reducing and stabilizing agent. The prepared Ag particles were free of impurities with sizes <10 nm and good stability in solution. Two different concentrations of the prepared Ag NPs were added to a mixture of Cs and NFC and subjected to freeze drying to get porous cryogel (3D microstructure). The Ag NPs free cryogel has highly porosity smooth surface with large surface area. Adding Ag NPs decreased these features and increased the 3D roughness. Optimum adsorption of reactive blue 49 was observed after 30 min of contact with 1.5 g/L of the cryogel at pH 1. The adsorption kinetics and isotherm were best described by the pseudo-first-order and Freundlich equations, respectively. All prepared cryogels have notable antibacterial properties that were significantly improved by adding Ag NPs. Overall, the new 3D composite cryogel can efficiently remove dyes and bacteria from wastewater.

The research advances of three dimensional porous cryogel for tissue engineering

Cryogels are a type of hydrogel material which are fabricated by cryopolymerization at subzero temperature. Due to their unique macroporous structure, shape memory properties and injectability, cryogels have gained significant interest in the fields of tissue engineering for encouraging the repair and regeneration of injured tissues. In this review, the basic concepts relevant to cryogels are introduced, and then the fabrication principle, the process parameters and the unique properties of cryogel are discussed. Next, the latest advances of cryogels as three-dimensional scaffold for various tissue engineering applications are given. Finally, this review summarizes the current limitations of cryogels, and strategies to further improve their properties for tissue engineering. The purpose of this article is to provide a reference guide for the researchers in related fields.

Fabrication of lightweight and robust cryogel with opposite wettability for effective oil-water separation via sustainable and toxic-free approach

A porous cryogel with opposing wettability was successfully fabricated using cellulose acetate and polyvinyl alcohol through a simple freeze-drying method for oil-water separation application. It was made by means of water as the medium of mixing to eliminate the use of any hazardous organic solvents which makes it a completely toxic-free and environmentally friendly material. The prepared material was treated with sodium hydroxide (NaOH) to produced hydroxyl groups on the pore structure of the cryogel which led to the increase of its hydrophilicity. On the other hand, methyltrimethoxysilane (MTMS) was used to functionalize the cryogel giving its framework structure an enhanced hydrophobic property. These techniques were carried out to effectively separate water and oil mixture. Moreover, it has high reusability, flexibility, sustainability, durability, and robustness even when exposed to various types of oil due to its exceptional physical properties and is also benign and ecological because of its biodegradability and nontoxicity.

Injectable Glycosaminoglycan-Based Cryogels from Well-Defined Microscale Templates for Local Growth Factor Delivery.

Glycosaminoglycan-based hydrogels hold great potential for applications in tissue engineering and regenerative medicine. By mimicking the natural extracellular matrix processes of growth factor binding and release, such hydrogels can be used as a sustained delivery device for growth factors. Since neural networks commonly follow well-defined, high-aspect-ratio paths through the central and peripheral nervous system, we sought to create a fiber-like, elongated growth factor delivery system. Cryogels, with networks formed at subzero temperatures, are well-suited for the creation of high-aspect-ratio biomaterials, because they have a macroporous structure making them mechanically robust (for ease of handling) yet soft and highly compressible (for interfacing with brain tissue). Unlike hydrogels, cryogels can be synthesized in advance of their use, stored with ease, and rehydrated quickly to their original shape. Herein, we use solvent-assisted microcontact molding to form sacrificial templates, in which we produced highly porous cryogel microscale scaffolds with a well-defined elongated shape via the photopolymerization of poly(ethylene glycol) diacrylate and maleimide-functionalized heparin. Dissolution of the template yielded cryogels that could load nerve growth factor (NGF) and release it over a period of 2 weeks, causing neurite outgrowth in PC12 cell cultures. This microscale template-assisted synthesis technique allows tight control over the cryogel scaffold dimensions for high reproducibility and ease of injection through fine gauge needles.

PH-Dependent Morphology Control of Cellulose Nanofiber/Graphene Oxide Cryogels.

The precise control of the ice crystal growth during a freezing process is of essential importance for achieving porous cryogels with desired architectures. The present work reports a systematic study on the achievement of multi-structural cryogels from a binary dispersion containing 50 wt% 2,2,6,6-tetramethylpiperidin-1-oxyl, radical-mediated oxidized cellulose nanofibers (TOCNs), and 50 wt% graphene oxide (GO) via the unidirectional freeze-drying (UDF) approach. It is found that the increase in the sol's pH imparts better dispersion of the two components through increased electrostatic repulsion, while also causing progressively weaker gel networks leading to micro-lamella cryogels from the UDF process. At the pH of 5.2, an optimum between TOCN and GO self-aggregation and dispersion is achieved, leading to the strongest TOCN-GO interactions and their templating into the regular micro-honeycomb structures. A two-faceted mechanism for explaining the cryogel formation is proposed and it is shown that the interplay of the maximized TOCN-GO interactions and the high affinity of the dispersoid complexes for the ice crystals are necessary for obtaining a micro-honeycomb morphology along the freezing direction. Further, by linking the microstructure and rheology of the corresponding precursor sols, a diagram for predicting the microstructure of TOCN-GO cryogels obtained through the UDF process is proposed.

An ultrasensitive label-free electrochemical immunosensor based on 3D porous chitosan–graphene–ionic liquid–ferrocene nanocomposite cryogel decorated with gold nanoparticles for prostate-specific antigen

A highly sensitive and selective label-free electrochemical immunosensor was successfully fabricated for measuring prostate-specific antigen (PSA). A composite of chitosan, graphene, ionic liquid and ferrocene (CS-GR–IL–Fc) was drop casted onto a screen-printed carbon electrode (SPCE) and frozen to create a layer of 3D porous cryogel (CS-GR-IL-Fc cry) which was decorated with gold nanoparticles (AuNPs). The biocompatibility and porosity of the cryogel increased the surface area available for AuNPs loading via amino groups and the population of anti-PSA, immobilized on the AuNPs via chemisorption, could be increased. The CS-GR-IL-Fc cry displayed excellent conductivity, enhancing electron transfer and amplifying the current signal. Differential pulse voltammetry was employed to determine PSA by measuring the reduction in the Fc oxidation peak current in response to the formation of PSA/anti-PSA immunocomplex. Under the optimized incubation time and electrolyte pH, the developed immunosensor displayed excellent analytical performances, including a wide linear range at concentrations from 1.0 × 10−7 to 1.0 × 10−1 ng mL−1, with a very low limit of detection of 4.8 × 10−8 ng mL−1 and good reproducibility (relative standard deviation of <4.6%, n = 6), stability (90% sensitivity within 20 days), repeatability (12 cycles of binding–rebinding, the sensitivity > 90%) and selectivity. The results obtained from the device for the determination of PSA in human serum were consistent with results from the enzyme-linked immunosorbent assay (P > 0.05), and indicated the promising potential of the proposed immunosensor in clinical diagnosis.

Glucose-responsive shape-memory cryogels

Abstract

Thiol-Reactive Clickable Cryogels: Importance of Macroporosity and Linkers on Biomolecular Immobilization.

Macroporous cryogels that are amenable to facile functionalization are attractive platforms for biomolecular immobilization, a vital step for fabrication of scaffolds necessary for areas like tissue engineering and diagnostic sensing. In this work, thiol-reactive porous cryogels are obtained via photopolymerization of a furan-protected maleimide-containing poly(ethylene glycol) (PEG)-based methacrylate (PEGFuMaMA) monomer. A series of cryogels are prepared using varying amounts of the masked hydrophilic PEGFuMaMA monomer, along with poly(ethylene glycol) methyl ether methacrylate and poly(ethylene glycol) dimethacrylate, a hydrophilic monomer and cross-linker, respectively, in the presence of a photoinitiator. Subsequent activation to the thiol-reactive form of the furan-protected maleimide groups is performed through the retro Diels-Alder reaction. As a demonstration of direct protein immobilization, bovine serum albumin is immobilized onto the cryogels. Furthermore, ligand-directed immobilization of proteins is achieved by first attaching mannose- or biotin-thiol onto the maleimide-containing platforms, followed by ligand-directed immobilization of concanavalin A or streptavidin, respectively. Additionally, we demonstrate that the extent of immobilized proteins can be controlled by varying the amount of thiol-reactive maleimide groups present in the cryogel matrix. Compared to traditional hydrogels, cryogels demonstrate enhanced protein immobilization/detection. Additionally, it is concluded that utilization of a longer linker, distancing the thiol-reactive maleimide group from the gel scaffold, considerably increases protein immobilization. It can be envisioned that the facile fabrication, conjugation, and control over the extent of functionalization of these cryogels will make these materials desirable scaffolds for numerous biomedical applications.

Synthesis, antimicrobial activity, and sustainable release of novel α-aminophosphonate derivatives loaded carrageenan cryogel

Novel α-aminophosphonates 4 were synthesized via one pot three-component reaction of 4-aminoantipyrine, aldehydes, triphenylphosphite and Lewis acid catalyst. The chemical structures of all the synthesized compounds were elucidated by IR, NMR and MS spectral analysis. The antimicrobial activity of 4 was tested in vitro against pathogenic microbes such as E.coli, S.aureus, A.niger and C.albicans. Three of them (4f–h) exhibited high antimicrobial activity and were loaded to carrageenan cryogel for drug delivery studies. With the aid of cellulose nanofibrils (CNF) as reinforcing material and glyoxal as a cross-linking agent, porous cryogels with improved mechanical properties were obtained. Among all, CAR-7 presents the optimum cryogel sample, which contained around 16% CNF and 0.2 mL/15 mL polymer blend. CAR-7 demonstrated highest mechanical compressive strength, porosity (80%), and swelling capacity (75%). Sustainable release behavior over 24 h was observed for the loaded cryogels. The antimicrobial activity of cryogels against S.aureus showed marginal differences between samples. CAR-9 (loaded with 4f) showed the highest reduction percentage in number of bacterial colonies (99.94%) followed by CAR-11 (loaded with 4h, 99.3%) and finally CAR-10 (loaded with 4g, 99.29%).

Exosome laden oxygen releasing antioxidant and antibacterial cryogel wound dressing OxOBand alleviate diabetic and infectious wound healing

Lack of oxygen, reduced vascularization, elevated oxidative stress, and infection are critical clinical hallmarks of non-healing chronic diabetic wounds. Therefore, delivering oxygen, inducing angiogenesis, and management of oxidative stress and infection may provide newer and improved therapeutic avenues for better clinical outcomes in diabetic wound healing. Here, we report the development and evaluation of an exosome laden oxygen releasing antioxidant wound dressing OxOBand to promote wound closure and skin regeneration in diabetic wounds. OxOBand is composed of antioxidant polyurethane (PUAO), as highly porous cryogels with sustained oxygen releasing properties and supplemented with adipose-derived stem cells (ADSCs) exosomes. Exosomes engulfed by the cells enhanced the migration of human keratinocytes and fibroblasts and increased the survival of human neuroblastoma cells under hyperglycemic conditions. OxOBand facilitated faster wound closure, enhanced collagen deposition, faster re-epithelialization, increased neo-vascularization, and decreased oxidative stress within two weeks as compared to untreated diabetic control wounds. The dressing promoted the development of mature epithelial structures with hair follicles and epidermal morphology similar to that of healthy skin. In clinically challenging infected diabetic wounds, these dressings prevented infection and ulceration, improved wound healing with increased collagen deposition, and re-epithelialization. Altogether, OxOBand is a remarkably newer treatment strategy for enhanced diabetic wound healing and may lead to novel therapeutic interventions for the treatment of diabetic ulcers.

Effect of unidirectional freezing using a thermal camera on polyvinyl (alcohol) for aligned porous cryogels

ABSTRACT Unidirectional freeze casting for polymers and ceramics has been performed extensively; however, the mechanisms involved in unidirectional freezing are not thoroughly understood and therefore, in this study, polyvinyl (alcohol) hydrogels were freeze-thawed by unidirectional freezing and the resultant freezing profile was investigated using thermal imagery to comprehend the physical mechanism involved in the production of these aligned cryogels. The approach used follows a slight variation from what is currently used in literature, by enclosing the mould with insulating materials and leaving one side with a metal encasing for a fast freezing flow from the direction of the desired aligned of the structure. This method was cost-effective and simple; however, it was able to induce unidirectional freezing resulting in two distinct structures. These structures exhibited a cellular region interspersed with a fibrous and porous morphology. The method developed without the need of a freeze-dryer was confirmed to produce a unidirectional freezing which can be used for future studies.

Uric acid enzyme biosensor based on a screen-printed electrode coated with Prussian blue and modified with chitosan-graphene composite cryogel

An amperometric uric acid (UA) biosensor was developed by immobilizing uricase on porous cryogel (cry) platform of graphene-incorporated chitosan (Chi) on top of a Prussian blue layer (PB) electrodeposited on a screen-printed carbon electrode (Uricase/Chi-Gr cry/PB/SPCE). Amperometric detection of UA catalyzed by uricase was based on the change in cathodic current of PB at a potential of 0.00 V in a flow injection system. The UA biosensor showed a linear range between 0.0025 and 0.40 mmol L−1 with a detection limit of 2.5 µmol L−1 (S/N = 3). In addition, the Uricase/Chi-Gr cry/PB/SPCE provided an excellent stability that enabled reuse up to 175 times (RSD of 3.7%) and a good electrode-to-electrode repeatability (RSDs of 0.53–2.7%, n = 6). A Michaelis-Menten constant (KMapp) of 0.23 mmol L−1 indicated the excellent affinity of the immobilized uricase toward UA. The modified electrode showed no effect from the common interferences in human serum samples. When applied to measure UA in human serum samples, the developed UA biosensor achieved recoveries ranging from 98 ± 2 to 102 ± 5% and the results obtained were in good agreement with enzymatic colorimetric analysis (P > 0.05).

Highly porous polymer cryogel based tribopositive material for high performance triboelectric nanogenerators

The ongoing research for tribonegative materials has resulted in significant progress in triboelectric nanogenerators (TENG), but the search for tribopositive materials is limited. Therefore, it is still a challenging task to achieve low cost, readily available, easy process for fabrication of tribomaterials with high positive triboelectric properties for triboelectric nanogenerators. Here we propose a novel method to synthesize high porous cryogel films based on facile freezing, ultraviolet radiation and thawing processes and used it as the tribopositive material for TENG fabrication while spin-coated polydimethylsiloxane (PDMS) is used as the tribonegative material. The cryogel/PDMS TENG device with a dimension of 1 × 2 cm2 showed an open circuit voltage of 170 V, and a short circuit current density of 17.1 mA/m2 and an instantaneous power density of 2.95 W/m2 which is able to light up 180 white LEDs directly. Thorough investigations divulged that the high output performance of TENG can be attributed to the porous structure of the cryogel films and the formation of high-density mechanoradicals associated with the porous structure, which was proved by using radical scavanger 2,2,6,6-tetramethyl-1-piperidinyloxy. This study provides a simple, cost-effective and easy way of preparing cryogel films as the tribopositive material for TENGs, which increases the portfolio of tribopositive materials used for triboelectric nanogenerator with prodigious prospects.

The use of M@p(4‐VP) and M@p (VI) (M:Co, Ni, Cu) cryogel catalysts as reactor in a glass column in the reduction of p‐nitrophenol to p‐aminophenol under gravity

AbstractIn this study, metal@p(4‐Vinyl pyridine) (M@p(4‐VP)) and metal@p (vinyl imidazole) (M@p (VI)) (M: Co, Ni, and Cu) cryogel catalysts as continuous reactor were used in reduction of p‐nitrophenol (p‐NP) to p‐amino phenol (p‐AP). The aqueous solution of p‐NP passed through these super porous cryogel catalysts under gravity, and reaction kinetic for p‐AP generation was investigated. The effect of metal species, amount of p‐NP solution, amount of metal nanoparticle (increased by loading/reducing cycles), and temperature on the reduction reaction for p‐NP to p‐AP catalyzed by M@p(4‐VP) and M@p (VI) cryogel catalysts were investigated. It was found that Co@p(4‐VP) and Co@p (VI) cryogel catalysts provided the best catalytic performances than the other used metal species with 2.19 and 1.37 mol p‐NP/(mol catalyst.min)−1 turn‐over frequency (TOF) values. Furthermore, in addition better TOF values, low Ea values of the prepared Co@p(4‐VP) and Co@p (VI) cryogel catalysts with the values of 18.9 ± 1.3, and 25.4 ± 1.8 kJ/mol were found to be better than most of the similar works reported in the literature.

Quanfima: An open source Python package for automated fiber analysis of biomaterials.

Hybrid 3D scaffolds composed of different biomaterials with fibrous structure or enriched with different inclusions (i.e., nano- and microparticles) have already demonstrated their positive effect on cell integration and regeneration. The analysis of fibers in hybrid biomaterials, especially in a 3D space is often difficult due to their various diameters (from micro to nanoscale) and compositions. Though biomaterials processing workflows are implemented, there are no software tools for fiber analysis that can be easily integrated into such workflows. Due to the demand for reproducible science with Jupyter notebooks and the broad use of the Python programming language, we have developed the new Python package quanfima offering a complete analysis of hybrid biomaterials, that include the determination of fiber orientation, fiber and/or particle diameter and porosity. Here, we evaluate the provided tensor-based approach on a range of generated datasets under various noise conditions. Also, we show its application to the X-ray tomography datasets of polycaprolactone fibrous scaffolds pure and containing silicate-substituted hydroxyapatite microparticles, hydrogels enriched with bioglass contained strontium and alpha-tricalcium phosphate microparticles for bone tissue engineering and porous cryogel 3D scaffold for pancreatic cell culturing. The results obtained with the help of the developed package demonstrated high accuracy and performance of orientation, fibers and microparticles diameter and porosity analysis.