Protein concentrates from slaughter animal blood

Abstract

SummaryPurified protein concentrates, containing 95 and 98% protein (dry weight basis), were prepared from centrifugally separated porcine red blood cells by dilution with deionized water followed by either ultrafiltration or diafiltration. Optimal operating conditions, in terms of temperature, pressure and flow rate, for membrane processing of red blood cells in a plate and frame RO/UF plant were established. Permeation rate during ultrafiltration of red blood cells decreased with increasing protein concentration. Protein concentration of up to a maximum of 30% (liquid basis) was achieved by simple ultrafiltration. During diafiltration permeation rate increased with decreasing levels of non‐protein material in the retentate. Mass balance equations were developed to allow ultrafiltration and diafiltration process times to be calculated for pre‐specified degrees of protein purification. the purification of red blood cells was better achieved by dilution followed by diafiltration at low protein levels. the optimal protein content for diafiltration will depend on the intended post‐purification use of the cell concentrate.