Porcine Red Blood Cells Research Articles

Pathological and virological observations on canine parvoviral enteritis and myocarditis in the Wellington region

Abstract Five outbreaks of acute enteritis and one of myocarditis in 5 to 12-week-old puppies are reported. In cases of enteritis the initial clinical finding of slight malaise was followed within 24 hours by severe vomiting, diarrhoea, dehydration and death in most cases. The lesions of acute necrotizing enteritis and widespread lymphoid depletion were identical to those of feline panleucopaenia. In the outbreak of myocarditis, 3 puppies were founddead and a fourth developed congestive heart failure. Parvoviral-like particles were found in the faeces of 5 cases of enteritis by electron microscopy and virus was isolated from 2 dogs, using a feline kidney cell line. The isolates demonstrated the physicochemical characteristics of a parvovirus and haemagglutinated porcine red blood cells at 4°C and 22°C but not 37°C. The isolates were antigenically related to feline panleucopaenia virus by immune aggregation, haemagglutination inhibition and immunofluorescence. Fourteen of 48 sera from local dogs had signif...

Protein concentrates from slaughter animal blood

SummaryPurified protein concentrates, containing 95 and 98% protein (dry weight basis), were prepared from centrifugally separated porcine red blood cells by dilution with deionized water followed by either ultrafiltration or diafiltration. Optimal operating conditions, in terms of temperature, pressure and flow rate, for membrane processing of red blood cells in a plate and frame RO/UF plant were established. Permeation rate during ultrafiltration of red blood cells decreased with increasing protein concentration. Protein concentration of up to a maximum of 30% (liquid basis) was achieved by simple ultrafiltration. During diafiltration permeation rate increased with decreasing levels of non‐protein material in the retentate. Mass balance equations were developed to allow ultrafiltration and diafiltration process times to be calculated for pre‐specified degrees of protein purification. the purification of red blood cells was better achieved by dilution followed by diafiltration at low protein levels. the optimal protein content for diafiltration will depend on the intended post‐purification use of the cell concentrate.